WO2012112458A3 - Compositions and methods for increasing reprogramming efficiency - Google Patents

Compositions and methods for increasing reprogramming efficiency Download PDF

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Publication number
WO2012112458A3
WO2012112458A3 PCT/US2012/024902 US2012024902W WO2012112458A3 WO 2012112458 A3 WO2012112458 A3 WO 2012112458A3 US 2012024902 W US2012024902 W US 2012024902W WO 2012112458 A3 WO2012112458 A3 WO 2012112458A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
reprogramming efficiency
pluripotent cell
efficiency
Prior art date
Application number
PCT/US2012/024902
Other languages
French (fr)
Other versions
WO2012112458A2 (en
Inventor
Ambrose WILLIAMS
Wenbin Deng
Original Assignee
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Publication of WO2012112458A2 publication Critical patent/WO2012112458A2/en
Publication of WO2012112458A3 publication Critical patent/WO2012112458A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/724—Glycosyltransferases (EC 2.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Abstract

The present invention relates to methods for enhancing the efficiency of reprogramming a non-pluripotent cell to a pluripotent cell, comprising expressing in the cell an effective amount of pluripotency factors and an effective amount of PARP-1 or an equivalent thereof, thereby enhancing the efficiency of reprogramming the non-pluripotent cell to the pluripotent cell.
PCT/US2012/024902 2011-02-14 2012-02-13 Compositions and methods for increasing reprogramming efficiency WO2012112458A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161442554P 2011-02-14 2011-02-14
US61/442,554 2011-02-14

Publications (2)

Publication Number Publication Date
WO2012112458A2 WO2012112458A2 (en) 2012-08-23
WO2012112458A3 true WO2012112458A3 (en) 2012-11-15

Family

ID=46673107

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/024902 WO2012112458A2 (en) 2011-02-14 2012-02-13 Compositions and methods for increasing reprogramming efficiency

Country Status (1)

Country Link
WO (1) WO2012112458A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104419683A (en) * 2013-09-10 2015-03-18 中国科学院生物物理研究所 Method for preparing autologous induced pluripotent stem cells of patient with Fanconi anemia and application of method
EP3690032A1 (en) * 2013-09-20 2020-08-05 Lonza Ltd. Methods for nuclear reprogramming of cells
JP2017513517A (en) * 2014-03-19 2017-06-01 ブイセル セラピューティックス，インコーポレイテッド Methods involving pluripotent cells
CN108070563A (en) * 2016-11-17 2018-05-25 中国科学院大连化学物理研究所 A kind of preparation method of inductive pluripotent stem cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090068742A1 (en) * 2005-12-13 2009-03-12 Shinya Yamanaka Nuclear Reprogramming Factor
US20090304646A1 (en) * 2007-06-15 2009-12-10 Kazuhiro Sakurada Multipotent/Pluripotent Cells and Methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090068742A1 (en) * 2005-12-13 2009-03-12 Shinya Yamanaka Nuclear Reprogramming Factor
US20090304646A1 (en) * 2007-06-15 2009-12-10 Kazuhiro Sakurada Multipotent/Pluripotent Cells and Methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FURONG GAO ET AL.: "PARP1 poly(ADP-ribosyl)ates Sox2 to Control Sox2 Protein Levels and FGF4 Expression during Embryonic Stem Cell Differentiation", J BIOL CHEM., vol. 284, no. 33, 16 June 2009 (2009-06-16), pages 22263 - 22273 *
VIMAL SELVARAJ ET AL.: "PARP-1 Deficiency Increases the Severity of Disease in a Mouse Model of Multiple Sclerosis", J BIOL CHEM., vol. 284, no. 38, 18 September 2009 (2009-09-18), pages 26070 - 26084 *

Also Published As

Publication number Publication date
WO2012112458A2 (en) 2012-08-23

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