WO2013040631A1 - Dispositif d'échantillonnage de tissu - Google Patents

Dispositif d'échantillonnage de tissu Download PDF

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Publication number
WO2013040631A1
WO2013040631A1 PCT/AU2012/001119 AU2012001119W WO2013040631A1 WO 2013040631 A1 WO2013040631 A1 WO 2013040631A1 AU 2012001119 W AU2012001119 W AU 2012001119W WO 2013040631 A1 WO2013040631 A1 WO 2013040631A1
Authority
WO
WIPO (PCT)
Prior art keywords
tip
engaging
sample
sampling
jaws
Prior art date
Application number
PCT/AU2012/001119
Other languages
English (en)
Inventor
Russell Bradford
David KUBE
Peter Martin Grewe
Original Assignee
Commonwealth Scientific And Industrial Research Organisation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2011903861A external-priority patent/AU2011903861A0/en
Application filed by Commonwealth Scientific And Industrial Research Organisation filed Critical Commonwealth Scientific And Industrial Research Organisation
Publication of WO2013040631A1 publication Critical patent/WO2013040631A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A61B10/0233—Pointed or sharp biopsy instruments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A61B2010/0208—Biopsy devices with actuators, e.g. with triggered spring mechanisms

Definitions

  • This invention relates to a tissue sampling device, and in particular to a device adapted for harvesting and manipulating tissue samples taken from biological materials including samples from live biological materials.
  • tissue sampling tools and devices are not usually purpose built and do not provide for the rapid and reliable extraction and harvesting of tissue samples.
  • such devices do not provide for single handed or "hands free” manipulation and processing of such samples and often require multiple handling steps which introduce a high risk of contamination between samples and sample degradation.
  • One object of the invention is to provide an improved tissue sampling device.
  • the invention provides a sampling device adapted to remove a sample of biological material from a target and dispense said sample to a testing station, said device comprising an applicator body adapted for manipulating said device and a sampling tip adapted to harvest said sample and co-operate with said body wherein said body includes a tip engaging/releasing means adapted for hands free engagement and release of said tip, said tip engaging/releasing means including a tip engaging catch and a tip opening rim and wherein said tip includes a sample piercing point at a first end, engaging/releasing jaws at a second end and an intermediate sampling barb such that said tip is engaged by the partial introduction of said engaging/releasing means between said tip jaws causing said engaging catch to positively engage said jaws and prevent premature release of said tip during removal of said sample and wherein said tip is released from said positive engagement by the full introduction of said engaging/releasing means between said tip jaws causing said tip opening rim to open and disengage said jaws.
  • the tip engaging/releasing means may include a tip ejection plunger coaxially fitted for telescopic movement between a first retracted position and a second extended position to actively eject said released tip.
  • the tip engaging catch is preferably a frustroconical cone separated from the tip opening rim by a locking collar of reduced diameter.
  • the tip engaging catch and tip opening rim preferably move telescopically relative to each other.
  • the tip jaws preferably include an internal locking lip and opening chamfer defining the jaw lock bolt which is configured to snugly and positively engage said locking collar.
  • the tip jaws are preferably separated by elongate jaw slots.
  • the tip ejection plunger preferably includes an extension shaft passing through the length of the body exiting at the near end thereof and terminating in an operating button.
  • the body is preferably generally cylindrical with a finger stop to assist manual operation of said device.
  • the device may further include a tip abutting collar associated with the tip opening rim.
  • the collar may communicate with the operating tip via a hollow collar drive tube passing through the inside of the body.
  • the plunger extension shaft and collar drive tube may operate telescopically with the plunger extension shaft fitting within the hollow collar drive tube. In this manner, both the plunger and collar drive tube can be operated simultaneously by the single operating button.
  • the tip opening rim preferably moves telescopically relative to the tip engaging catch with the tip engaging/releasing means telescopically fitted to the body of the device for movement relative thereto.
  • the invention provides a tissue sampling and analysing system comprising a tissue sampling device as previously described, a sample storage means to store one or a plurality of said samples, a sample extraction means to extract a sample as required; and an analysis means to analyse said sample.
  • the invention provides a sampling tip for use in said sampling device, preferably a biopsy sampling tip, comprising a generally conically shaped first end having a piercing point at the apex of the said cone and a barb at the base of said cone wherein said tip includes two or more resilient engaging/releasing jaws at a second end thereof said jaws being separated by an elongate longitudinal slot and being adapted to move between a close engaging position and an open releasing position against the bias of said resilience.
  • the invention provides an applicator body for mounting the sampling tip, preferably a biopsy sampling tip, said body comprising a tip engaging/releasing means adapted for hands free engagement and release of said tip, said tip engaging/releasing means including a tip engaging catch and a tip opening rim adapted for two stage operation whereby the tip is engaged by the partial introduction of said engaging/releasing means and released by the full introduction of said engaging/releasing means.
  • biopsy sampling tip and applicator body cooperate to provide the device as previously described.
  • the invention provides a process for obtaining and manipulating a biological sample from an organism comprising the use of a device as previously described to obtain said sample including the steps of:
  • the analysis container is manually sealed for sterile storage until sample analysis is performed.
  • the sample directly undergoes analysis.
  • the biological sample is a biopsy sample.
  • the organism is an aquatic organism.
  • the invention provides use of a sampling device as previously described, for gene tagging of an organism.
  • the organism is an aquatic organism.
  • Figure 1 shows a side view of the device body and sampling tip prior to tip engagement.
  • Figure 2 shows the tip engagement by co-operation between the tip engaging/releasing means and the tip jaws.
  • Figure 3 shows the first stage of tip ejection with the tip jaws opened for tip release.
  • Figure 4 shows the ejection of the tip by the second stage of tip ejection with the telescopic extension of the tip plunger.
  • FIG. 5 shows detail of the tip.
  • Figure 6 shows an exploded view of the sampling device applicator.
  • sampling device adapted for use as a hand held and manually operated tool including an applicator body and a sampling tip with the applicator and sampling tip adapted to work in concert and the applicator specifically configured for hands free locking engagement of the sampling tip, and hands free release and ejection of the sampling tip in a two stage action.
  • the hands free engagement/release of the sampling tip allows single handed use of the device freeing up the user' s second hand for manipulation of the organism being sampled.
  • the ability to handle the organism with one hand and manipulate the sampling device with the other hand provides many advantages including the ability to readily control and manage the sample and simultaneously activate the sampling device to obtain the sample from the living and often moving organism.
  • Biopsies taken with the use of the device of the invention substantially eliminate the need for sub-sampling of biopsies prior to DNA/RNA extraction and accordingly provide greatly enhanced efficiencies in gene tagging processes.
  • Sampling device of the invention is particularly adapted for use in taking biopsy samples from fish where the one handed operation of the device allows confident and robust manipulation of the sampling tip whilst the free hand of the user can hold the fish (and subsequently the analysis container) and thereby exercise full control during the sampling procedure.
  • the device of the invention is particularly adapted as previously described it is capable of application and use in a wide range of sampling techniques and situations which would benefit from single handed operation of the device to obtain the sample and the hands free engagement and release of the tip thereby minimising any potential for contamination.
  • the device of the invention is shown in a particular and preferred configuration as a hand held tool comprising the applicator 1 being formed as a generally elongate and cylindrical body 2, dimensioned for comfortable grasping by the operators fingers in the manner of a pipette or like device.
  • the body is provided with a tip engaging/releasing means 13 at the remote end thereof.
  • the tip engaging/releasing means is adapted for two stage operation by way of the relative telescopic and coaxial movement back and forth of its component parts, relative to the body 2.
  • the tip engaging/releasing means is made up of a tip abutting collar 16 having an integral tip opening rim 17, and a tip engaging catch 14 having an integral locking collar 21, adapted to telescopically co-operate with the tip abutting collar by sliding back and forth with the frustroconical shaped tip engaging catch 14 abutting and retreating from the tip opening rim 17 so as to open and close the tip locking collar region 21.
  • the diameter of the frustroconical base preferably corresponds with the diameter of the tip opening rim so as to provide a smooth release of the tip as the tip locking collar region is closed.
  • the telescopic and coaxial operation of the tip engaging/releasing means is effected by the provision of an internally mounted shaft 6 and tube 7 co-axially within the interior of the body 2, passing through the body and exiting at the near end 3, terminating in an operating button 5 adapted for operation by the thumb of the user.
  • the near end 3 of the body 2 may be provided with a projecting finger stop 4, such that application of pressure to the button 5 by the operator's thumb causes the plunger extension shaft 6 and associated collar drive tube 7 to move coaxially down the interior of the body 2 to act on the tip engaging/releasing means whereby any tendency for the body 2 to slide through the operator's fingers is resisted by the finger stop 4.
  • the operator can confidently grasp the body 2 of the applicator 1 and effect a positive and highly controlled activation of the device by thumb operated control of the button 5.
  • the tip engaging/releasing means 13 provides for the one handed engagement of a tip 11 and two stage release and ejection of the tip once the sample has been obtained.
  • the tips 11 may be provided in an array on a sterilised and secure tray including a plurality of sampling tips whereby the device of the invention as shown in Figure 2, can be caused to engage one of the sampling tips 11 by applying the tip engaging/releasing means 13 to one of the tips 11, so as to effect engagement.
  • the engagement is effected by the cooperation of the generally frustroconical shaped tip engaging catch 14 which is positioned at the remote end of the tip engaging/releasing means 13.
  • the tip engaging catch 14 is adapted to cooperate with engagement/releasing jaws 18 provided at the base of the tip 11.
  • the jaws 18 are formed as a portion of the cylindrical base of the tip and separated by elongate slots 23, thereby allowing the jaws to expand to engage the tip engaging catch 14 and elastically return to lockably engage the jaws 18 with the tip engaging catch 14.
  • the jaws 18 are provided with an internal lip 20 being opposed by an exterior chamfer 19 thereby defining an internal collar-shaped lock bolt 22.
  • the resting position of the tip engaging/releasing means 13 has the tip engaging catch 14 distanced from the tip opening rim 17 so as to expose a portion locking collar 21.
  • the exposed or open locking collar 21 receives the lock bolt portion 22 of the jaws 18 and effects the secure locking of the tip to the applicator.
  • the tip 11 is then securely and positively locked to the applicator 1.
  • the body 2 can then be withdrawn from the tray or other storage device holding the tips.
  • the device of the invention is now ready for use for sampling and the tip can be confidently manipulated due to the positive engagement and locking to the body 2.
  • the tip is provided with a sampling barb 12, providing a means for removing a tissue sample from the target organism which can be a fish, animal, plant or any other material requiring a fairly robust insertion of the tip to penetrate fish scales, plant tissue, cuticles or any other form of protective coating where the tip can be applied with sufficient force to penetrate the same and by active withdrawal, the reverse angle of the sampling barb 12 extracts a generally torus shaped sample from the target.
  • Modifications of the tip may include alternative sample shapes including oval.
  • the ability of the device of the invention to securely lock the tip allows the robust application of the necessary force required to confidently take a uniform sample in the manner previously described.
  • the device of the invention is now used to release the tip and sample in a manner avoiding any contact or handling of the tip to separate the charged tip from the applicator 1.
  • This automated and hands free action is accomplished by the two stage action of the tip engaging/releasing means 13 whereby partial depression of the button 5, causes a first telescopic reaction with the collar drive tube 7 moving down the interior of the body 2.
  • the remote end of the collar drive tube 7 is provided with a collar drive pin 27.
  • the collar drive pin 27 co-operates with the collar pin receiving aperture 26 formed in the collar 16 thereby causing the collar 16 and integral tip opening rim 17 to draw away from the body 2.
  • the collar and integral tip 17 move telescopically along the locking collar 21 to simultaneously close the locking collar and open the tip jaws 18 as the tip opening rim 17 bears upon the chamfer 19 to draw the jaws open.
  • the tip opening rim 17 has a diameter greater than the lock bolt opening so as to cause the jaws to be drawn apart thereby releasing the lock bolt 22 from within the confines of the locking collar 21 and the tip engaging catch 14.
  • the combined action of the two parts of the tip engaging/releasing means releases the tip from the tip engaging means.
  • the tip opening rim may be provided with an enlarged collar 16 acting as a tip abutting collar limiting the penetration of the tip opening rim and preventing premature ejection of the released tip.
  • the tip jaws 18 have been opened, thereby allowing the tip to be released from the tip engaging/releasing means, but without active ejection of the tip.
  • the device in order to actively eject the tip from the tip engaging/releasing means, the device includes a plunger extension shaft 6 terminating in a plunger 15.
  • the plunger extension shaft 6 is dimensioned to fit within the collar drive tube 7 with the plunger portion 15 dimensioned to pass through the tip engaging catch 14 and integral locking collar 21.
  • the collar drive tube 7, plunger extension shaft 6 and plunger 15 all fit together co-axially for telescopic movement back and forth from a first passive position shown in Figure 3, to a second active position shown in Figure 4.
  • the second stage action of the device is effected by further depression of the button 5 causing the plunger to actively bear down upon the released tip which is then ejected from the tip engaging/releasing means and removed from the applicator 1 of a device.
  • the remote end of the body may be provided with a collar guide 8.
  • the collar guide 8 is adapted to receive the remote end of the collar drive tube internally and the collar externally so as to control the telescopic movement of the respective parts and ensure accurate co-axial movement therein between.
  • a plunger spring 9 and collar spring 10 are provided within the body 2 so as to ensure a smooth continuous movement of the plunger extension shaft and the collar drive tube during depression of the button 5.
  • the collar drive pin 27 communicates with the collar pin receiving aperture 26 of the collar 16 via the elongate collar pin slot 25. The elongate length of the collar pin slot provides sufficient latitude for the collar drive pin to move the collar 16 and integral tip opening rim over the two stage action.
  • the tip with the sample attached can thereby be moved from the device without any manual handling and can be placed into a storage device or directly into a testing station for analysis.
  • the tip is formed of suitable resilient materials providing the flexibility required to move the jaws to the open position, whilst retaining sufficient grip to keep the tip firmly locked to the catch during use.
  • the tip may be provided with a circumferential tip stop 24 positioned around the rim of the tip toward the centre thereof.
  • the tip stop 24 is adapted to assist in the confident placement of the tip into an analysis container or tube (e.g. eppendorf tube) or other piece of analytical equipment.
  • the tip stop 24 also ensures that the tip is not plunged too deeply into the target tissue and, thereby minimising any risk of the tissue sample contaminating the body 2 of the applicator 1, thereby minimising any chance of cross contamination from subsequent sampling tips.
  • the tip includes a conically shaped nose 28 terminating at the remote end in a sharp pointed end 29.
  • the sharp pointed end is adapted to pierce the flesh or sample to be taken so as to ease penetration of the tip.
  • the sampling barb is positioned at the rear end of the conical shaped nose and directed in an opposed manner to the pointed nose end 29. In this way the tip is adapted to penetrate the sample organism with the conical shaped nose opening up the wound as the tip is pushed into the sample organism. Once the tip penetrates to the barb region 12, the flesh of the sample tends to close in over the barb such that retraction of the tip allows the barb to remove a torus shaped sample. The full removal of the tip then allows the wound to draw closed.
  • the configuration of the sampling tip minimises damage to the target and maximises the ability of the target organism to repair the wound.
  • the particular action of the tip with the barb removing the sample during retraction places a high degree of stress on the tip which must be positively engaged to the applicator to prevent premature release prior to full retraction from the target being sampled.
  • the insertion of the tip acts as a self cleansing step which further reduces cross-contamination from surface biological residue that may be present from other fish.
  • the tip stop 24 also provides a region of the tip which may be adapted to receive bar code identification of the like with the option of recording sample date including date, time, size and ID of the organism, GPS location and the like. Moreover, the tip stop 24, ensures a neat, precise and secure fit for the sampling tip into eppendorf or similar microfuge tubes, eppendorf deep well plates and the like, therefore ensuring that the tip remains in a relatively vertical aspect after delivery to the storage tube in order to improve contact between the tissue sample held thereon and the DNA stabilising and/or digestion liquids used in the subsequent analysis steps.
  • the barb of the tip may be customised to remove a specific sample size as required.
  • the tip barb is configured to remove 15mg of tissue uniformly at each sample. The invention therefore provides the advantage of rendering highly uniform samples which streamlines the extraction of DNA and avoids the requirements to normalise the DNA obtained over a range of samples.
  • FTA card One advantage of FTA card is that the DNA is extracted at the time of sampling and is rendered stable for long-term storage without the need for special handling (i.e. it can be kept at room temperature for at least 14 years). FTA card eliminates the need for an extraction phase in subsequent analyses; however, the card is generally sub-sampled prior to further analyses.
  • the mean weight of tissue collected from 11 samples using the device of the invention was 14.3 mg ( ⁇ 1.19 mg).
  • the samples were subjected to an extraction step and then analysed for DNA.
  • the amount of DNA extracted per sample using the device of the invention was approximately 4.32 ug total genomic DNA.
  • a second extraction was performed, to look at the possibility of using remaining tissue as an archive, yielding approximately 2.1 ug total genomic DNA.
  • Both the muscle biopsy and the FTA card require some form of sub-sampling prior to further analyses which is avoided by the device of the invention.
  • FTA card technology allows samples to be stored at room temperature.
  • both muscle biopsies and samples collected using the device of the invention require some form of post collection handling to ensure the DNA remains stable - for example, freezing or placing into special DNA stabilising liquids.
  • Trials of the device of the invention, FTA card, and fin clip techniques were carried out on live southern bluefin tuna.
  • the aims of the field trial were to determine the extent of damage caused by the various sampling techniques, and the degree to which the damage was visible after a period of non-disturbance.
  • Each fish was removed from the water, total length recorded, conventional dart tag applied, tissue samples were then collected using the device of the invention, FTA card and from a small fin clip from the caudal fin.
  • the device of the invention created a puncture wound of approximately 5 mm in diameter. Some of the wounds produced an amount of blood, while others did not (this is consistent with observations of wounds created during conventional dart tag application).
  • FTA card dabbed onto the slime coating tended to remove the slime coating entirely at the sample site. However, the slime coating was expected to be rapidly repaired once the fish was back in the water.
  • the fin clip removed a visible portion of the tip of the caudal fin.
  • the fish were returned to the water after sampling and thereafter handled according to commercial grow-out protocols. All samples from the device of the invention were processed on return to the laboratory.
  • the amount of DNA extracted per sample using the device of the invention was approximately 6.5 ug total genomic DNA. Fin clips were typically sub-sampled before further analyses; the amount of DNA extracted per fin clip was approximately 16.0 ug total genomic DNA.
  • the amount of DNA extracted per FTA card was approximately 2.4 ug total genomic DNA.
  • FTA card technology produced some difficulties under at-sea conditions. Subsequent DNA analysis of the FTA cards indicated that there was no cross- contamination between fish. However, there were issues with using the FTA cards on the vessel that would reduce their suitability under at-sea conditions.
  • the device Whilst the invention has been described with reference to manual use of the device of the invention, the device is equally applicable to high levels of automation where the applicator 1 can be reconfigured for use with a robotically operated tools and the button 5 is substituted with suitable automation rams and the like.
  • the invention provides for the first time a dedicated device for taking tissue samples from a range of living organisms, the device being particularly adapted to combine an applicator and a range of dedicated sampling tips which are used in cooperation, allowing the tips to be sterilised and accessed via the device of the invention without the requirement for any manual handling of the tip and subsequent engagement of the tip allowing robust and confident manipulation of the tip once fitted to the device and a two stage action where, the tip is adapted for release and ejection from the applicator as a hands free operation, allowing the charged sampling tip to be delivered, storage and/or analysis.
  • the sampling device of the invention is particularly adapted for use with dedicated sampling tips with the tips including opposed sample piercing points and sampling barbs such that minimal wounding occurs to the target organism during the taking of the sample with the sample piercing point penetrating and opening up the flesh of the target organism such that further penetration of the tip to the intermediate barbs causes the flesh of the target organism to close around the barbs. Retraction of the tip at that point allows the barb to secure a small torus shaped sample with the wound closing back once the tip is withdrawn.
  • the manner in which the sample is taken by the tip applies substantial withdrawing stresses to the tip as it is drawn out from the target organism with the barb removing the torus shaped sample and the invention provides for the first time a dedicated device adapted for such rigorous use of the sampling tip whilst also allowing for the hands-free manipulation of the tip once the sample has been secured.
  • the device of the invention is highly intuitive to use and can be operated by persons with minimal training to reliably obtain tissue samples with minimal risk of contamination.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Physics & Mathematics (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention porte sur un dispositif d'échantillonnage apte à retirer un échantillon de matière biologique d'une cible et distribuer ledit échantillon à une station d'essai, ledit dispositif comprenant un corps d'applicateur (1) adapté à manipuler ledit dispositif et une pointe d'échantillonnage (11) adaptée à récolter ledit échantillon et coopérer avec ledit corps, ledit corps comprenant un moyen de prise/libération de pointe (13) adapté à une prise et une libération sans mains de ladite pointe, ledit moyen de prise/libération de pointe comprenant un loquet de prise de pointe (14) et un rebord d'ouverture de pointe (17), et ladite pointe comprenant un point de perçage d'échantillon (29) à une extrémité, des mâchoires de prise/libération (18) à une seconde extrémité et une dent d'échantillonnage intermédiaire (12) de telle sorte que ladite pointe est en prise par l'introduction partielle dudit moyen de prise/libération entre lesdites mâchoires de pointe amenant ledit loquet de prise à venir en prise de manière positive avec lesdites mâchoires, et ladite pointe étant libérée de la prise positive par l'introduction totale dudit moyen de prise/libération entre lesdites mâchoires de pointe amenant ledit rebord d'ouverture de pointe à s'ouvrir et se désengager desdites mâchoires.
PCT/AU2012/001119 2011-09-20 2012-09-19 Dispositif d'échantillonnage de tissu WO2013040631A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2011903861 2011-09-20
AU2011903861A AU2011903861A0 (en) 2011-09-20 Tissue sampling device

Publications (1)

Publication Number Publication Date
WO2013040631A1 true WO2013040631A1 (fr) 2013-03-28

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108489753A (zh) * 2018-03-27 2018-09-04 俞琳琳 一种分体式滚柱生物检材提取器
WO2019235340A1 (fr) * 2018-06-05 2019-12-12 国立研究開発法人海洋研究開発機構 Dispositif de biopsie, appareil d'injection permettant d'injecter ledit dispositif de biopsie et appareil de biopsie équipé de ce dernier
CN112146919A (zh) * 2020-08-31 2020-12-29 王艺烨 一种用于污染土壤再生的采样设备
CN117064456A (zh) * 2023-10-17 2023-11-17 江西省水产科学研究所(江西省鄱阳湖渔业研究中心、江西省渔业资源生态环境监测中心) 一种鲫鱼类免疫组织自动取样装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741177A (en) * 1994-06-28 1998-04-21 Meat Research Corporation Tissue sampling and analysis
US20040167429A1 (en) * 2003-02-20 2004-08-26 Roshdieh Babak B. Cutaneous biopsy device with handle and disposable tips
US20110071432A1 (en) * 2009-09-24 2011-03-24 Carrillo Jr Oscar R Fine Needle Aspiration Device with Distal Anchor
WO2011071555A1 (fr) * 2009-12-10 2011-06-16 Cermed Corporation Dispositif de prélèvement et de transfert de cellules, et procédés d'utilisation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741177A (en) * 1994-06-28 1998-04-21 Meat Research Corporation Tissue sampling and analysis
US20040167429A1 (en) * 2003-02-20 2004-08-26 Roshdieh Babak B. Cutaneous biopsy device with handle and disposable tips
US20110071432A1 (en) * 2009-09-24 2011-03-24 Carrillo Jr Oscar R Fine Needle Aspiration Device with Distal Anchor
WO2011071555A1 (fr) * 2009-12-10 2011-06-16 Cermed Corporation Dispositif de prélèvement et de transfert de cellules, et procédés d'utilisation

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108489753A (zh) * 2018-03-27 2018-09-04 俞琳琳 一种分体式滚柱生物检材提取器
WO2019235340A1 (fr) * 2018-06-05 2019-12-12 国立研究開発法人海洋研究開発機構 Dispositif de biopsie, appareil d'injection permettant d'injecter ledit dispositif de biopsie et appareil de biopsie équipé de ce dernier
CN112146919A (zh) * 2020-08-31 2020-12-29 王艺烨 一种用于污染土壤再生的采样设备
CN117064456A (zh) * 2023-10-17 2023-11-17 江西省水产科学研究所(江西省鄱阳湖渔业研究中心、江西省渔业资源生态环境监测中心) 一种鲫鱼类免疫组织自动取样装置
CN117064456B (zh) * 2023-10-17 2024-02-02 江西省水产科学研究所(江西省鄱阳湖渔业研究中心、江西省渔业资源生态环境监测中心) 一种鲫鱼类免疫组织自动取样装置

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