WO2013003976A1 - 热对流聚合酶连锁反应装置 - Google Patents

热对流聚合酶连锁反应装置 Download PDF

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Publication number
WO2013003976A1
WO2013003976A1 PCT/CN2011/001093 CN2011001093W WO2013003976A1 WO 2013003976 A1 WO2013003976 A1 WO 2013003976A1 CN 2011001093 W CN2011001093 W CN 2011001093W WO 2013003976 A1 WO2013003976 A1 WO 2013003976A1
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WIPO (PCT)
Prior art keywords
polymerase chain
chain reaction
seat
test tube
thermal convection
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Application number
PCT/CN2011/001093
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English (en)
French (fr)
Inventor
苏城
邓秉华
Original Assignee
瑞基海洋生物科技股份有限公司
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Application filed by 瑞基海洋生物科技股份有限公司 filed Critical 瑞基海洋生物科技股份有限公司
Priority to EP11869069.2A priority Critical patent/EP2728010B1/en
Priority to KR1020147002660A priority patent/KR101624927B1/ko
Priority to CA2840492A priority patent/CA2840492C/en
Priority to CN201180072027.6A priority patent/CN103732757B/zh
Priority to PCT/CN2011/001093 priority patent/WO2013003976A1/zh
Publication of WO2013003976A1 publication Critical patent/WO2013003976A1/zh

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/54—Heating or cooling apparatus; Heat insulating devices using spatial temperature gradients
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B01L2400/0445—Natural or forced convection
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Definitions

  • the invention relates to a polymerase chain reaction apparatus, and more particularly to a thermoconvergence polymerase interlocking reaction apparatus.
  • PCR polymerase chain reaction
  • the thermal convection polymerase chain reaction method mainly embeds the bottom of the test tube containing the PCR mixture in hot water, and the rest of the test tube is exposed to room temperature air to dissipate heat, so that the PCR mixture has a decreasing temperature gradient from the bottom to the surface. For example, 97 ° C to 35 ° C to induce thermal convection, the PCR mixture is subjected to different temperature sections for different reaction steps.
  • One object of the present invention is to provide a thermal convection polymerase chain reaction apparatus which can reduce the heat generated by a heat source from interfering with heat dissipation from the middle to the upper section of the test tube.
  • Another object of the present invention is to provide a thermoconvection polymerase chain reaction apparatus which is applicable to a simultaneous polymerase chain reaction method.
  • the polymerase chain reaction device for holding a test tube, and the polymerase chain reaction is carried out in a test tube.
  • the polymerase chain reaction device package The utility model comprises a heat insulation seat and a heating seat, wherein the heat insulation seat has a body, a chamber inside the body and accommodating the bottom of the test tube, a side passage connecting the chamber and the outside, and an upper passage connecting the chamber and the outside, The test tube is placed through the side passage; the heating seat is disposed on the side passage of the heat insulation seat and is attached to the bottom of the test tube.
  • Figure 1 is a perspective view of a first preferred embodiment of the present invention
  • Figure 2 is another perspective view of the first preferred embodiment of the present invention.
  • Figure 3 is a cross-sectional view taken along line 3-3 of Figure 1;
  • Figure 4 is a schematic view of the operation of the first preferred embodiment of the present invention.
  • test tube rack 60 test tube rack 62 housing hole
  • the thermal convection polymerase chain reaction device 10 provided by the preferred embodiment of the present invention mainly includes a heat insulating seat 20, a heating base 30, a light emitting module 40, a heat conducting base 50, and a heat conducting block. Test tube rack 60 and a power unit 70.
  • the heat insulator 20 has a body 22, a chamber 24 is disposed inside the body 22 for accommodating the bottom 121 of the test tube 12, a side passage 261 is connected to the chamber 24 and the outside, and an upper passage 262 is connected to the chamber 24 and the outside.
  • the test tube 12 is placed through; the heating seat 30-end is disposed through the side channel 261 of the heat insulating seat 20, and is attached to the bottom portion 121 of the test tube 12.
  • the heat insulation seat 20 is made of plastic or ceramic, wherein the plastic can be a composite of nylon and glass fiber or a composite of acrylic and ABS plastic, so the heat insulation seat has low thermal conductivity; the heating seat 30 is made of metal such as copper. Therefore, the heating seat has high thermal conductivity.
  • the heating base 30 generates heat by electric power, can avoid the generation of high-temperature water vapor, and the gap between the upper passage 262 and the test tube 12 is small, even if the heating seat 30 heats the air in the chamber 24, the hot gas is in the middle of the test tube 12. The degree of interference from the heat dissipation of 122 to the upper section 123 is also small.
  • the light-emitting module 40 is located under the heat insulator 20, as shown in FIG. 3, and the heat insulator 20 has a lower channel 263. Light emitted by the light emitting module 40 enters the chamber 24.
  • the light emitting module 40 can emit a specific wavelength of light to illuminate the PCR mixture in the test tube 12 through the lower channel 263, and excite the fluorescent particles in the PCR mixture to fluoresce, and then use an optical fiber 80 and a light sensing device 90.
  • the total amount of product in the mixture can be known by detecting the fluorescence intensity in the tube 12.
  • the present invention heats the bottom 121 of the test tube 12 with the hot seat 30 instead of the hot water, the light emitted by the light emitting module 40 is not disturbed by the hot water, and since the heating base 40 is disposed on the side of the test tube 12, the light emitting module 40 is disposed in the test tube 12. Below, the light emitted from the light-emitting module 40 can be irradiated to the entire test tube. Therefore, the present invention can be applied to a simultaneous polymerase chain reaction reaction method to attain the object of the present invention.
  • the light emitting module 40 can adopt an LED module, a halogen lamp, a xenon lamp or a xenon lamp, and a filter 43 is disposed between the chamber 24 and the light emitting module 40 to filter the light emitted by the LED module, and only specific The light of the wavelength illuminates the test tube 12.
  • the present invention provides a heat conducting seat 50.
  • the heat conducting base 50 has a body 52 and a passage 54, wherein the body 52 is disposed above the heat insulating seat 20; The body 52 is in communication with the upper passage 262 of the heat insulating seat 20 for receiving the test tube 12.
  • the middle section 122 to the upper section 123 are located in the passage 54 of the heat conducting seat 50.
  • the heat conducting seat 50 is made of a metal such as an aluminum alloy or a copper alloy, the heat of the PCR mixture in the test tube 12 passes through the surrounding air. Passing to the heat transfer seat 50, it can quickly dissipate, so that the PCR mixture gradually lowers the temperature when it is convected upwards.
  • the PCR mixture can be lowered to the temperature required for the extension reaction, such as 72 ° C, and the surface is PCR.
  • the temperature of the mixture can be lowered to a temperature lower than the temperature required for the adhesion reaction of the primer, such as 35 ° C.
  • the convection is repeated, and the PCR reaction can be continuously performed.
  • the chamber 24 of the insulating seat 20, the upper passage 262 and the passage 54 of the heat conducting seat 50 constitute a reaction chamber 56, and the heat flow in the reaction chamber 56 is guided to the outside by the heat conducting seat 50, so that the heating seat as a whole 30, heat energy is input from the bottom 121 of the test tube, and the heat transfer seat 50 derives the thermal energy of the middle section 122 and the upper section 123 and the heat of the hot gas from the passage 262 of the heat insulator 20, so that the PCR mixture in the test tube 56 and the test tube 12 are in the reaction chamber 56.
  • the outside air maintains a temperature gradient that gradually decreases from bottom to top.
  • the heat insulator 20 isolates the direct heat exchange between the external environment and the reaction chamber 56, and the heat transfer seat 50 conducts the internal heat flow.
  • the environmental influence outside the reaction chamber 56 can be effectively eliminated, and a stable temperature gradient in the reaction chamber 56 is formed, thereby The thermal convection polymerase chain reaction can proceed stably.
  • the channel 54 of the heat conducting block 50 has a large diameter section 541, and a small diameter section 542 is located below the large diameter section 541.
  • the PCR mixture located in the small diameter section 542 dissipates heat faster, and the upper large diameter section 541 is slower.
  • the PCR reaction was carried out at 7 different ambient temperatures, and the temperature of the heating block 30 was set between 104 and 15 ° C, so that the temperature of the PCR mixture in the bottom 121 of the test tube reached 93 to 97 ° C, and the heat conducting seat 50 The temperature was measured to be 36 to 53 ° C, and the temperature of the surface of the mixture was between 36 and 53 ° C, allowing the PCR reaction to proceed efficiently.
  • a test tube rack 60 is disposed above the heat conducting base 50, and the test tube rack 60 has a receiving hole 62 for inserting the test tube 12.
  • the shape of the receiving hole 62 is complementary to the upper portion 123 of the test tube, so that when the test tube 12 is placed in the receiving hole 62, it does not move relative to the test tube holder 60.
  • a power unit 70 can be coupled to the heating base 30 to move the heating base 30 between a contact position P1 and a disengagement position P2.
  • the power unit 70 can be used without being limited to a motor, a pneumatic cylinder, or a hydraulic cylinder.
  • the heating base 30 can contact the bottom portion 121 of the test tube and heat the bottom portion 121.
  • the power unit 70 moves the heating base 30 to the disengaging position P2
  • the heating base 30 is separated from the test tube 12, thereby stopping the heating of the test tube bottom 121.
  • the structure of the device can also be modified in various ways.
  • the LED module and the filter 43 can also be disposed in the lower channel 263, so that the volume of the device can be reduced;
  • a laser module is not provided with a filter 43.
  • Structural variations based on the inventive concept are intended to be covered by the scope of the invention.
  • the device of the invention can reduce the heat generated by the heat source to interfere with the heat dissipation from the middle to the upper part of the test tube, and the synchronous polymerase chain reaction method can be applied.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

提供一种热对流聚合酶连锁反应装置。该热对流聚合酶连锁反应装置是用于承置一试管，使聚合酶连锁反应于该试管内进行，该聚合酶连锁反应装置包含有一绝热座以及一加热座，其中该绝热座具有一本体，一容室位于该本体内部用于容纳试管底部，一侧通道连通该容室与外界，以及一上通道连通该容室与外界，用于供该试管穿置；该加热座穿设于该绝热座的侧通道，用于贴抵于试管的底部。该装置可减少热源产生的高温干扰位于试管中段至上段的PCR混合液的散热，并适用同步聚合酶连锁反应方法。

Description

热对流聚合酶连锁反应装置 技术领域
本发明与聚合酶连锁反应仪器有关， 特别是指一种热对流聚合酶连 锁反应装置。
背景技术
在生技领域中， 聚合酶连锁反应（PCR)已成为一项扩增特定核酸序 列的成熟技术， 此反应过程需要三个主要步骤： 变性反应、 引子黏合反 应及延展反应， 其各需不同的反应温度。 变性反应的温度一般介于 90 至 97 °C之间； 引子黏合反应的温度是依据所用引子的解链温度而选择， 一般是介于 35至 65 °C之间； 延展反应的一般温度为 72 °C。
热对流聚合酶连锁反应方法主要是将装有 PCR混合液的试管底部埋 置于热水中， 试管的其余部分则暴露在室温空气中散热， 使 PCR混合液 底部至表面呈渐减的温度梯度如 97 °C至 35 °C， 以诱发热对流， 使 PCR 混合液经历不同的温度区段而进行不同的反应步骤。
但是， 由于热水表面会产生高温蒸气并往上对流， 将干扰该试管中 段至上段的散热， 使混合液表面较难达到符合引子黏合反应所需的温 度。
另外， 在同步聚合酶连锁反应（rea l-t i me PCR)方法中为实时侦测 反应是否完成， 需向 PCR混合液中添加荧光染剂， 并用镭射光由试管下 方照射该混合液， 侦测反应混合液中荧光的强度， 以得知 PCR反应是否 完成， 由于现有反应装置是在试管底端用热水加热， 热水会严重干扰镭 射光的照射， 使现有装置无法执行同步聚合酶连锁反应。
发明内容
本发明的一个目的是提供一种热对流聚合酶连锁反应装置， 该装置 可减少热源产生的热气干扰试管中段至上段的散热。
本发明的另一个目的在于提供一种热对流聚合酶连锁反应装置， 其 可适用同步聚合酶连锁反应方法。
为实现上述目的， 本发明所提供的聚合酶连锁反应装置， 是用以承 置一试管， 使聚合酶连锁反应于试管内进行， 该聚合酶连锁反应装置包 含有一绝热座和一加热座， 其中绝热座具有一本体、 一位于本体内部且 容纳试管底部的容室、 一连通容室与外界的侧通道， 和一连通容室与外 界的上通道， 用以供该试管穿置； 所述加热座穿设于所述绝热座的侧通 道， 贴抵于试管的底部。 附图说明
图 1为本发明第一较佳实施例的立体图；
图 2为本发明第一较佳实施例的另一立体图;
图 3为图 1沿 3-3剖线的剖视图；
图 4为本发明第一较佳实施例的动作示意图。
主要附图标记说明如下：
10热对流聚合酶连锁反应装置
12试管 121底部 122中段
123上段
20绝热座 22本体 24容室
261侧信道 262上信道
263下信道
30加热座
40发光模块 43滤光片
50导热座 52本体 54通道
541大径段 542小径段
56反应室
60试管架 62容置孔
70动力装置
80光纤
90光感测装置
P 1接触位置
P2脱离位置 具体实施方式 为了更了解本发明的特点所在， 现举以下一较佳实施例并配合附图 说明如下：
如图 1至图 3所示， 本发明的较佳实施例所提供的热对流聚合酶连 锁反应装置 10主要包含有一绝热座 20、 一加热座 30、 一发光模块 40、 一导热座 50、 一试管架 60和一动力装置 70。
绝热座 20具有一本体 22、 一容室 24位于本体 22内部用于容纳试 管 12的底部 121、一侧通道 261连通容室 24与外界，以及一上通道 262 连通容室 24与外界， 用于供试管 12穿置； 加热座 30—端穿设于绝热 座 20的侧通道 261， 贴抵于试管 12的底部 121。
绝热座 20 由塑料或陶瓷所制成， 其中塑料可为尼龙与玻璃纤维的 复合物或压克力与 ABS塑料的复合物， 因此该绝热座具低导热性； 加热 座 30由金属如铜制成， 因此该加热座具有高导热性。 加热座 30是以电 力产生热能， 可避免高温水蒸气的产生， 且上通道 262与试管 12之间 的空隙很小， 即使加热座 30会将容室 24内的空气加热， 热气对试管 12 中段 122至上段 123的散热的干扰程度也不大。
为实时侦测每次 PCR循环后的产物总量， 也即进行同步聚合酶连锁 反应， 本实施例中发光模块 40位于绝热座 20下方， 如图 3所示， 且绝 热座 20具有一下通道 263供发光模块 40所发出的光进入容室 24。发光 模块 40可发出一特定波长的光经过下通道 263照射试管 12中 PCR混合 液， 并激发 PCR混合液中具有荧光性质的粒子使其发出荧光， 再用一光 纤 80和一光感测装置 90侦测试管 12 中的荧光强度便可得知混合液中 的产物总量。
由于本发明是以加热座 30而非热水加热试管 12底部 121， 所以发 光模块 40所发出的光不受热水干扰，并且由于加热座 40设置于试管 12 侧面， 发光模块 40设置于试管 12下方， 使发光模块 40所发出的光可 照射到整支试管， 因此， 本发明可适用同步聚合酶连锁反应方法， 达到 本发明的目的。
实际使用时， 发光模块 40可采用 LED模块、 卤素灯、 氚气灯或氙 气灯， 且容室 24与发光模块 40之间设有一滤光片 43 以过滤该 LED模 块发出的光， 只允许特定波长的光照射试管 12。 为加强试管中段 122至上段 123的散热， 本发明设置导热座 50， 如 图 3所示， 导热座 50具有一本体 52以及一通道 54， 其中本体 52设于 绝热座 20上方； 通道 54穿设于本体 52且与绝热座 20的上通道 262连 通， 用于供试管 12穿置。 反应进行时， 试管中段 122至上段 123位于 导热座 50的通道 54中， 由于导热座 50是由易散热材质如铝合金、 铜 合金等金属制成， 试管 12 中 PCR混合液的热能通过周围空气传递至导 热座 50， 即可迅速逸散， 使 PCR混合液在向上对流时逐渐降低温度， 约 在中段 122时 PCR混合液可降至延展反应所需的温度如 72 °C，到达表面 时 PCR混合液温度更可降至低于引子黏合反应所需的温度如 35 °C，如此 反复循环对流， 即可使 PCR反应不断进行。
事实上， 绝热座 20的容室 24、 上通道 262 以及导热座 50 的通道 54构成一反应室 56， 且反应室 56内的热流由导热座 50导引至外界， 因此整体看来， 加热座 30由试管底部 121输入热能， 而导热座 50将试 管中段 122与上段 123的热能以及来自绝热座 20上通道 262 的热气的 热能导出， 使反应室 56内试管 12中的 PCR混合液以及试管 12外的空 气都维持一由下往上渐低的温度梯度。
换言之， 绝热座 20隔绝外界环境与反应室 56的直接热交换， 导热 座 50将内部热流导出， 如此， 可有效排除反应室 56外的环境影响， 形 成反应室 56 内稳定的温度梯度， 进而使热对流聚合酶连锁反应能稳定地 进行。
为使试管 12内的温度梯度更有利于 PCR反应进行， 导热座 50的通 道 54具有一大径段 541， 以及一小径段 542位于大径段 541下方。位于 小径段 542的 PCR混合液散热较快， 在上方的大径段 541则较慢， 经过 多次实验测试， 证实此结构可以使 PCR反应更有效率地进行， 实验分别 在 10至 40 °C间的 7个不同环境温度下进行 PCR反应，将加热座 30的温 度设定在 104至 1 15 °C之间， 使试管底部 121 内的 PCR混合液温度达到 93至 97 °C， 导热座 50的温度经过测量为 36至 53 °C， 而该混合液表面 的温度则在 36〜53 °C之间， 使 PCR反应得以有效率地进行。
为使试管 12稳固地插置于导热座 50与绝热座 20， 导热座 50上方 设置一试管架 60， 且试管架 60具有一容置孔 62用于供试管 12插置。 容置孔 62 的形状与试管上段 123互补， 因此试管 12置放于容置孔 62 时， 不会相对于试管架 60移动。
请参阅图 3和图 4，本发明可增设一动力装置 70与加热座 30连接， 使加热座 30可在一接触位置 P 1与一脱离位置 P2之间移动。 实际运作 时， 动力装置 70可采用而不限于马达、 气压缸、 油压缸， 当动力装置 70将加热座 30 移动至接触位置 P 1时， 加热座 30可接触试管底部 121 而加热位于底部 121 的 PCR混合液； 当动力装置 70将加热座 30 移动 至脱离位置 P2时， 加热座 30便脱离试管 12， 从而停止对试管底部 121 加热。
基于本发明的精神， 此装置的结构也可有多种变化方式， 例如： LED 模块与滤光片 43也可设于下通道 263 内， 如此可缩小此装置的体积； 发光模块 40 也可采用一镭射模块而不设滤光片 43。凡基于本发明构思 的结构变型， 均应为本发明申请专利范围所涵盖。 工业应用
本发明装置可减少热源产生的热气干扰试管中段至上段的散热， 且 可适用同步聚合酶连锁反应方法。

Claims

权利要求
1. 一种热对流聚合酶连锁反应装置， 用于承置一试管， 使聚合酶 连锁反应在所述试管内进行， 其特征在于： 该热对流聚合酶连锁反应装 置包含有：
一绝热座， 具有一本体、 一位于本体内部且容纳试管底部的容室、 一连通容室与外界的侧通道、 和一连通容室与外界的上通道， 用于供所 述试管穿置；
一加热座， 穿设于绝热座的侧通道， 贴抵于试管的底部。
2. 根据权利要求 1 所述的热对流聚合酶连锁反应装置， 其特征在 于： 所述绝热座的材质为塑料或陶瓷。
3. 根据权利要求 1 所述的热对流聚合酶连锁反应装置， 其特征在 于： 所述加热座的材质为金属。
4. 根据权利要求 1 所述的热对流聚合酶连锁反应装置， 其特征在 于： 所述装置还包含有一位于绝热座下方的发光模块， 且所述绝热座还 具有一供所述发光模块所发出的光进入所述容室的下通道。
5. 根据权利要求 4所述的热对流聚合酶连锁反应装置， 其特征在 于： 所述装置还包含有一滤光片设于所述容室与所述发光模块之间， 且 所述发光模块为一 LED模块、 卤素灯、 氚气灯或氙气灯。
6. 根据权利要求 4所述的热对流聚合酶连锁反应装置， 其特征在 于： 所述发光模块为一镭射模块。
7. 根据权利要求 1 所述的热对流聚合酶连锁反应装置， 其特征在 于： 所述装置还包含有一导热座， 所述导热座具有一本体设于所述绝热 座上方， 和一通道穿设于所述本体且与所述绝热座的上通道连通， 用于 供所述试管穿置。
8. 根据权利要求 7所述的热对流聚合酶连锁反应装置， 其特征在 于： 所述导热座的材质为金属。
9. 根据权利要求 7所述的热对流聚合酶连锁反应装置， 其特征在 于： 所述绝热座的容室、 上通道以及所述导热座的通道构成一反应室， 且所述反应室的热能由所述导热座导引至外界。
10. 根据权利要求 Ί所述的热对流聚合酶连锁反应装置，其特征在 于： 所述通道具有一大径段， 和一位于所述大径段下方的小径段。
1 1. 根据权利要求 7所述的热对流聚合酶连锁反应装置，其特征在 于： 所述装置还包含有一试管架， 设置于所述导热座上方， 且所述试管 架具有一供试管插置的容置孔。
12. 根据权利要求 1-1 1 中任一所述的热对流聚合酶连锁反应装 置， 其特征在于： 所述装置中还包含有一动力装置， 与所述加热座连接， 使所述加热座在一接触位置与一脱离位置之间移动。
PCT/CN2011/001093 2011-07-01 2011-07-01 热对流聚合酶连锁反应装置 WO2013003976A1 (zh)

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CA2840492A CA2840492C (en) 2011-07-01 2011-07-01 Thermal convection polymerase chain reaction device
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