WO2013048163A3 - Convenient and efficient purification method for chemically labeled nucleic acids - Google Patents

Convenient and efficient purification method for chemically labeled nucleic acids Download PDF

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Publication number
WO2013048163A3
WO2013048163A3 PCT/KR2012/007870 KR2012007870W WO2013048163A3 WO 2013048163 A3 WO2013048163 A3 WO 2013048163A3 KR 2012007870 W KR2012007870 W KR 2012007870W WO 2013048163 A3 WO2013048163 A3 WO 2013048163A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
labeled nucleic
convenient
unreacted
purifying
Prior art date
Application number
PCT/KR2012/007870
Other languages
French (fr)
Other versions
WO2013048163A2 (en
Inventor
Seong Keun Kim
Young Gyu Kim
Ji Hee Hwang
Original Assignee
Snu R&Db Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020120104438A external-priority patent/KR101446316B1/en
Application filed by Snu R&Db Foundation filed Critical Snu R&Db Foundation
Publication of WO2013048163A2 publication Critical patent/WO2013048163A2/en
Publication of WO2013048163A3 publication Critical patent/WO2013048163A3/en
Priority to US14/228,570 priority Critical patent/US20140235842A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention relates to a method for purifying chemically labeled nucleic acids. More particularly, the present invention relates to a convenient and efficient method for purifying labeled nucleic acids in a fast manner with high efficiency from a mixture of unreacted hydrophobic probes, unreacted nucleic acids and labeled nucleic acids. In particular, the present invention relates to a convenient method for purifying labeled nucleic acids in a fast manner with high efficiency from a mixture of unreacted hydrophobic chemical probes, unreacted nucleic acids and labeled nucleic acids, comprising the steps of reacting hydrophobic chemical probe and nucleic acids in an aqueous solution to prepare a reacted solution; adding organic solvent to the reacted solution and stirring it to obtain a mixed solution; and centrifuging the mixed solution and removing organic solvent phase.
PCT/KR2012/007870 2011-09-29 2012-09-27 Convenient and efficient purification method for chemically labeled nucleic acids WO2013048163A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/228,570 US20140235842A1 (en) 2011-09-29 2014-03-28 Convenient and efficient purification method for chemically labeled nucleic acids

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR10-2011-0099061 2011-09-29
KR20110099061 2011-09-29
KR10-2012-0020649 2012-02-08
KR20120020649 2012-02-28
KR1020120104438A KR101446316B1 (en) 2011-09-29 2012-09-20 Convenient and efficient purification method for chemically labeled nucleic acids
KR10-2012-0104438 2012-09-20

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US14/228,570 Continuation US20140235842A1 (en) 2011-09-29 2014-03-28 Convenient and efficient purification method for chemically labeled nucleic acids

Publications (2)

Publication Number Publication Date
WO2013048163A2 WO2013048163A2 (en) 2013-04-04
WO2013048163A3 true WO2013048163A3 (en) 2013-05-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/007870 WO2013048163A2 (en) 2011-09-29 2012-09-27 Convenient and efficient purification method for chemically labeled nucleic acids

Country Status (1)

Country Link
WO (1) WO2013048163A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185439A (en) * 1987-10-05 1993-02-09 Gen-Probe Incorporated Acridinium ester labelling and purification of nucleotide probes
US6818760B1 (en) * 1999-10-06 2004-11-16 Prolinx Incorporated Removal of dye-labeled dideoxy terminators from DNA sequencing reactions
US20100278745A1 (en) * 2006-12-21 2010-11-04 Norbert Lange Compounds for fluorescence imaging

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185439A (en) * 1987-10-05 1993-02-09 Gen-Probe Incorporated Acridinium ester labelling and purification of nucleotide probes
US6818760B1 (en) * 1999-10-06 2004-11-16 Prolinx Incorporated Removal of dye-labeled dideoxy terminators from DNA sequencing reactions
US20100278745A1 (en) * 2006-12-21 2010-11-04 Norbert Lange Compounds for fluorescence imaging

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SERGEY SOLOMATIN ET AL.: "Methods of site-specific labeling of RNA with fluorescent dyes", METHODS IN ENZYMOLOGY, vol. 469, 17 November 2009 (2009-11-17), pages 47 - 68, XP003031631 *
XIAOLI SUN ET AL.: "Development of SNAP-Tag fluorogenic probes for wash-free fluorescence imaging", CHEMBIOCHEM, vol. 12, no. 14, 19 September 2011 (2011-09-19), pages 2217 - 2226, XP055069445 *

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Publication number Publication date
WO2013048163A2 (en) 2013-04-04

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