WO2013024906A2 - Acidophilus milk made from mammal bone and preparation method thereof - Google Patents

Acidophilus milk made from mammal bone and preparation method thereof Download PDF

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Publication number
WO2013024906A2
WO2013024906A2 PCT/KP2012/000029 KP2012000029W WO2013024906A2 WO 2013024906 A2 WO2013024906 A2 WO 2013024906A2 KP 2012000029 W KP2012000029 W KP 2012000029W WO 2013024906 A2 WO2013024906 A2 WO 2013024906A2
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WO
WIPO (PCT)
Prior art keywords
bone
temperature
acidophilus milk
pressure
milk
Prior art date
Application number
PCT/KP2012/000029
Other languages
French (fr)
Other versions
WO2013024906A3 (en
Inventor
Chong Mi KIM
Kwang Chol Rl
Ki Won CHON
Hye Yong SON
Original Assignee
Kim Chong Mi
Kwang Chol Rl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kim Chong Mi, Kwang Chol Rl filed Critical Kim Chong Mi
Publication of WO2013024906A2 publication Critical patent/WO2013024906A2/en
Publication of WO2013024906A3 publication Critical patent/WO2013024906A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1238—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1315—Non-milk proteins or fats; Seeds, pulses, cereals or soja; Fatty acids, phospholipids, mono- or diglycerides or derivatives therefrom; Egg products

Definitions

  • Acidophilus milk made from mammal bone and preparation method thereof
  • the present invention relates to an acidophilus milk made from animal bone, in particular mammal bone and preparation method thereof.
  • the technology was developed to prepare the beverages such as yoghurt and condensed milk by using the bone powder or protein liquid produced from the animal bone.
  • CN02107624 "Yoghurt containing bone protein liquid and preparation method” disclosed the method that prepares the bone yoghurt from the fresh milk through adding the bone protein liquid extracted from the fresh bone of animal to the said fresh milk, nano-class superemulsifying, sterilizing and fermenting.
  • CN200710016156,9 “Condensed milk made from bones” disclosed the method that produces the condensed milk containing bone powder. In the method, the condensed milk is made by mixing fine bone powder with milk.
  • the object of the present invention is to extract all nutrients from the bone and marrow and prepare acidophilus milk rich in calcium, phosphorus and protein that are good for human health by liquefying the animal bone with the high pressure and temperature.
  • One aspect of the invention relates to acidophilus milk prepared from mammal bone.
  • acidophilus milk is the lactic fermented milk made by the lactobacillus acidophilus, so that it can strongly inhibit the intestinal bacteria and also promote the digestion and absorption.
  • Another aspect of the invention relates to the method of preparing acidophilus milk from mammal bone.
  • This method comprises the following steps: cleaning and cutting of the bone, liquefying, mixing, sterilizing and cooling, and lactic fermenting. '
  • the method for preparing acidophilus milk from the mammal bone comprises the following steps: cleaning and cutting of the bone, liquefying, mixing, sterilizing and cooling, and lactic fermenting.
  • cleaning and cutting step the bones are cleaned and cut into the 30mm-length pieces.
  • the upper part of the tank has a high-pressure valve that is connected with the saturation steam boiler.
  • the high-pressure valve is to deliver the saturated steam into the tank.
  • the lower part of the tank has an exhaust valve through that the bone juice can be drawn out.
  • the saturation steam boiler is powered by electricity.
  • the heat-resistant rubber packing is fitted into the lids covering the holes in the top and bottom surface of the tank, so that the high-pressure tank can be covered tightly.
  • the high-pressure valve, the exhaust valve and the bottom lid are firstly closed.
  • the bone pieces are charged with the stainless steel rod through the top hole.
  • the top lid is closed and the high-pressure tank is covered tightly.
  • the electric power of the saturation steam boiler is turned on.
  • the high- pressure valve is opened.
  • the temperatures of the high-pressure tank and the saturation steam boiler reach 222.9°C and their pressures reach 2.5MPa, i.e. the temperature and the pressure of the high-pressure tank and the saturation steam boiler are in equilibrium state
  • the electric power of the saturation steam boiler is turned off.
  • the high-pressure valve is closed and the exhaust valve is opened to let the liquefied bone juice come out.
  • the degreased milk powder is mixed with the bone juice. At this time, the milk powder is added in the amount of 10% of the bone juice.
  • the mixture is boiled at the temperature of 100°C for 20 minutes, while being stirred. Then the mixture is rapidly cooled to the temperature of40 ⁇ 45°C.
  • a spore culture fluid in the amount of 1.25% of the mixture is added to the mixture.
  • the spore culture fluid is made by mixing Lactobacillus Bulgaricus and Streptococcus in the ratio of 1 :4. And then the mixture is fermented at the temperature of 40 ⁇ 43°C for 4-5 hours.

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)

Abstract

The present invention relates to an acidophilus milk made from animal bone, in particular mammal bone and preparation method thereof. Its preparation method comprises the following steps: cleaning the bone and cutting them into the 30mm-length pieces; liquefying the cut bones at the temperature of 222.9°C and the pressure of 2.5MPa; mixing degreased milk powder with the liquefied bone juice; sterilizing and cooling the mixture; and lactic acid fermenting.

Description

Acidophilus milk made from mammal bone and preparation method thereof
Field of the invention
The present invention relates to an acidophilus milk made from animal bone, in particular mammal bone and preparation method thereof.
Background of the invention
In the past, many efforts were made to prepare the nutritious foods from animal bone, for the animal bone contains a large amount of protein, essential amino acid, calcium, phosphor, etc.
In order to enhance absorptivity of calcium which is good for human health, the methods that produce calcium powder through steps of soaking the bones of the animals in slaked lime solution, taking out, degreasing, drying and pulverizing, have been discovered.
In addition, the technology was developed to prepare the beverages such as yoghurt and condensed milk by using the bone powder or protein liquid produced from the animal bone.
CN02107624 "Yoghurt containing bone protein liquid and preparation method" disclosed the method that prepares the bone yoghurt from the fresh milk through adding the bone protein liquid extracted from the fresh bone of animal to the said fresh milk, nano-class superemulsifying, sterilizing and fermenting. CN200710016156,9 "Condensed milk made from bones" disclosed the method that produces the condensed milk containing bone powder. In the method, the condensed milk is made by mixing fine bone powder with milk.
The object of the present invention is to extract all nutrients from the bone and marrow and prepare acidophilus milk rich in calcium, phosphorus and protein that are good for human health by liquefying the animal bone with the high pressure and temperature.
Summary of the invention
One aspect of the invention relates to acidophilus milk prepared from mammal bone.
In general, acidophilus milk is the lactic fermented milk made by the lactobacillus acidophilus, so that it can strongly inhibit the intestinal bacteria and also promote the digestion and absorption.
Another aspect of the invention relates to the method of preparing acidophilus milk from mammal bone.
This method comprises the following steps: cleaning and cutting of the bone, liquefying, mixing, sterilizing and cooling, and lactic fermenting. '
Detailed description of the invention
The method for preparing acidophilus milk from the mammal bone comprises the following steps: cleaning and cutting of the bone, liquefying, mixing, sterilizing and cooling, and lactic fermenting. In cleaning and cutting step, the bones are cleaned and cut into the 30mm-length pieces.
These pieces are liquefied in the high-pressure tank using the steam from saturation steam boiler. At the top surface of the high-pressure tank, there is a hole(hereinafter a top hole) to supply the said bone pieces and at the bottom surface, a hole(hereinafter a bottom hole) to take the residue out. The upper part of the tank has a high-pressure valve that is connected with the saturation steam boiler. The high-pressure valve is to deliver the saturated steam into the tank. The lower part of the tank has an exhaust valve through that the bone juice can be drawn out. The saturation steam boiler is powered by electricity. The heat-resistant rubber packing is fitted into the lids covering the holes in the top and bottom surface of the tank, so that the high-pressure tank can be covered tightly.
In liquefying step, the high-pressure valve, the exhaust valve and the bottom lid are firstly closed. The bone pieces are charged with the stainless steel rod through the top hole. Then the top lid is closed and the high-pressure tank is covered tightly. Then the electric power of the saturation steam boiler is turned on. When the temperature of the saturation steam boiler reaches 222.9°C and the pressure reaches 2.5MPa, the high- pressure valve is opened. When ,the temperatures of the high-pressure tank and the saturation steam boiler reach 222.9°C and their pressures reach 2.5MPa, i.e. the temperature and the pressure of the high-pressure tank and the saturation steam boiler are in equilibrium state, the electric power of the saturation steam boiler is turned off. After remaining the equilibrium state for 5 minutes, the high-pressure valve is closed and the exhaust valve is opened to let the liquefied bone juice come out.
In mixing step, the degreased milk powder is mixed with the bone juice. At this time, the milk powder is added in the amount of 10% of the bone juice. In sterilizing and cooling step, the mixture is boiled at the temperature of 100°C for 20 minutes, while being stirred. Then the mixture is rapidly cooled to the temperature of40~45°C.
In lactic acid fermenting step, a spore culture fluid in the amount of 1.25% of the mixture is added to the mixture. The spore culture fluid is made by mixing Lactobacillus Bulgaricus and Streptococcus in the ratio of 1 :4. And then the mixture is fermented at the temperature of 40~43°C for 4-5 hours.

Claims

Claims
1. Method for preparing an acidophilus milk from mammal bone, characterized by comprising the following steps;
a. cleaning the bones and cutting them into the 30mm-length pieces;
b. liquefying the bone pieces using a saturated steam at the temperature of 222.9°C and pressure of 2.5MPa;
c. mixing the degreased milk powder in the amount of 10% of bone juice with the liquefied bone juice;
d. sterilizing by means of boiling the mixture at the temperature of 100°C for 20 minutes and cooling rapidly to the temperature of 40~45°C; and e. adding a spore culture fluid in the amount of 1.25% of the mixture and lactic acid fermenting at the temperature of 40~43°C for 4-5 hours.
2. Method of preparing an acidophilus milk from mammal bone according to claim 1, characterized by maintaining the equilibrium state of the high-pressure tank and saturation steam boiler at the temperature of 222. °C and the pressure of 2.5MPa for 5 minutes.
3. Method of preparing an acidophilus milk from mammal bone according to claim 1, characterized that the spore culture fluid is made by mixing' Lactobacillus Bulgaricus and Streptococcus in the ratio 1 :4.
PCT/KP2012/000029 2011-08-16 2012-09-27 Acidophilus milk made from mammal bone and preparation method thereof WO2013024906A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KP201110002179 2011-08-16
KP271911 2011-08-16

Publications (2)

Publication Number Publication Date
WO2013024906A2 true WO2013024906A2 (en) 2013-02-21
WO2013024906A3 WO2013024906A3 (en) 2013-04-25

Family

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Family Applications (1)

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WO (1) WO2013024906A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1413596A (en) * 2002-03-16 2003-04-30 赵伟洁 Yoghurt containing ossein protein capsule, chewing tablet and its preparation method
CN1413478A (en) * 2002-03-16 2003-04-30 赵伟洁 Yoghurt containing bone protein liquid and preparation method
KR20050011989A (en) * 2003-07-24 2005-01-31 학교법인 인하학원 Yogurt-based supplement with condonopsis lanceolata trautv
CN101028072A (en) * 2006-03-02 2007-09-05 李洪军 Method for processing biological bone calcium nutrient health-care food
CN101554385A (en) * 2008-04-10 2009-10-14 四川省中医药科学院 Scalper bone chelate complex as well as preparation method and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1413596A (en) * 2002-03-16 2003-04-30 赵伟洁 Yoghurt containing ossein protein capsule, chewing tablet and its preparation method
CN1413478A (en) * 2002-03-16 2003-04-30 赵伟洁 Yoghurt containing bone protein liquid and preparation method
KR20050011989A (en) * 2003-07-24 2005-01-31 학교법인 인하학원 Yogurt-based supplement with condonopsis lanceolata trautv
CN101028072A (en) * 2006-03-02 2007-09-05 李洪军 Method for processing biological bone calcium nutrient health-care food
CN101554385A (en) * 2008-04-10 2009-10-14 四川省中医药科学院 Scalper bone chelate complex as well as preparation method and application thereof

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