WO2012104176A1

WO2012104176A1 - Use of browned glucose as a feed substrate

Info

Publication number: WO2012104176A1
Application number: PCT/EP2012/051084
Authority: WO
Inventors: Niels Banke
Original assignee: Novozymes A/S
Priority date: 2011-01-31
Filing date: 2012-01-25
Publication date: 2012-08-09
Also published as: US9347080B2; CN103339251A; EP2670839B1; CA2823270A1; CN103339251B; US20130309722A1; ES2656049T3; EP2670839A1

Abstract

A method for fermenting a microorganism, producing a compound of interest, in a culture medium comprising: adding a browned glucose solution to the culture medium, wherein the browned glucose solution is a glucose solution that has been acid treated and heated to a temperature of at least 90 degrees Celsius, and wherein the glucose solution has a concentration of at least 500 g/l.

Description

Use of browned glucose as a feed substrate

TECHNICAL FIELD

The present invention relates to a method of increasing the yield of a compound of interest in a fermentation using browned glucose as a feed substrate.

BACKGROUND ART

Commercially it is of key importance continuously to increase the yiel d of a compound of interest produced by microbial fermentation in industrial scale.

If productivity can be increased this will liberate production capacity for other compounds and reduce the need for new investments in production.

It is known (Process Biochemistry, vol. 27, no. 6, 1992, page 327-334) that acid hydrolysed starch may be an inducer for cellulase production by Trichoderma reesei. SUMMARY OF THE INVENTION

It has surprisingly been found that the yield of a compound of interest may be increased very significantly by adding a browned glucose solution to the culture medium, so we claim:

A method for fermenting a microorganism, producing a compound of interest, in a culture medium comprising:

adding a browned glucose solution to the culture medium, wherein the browned glucose solution is a glucose solution that has been acid treated and heated to a temperature of at least 90 degrees Celsius, and wherein the glucose solution has a concentration of at least 500 g/l.

DETAILED DISCLOSURE OF THE INVENTION

The present invention deals with increasing the yield of a product of interest in industrial fermentations.

The microorganism may be any microorganism useful for industrial fermentations, in particular a bacterium or a fungus.

Bacterium

The bacterium expressing the compound of interest according to the invention may be a bacterium of any genus.

In a preferred embodiment, the compound of interest may be obtained from a gram positive bacterium, in particular from a Bacillus or a Streptomyces strain.

In a preferred embodiment the Bacillus strain is selected from the group consisting of Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillus coagulans, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus megaterium, Bacillus stearothermophilus, Bacillus subtilis, and Bacillus thuringiensis.

In another preferred embodiment the Streptomyces strain is selected from the group consisting of Streptomyces scabies, Streptomyces lividans, Streptomyces turgidiscabies, Streptomyces murinus, and Streptomyces acidiscabies; in particular the Streptomyces strain is selected from the group consisting of Streptomyces scabies, Streptomyces acidiscabies, and Streptomyces turgidiscabies; especially the Streptomyces strain is a Streptomyces acidiscabies strain.

The compound of interest may be obtained from a gram negative bacterium , in particular from an Escherichia sp. strain, e.g., Escherichia coli, or from a Pseudomonas sp. strain.

Fungus

The fungus expressing the compound of interest according to the invention may be a fungus of any genus including yeast. In a preferred embodiment the fungus is a filamentous fungus.

According to the invention the fungus may especially be a filamentous fungal strain selected from the group consisting of Achlya, Acremonium, Aspergillus, Aureobasidium, Cephalosporium, Cochliobolus, Cryptococcus, Endothia, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Piromyces, Podospora, Pyricularia, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, and Trichoderma, in particular the fungus may be from the group consisting of Achlya, Aspergillus, Cephalosporium, Cochliobolus, Endothia, Fusarium, Humicola, Mucor, Neurospora, Penicillium, Podospora, Pyricularia, and Trichoderma.

In another preferred embodiment the fungus is an Aspergillus strain, in particular the fungus is selected from the group consisting of Aspergillus aculeatus, Aspergillus awamori, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, and Aspergillus oryzae.

In an especially preferred embodiment the fungus is a Trichoderma strain, particularly a Trichoderma reseeii strain.

Bacterial and fungal strains of these species are readily accessible to the public in a number of culture collections, such as the American Type Culture Collection (ATCC) , Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Centraalbureau Voor Schimmelcultures (CBS), and Agricultural Research Service Patent Culture Collection, Northern Regional Research Center (NRRL).

Compound of interest

The compound of interest according to the invention may be any valuable compound produced by a microorganism, in particular a secondary metabolite.

The compound may be a phytotoxin such as a thaxtomin. The compound of interest may also be a therapeutic protein, or an enzyme (e.g., a hydrolase, a transferase, a lyase, an isomerase, or a ligase; in particular a cellulase).

The compound of interest according to the invention may be an antibiotic such as penicillin or cephalosporin or erythromycin, or a commodity chemical such as citric acid.

Thaxtomin

A compound of interest according to the invention may be a thaxtomin. Thaxtomins are a known group of phytotoxins.

Thaxtomins include any of the type from a family of cyclic dipeptides, such as 4- nitroindol-3-yl-containing 2,5-dioxopiperazines. Suitable thaxtomins include agents described as cyclic dipeptides having the basic structure cyclo-(L-4-nitrotryptophyl-L-phenylalanyl). In embodiments, suitable diketopiperazine moieties may be /V-methylated, and include congeners carrying phenylalanyl alpha- and ring-carbon hydroxyl groups. Non-limiting examples of suitable thaxtomins for use in accordance with the present invention include thaxtomin A, thaxtomin A ortho isomer, thaxtomin B, and C-14 deoxythaxtomin B (thaxtomin D), and derivatives of any of these.

Thaxtomins include the following formula:

In embodiments, is methyl or H.

In embodiments, R₂ s hydroxy or H

In embodiments, R₃ is methyl or H.

In embodiments, R₄ is hydroxy or H

In embodiments, R₅ s hydroxy or H

In embodiments, R₆ is hydroxy or H

n embodiments, is methyl, R₂ is hydroxy, R₃ is methyl, R₄ is H, R₅ is hydroxy and R₆ is H.

n embodiments, is methyl, R₂ is hydroxy, R₃ is methyl, R₄ is hydroxy, R₅ is H and R₆ is H.

In embodiments, R^ is methyl, R₂ is H, R₃ is H, R₄ is H, R₅ is H and R₆ is H.

In embodiments, R^ is methyl, R₂ is hydroxy, R₃ is methyl, R₄ is H, R₅ is H and R₆ is

H.

In embodiments, R^ is methyl, R₂ is H, R₃ is methyl, R₄ is H, R₅ is H and R₆ is H.

In embodiments, R^ is methyl, R₂ is hydroxy, R₃ is H, R₄ is H, R₅ is H and R₆ is H. In embodiments, R^ is methyl, R₂ is hydroxy, R₃ is methyl, R₄ is H, R₅ is H and R₆ is hydroxy.

In embodiments, R^ is methyl, R₂ is hydroxy, R₃ is methyl, R₄ is H, R₅ is hydroxy and R₆ is hydroxy.

In embodiments, R^ is methyl, R₂ is hydroxy, R₃ is H, R₄ is H, R₅ is hydroxy and R₆ is

H.

In embodiments, R^ is H, R₂ is hydroxy, R₃ is methyl, R₄ is H, R₅ is hydroxy and R₆ is

H.

In embodiments, R^ is H, R₂ is H, R₃ is H, R₄ is H, R₅ is H and R₆ is H.

Thaxtomin A is composed of 4-nitroindol-3-yl-containing 2,5-dioxopiperazine and is the predominant thaxtomin produced by Streptomyces scabies, Streptomyces acidiscabies, and Streptomyces turgidiscabies, with phenylalanyl m-ring and alpha-C hydroxyl additions. The chemical composition comprises, or consists of:

Proteins

A compound of interest according to the invention may be a protein.

In a preferred embodiment, the protein of interest is an enzyme, in particular a hydrolase (class EC 3 according to Enzyme Nomenclature; Recommendations of the Nomenclature Committee of the International Union of Biochemistry).

In a particular preferred embodiment the following hydrolases are preferred: Amyloglucosidases: Amyloglucosidases (also called glucoamylases and glucan 1 ,4-alpha- glucosidase, EC 3.2.1.3) are enzymes which catalyze the release of D-glucose from the non- reducing ends of starch or related oligo- and polysaccharide molecules.

Suitable amyloglucosidases include those of fungal origin, especially those from filamentous fungi or yeasts, e.g., Talaromyces emersonii, Aspergillus niger and Aspergillus awamori. Chemically modified or protein engineered mutants are included.

An example of a useful Talaromyces emersonii amyloglucosidase is described in WO 99/28448. An example of a commercially available amyloglucosidase is AMG™ (Novozymes A/S).

Amylases: Suitable amylases (alpha and/or beta) include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Amylases include, for example, alpha-amylases obtained from Bacillus, e.g. a special strain of B. licheniformis, described in more detail in GB 1 ,296,839.

Examples of useful amylases are the variants described in WO 94/02597, WO 94/18314, WO 96/23873, WO 97/43424, and WO 01/66712, especially the variants with substitutions in one or more of the following positions: 15, 23, 105, 106, 124, 128, 133, 154, 156, 181 , 188, 190, 197, 202, 208, 209, 243, 264, 304, 305, 391 , 408, and 444.

Especially suitable amylases are the acid amylases of fungal origin, e.g. , acid amylase from Aspergillus niger.

Commercially available amylases are DURAMYL™, TERMAMYL™, FUNGAMYL™, NATALASE™, T E R M A MY L LC™, TERMAMYL SC™, LI QU IZYM E-X™ and BAN™ (Novozymes A/S), RAPIDASE™ and PURASTAR™ (from Genencor International Inc.).

Cellulases: Suitable cellulases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Suitable cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium, a n d Trichoderma, e.g., the fungal cellulases produced from Humicola insolens, Myceliophthora thermophila, Fusarium oxysporum and Trichoderma reesei; in particular cellulases from Trichoderma reesei are preferred; (examples are disclosed in US 4,435,307, US 5,648,263, US 5,691 ,178, US 5,776,757 and WO 89/09259).

Commercially available cellulases include CELLUCLAST™, CELLUZYME™, CAREZYME™, and CAREZYME CORE™ (Novozymes A/S), CLAZINASE™, and PURADAX HA™ (Genencor International Inc.), and KAC-500(B)™ (Kao Corporation). Lipases: Suitable lipases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Examples of useful lipases include lipases from

Humicola (synonym Thermomyces), e.g. from H. lanuginosa (T lanuginosus) as described in

EP 258 068 and EP 305 216 or from H. insolens as described in WO 96/13580, a Pseudomonas lipase, e.g. from P. alcaligenes or P. pseudoalcaligenes (EP 218 272), P. cepacia (EP 331 376), P. stutzeri (GB 1 ,372,034), P. fluoresceins, Pseudomonas sp. strain SD 705 (WO 95/06720 and WO 96/27002), P. wisconsinensis (WO 96/12012), a Bacillus lipase, e.g. from B. subtilis (Dartois et al. (1993), Biochemica et Biophysica Acta, 1 131 , 253- 360), B. stearothermophilus (JP 64/744992) or B. pumilus (WO 91/16422).

Other examples are lipase variants such as those described in WO 92/05249, WO 94/01541 , EP 407 225, EP 260 105, WO 95/35381 , WO 96/00292, WO 95/30744, WO 94/25578, WO 95/14783, WO 95/22615, WO 97/04079 and WO 97/07202.

Preferred commercially available lipase enzymes include LIPOLASE™, LIPOLASE ULTRA™ and LIPEX™ (Novozymes A/S).

Proteases: Suitable proteases include those of animal, vegetable or microbial origin. Microbial origin is preferred. Chemically modified or protein engineered mutants are included. The protease may be an acid protease, a serine protease or a metallo protease, preferably an alkaline microbial protease or a trypsin-like protease. Examples of alkaline proteases are subtilisins, especially those derived from Bacillus, e.g., subtilisin Novo, subtilisin Carlsberg, subtilisin 309, subtilisin 147 and subtilisin 168 (described in WO 89/06279). Examples of trypsin-like proteases are trypsin (e.g. of porcine or bovine origin) and the Fusarium protease described in WO 89/06270 and WO 94/25583.

Preferred commercially available protease enzymes include ALCALASE™,

SAVINASE™, PRIMASE™, DURALASE™, ESPERASE™, RELASE™ and KANNASE™ (Novozymes A/S), MAXATASE™, MAXACAL™, MAXAPEM™, PROPERASE™, PURAFECT™, PURAFECT OXP™, FN2™, and FN 3™ (Genencor International Inc.).

Other preferred hydrolases: Other preferred hydrolases are carbohydrolases including MANNAWAY™ (Novozymes A/S).

Other hydrolases such as transferases, lyases, isomerases, and ligases may also be produced according to the invention.

Modification of the microorganism of interest

The microorganism producing the compound of interest has typically been transformed with an expression construct comprising a promoter operably linked to a gene encoding the compound of interest. Various promoters are used and described in the prior art.

It is normally also beneficial to have more than one copy of the compound encoding the compound of interest in order to make the yield as high as possible.

The promoter is preferably an inducible promoter. Fermentations

The present invention may be useful for any fermentation in industrial scale, e.g., for any fermentation having culture media of at least 50 litres, preferably at least 100 litres, more preferably at least 500 litres, even more preferably at least 1000 litres, in particular at least 5000 litres.

The microorganism may be fermented by any method known in the art. The fermentation medium may be a complex medium comprising complex nitrogen and/or carbon sources, such as soybean meal, soy protein, soy protein hydrolysate, cotton seed meal, corn steep liquor, yeast extract, casein, casein hydrolysate, potato protein, potato protein hydrolysate, molasses, and the like. The fermentation medium may be a chemically defined media, e.g., as defined in WO 98/37179.

The fermentation may be performed as a batch, a fed-batch, a repeated fed-batch or a continuous fermentation process.

Carbon limited conditions

It may be an advantage according to the invention to use a carbon l im ited fermentation.

Carbon limited conditions mean that the microorganism has just enough carbon to grow with a specific growth rate wherein said specific growth rate is lower than the maximum specific growth rate. Browned glucose

The present invention discloses the surprisingly high advantage by adding a browned glucose solution to a culture medium instead of just adding a glucose solution to a culture medium.

The browned glucose solution is a glucose solution that has been acid treated and heated to a temperature of at least 90 degrees Celsius, and wherein the glucose solution has a concentration of at least 500 g/l.

In a preferred embodiment the glucose solution is first acid treated and then heated to a temperature of at least 90 degrees Celsius.

The glucose solution should have a high concentration of glucose. In a preferred embodiment the glucose solution has a concentration of least 500 g/l; preferably a concentration of at least 550 g/l; preferably a concentration of at least 600 g/l; preferably a concentration of at least 650 g/l; preferably a concentration of at least 700 g/l; preferably a concentration of at least 750 g/l; preferably a concentration of at least 800 g/l; preferably a concentration of at least 850 g/l; preferably a concentration of at least 900 g/l; preferably at least 950 g/l; preferably a concentration of at least 1000 g/l; preferably a concentration of at least 1050 g/l; preferably a concentration of at least 1 100 g/l; in particular the glucose solution has a concentration of from 500 g/l to 1200 g/l; especially the glucose solution has a concentration of from 600 g/l to 1200 g/l; especially the glucose solution has a concentration of from 700 g/l to 1200 g/l; and even more preferably the glucose solution has a concentration of from 800 g/l to 1200 g/l.

Any acid known in the art may be used for the acid treatment. A preferred acid is phosphoric acid, sulphuric acid, hydrochloric acid, nitric acid, or citric acid; in particular phosphoric acid or sulphuric acid.

The acid treatment results in a glucose solution - before the heat treatment - with a pH less than 4.5; in particular with a pH less than 4.0; in particular with a pH less than 3.5; in particular with a pH less than 3.0; in particular with a pH less than 2.5; in particular with a pH less than 2.0; in particular with a pH less than 1.5; in particular with a pH less than 1.0; especially the pH is in the range of from pH 0.5 to pH 4.5; and even more preferably the pH is in the range of from pH 0.5 to pH 3.5.

The acid treated glucose solution is heated to a temperature of at least 90 degrees Celsius; in particular to a temperature of at least 95 degrees Celsius; in particular to a temperature of at least 100 degrees Celsius; in particular to a temperature of at least 101 degrees Celsius; in particular to a temperature of at least 102 degrees Celsius; in particular to a temperature of at least 103 degrees Celsius; in particular to a temperature of at least 104 degrees Celsius; in particular to a temperature of at least 105 degrees Celsius; in particular to a temperature of at least 106 degrees Celsius; in particular to a temperature of at least 107 degrees Celsius; in particular to a temperature of at least 108 degrees Celsius; in particular to a temperature of at least 109 degrees Celsius; in particular to a temperature of at least 110 degrees Celsius; in particular to a temperature of at least 1 11 degrees Celsius; in particular to a temperature of at least 112 degrees Celsius; in particular to a temperature of at least 1 13 degrees Celsius; in particular to a temperature of at least 1 14 degrees Celsius; in particular to a temperature of at least 1 15 degrees Celsius; in particular to a temperature of at least 1 16 degrees Celsius; in particular to a temperature of at least 1 17 degrees Celsius; in particular to a temperature of at least 1 18 degrees Celsius; in particular to a temperature of at least 1 19 degrees Celsius; in particular to a temperature of at least 120 degrees Celsius; in particular to a temperature of at least 121 degrees Celsius; in particular to a temperature of at least 122 degrees Celsius; in particular to a temperature of at least 123 degrees Celsius; in particular to a temperature of at least 124 degrees Celsius; in particular to a temperature of at least 125 degrees Celsius; in particular to a temperature of at least 130 degrees Celsius; in particular to a temperature of at least 135 degrees Celsius; preferably to a temperature in the range of from 90 to 135 degrees Celsius; in particular to a temperature in the range of from 100 to 135 degrees Celsius; and even more preferably to a temperature in the range of from 110 to 130 degrees Celsius.

The high temperatures are typically obtained by use of an autoclave. Various types of autoclaves are known in the art.

Recovery of the compound of interest

The product according to the invention may be a crude product; e.g., the product may be directly obtained from the fermentation broth.

A further aspect of the invention concerns the downstream processing of the fermentation broth. After the fermentation process is ended, the compound of interest may be recovered from the fermentation broth, using standard technology developed for the compound of interest.

The relevant downstream processing technology to be applied depends on the nature of the compound of interest.

A process for the recovery of a compound of interest from a fermentation broth will typically (but is not limited to) involve one or more of the following steps:

1) pre-treatment of broth (e.g., pH treatment and/or flocculation)

2) removal of cells and other solid material from broth (primary separation)

3) filtration

4) concentration

5) filtration

6) stabilization and standardization.

Apart from the unit operations listed above, a number of other recovery procedures and steps may be applied, e.g., variation in temperature, crystallization, treatment of the solution comprising the compound of interest with active carbon, and use of various adsorbents.

The invention is further illustrated in the following example, which is not intended to be in any way limiting to the scope of the invention as claimed.

Example 1

Effect of browned glucose on the production of thaxtomin A from Streptomyces acidiscabies

The following example shows that the use of a browned glucose solution, instead of a glucose solution, results in a strong induction of the thaxtomin A production in Streptomyces acidiscabies.

Media:

2 g/L Difco yeast extract

0.025g/L CuS04 5H20 2.44 g/L Na2HP04 monohydrate

7.5 g/L K2HP04

2.5 g/L (NH4)2S04

2 g/L Citric Acid

2.5 g/L MgS04 7H20

0.25 g/L CaC03

3 g/L K2S04

100 mM MES buffer (20 g/l)

Premix (trace elements solution - see recipe below) 10 mL/L

pH ~6.0

500 ml glass Shake flasks with 2 baffels were used with 100 ml media.

0.1 ml P2000 antifoam was added to each flask. Premix Trace Elements solution Recipe

0.4 g/L ZnS04 7H20

0.2 g/L CuS04 5H20

1.5 g/L FeS04 7H20

0.6 g/L MnS04 H20

20 g/L Citric Acid

After a heat treatment (121 degrees Celsius; 20 min) the carbohydrate source was added to the shake flasks. Carbohydrate sources:

2.44 ml glucose solution

820 g/l glucose, 1 H20; heat treated (autoclaved) 1 hr. at 121 degrees Celsius Or

2.13 ml browned glucose solution - acid and heat treated in the following way:

940 g/l glucose, 1 H20

87.2 g/l 16% H3P04

0.6 g/l SB 2121 (antifoam)

Heat treated (autoclaved) for 1.5 hr. at 121 degrees Celsius

The shake flasks were inoculated with 1 ml of a strongly growing inoculum culture (see details below). Making inoculum for the cultivations:

The strain was inoculated from a cryo vial, and first grown on a PDA agar for 3 days at 28 degrees Celsius. A single colony was cut out of the agar and used for inoculation of a shake flask with the above described media and glucose as carbohydrate source. The shake flask was incubated for 2 days at 28 degrees Celsius 200 RPM; after 1 day the wall growth was shaken down into the media/culture by a short period of vigorous manual shaking. This culture was used for inoculums of the above described shake flasks. The growth and production of thaxtomin A was measured after 2 days of cultivation:

Conclusion:

Based on OD 650 which correlates well with the cell dry mass, the two carbon sources result in approximately the same level of biomass, while the level of thaxtomin A is surprisingly higher in the culture having browned glucose as the carbohydrate source.

The yield is 170 % compared to the culture having glucose as carbohydrate source.

Claims

I . A method for fermenting a microorganism, producing a compound of interest, in a culture medium comprising:

2. The method according to claim 1 , wherein the microorganism is a bacterium or a fungus.

3. The method according to claim 2, wherein the bacterium is selected from the group consisting of Bacillus, Streptomyces, Escherichia, and Pseudomonas.

4. The method according to claim 2, wherein the fungus is selected from the group consisting of Achlya, Aspergillus, Cephalosporium, Cochliobolus, Endothia, Fusarium, Humicola, Mucor, Neurospora, Penicillium, Podospora, Pyricularia, and Trichoderma.

5. The method according to claim 1 , wherein the compound of interest is a secondary metabolite.

6. The method according to claim 1 , wherein the compound of interest is a protein.

7. The method according to claim 6, wherein the protein is an enzyme.

8. The method according to claim 1 , wherein the fermentation is a batch, a fed batch, a repeated fed batch or a continuous fermentation.

9. The method according to claim 1 , wherein the acid treatment results in a glucose solution with a pH less than pH 4.5.

10. The method according to claim 1 , wherein the glucose solution is acid treated and thereafter heated to a temperature of at least 90 degrees Celsius.

I I . The method according to claim 1 , wherein the glucose solution has a concentration of from 500 g/l to 1200 g/l.

12. The method according to claim 1 , wherein the microorganism has been transformed with an expression construct comprising a promoter operably linked to a gene encoding the compound of interest.

13. The method according to claim 12, wherein the promoter is an inducible promoter.

14. The method according to claim 1 , wherein the compound of interest is recovered after removal of the microorganism.

The method according to claim 5, wherein the secondary metabolite is Thaxtomin.