WO2013027053A3 - Method for enhancement of enzyme activity - Google Patents

Method for enhancement of enzyme activity Download PDF

Info

Publication number
WO2013027053A3
WO2013027053A3 PCT/GB2012/052052 GB2012052052W WO2013027053A3 WO 2013027053 A3 WO2013027053 A3 WO 2013027053A3 GB 2012052052 W GB2012052052 W GB 2012052052W WO 2013027053 A3 WO2013027053 A3 WO 2013027053A3
Authority
WO
WIPO (PCT)
Prior art keywords
enhancement
activity
enzyme activity
microwave energy
enhance
Prior art date
Application number
PCT/GB2012/052052
Other languages
French (fr)
Other versions
WO2013027053A2 (en
Inventor
Ahmed Ibrahim Al-Shamma'a
David Arthur Phipps
Alexander James STAVRINIDES
Original Assignee
Liverpool John Moores University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Liverpool John Moores University filed Critical Liverpool John Moores University
Priority to GB1404299.8A priority Critical patent/GB2507711B/en
Publication of WO2013027053A2 publication Critical patent/WO2013027053A2/en
Publication of WO2013027053A3 publication Critical patent/WO2013027053A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to the use of microwave energy to enhance the activity of enzymatic reactions, such as enhancing the activity of enzymes involved in the degradation of cellulose to glucose and intermediates thereto. The invention further relates to the use of microwave energy to enhance the production of compounds by microbes, specifically where microbial growth is fuelled by an energy source, such as a biomass whose degradation is enhanced by microwave energy. The invention further relates to a microwave reactor vessel suitable for conducting such enhancement of enzyme activity and such fuelling of microbial growth.
PCT/GB2012/052052 2011-08-22 2012-08-22 Method for enhancement of enzyme activity WO2013027053A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1404299.8A GB2507711B (en) 2011-08-22 2012-08-22 Method for enhancement of enzyme activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1114425.0 2011-08-22
GB201114425A GB201114425D0 (en) 2011-08-22 2011-08-22 Method for enhancement of enzyme activity

Publications (2)

Publication Number Publication Date
WO2013027053A2 WO2013027053A2 (en) 2013-02-28
WO2013027053A3 true WO2013027053A3 (en) 2013-05-10

Family

ID=44800649

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2012/052052 WO2013027053A2 (en) 2011-08-22 2012-08-22 Method for enhancement of enzyme activity

Country Status (2)

Country Link
GB (3) GB201114425D0 (en)
WO (1) WO2013027053A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109206760B (en) * 2018-09-07 2021-02-23 宿州学院 Preparation method of natural plant fiber-rubber composite material

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017035253A1 (en) 2015-08-24 2017-03-02 The Regents Of The University Of California Methods and compositions for protein purification and enzyme reaction
IT202000019000A1 (en) * 2020-08-03 2022-02-03 Ingenio S R L METHOD AND DEVICE FOR THE REGULATION OF CHEMICAL-PHYSICAL REACTIONS IN A MATERIAL

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2353280A (en) * 1999-05-03 2001-02-21 Procter & Gamble Methods for activating enzymes
WO2008064429A1 (en) * 2006-12-01 2008-06-05 Cellencor, Inc Treatment of cellulosic material for ethanol production

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH697035A5 (en) 1999-05-04 2008-03-31 Marcel Roell Bioreactor.
US6544788B2 (en) 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2353280A (en) * 1999-05-03 2001-02-21 Procter & Gamble Methods for activating enzymes
WO2008064429A1 (en) * 2006-12-01 2008-06-05 Cellencor, Inc Treatment of cellulosic material for ethanol production

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
A AL-SHAMAA ET AL: "Novel Techniques using FEM for material production and processing", PROCEEDINGS OF THE 27TH INTERNATIONAL FREE ELECTRON LASER CONFERENCE, 21 August 2005 (2005-08-21), pages 339 - 342, XP055041594, Retrieved from the Internet <URL:http://www.slac.stanford.edu/econf/C0508213/PAPERS/TUPP053.PDF> [retrieved on 20121019] *
DHOLIYA KRUNAL ET AL: "Effect of low power microwave on microbial growth, enzyme activity, and aflatoxin production", RESEARCH IN BIOTECHNOLOGY, 1 January 2012 (2012-01-01), pages 28 - 34, XP055041613, Retrieved from the Internet <URL:http://www.researchinbiotechnology.com/getarticle.php?volid=3&issid=4&artid=4> [retrieved on 20121019] *
DOUGLAS D. YOUNG ET AL: "Microwave Activation of Enzymatic Catalysis", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130, no. 31, 1 August 2008 (2008-08-01), pages 10048 - 10049, XP055041324, ISSN: 0002-7863, DOI: 10.1021/ja802404g *
GALVIN M J ET AL: "Influence of 2.45 GHz microwave radiation on enzyme activity", RADIATION AND ENVIRONMENTAL BIOPHYSICS, SPRINGER VERLAG, DE, vol. 19, no. 2, 1 January 1981 (1981-01-01), pages 149 - 156, XP009163919, ISSN: 0301-634X *
PRAMANIK B N ET AL: "Microwave-enhanced enzyme reaction for protein mapping by mass spectrometry: A new approach to protein digestion in minutes", PROTEIN SCIENCE, CAMBRIDGE UNIVERSITY PRESS, vol. 11, no. 11, 1 November 2002 (2002-11-01), pages 2676 - 2687, XP002457998, ISSN: 0961-8368, DOI: 10.1110/PS.0213702 *
SUN W ET AL: "Microwave-assisted protein preparation and enzymatic digestion in proteomics", MOLECULAR & CELLULAR PROTEOMICS, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US, vol. 5, no. 4, 1 April 2006 (2006-04-01), pages 769 - 776, XP002457999, ISSN: 1535-9476, DOI: 10.1074/MCP.T500022-MCP200 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109206760B (en) * 2018-09-07 2021-02-23 宿州学院 Preparation method of natural plant fiber-rubber composite material

Also Published As

Publication number Publication date
GB201609333D0 (en) 2016-07-13
GB201404299D0 (en) 2014-04-23
GB2535920B (en) 2017-04-12
GB2507711B (en) 2017-04-12
WO2013027053A2 (en) 2013-02-28
GB2535920A (en) 2016-08-31
GB2507711A (en) 2014-05-07
GB201114425D0 (en) 2011-10-05

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