WO2012103281A3 - Systems and methods for mitigation of inhibitors using yeast - Google Patents

Systems and methods for mitigation of inhibitors using yeast Download PDF

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Publication number
WO2012103281A3
WO2012103281A3 PCT/US2012/022645 US2012022645W WO2012103281A3 WO 2012103281 A3 WO2012103281 A3 WO 2012103281A3 US 2012022645 W US2012022645 W US 2012022645W WO 2012103281 A3 WO2012103281 A3 WO 2012103281A3
Authority
WO
WIPO (PCT)
Prior art keywords
hydrolysate
yeast
systems
methods
inhibitors
Prior art date
Application number
PCT/US2012/022645
Other languages
French (fr)
Other versions
WO2012103281A2 (en
Inventor
Jason KWIATKOWSKI
Neelakantam NARENDRANATH
Original Assignee
Poet Research, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Poet Research, Inc. filed Critical Poet Research, Inc.
Publication of WO2012103281A2 publication Critical patent/WO2012103281A2/en
Publication of WO2012103281A3 publication Critical patent/WO2012103281A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Systems and methods for mitigating fermentation inhibitors in a hydrolysate are disclosed. A system includes combining and mixing 200 grams active dry yeast per liter of hydrolysate in a reaction vessel. The mixture and hydrolysate are equilibrated at a defined pH, temperature, and timing in order to minimize the metabolism of sugars in the hydrolysate by the yeast. In some embodiments, the equilibration is maintained at about 25 C for 30 minutes. After equilibration, the yeast may be separated from the clean hydrolysate using a centrifuge or filter. The removed yeast cells may be recycled to the reaction vessel for subsequent treatments. The clean hydrolysate may be concentrated in order to yield concentrated hydrolysate.
PCT/US2012/022645 2011-01-27 2012-01-26 Systems and methods for mitigation of inhibitors using yeast WO2012103281A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161436736P 2011-01-27 2011-01-27
US61/436,736 2011-01-27

Publications (2)

Publication Number Publication Date
WO2012103281A2 WO2012103281A2 (en) 2012-08-02
WO2012103281A3 true WO2012103281A3 (en) 2012-11-15

Family

ID=46581395

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/022645 WO2012103281A2 (en) 2011-01-27 2012-01-26 Systems and methods for mitigation of inhibitors using yeast

Country Status (1)

Country Link
WO (1) WO2012103281A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
WO2011116320A1 (en) 2010-03-19 2011-09-22 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
EP2547778B1 (en) 2010-03-19 2019-09-04 POET Research, Inc. System for the treatment of biomass
US9469859B1 (en) 2010-08-12 2016-10-18 Poet Research, Inc. Method for treatment of biomass
CA2824993C (en) 2011-01-18 2019-07-23 Poet Research, Inc. Systems and methods for hydrolysis of biomass
WO2013006856A1 (en) 2011-07-07 2013-01-10 Poet Research Incorporated Systems and methods for acid recycle
US9963823B2 (en) 2015-05-13 2018-05-08 Poet Research, Inc. Methods of reducing the size of lignocellulosic material, and related systems
US10618850B2 (en) 2015-10-15 2020-04-14 Poet Research, Inc. Methods of extracting inorganic nutrients from pretreated biomass to form a fertilizer composition, and related systems
BR112018073839A2 (en) 2016-05-20 2019-02-26 Poet Res Inc methods for removing one or more compounds from a lignocellulosic hydrolyzate via gas drag and related systems
US10920247B2 (en) 2017-09-05 2021-02-16 Poet Research, Inc. Methods and systems for propagation of a microorganism using a pulp mill and/or a paper mill waste by-product, and related methods and systems
US11371012B2 (en) 2017-11-16 2022-06-28 Poet Research, Inc. Methods for propagating microorganisms for fermentation and related methods and systems
EP3724343A1 (en) 2017-12-14 2020-10-21 POET Research, Inc. Method for propagating microorganisms on a medium comprising stillage

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009030713A1 (en) * 2007-09-03 2009-03-12 Novozymes A/S Detoxifying and recycling of washing solution used in pretreatment of lignocellulose-containing materials
US20100233771A1 (en) * 2009-03-03 2010-09-16 Mcdonald William F System for pre-treatment of biomass for the production of ethanol
WO2011080130A2 (en) * 2009-12-21 2011-07-07 Sekab E-Technology Ab In situ detoxification of fermentation inhibitors with reducing agents

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009030713A1 (en) * 2007-09-03 2009-03-12 Novozymes A/S Detoxifying and recycling of washing solution used in pretreatment of lignocellulose-containing materials
US20100233771A1 (en) * 2009-03-03 2010-09-16 Mcdonald William F System for pre-treatment of biomass for the production of ethanol
WO2011080130A2 (en) * 2009-12-21 2011-07-07 Sekab E-Technology Ab In situ detoxification of fermentation inhibitors with reducing agents

Also Published As

Publication number Publication date
WO2012103281A2 (en) 2012-08-02

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