WO2012131731A1

WO2012131731A1 - A herbal formulation to treat coccidiosis

Info

Publication number: WO2012131731A1
Application number: PCT/IN2012/000231
Authority: WO
Inventors: Ibetombi Devi OINAM
Original assignee: Ibetombi Devi OINAM
Priority date: 2011-03-31
Filing date: 2012-03-30
Publication date: 2012-10-04
Also published as: WO2012131731A8

Abstract

The present invention relates to a formulation to treat coccidiosis. More particularly, present invention relates to a formulation prepared from Allium Odorum, Allium sativum, Tinospora cordifolia, Adhatoda vasica and Tridax procumbens to treat coccidiosis. The formulation prepared according to present invention is effective for the treatment of coccidiosis. The herbal composition used in present invention provides relief to the poultry without any side effect. The present invention provides an effective and low cost method for treating coccidiosis.

Description

"A HERBAL FORMULATION TO TREAT COCCIDIOSIS"

FIELD OF INVENTION

The present invention relates to a formulation, to treat coccidiosis. More particularly, present invention relates to a formulation prepared from Allium Odorum, Allium sativum, Tinospora cordifolia, Adhatoda vasica and Tridax procumbens to treat coccidiosis.

BACKGROUND OF THE INVENTION

Coccidiosis is a parasitic disease of tropical and subtropical regions caused by the a protozoan type parasite that causes tissue damage in the intestinal tract. Symptoms include fever, malaise, abdominal discomfort, and watery diarrhea. The infection is usually self-limited, lasting 1 to 2 weeks, but occasionally it persists, resulting in malabsorption syndrome and, rarely, death. Coccidia can also damage the immune system and leave poultry more vulnerable to pathogens like Clostridium, Salmonella and E. coli. Eimeria and Isospora are the two genera that are often referred to as "coccidia." These two genera contain a large number of species that infect a variety of animals throughout the world. The diseases caused by these microscopic protozoal parasites are referred to collectively as coccidiosis, and they vary tremendously in virulence. Domestic poultry and birds are affected by coccidia called Eimeria (scientific name). There are different types of host specific species of Eimeria. Nine species of Eimeria infect chickens. Eimeria necatrix and E tenella are the most pathogenic in chickens. Seven species infect turkeys - the big three of concern are Eimeria meleagrimitis, E. adenoeides, and E. gallapovonis.

Anticoccidial medication is commonly added to poultry feed as a preventative measure against

the disease. However, some medications, like ionophors - Coban, Avatec, allow leakage of oocysts, so the birds can build up an immunity to the parasite. Also, the birds can ingest more coccidia oocysts from the litter. Treatment of coccidiosis is used to control an outbreak. Amprolium, sulf adimethoxine, and sulfaquinoxaline are effective against coccidia as a treatment, however toxicity and withdrawal times are a matter of concern with sulfa-drugs. Allium odorum of Chinese Chives is a species in the genus Allium (onion) which contains 824 species and belongs to family Alliaceae (Garlic Family).

Chemical composition in various parts of this plant are: the leaves and bulbs contain sulfur compounds, saponin and bitter substances. The seeds yield saponin and alkaloids. The leaves and the bulbs of this plant possess antibacterial properties. They are useful for the treatment of haemoptysis, epistaxis, cough, sore throat, dyspepsia, haematometra, asthma, dysentery and oxyuriasis. The usual dose is 20 to 30g per day in the form of a decoction. They are also used in an anti-inflammatory poultice. The seeds are active on spermatorrhoea, haematuria, incontinence, lumbago, arthrodynia and metrorrhoea. The usual dose is 6 to 12g per day in the form of a decoction (http://enervon.com). According to the Chinese literature, it is supposed to nourish and purify the blood , to act as a cordial and effective against poisonous bites of dogs, serpents, insects, haemorrhages of every sorts and spermatorrhoea (Chinese medicinal herbs: a modern edition of a classic sixteenth-century manual by Shizhen Li, Porter Smith, George Arthur Stuart). Allium Sativum or Garlic is a species in the onion genus. It is a kind of a plant widely used in the Indian kitchen. Garlic has been found to have antibacterial, antiviral, and antifungal activity. Garlic is also alleged to help regulate blood sugar levels. It is found to be effective against infections, coughs and cold and diabetes. Tinospora cordifolia, commonly known as Guduchi or Giloy is an herbaceous vine of the family Meninspermaceae, indigenous to the tropical areas of India. It is a climbing shrub and is often grown on mango or neem trees. In traditional medicinal system, it is used for the treatment of gout, high uric acid, skin problems, autoimmune disorders and antipyretic. The plant Adhatoda vasica commonly known as vasaka belongs to a family Acanthaceae. It contains vasicine, a quinazoline alkaloid which is responsible for its medicinal property. The plant is distributed all over the plains of India & in lower Himalayan ranges. Traditionally,the plant is used for bleeding due to idiopathic thrombocytopenic purpura, local bleeding due to peptic ulcer, piles, menorrhagia, relief in pyorrhoea and for bleeding gums by locally application, relieves or eases muscular spasms, cramps or convulsions and lowers blood pressure.(www.hi^'//green.com) Tridax procumbers, commonly known as Jayanti veda or ghamra in India and belongs to the family Asteraceae. It is a species of flowering plant and is best known as a widespread weed. It is native to tropical, subtropical, and mild temperate regions. Tridax procumbens is known for several potential therapeutic activities like antiviral anti oxidant, antibiotic efficacies, wound healing activity, insecticidal and antiinflammatory activity. (Suseela L; saravathy, A; Brindha, P(2002), Journal of Phytological Research 15 (2): 141-147.) Some reports from tribal areas state that the leaf juice can be used to cure fresh wounds, to stop bleeding, as a hair tonic.

OBJECTIVES OF THE INVENTION

The main object of the present invention to provide a herbal formulation for curing coccidiosis in poultry.

Another objective of present invention is to provide an effective and low cost method for treatment of curing coccidiosis in poultry having no harmful side effect.

SUMMARY OF THE INVENTION

Accordingly, the present invention provides a formulation for treating coccidiosis. More particularly, the present invention relates to a herbal formulation for treating coccidiosis comprising Allium odorum, Allium sativum, Tinospora cordifolia, Adhatoda vasica and Tridax procumbens, and a method of preparation thereof.

DESCRIPTION OF THE INVENTION

The present invention provides ^ herbal formulation for treating coccidiosis. More particularly, the present invention relates to a herbal formulation for treating coccidiosis comprising Allium odorum, Allium sativum, Tinospora cordifolia, Adhatoda vasica and Tridax procumbens and a method of preparation thereof. The present invention provides a herbal formulation comprising plants Allium odorum, Allium sativum, Tinospora cordifolia, Adhatoda vasica and Tridax procumbens, and is prepared by a process comprising the steps of :

(a) washing plant material derived from the plants Allium odorum, Allium sativum, Tinospora cordifolia, Adhatoda vasica and Tridax procumbens;

(b) mixing the plant material of step (a) in water in an amount sufficient to make paste of the plant material;

(c) grinding the mixture obtained from step (b) to obtain the herbal formulation.

The plant material is collected, washed, mixed and then is ground to paste. Water is added for grinding the plant material in a sufficient quantity to make a paste of the plant material.

In an embodiment, the plant material is selected from the group consisting of a whole plant, a rhizome, a leaf, a seed, a root, a stem, a bark, a flower or a fruit. In another preferred embodiment, the plant material is rhizome of Allium odorum, leaves of Allium sativum, leaves of Tinospora cordifolia, leaves of Adhatoda vasica and leaves of Tridax procumbens.

In another embodiment, the plant materials are mixed in a ratio of 2-4 parts of Allium odorum, 1-2 parts of Allium sativum, 2-3 parts of Tinospora cordifolia, 1-2 parts of Adhatoda vasica and 1-2 parts of Tridax procumbens.

The composition of the present invention may be administered as such or in admixture with a pharmaceutically acceptable vehicle, adjuvants, inactive excipients, diluents or lubricants.

Non-limiting examples of pharmaceutically acceptable vehicle, adjuvants, inactive excipients, diluents or lubricants which can be used in the composition of the present invention include: cellulose, substituted cellulose, calcium carbonate, dicalcium phosphate, starches, lactose, modified food starches, dextrose, calcium sulfate, magnesium carbonate, magnesium stearate, stearic acid, glycerin, vegetable oils, polysorbates or lecithin. , The herbal formulation in a dosage of 400-600 mg/bird is given to the poultry once every morning till recovery.

EXAMPLE

Formulation of the present invention was prepared from the rhizome of plant Allium odorum and leaves of each of the plants Allium sativum, Tinospora cordifolia, Adhatoda vasica and Tridax procumbens. 160g of rhizome of Allium odorum, 70g of Allium sativum, 130g of Tinospora cordifolia, 75g of Adhatoda vasica and 75g of Tridax procumbens were washed, mixed and grounded to make paste using sufficient amount of water. The medication is fed to birds as feed mixture with a dosage of 400 mg per bird. RESULTS:

Unsporulated Oocysts are to be randomly allocated to 4 concentrations (v/v; 0%, 15%, 30%, and 45%) of extracts. The effect of herbal drug on inhibition of sporulation were studied and found significant.

In-vivo studies

40 numbers of day old broiler chicks are to be divided into 2 groups Tl to T7 consisting of 20 chicks in each group. The population were infected and therapeutic dosage was administered from 12 day old till the end of trial (6 week). The control population was not administered any drug. On 14th day birds are infected with sporulated oocysts of Eimeria tenella or mixed Eimeria spp., On 21 DPI, 50% birds in each group were sacrificed and studied for gross lesion score, microscopical lesion scoring as per standard protocol. It was found that test bird population did not suffer much in comparison to control. Body weight and Feed efficiency of the birds after 1, 7, 12, 14, 16, 21, 28, 35 & 42 days were also recorded and found significant difference among test and control population. ADVANTAGES OF THE INVENTION

The formulation prepared according to present invention is effective for the treatment of coccidiosis. The herbal composition used in present invention provides relief to the poultry without any side effect. The present invention provides an effective and low cost method for treating coccidiosis .

Claims

We Claim:

1. A herbal formulation for the treatment of coccidiosis, comprising plants Allium odorum, Allium sativum, Tinospora cordifolia, Adhatoda vasica and Tridax procumbens and is prepared by a process comprising the steps of :

2. The herbal formulation as claimed in claim 1, wherein the plant material is selected from the group consisting of a whole plant, a rhizome, a leaf, a seed, a root, a stem, a bark, a flower or a fruit.

3. The herbal formulation as claimed in claim 1, wherein the plant material is rhizome of Allium odorum, leaves of Allium sativum, leaves of Tinospora cordifolia, leaves of Adhatoda vasica and leaves of Tridax procumbens.

4. The herbal formulation as claimed in step (b) of claim 1, wherein the plant material is mixed in a ratio of 2-4 parts of Allium odorum, 1-2 parts of Allium sativum, 2-3 parts of Tinospora cordifolia, 1-2 parts of Adhatoda vasica and 1-2 parts of Tridax procumbens.

5. The herbal formulation as claimed in any of the preceding claims, wherein the herbal composition is in admixture with a pharmaceutically accepted vehicle or an adjuvant.