WO2013013214A1 - Procédé de détection de micro-organismes - Google Patents

Procédé de détection de micro-organismes Download PDF

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Publication number
WO2013013214A1
WO2013013214A1 PCT/US2012/047737 US2012047737W WO2013013214A1 WO 2013013214 A1 WO2013013214 A1 WO 2013013214A1 US 2012047737 W US2012047737 W US 2012047737W WO 2013013214 A1 WO2013013214 A1 WO 2013013214A1
Authority
WO
WIPO (PCT)
Prior art keywords
apparatus system
particle
lanthanide
analyte
europium
Prior art date
Application number
PCT/US2012/047737
Other languages
English (en)
Inventor
Jaean Jung
Young Ho Choi
Seung Jae Baek
Hyeonsuk KIM
Tae-Hee Koo
Byeong-gap HWANG
Won-Jung Kim
Eun-jeong JANG
Jingeun RHEE
Heejun Kim
Original Assignee
Access Bio, Inc.
Medisensor, Inc.
Terawave, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Access Bio, Inc., Medisensor, Inc., Terawave, Inc. filed Critical Access Bio, Inc.
Publication of WO2013013214A1 publication Critical patent/WO2013013214A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody

Abstract

La présente invention concerne un procédé de dosage de Chlamydia, selon lequel des nanoparticules d'europium et un anticorps anti-Chlamydia sont conjugués au moyen d'une liaison amide pour déclencher une réaction immunitaire contre un antigène de Chlamydia. Ainsi, le conjugué réagit de manière sensible à l'antigène de Chlamydia pour détecter une quantité même infime de l'antigène de Chlamydia. Un appareil à fluorescence en temps résolu (TRF) fait appel à la TRF pour détecter un signal produit par l'europium. Étant donné que l'europium a une fréquence d'émission relativement longue, on obtient une réaction plus sensible et précise, en termes de détection de la fluorescence ou des effets de fond, qu'avec une courte longueur d'onde. Un objet de la présente invention concerne un lecteur de fluorescence ayant une structure simple, offrant une conception peu coûteuse.
PCT/US2012/047737 2011-07-20 2012-07-20 Procédé de détection de micro-organismes WO2013013214A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161510019P 2011-07-20 2011-07-20
US61/510,019 2011-07-20

Publications (1)

Publication Number Publication Date
WO2013013214A1 true WO2013013214A1 (fr) 2013-01-24

Family

ID=47558521

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/047737 WO2013013214A1 (fr) 2011-07-20 2012-07-20 Procédé de détection de micro-organismes

Country Status (1)

Country Link
WO (1) WO2013013214A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105445449A (zh) * 2015-04-04 2016-03-30 吉林双正医疗科技有限公司 唾液尿酸快速半定量检测装置及其制备方法
WO2020016308A1 (fr) 2018-07-18 2020-01-23 Ecole Polytechnique Test a diffusion capillaire mettant en œuvre des nanoparticules inorganiques photoluminescentes
GB2609419A (en) * 2021-07-29 2023-02-08 Ams Int Ag Lateral flow test

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175812A1 (en) * 2000-03-20 2003-09-18 Abs (Analytical Biological Services, Inc.) Method for detecting an analyte by fluorescence
US20040219690A1 (en) * 2003-05-02 2004-11-04 Choi Young Ho Chromatographic assay system
US20080199851A1 (en) * 2006-02-21 2008-08-21 Richard Laswell Egan Methods and compositions for analyte detection

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175812A1 (en) * 2000-03-20 2003-09-18 Abs (Analytical Biological Services, Inc.) Method for detecting an analyte by fluorescence
US20040219690A1 (en) * 2003-05-02 2004-11-04 Choi Young Ho Chromatographic assay system
US20080199851A1 (en) * 2006-02-21 2008-08-21 Richard Laswell Egan Methods and compositions for analyte detection

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105445449A (zh) * 2015-04-04 2016-03-30 吉林双正医疗科技有限公司 唾液尿酸快速半定量检测装置及其制备方法
CN105548534A (zh) * 2015-04-04 2016-05-04 吉林双正医疗科技有限公司 心肌标志物的联合检测装置及其制备方法
WO2020016308A1 (fr) 2018-07-18 2020-01-23 Ecole Polytechnique Test a diffusion capillaire mettant en œuvre des nanoparticules inorganiques photoluminescentes
FR3084165A1 (fr) 2018-07-18 2020-01-24 Ecole Polytechnique Test a diffusion capillaire mettant en œuvre des nanoparticules inorganiques photoluminescentes
GB2609419A (en) * 2021-07-29 2023-02-08 Ams Int Ag Lateral flow test

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