WO2013047611A1

WO2013047611A1 - Packaged beverage

Info

Publication number: WO2013047611A1
Application number: PCT/JP2012/074773
Authority: WO
Inventors: 俊迫田
Original assignee: 花王株式会社
Priority date: 2011-09-27
Filing date: 2012-09-26
Publication date: 2013-04-04
Also published as: JP5298232B2; JP2013081453A

Abstract

Provided is a packaged beverage containing isoquercitrin and a sugar adduct thereof, said beverage not changing color easily even if stored for a long period. This packaged beverage contains the following components (A) and (B): (A) 0.03 to 0.17 mass% of isoquercitrin and sugar adduct thereof; and (B) 0.01 to 3 mass% of sugar alcohol and/or trehalose (B). The mass ratio [(B)/(A)] of component (B) to component (A) is 3 to 50, and the pH is 2 to 5.

Description

Container drink

The present invention relates to a packaged beverage.

Isoquercitrin is a flavonol glycoside in which one glucose is β-bonded at the 3-position of quercetin. Isoquercitrin has a strong antioxidant activity and is known to prevent discoloration of the pigment (Patent Document 1), and is also expected to act on the living body such as anti-arteriosclerosis and blood flow improvement. ing.

However, isoquercitrin is poorly soluble in water and thus works effectively in vitro, but is not sufficiently effective in vivo and has limited use as a water-based composition for beverages and the like. Therefore, in order to improve the solubility in water, “enzyme-treated isoquercitrin” obtained by allowing glycosyltransferase to act on isoquercitrin in the presence of starch is proposed (Patent Document 2). “Enzyme-treated isoquercitrin” is a mixture of isoquercitrin and α-glucosylisoquercitrin in which one or more glucoses are further added to the glucose residue of isoquercitrin with an α-1,4 bond.

Also proposed is a quercetin glycoside composition in which the composition of α-glucosyl isoquercitrin contained in enzyme-treated isoquercitrin is changed by fractionation to further enhance oral absorbability (Patent Document 3). Recently, it has been found that isoquercitrin has an action to reduce body fat of obese people, and it is reported that it is effective when ingested in the form of a beverage (Non-patent Document 1).

JP 2008-131888 A JP-A-1-213293 International Publication No. 2006/070883 Pamphlet

The present invention includes the following components (A) and (B):
(A) Isoquercitrin and its sugar adduct 0.03-0.17% by mass,
(B) At least one selected from sugar alcohol and trehalose 0.01 to 3% by mass
Containing
The present invention provides a packaged beverage having a mass ratio [(B) / (A)] of component (B) to component (A) of 3 to 50 and a pH of 2 to 5.

In order to express the physiological action of isoquercitrin more effectively, it is effective to add sugar to isoquercitrin by enzyme treatment or the like to increase its intake and absorption by the body. As a means that can be easily achieved, there is a form of a beverage.
However, since polyphenols such as flavonol glycosides are generally easily oxidized, when a beverage containing them is stored for a long period of time, the coloration gradually proceeds and the color tone changes greatly.
The present inventor has found that the color change becomes apparent as the enzyme-treated isoquercitrin is blended in a beverage and the concentration thereof is increased. In addition, even when the enzyme-treated isoquercitrin is contained at a high concentration, it has been found that no change in color tone is observed in a low moisture food or a high viscosity food such as jelly. Thus, in food and drink containing enzyme-treated isoquercitrin, it has been found that color change is a problem specific to the form of a low-viscosity beverage.

The present invention is to provide a container-packed beverage containing isoquercitrin and a sugar adduct thereof that hardly change in color tone even when stored for a long period of time.

The present inventor contains sugar alcohol or trehalose in a specific amount together with isoquercitrin and its sugar adduct, and further adjusts the pH within a specific range, so that the color tone hardly changes even when stored for a long period of time. It has been found that a beverage having an external appearance can be obtained.

According to the present invention, it is possible to provide a packaged beverage containing isoquercitrin and a sugar adduct thereof that hardly change in color tone even when stored for a long period of time.

The container-packed beverage of the present invention contains 0.03 to 0.17% by mass of (A) isoquercitrin and a sugar adduct thereof (hereinafter also referred to as “isoquercitrin sugar adduct etc.”). The content of the component (A) is preferably 0.15% by mass or less, more preferably 0.12% by mass or less, and further preferably 0.1% by mass or less from the viewpoint of suppressing color change. Moreover, content of a component (A) is 0.04 mass% or more from a viewpoint of a physiological effect, Furthermore, 0.05 mass% or more, Furthermore, 0.06 mass% or more is preferable. The range of the content of component (A) is 0.04 to 0.15% by mass, further 0.05 to 0.12% by mass, further 0.06 to 0.12% by mass, and further 0.06 to 0%. .1% by mass is preferable.

Isoquercitrin is obtained by β-bonding one glucose at the 3-position of quercetin (quercetincet3-O-β-D-glucopyranoside). The isoquercitrin sugar adduct can be obtained, for example, by allowing glycosyltransferase to act on isoquercitrin in the presence of a sugar donor (glucose source), and adding one or more glucose to the glucose residue of isoquercitrin. α-Glucosylisoquercitrin further added with α-1,4 bond (FFI Journal, Vol.209, No.7, 2004, p.622-628; Food Hygiene Journal, Vol.41, No. .1, p.54-60).

More specifically, the component (A) includes isoquercitrin in which the number of glucose residues (n) is 0 in the following formula (1) and an integer in which the number of glucose residues (n) is 1 or more (preferably A mixture with an isoquercitrin sugar adduct which is an integer of 1 to 15, more preferably an integer of 1 to 10, and still more preferably an integer of 1 to 8.

[Wherein Glc represents a glucose residue, and n represents 0 or an integer of 1 or more. ]

Also, the mass ratio of isoquercitrin sugar adduct / isoquercitrin in component (A) is preferably 1 or more, more preferably 2 or more, and particularly preferably 4 or more, from the viewpoint of water solubility. The mass ratio is preferably 1000 or less, more preferably 500 or less, further 100 or less, further 50 or less, further 25 or less, and particularly 10 or less from the viewpoint of production efficiency. The range of the mass ratio is preferably 1 to 1000, further 1 to 500, further 1 to 100, further 1 to 50, further 1 to 25, further 1 to 10, further 2 to 10, and further 4 to 10.

Such (A) isoquercitrin sugar adducts and the like are commercially available. For example, Sanmerin AO-1007, Sanmerin Powder C-10 and the like (manufactured by Saneigen FFI Co., Ltd.) Can be mentioned.

Examples of glycosyltransferases used for glycosylation of isoquercitrin include glucosidases such as α-amylase (EC 3.2.1.1) and α-glucosidase (EC 3.2.1.20), and cyclodextrin glucanotransferase (EC). And transglucosidase such as 2.4.1.19).

These glycosyltransferases are all commercially available, and examples thereof include contizyme (manufactured by Amano Enzyme). The amount of glycosyltransferase used is not uniform depending on the type of enzyme and the desired composition. For example, cyclodextrin glucanotransferase [enzyme specific activity of about 100 units (β-cyclodextrin from soluble starch per 1 minute per minute) The amount of enzyme produced is defined as 1 unit)] is usually 0.001 to 20 parts by weight, preferably 0.005 to 10 parts by weight, more preferably 0.01 to 10 parts by weight per 1 part by weight of isoquercitrin. 5 parts by mass.

The sugar donor (glucose source) used for glycosylation is not particularly limited as long as one or more molecules of the glucose residue can be transferred to one molecule of isoquercitrin. Glucose, maltose, amylose, amylopectin, starch, starch liquefied product, starch saccharified product, cyclodextrin and the like. The amount of glucose used is usually 0.1 to 20 parts by weight, preferably 0.5 to 15 parts by weight, and more preferably 1 to 10 parts by weight with respect to 1 part by weight of isoquercitrin.

Further, the composition of the resulting (A) isoquercitrin sugar adduct is not uniform depending on the reaction conditions. For example, the temperature is 80 ° C. or less, preferably 20 to 80 ° C., more preferably 40 to 75 ° C., pH 3 to 11 In addition, various compositions can be obtained by allowing glycosyltransferase to act on isoquercitrin in the presence of a sugar donor (glucose source), preferably under conditions of pH 4-8. In addition, the analysis method of (A) isoquercitrin sugar adduct and the like shall follow the method described in the examples described later.

The above reaction can be performed while standing or stirring or shaking. In order to prevent oxidation during the reaction, the head space of the reaction system may be replaced with an inert gas such as nitrogen, and an antioxidant such as ascorbic acid may be added to the reaction system.

(A) Isoquercitrin sugar adducts and the like can be prepared starting from rutin as well as using isoquercitrin as a raw material as described above. For example, α-1,6-rhamnosidase (EC 3.2.1.40) is allowed to act on rutin to convert it to isoquercitrin, and then (A) an isoquercitrin sugar adduct is prepared according to the above-mentioned method. it can. α-1,6-rhamnosidase is commercially available, and examples thereof include hesperidinase, naringinase (manufactured by Tanabe Seiyaku Co., Ltd.), and cellulase A “Amano” 3 (manufactured by Amano Enzyme).

In the present invention, in the (A) isoquercitrin sugar adduct represented by the above formula (1), isoquercitrin in which n is 0 and isoquercitrin sugar addition in which n is an integer of 1 or more For example, isoquercitrin sugar adducts where n is 0 and isoquercitrin sugar adducts where n is 4 or more are reduced by fractionation and rich in isoquercitrin sugar adducts where n is 3 The fraction contained in may be collected and used.

The packaged beverage of the present invention preferably contains (A) an isoquercitrin sugar adduct or the like so as to be 0.01 to 0.07% by mass in terms of quercetin from the viewpoint of suppressing color change, and further 0 0.06% by mass or less, more preferably 0.05% by mass or less, and further preferably 0.04% by mass or less, and from the viewpoint of physiological effects, 0.016% by mass or more, further 0.021% by mass or more, and further 0.025 mass% or more is preferable. The content range of the component (A) is preferably 0.016 to 0.06% by mass, more preferably 0.021 to 0.05% by mass, and further preferably 0.025 to 0.04% by mass in terms of quercetin.

In addition, the container-packed beverage of the present invention contains at least one selected from (B) sugar alcohol and trehalose in order to suppress color change. Examples of the sugar alcohol include erythritol, sorbitol, xylitol, maltitol, lactitol, mannitol, threitol, arabinitol, ribitol and the like. Of these, erythritol, xylitol, and maltitol are preferable. Sugar alcohol can be used individually or in combination of 2 or more types. The component (B) is preferably at least one selected from erythritol, xylitol, maltitol, and trehalose, and more preferably at least one selected from erythritol, xylitol, and trehalose from the viewpoint of further suppressing color change.

The content of the component (B) in the packaged beverage of the present invention is 0.01 to 3% by mass, preferably 2% by mass or less, more preferably 1.5% from the viewpoint of further suppressing color change. % By mass or less, preferably 0.05% by mass or more, more preferably 0.1% by mass or more, still more preferably 0.2% by mass or more, still more preferably 0.3% by mass or more, and even more preferably 0%. 4% by mass or more. The range of the content of the component (B) is from 0.05 to 3% by mass, further from 0.1 to 2% by mass, further from 0.2 to 1.5% by mass, and further preferably from the viewpoint of suppressing color change. It is preferably 3 to 1.5% by mass, more preferably 0.4 to 1.5% by mass.

The mass ratio [(B) / (A)] of the component (A) and the component (B) in the packaged beverage of the present invention is preferably 50 or less from the viewpoints of color change suppression, flavor, and over the throat. More preferably, it is 40 or less, More preferably, it is 30 or less, More preferably, it is 25 or less, More preferably, it is 20 or less, Preferably it is 3 or more, More preferably, it is 5 or more, More preferably, it is 7 or more. The range of the mass ratio [(B) / (A)] is preferably 3 to 50, more preferably 3 to 40, further 5 to 30, and more preferably 7 to 30. Further, from the viewpoint of color change suppression and flavor, the mass ratio [(B) / (A)] is preferably 5 to 25, more preferably 7 to 20.

Furthermore, the packaged beverage of the present invention may contain a sweetener excluding sugar alcohol and trehalose. Examples of such sweeteners include crystalline sugars such as monosaccharides such as fructose, glucose, tagatose and arabinose, disaccharides such as lactose, maltose and sucrose, and polysaccharides such as powdered syrup, oligosaccharides such as maltooligosaccharide and galactooligosaccharide. Examples thereof include non-crystalline saccharides such as sugar and syrup, and honey and the like in which sugars such as glucose and fructose account for about 70%. These sweeteners can be used alone or in combination of two or more.
The content of sweeteners (excluding sugar alcohol and trehalose) in the packaged beverage of the present invention can be determined as appropriate, but is 0.02 to 1% by mass, and further 0.05 to 0.8% by mass. Further, 0.05 to 0.6% by mass is preferable from the viewpoint of suppressing color tone change.

Moreover, in this invention, it can also be set as a carbonated drink depending on necessity, and a soft feeling and a refreshing feeling can be provided continuously by the moderate foaming property of a carbon dioxide gas, and palatability can be improved.
When carbon dioxide is contained in the packaged beverage of the present invention, the content is preferably 1% by mass or less, more preferably 0.1 to 0.9% by mass, and still more preferably 0.2 to 0.8% by mass. %, Particularly preferably 0.3 to 0.7% by weight. In addition, as a measuring method of a carbon dioxide gas, it can measure with a gas volume meter and can convert into gas amount. The gas volume refers to the ratio of the gas volume at 20 ° C. of the carbon dioxide dissolved in the beverage to the beverage volume. In the case of the packaged beverage of the present invention, preferably 0.5 to 4, and more preferably 1 to 3. 8, more preferably 2 to 3.5.

Furthermore, the container-packed drink of this invention can contain a potassium ion. Thereby, the refreshing feeling is not diluted even during long-term storage, and the refreshing feeling in the mouth is maintained. Therefore, it is suitable not only for drinking in daily life, but also as a drink that is drunk particularly in sports.
Potassium ions as potassium salts such as potassium chloride, potassium carbonate, potassium sulfate, potassium acetate, potassium bicarbonate, potassium citrate, potassium phosphate, potassium hydrogen phosphate, potassium tartrate, potassium sorbate etc. or mixtures thereof Can be blended. The potassium ions include those derived from raw materials.
The content of potassium ions in the packaged beverage of the present invention is preferably 0.001% by mass or more, more preferably 0.002% by mass or more, still more preferably 0.003% by mass or more, and further preferably 0.005. % By mass or more, particularly preferably 0.01% by mass or more, and preferably 0.2% by mass or less, more preferably 0.1% by mass or less, still more preferably 0.09% by mass or less, still more preferably 0%. 0.08% by mass or less, particularly preferably 0.07% by mass or less. The range of the potassium ion content is preferably 0.001 to 0.2% by mass, more preferably 0.002 to 0.1% by mass, further 0.003 to 0.09%, and further 0.005 to 0.08. % By mass, especially 0.01 to 0.07% by mass is preferred.

Furthermore, in the packaged beverage of the present invention, as desired, acidulant, vitamin, flavor (including emulsified flavor), mineral, antioxidant, foaming agent, foam stabilizer, various esters, pigments, emulsifier, Additives such as preservatives, seasonings, vegetable extracts, nectar extracts, and quality stabilizers can be contained alone or in combination of two or more. In addition, content of an additive can be suitably selected within the range which does not prevent the objective of this invention.

The pH (20 ° C.) of the packaged beverage of the present invention is 2 to 5, but 3 to 4 is preferable from the viewpoint of further suppressing color tone change.

The container-packed beverage of the present invention preferably has a low viscosity in a container-packed state. For example, it is preferably not a high-viscosity food having shape-retaining properties such as jelly, but a viscosity that allows it to flow and be drunk only by tilting the container. The specific viscosity is preferably 0.01 to 500 mPa · s, more preferably 0.01 to 400 mPa · s, and particularly preferably 0.01 to 200 mPa · s when measured at 20 ° C. with a vibration viscometer. .

Further, the container-packed beverage of the present invention has a turbidity (25 ° C.) of preferably 0.01 to 90, more preferably 0.1 to 85, still more preferably 0.1 to 80, from the viewpoint of color stability. More preferably, it is 0.2 to 75. Here, “turbidity” is a value measured in a state in which transmitted light and scattered light transmitted through a glass cell having an optical path length of 10 mm are combined, and the measuring method is a method described in the examples described later. Shall be followed. When the turbidity is within the above range, it indicates that the components are uniformly dispersed and is suitable for long-term drinking. Even in the form of an oxygen-permeable transparent container, the appearance of the beverage does not change when illuminated by a show window, and the color stability by light irradiation under oxygen transmission is excellent.

Furthermore, the container-packed beverage of the present invention has a value obtained by subtracting the b ^* value of the packaged beverage immediately after production from the b ^* value of the packaged beverage after storage at 55 ° C. for 7 days from the viewpoint of color stability ( Δb ^* ) is preferably less than 2, more preferably 1.9 or less, and further 1.8 or less. Here, the “b ^* value” is one of coordinate values representing hue and saturation when the color is expressed in the L ^* a ^* b ^* color system, and is a coordinate indicating the saturation in the yellow direction. Value. The L ^* a ^* b ^* color system includes L ^* indicating lightness and a ^* which is a coordinate value indicating saturation in the red direction, but in the present invention, it is most easily manifested when the color tone changes. It prescribes about b ^* . The b ^* value is measured according to the method described in the examples below.

The container-packed beverage of the present invention can be produced by, for example, blending the component (A) and the component (B) and adjusting the concentration and pH of each component.

Moreover, as a container which can be used for the container-packed beverage of the present invention, a molded container mainly composed of polyethylene terephthalate (so-called PET bottle), a metal can, a paper container combined with a metal foil or a plastic film, a bottle or the like is used. A packaging container is mentioned.
Furthermore, after filling the container, for example, when heat sterilization such as a metal can can be performed, it can be sterilized under the conditions stipulated in the applicable regulations (the Food Sanitation Law in Japan). On the other hand, those that cannot be sterilized by retort, such as PET bottles and paper containers, are preliminarily sterilized at a high temperature and short time using a plate heat exchanger, for example, and then cooled to a certain temperature and filled into containers. Etc. can be adopted. Moreover, you may mix | blend another component with the filled container under aseptic conditions.

The present invention discloses the following invention concerning the above-mentioned embodiment.
[1]
The following components (A) and (B):
(A) Isoquercitrin and its sugar adduct 0.03-0.17% by mass,
(B) At least one selected from sugar alcohol and trehalose 0.01 to 3% by mass
A containerized beverage having a mass ratio [(B) / (A)] of component (B) to component (A) of 3 to 50 and a pH of 2 to 5.
[2]
The content of the component (A) is preferably 0.04% by mass or more, more preferably 0.05% by mass or more, still more preferably 0.06% by mass or more, preferably 0.15% by mass or less, More preferably, it is 0.12 mass% or less, More preferably, it is 0.1 mass% or less, The container-packed drink of said [1] description.
[3]
The content of component (A) is preferably 0.04 to 0.15% by mass, more preferably 0.05 to 0.12% by mass, still more preferably 0.06 to 0.12% by mass, still more preferably The container-packed beverage according to [1], which is 0.06 to 0.1% by mass.
[4]
In the component (A), the mass ratio of isoquercitrin sugar adduct / isoquercitrin is preferably 1 or more, more preferably 2 or more, further preferably 4 or more, preferably 1000 or less, more preferably 500. In the following, the packaged beverage according to any one of [1] to [3], more preferably 100 or less, further preferably 50 or less, more preferably 25 or less, and still more preferably 10 or less.
[5]
Component (A) has an isoquercitrin sugar adduct / isoquercitrin mass ratio of preferably 1 to 1000, more preferably 1 to 500, still more preferably 1 to 100, still more preferably 1 to 50, and still more preferably. 1 to 25, more preferably 1 to 10, more preferably 2 to 10, and further preferably 4 to 10, the packaged beverage according to any one of [1] to [3] above.
[6]
The content of the component (A) is preferably 0.01 to 0.07% by mass, more preferably 0.016 to 0.06% by mass, and still more preferably 0.021 to 0.05% by mass in terms of quercetin. More preferably, the packaged beverage according to any one of [1] to [5], which is 0.025 to 0.04% by mass.
[7]
The packaged beverage according to any one of [1] to [6] above, wherein the component (B) is at least one selected from erythritol, xylitol, maltitol, and trehalose.
[8]
The packaged beverage according to any one of [1] to [6] above, wherein the component (B) is at least one selected from erythritol, xylitol, and trehalose.
[9]
The content of the component (B) is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, further preferably 0.2% by mass or more, further preferably 0.3% by mass or more, and further preferably Is 0.4 mass% or more, preferably 2 mass% or less, and more preferably 1.5 mass% or less. The packaged beverage according to any one of [1] to [8] above.
[10]
The content of the component (B) is preferably 0.05 to 3% by mass, more preferably 0.1 to 2% by mass, still more preferably 0.2 to 1.5% by mass, still more preferably 0.3 to 3% by mass. The packaged beverage according to any one of [1] to [8], which is 1.5% by mass, and further 0.4 to 1.5% by mass.

[11]
The mass ratio [(B) / (A)] of the component (A) and the component (B) is preferably 3 or more, more preferably 5 or more, still more preferably 7 or more, and preferably 50 or less. The container-packed beverage according to any one of [1] to [10], more preferably 30 or less, still more preferably 25 or less, and still more preferably 20 or less.
[12]
The mass ratio [(B) / (A)] of the component (A) and the component (B) is preferably 3 to 50, more preferably 3 to 40, still more preferably 5 to 30, and still more preferably 7 The container-packed beverage according to any one of [1] to [10], wherein
[13]
The content ratio [(B) / (A)] of the component (A) and the component (B) is preferably 5 to 25, more preferably 7 to 20, of the above [1] to [10] The container-packed drink as described in any one.
[14]
The content of sweeteners other than sugar alcohol and trehalose is preferably 0.02 to 1% by mass, more preferably 0.05 to 0.8% by mass, and still more preferably 0.05 to 0.6% by mass. The packaged beverage according to any one of [1] to [13].
[15]
Further, carbon dioxide is contained, and the content of carbon dioxide is preferably 1% by mass or less, more preferably 0.1 to 0.9% by mass, still more preferably 0.2 to 0.8% by mass, and still more preferably. The packaged beverage according to any one of [1] to [14], which is 0.3 to 0.7% by mass.
[16]
The packaged beverage contains potassium ions, and the content of potassium ions is preferably 0.001% by mass or more, more preferably 0.002% by mass or more, still more preferably 0.003% by mass or more, and further preferably 0. 0.005% by mass or more, more preferably 0.01% by mass or more, preferably 0.2% by mass or less, more preferably 0.1% by mass or less, still more preferably 0.09% by mass or less, still more preferably. Is a packed beverage according to any one of the above [1] to [15], which is 0.08% by mass or less, more preferably 0.07% by mass or less.
[17]
The packaged beverage contains potassium ions, and the content of potassium ions is preferably 0.001 to 0.2% by mass, more preferably 0.002 to 0.1% by mass, and still more preferably 0.003 to 0%. 0.09% by mass, more preferably 0.005 to 0.08% by mass, and still more preferably 0.01 to 0.07% by mass, according to any one of [1] to [15] above .
[18]
The packaged beverage according to any one of [1] to [17], wherein the pH (20 ° C) is preferably 3 to 4.
[19]
The turbidity (25 ° C.) is preferably 0.01 to 90, more preferably 0.1 to 85, still more preferably 0.1 to 80, and still more preferably 0.2 to 75. [18] The packaged beverage according to any one of [18].
[20]
The value (Δb ^* ) obtained by subtracting the b ^* value of the packaged beverage immediately after production from the b ^* value of the packaged beverage after storage at 55 ° C. for 7 days is preferably less than 2, more preferably 1.9 or less. More preferably, the container-packed beverage according to any one of [1] to [19], which is 1.8 or less.

1. Analysis of isoquercitrin sugar adducts etc. The analysis of isoquercitrin sugar adducts etc. was performed by HPLC (high performance liquid chromatograph) method according to the following method.
LC-10AD (manufactured by Shimadzu Corporation) was used as an analytical instrument.
The apparatus configuration of the analytical instrument is as follows.
Detector: UV-visible spectrophotometer SPD-10AV (manufactured by Shimadzu Corporation)
Column: YMC-Pack ODS-A AA12S05-1506WT, φ6mm x 150mm (manufactured by YMC)

The analysis conditions are as follows.
Column temperature: 40 ° C
Mobile phase: Mixture of water, acetonitrile, 2-propanol and acetic acid (200: 38: 2: 1)
Flow rate: 1.0 mL / min
Sample injection volume: 10 μL
Measurement wavelength: 360 nm

A sample for analysis was prepared by the following procedure.
1 g of a sample was weighed, 1 mL of methanol was added, and a mixed solution of methanol and water (1: 1, volume ratio) was further added to make a constant volume of 10 mL to obtain a sample solution. The prepared sample solution was subjected to high performance liquid chromatographic analysis.

In addition, a standard solution of isoquercitrin is prepared, a calibration curve is prepared by subjecting it to high performance liquid chromatographic analysis, and isoquercitrin sugar in the sample solution is prepared using isoquercitrin as an index. Quantification of adducts and the like was performed. That is, from the calibration curve, the molar concentration is determined for each of the sugar adducts of isoquercitrin (n = 0 in the formula (1)) and n ≧ 1 in the HPLC analysis of the sample solution, and further from the molecular weight of each substance. The content (mass%) was calculated, and the amount of isoquercitrin sugar adducts in the sample was determined. In addition, the quercetin equivalent amount of isoquercitrin sugar adducts and the like in the sample was calculated by calculating the content of the aglycone part from the respective molar concentrations of isoquercitrin and each sugar adduct.

2. Analysis of sugar alcohol and trehalose Sugar alcohol was analyzed by HPLC (high performance liquid chromatograph) method according to the following method.
The apparatus configuration of the analytical instrument is as follows.
Detector: Differential refractometer RID-10A (manufactured by Shimadzu Corporation)
Column: Shodex Asahipak NH2P-50 4E, φ4.6mm × 250mm (manufactured by Showa Denko)

The analysis conditions are as follows.
Column temperature: Room temperature Mobile phase: Mixture of acetonitrile and water (81:19 volume ratio)
Flow rate: 1 mL / min
Sample injection volume: 20 μL

A sample for analysis was prepared by the following procedure.
3 g of the sample was weighed, and 10 mL of water was added to dissolve and neutralize the solution, and ultrasonic extraction was performed for 30 minutes using an ultrasonic cleaner. Water was added to the solution to make up to 20 mL. The solution was filtered with a membrane filter to obtain a sample solution. The sample solution was subjected to high performance liquid chromatographic analysis.

3. Analysis of Potassium Ion Potassium is “Explanation of the Manual for Analyzing the Five Standards of Japanese Food Standards Constructed by Analyzing Practitioners” (edited by the Japan Food Analysis Center, published by Chuo Law Publishing Co., Ltd., July 10, 2001) ) Of p90-91 and p99-103.
Specifically, 2 to 5 g of the sample was weighed into an extraction container, 200 mL of 1% hydrochloric acid solution was added, and the mixture was extracted by shaking at room temperature for 30 minutes. The extract was transferred to a centrifuge tube, and the supernatant after centrifugation was used as a test solution for atomic absorption. The measurement wavelength of the atomic absorption photometer was set to 766.5 nm, and potassium was measured. And the amount of potassium in a sample was quantified using the calibration curve prepared beforehand.

4). Measurement of color tone change Using a spectrophotometer (model Color Meter ZE-2000, manufactured by Nippon Denshoku Industries Co., Ltd.), put the sample in a quartz cell with an optical path length of 10 mm, and b ^* value of L ^* a ^* b ^* color system Was measured. Packaged beverage immediately after production, and from the b ^* value of 55 ° C. for 7 days packaged beverage after storage, [Delta] b ^* - was determined (b ^* value after storage before storage b ^* values).

5. Evaluation method of astringency Five expert panels drank each of the packaged beverages, evaluated the astringency according to the following criteria, and then decided the final score by consultation and set it as the evaluation value.

Evaluation criteria 5: No astringency 4: Some astringency 3: Astringency 2: Astringency is slightly strong 1: Astringency is strong

6). Evaluation method over the throat Five expert panels drank each of the packaged beverages, evaluated the throat passage over the following criteria, and then determined the final score by consultation and set it as the evaluation value. The term “over the throat” as used herein was evaluated based on the level of “feeling caught in the throat” when drinking.

Evaluation criteria 5: Good over the throat (no feeling of being caught in the throat)
4: Slightly good over the throat 3: Not good 2: Slightly bad over the throat 1: Bad over the throat

Examples 1 to 11 and Comparative Examples 1 to 3
Prepared with the composition shown in Table 1, then sterilized at 85 ° C. for 2 minutes, and filled into a 200 mL PET bottle with 160 mL to prepare a packaged beverage. Table 1 shows the component analysis and evaluation results of each packaged beverage.

From Table 1, a specific amount of component (B) is added together with component (A), and the mass ratio [(B) / (A)] and pH of component (A) and component (B) are specified. By controlling within the range, it was confirmed that the color tone hardly changed even when stored for a long time, and the appearance was maintained.

Example 12
In the formulation of Example 1, ion-exchanged water was blended so as to give a 4-fold concentrated solution, the concentrated solution was sterilized at 85 ° C. for 2 minutes, and then diluted 4-fold, the carbon dioxide volume was 2.5. In this way, carbonated water cooled to 5 ° C. was blended, filled into a pressure-resistant PET bottle and wound, and then sterilized at 65 ° C. for 20 minutes to prepare a packaged beverage. When this “packed beverage” was evaluated for “color tone change” and “astringency”, the same results as in Example 1 were obtained.

Example 13
In the formulation of Example 1, a container-packed beverage was prepared in the same manner as in Example 1 except that 0.02% by mass of potassium chloride (0.0105% by mass as potassium ions) was further added. When this “packed beverage” was evaluated for “color tone change” and “astringency”, the same results as in Example 1 were obtained.

Claims

The following components (A) and (B):
(A) Isoquercitrin and its sugar adduct 0.03-0.17% by mass,
(B) At least one selected from sugar alcohol and trehalose 0.01 to 3% by mass
Containing
A packaged beverage having a mass ratio [(B) / (A)] of component (B) to component (A) of 3 to 50 and a pH of 2 to 5.
The container-packed beverage according to claim 1, wherein component (B) is at least one selected from erythritol, xylitol, trehalose and maltitol.
The container-packed drink of Claim 1 whose component (B) is at least 1 sort (s) chosen from erythritol, a xylitol, and a trehalose.
The container-packed beverage according to any one of claims 1 to 3, wherein the mass ratio of isoquercitrin sugar adduct / isoquercitrin in component (A) is 1 or more.
The container-packed beverage according to any one of claims 1 to 4, wherein a mass ratio of isoquercitrin sugar adduct / isoquercitrin in the component (A) is 1000 or less.
The container-packed beverage according to any one of claims 1 to 5, wherein the content of the component (A) is 0.01 to 0.07% by mass in terms of quercetin.