SG10201906124XA - Foetal nucleated red blood cell detection - Google Patents

Foetal nucleated red blood cell detection

Info

Publication number
SG10201906124XA
SG10201906124XA SG10201906124XA SG10201906124XA SG10201906124XA SG 10201906124X A SG10201906124X A SG 10201906124XA SG 10201906124X A SG10201906124X A SG 10201906124XA SG 10201906124X A SG10201906124X A SG 10201906124XA SG 10201906124X A SG10201906124X A SG 10201906124XA
Authority
SG
Singapore
Prior art keywords
sample
fnrbcs
red blood
nucleated red
separating
Prior art date
Application number
SG10201906124XA
Inventor
Aniza Puteri Mahyuddin
Sukumar Ponnusamy
Priya Kadam
Mahesh Choolani
Original Assignee
Nat Univ Singapore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Univ Singapore filed Critical Nat Univ Singapore
Publication of SG10201906124XA publication Critical patent/SG10201906124XA/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3

Abstract

FOETAL NUCLEATED RED BLOOD CELL DETECTION In one aspect, the invention is directed to methods of separating foetal nucleated red blood cells (FNRBCs) from a sample comprising contacting the sample with an agent that speCifically recognizes CD, thereby producing a mixture; maintaining the mixture under conditions in which a complex forms between the agent and the CD147 in the sample; and separating the complex from the mixture; thereby separating the FNRBCs from the sample. In other embodiments, the invention is directed to methods of detecting FNRBCs in a sample comprising contacting the sample with an antibody or antigen binding fragment thereof that specifically binds CD147, thereby producing a combination; maintaining the combination under conditions in which an immune complex forms between the antibody and FNRBCs present in the sample; and detecting whether the immune complex forms in the combination; wherein if the immune complex is detected then· FNRBCs are present in the sample. In still other aspects, the invention further comprises methods of sparating FNRBCs from anucleated RBCs, detecting prenatal disorders and/or the gender of a foetus. [FIGURE 1) 47
SG10201906124XA 2011-06-30 2012-06-28 Foetal nucleated red blood cell detection SG10201906124XA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161503236P 2011-06-30 2011-06-30

Publications (1)

Publication Number Publication Date
SG10201906124XA true SG10201906124XA (en) 2019-08-27

Family

ID=47424401

Family Applications (3)

Application Number Title Priority Date Filing Date
SG10201906124XA SG10201906124XA (en) 2011-06-30 2012-06-28 Foetal nucleated red blood cell detection
SG10201605398QA SG10201605398QA (en) 2011-06-30 2012-06-28 Foetal nucleated red blood cell detection
SG2013084405A SG194959A1 (en) 2011-06-30 2012-06-28 Foetal nucleated red blood cell detection

Family Applications After (2)

Application Number Title Priority Date Filing Date
SG10201605398QA SG10201605398QA (en) 2011-06-30 2012-06-28 Foetal nucleated red blood cell detection
SG2013084405A SG194959A1 (en) 2011-06-30 2012-06-28 Foetal nucleated red blood cell detection

Country Status (6)

Country Link
US (1) US20140141997A1 (en)
EP (1) EP2726871A4 (en)
CN (1) CN103797368A (en)
HK (1) HK1197299A1 (en)
SG (3) SG10201906124XA (en)
WO (1) WO2013002731A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106488981A (en) * 2014-05-15 2017-03-08 科尔贝恩科斯公司 The preparation of the fetal nucleated red blood (NRBCS) for diagnostic test
CN106537144A (en) * 2014-08-05 2017-03-22 富士胶片株式会社 Nucleated erythrocyte sorting method
CN105063181A (en) * 2015-07-03 2015-11-18 石莹 Method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of pregnant woman
CN111500534B (en) * 2016-07-11 2022-11-29 山东亚大药业有限公司 Kit for separating and purifying fetal nucleated red blood cells
JP6234542B1 (en) * 2016-12-27 2017-11-22 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ Method for obtaining chromosomal DNA derived from fetal cells
CN113348356A (en) * 2018-11-20 2021-09-03 文塔纳医疗系统公司 Methods and systems for preparing and analyzing cell samples for morphological features and biomarker expression
CN110904254A (en) * 2019-12-18 2020-03-24 广东龙帆生物科技有限公司 Real-time fluorescent quantitative PCR (polymerase chain reaction) detection primer and probe for Callinrelia, detection kit, detection method and application thereof
WO2024021040A1 (en) * 2022-07-29 2024-02-01 曾繁根 Cell identification method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075647A1 (en) * 1999-06-04 2000-12-14 Bioseparations, Inc. System and method for prenatal diagnostic screening
DE10035433C2 (en) * 2000-07-20 2002-07-18 Tuma Wolfgang Gentle high enrichment of fetal cells from peripheral blood and use of the same
PT2183693E (en) * 2007-07-23 2014-01-14 Univ Hong Kong Chinese Diagnosing fetal chromosomal aneuploidy using genomic sequencing
CN101139391B (en) * 2007-08-21 2012-07-25 陈志南 CD147 extracellular domain crystal structure and use thereof

Also Published As

Publication number Publication date
CN103797368A (en) 2014-05-14
WO2013002731A1 (en) 2013-01-03
US20140141997A1 (en) 2014-05-22
SG194959A1 (en) 2013-12-30
EP2726871A4 (en) 2015-03-04
SG10201605398QA (en) 2016-08-30
HK1197299A1 (en) 2015-01-09
EP2726871A1 (en) 2014-05-07

Similar Documents

Publication Publication Date Title
SG10201906124XA (en) Foetal nucleated red blood cell detection
PH12017501178A1 (en) Techniques for predicting, detecting and reducing aspecific protein interference in assays involving immunoglobulin single variable domains
NZ609363A (en) Methods for determining anti-drug antibody isotypes
MX2009013411A (en) Detection of antigens carried by erythrocytes and of anti-erythrocyte antibodies.
WO2014144129A3 (en) Biomarkers and methods for predicting preterm birth
NZ629703A (en) Methods and compositions for detecting multiple analytes with a single signal
NZ599456A (en) Assays for the detection of anti-tnf drugs and autoantibodies
WO2010002911A3 (en) Methods and materials for monitoring myeloma using quantitative mass spetrometry
JO3812B1 (en) Antibodies that bind to human programmed death ligand 1 (pd-l1)
MX2013004172A (en) Methods and kits for the detection of circulating tumor cells in pancreatic patients using polyspecific capture and cocktail detection reagents.
IN2012DN06139A (en)
MX2014003983A (en) Method for diagnosing alzheimer's disease (ad).
WO2012083020A3 (en) Antibody-linked immuno-sedimentation agent and method of isolating a target from a sample using same
WO2013049704A3 (en) Method and system of particle-phage epitope complex
RU2012148244A (en) METHOD FOR DIAGNOSTIC OF ONCOLOGICAL DISEASES AND IMMUNO-ENZYME KIT FOR ITS IMPLEMENTATION
MX2014010068A (en) Methods and systems for signal amplification of bioassays.
WO2009121024A3 (en) Methods for detecting antibodies
RU2015104737A (en) METHOD FOR DETECTING MULTIPECIFIC BINDING AGENT
WO2012139052A3 (en) Antibody biomarkers for diabetes
WO2007037910A3 (en) Specific removal of activated immune cells
MX2015004419A (en) Detecting an analyte and determining the concentration of an analyte using magnetizable beads.
WO2011003598A3 (en) Detection of antigens
WO2009023331A3 (en) Antibodies and improved test sample handling methods for use in assays for myeloperoxidase
NZ603340A (en) Yeast cell wall components and detection thereof
GB201119585D0 (en) Assay