WO2013043126A8 - Method(s) for obtaining a foetal red blood cell - Google Patents

Method(s) for obtaining a foetal red blood cell Download PDF

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Publication number
WO2013043126A8
WO2013043126A8 PCT/SG2012/000345 SG2012000345W WO2013043126A8 WO 2013043126 A8 WO2013043126 A8 WO 2013043126A8 SG 2012000345 W SG2012000345 W SG 2012000345W WO 2013043126 A8 WO2013043126 A8 WO 2013043126A8
Authority
WO
WIPO (PCT)
Prior art keywords
cell
sample
blood cell
red blood
iii
Prior art date
Application number
PCT/SG2012/000345
Other languages
French (fr)
Other versions
WO2013043126A1 (en
Inventor
Jae Hyeok Choi
John Christopher Love
Sherry Sze Yee HO
Mahesh Choolani
Original Assignee
National University Of Singapore
Massachussetts Institute Of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Of Singapore, Massachussetts Institute Of Technology filed Critical National University Of Singapore
Publication of WO2013043126A1 publication Critical patent/WO2013043126A1/en
Publication of WO2013043126A8 publication Critical patent/WO2013043126A8/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

In one aspect, the invention is directed to a method of separating at least one foetal nucleated red blood cell (FNRBC) from a sample comprising: a) placing the sample on at least one support surface b) contacting the sample with at least one antibody or antigen binding fragment thereof that specifically binds to at least one white blood cell, thereby producing at least one labelled cell; and c) providing a means capable of detection and isolation of at least one cell in the sample on the support surface, the cell comprising the features of: (i) being an unlabelled cell; (ii) comprising nucleic acid; and (iii) being 5 to 25pm in size, wherein the detection of the features (i), (ii) and (iii) is done simultaneously and the cell comprising the features (i), (ii) and (iii) is the FNRBC.
PCT/SG2012/000345 2011-09-20 2012-09-20 Method(s) for obtaining a foetal red blood cell WO2013043126A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161536964P 2011-09-20 2011-09-20
US61/536,964 2011-09-20

Publications (2)

Publication Number Publication Date
WO2013043126A1 WO2013043126A1 (en) 2013-03-28
WO2013043126A8 true WO2013043126A8 (en) 2013-05-30

Family

ID=47914682

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SG2012/000345 WO2013043126A1 (en) 2011-09-20 2012-09-20 Method(s) for obtaining a foetal red blood cell

Country Status (1)

Country Link
WO (1) WO2013043126A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012141657A1 (en) * 2011-04-11 2012-10-18 National University Of Singapore A method of identifying, isolating and/or culturing foetal erythroblasts

Also Published As

Publication number Publication date
WO2013043126A1 (en) 2013-03-28

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