WO2012093149A3 - Nouvelles enzymes de déconstruction des parois cellulaires et leurs utilisations - Google Patents

Nouvelles enzymes de déconstruction des parois cellulaires et leurs utilisations Download PDF

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Publication number
WO2012093149A3
WO2012093149A3 PCT/EP2012/050130 EP2012050130W WO2012093149A3 WO 2012093149 A3 WO2012093149 A3 WO 2012093149A3 EP 2012050130 W EP2012050130 W EP 2012050130W WO 2012093149 A3 WO2012093149 A3 WO 2012093149A3
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WIPO (PCT)
Prior art keywords
seq
nos
set forth
nucleic acids
polypeptide
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PCT/EP2012/050130
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English (en)
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WO2012093149A2 (fr
Inventor
Adrian Tsang
Justin Powlowski
Gregory Butler
Reginald Storms
Original Assignee
Dsm Ip Assets B.V.
Valorbec Societe En Commandite
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Application filed by Dsm Ip Assets B.V., Valorbec Societe En Commandite filed Critical Dsm Ip Assets B.V.
Publication of WO2012093149A2 publication Critical patent/WO2012093149A2/fr
Publication of WO2012093149A3 publication Critical patent/WO2012093149A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne un nouveau procédé pour dégrader une biomasse ou une biomasse prétraitée en sucres faisant appel à une enzyme comprenant un polypeptide ayant a) une séquence polypeptidique telle que définie dans l'une quelconque des SEQ ID Nos : 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66 et 69; b) un polypeptide qui présente une homologie d'au moins 80 %, de préférence, d'au moins 85 %, plus préférablement, d'au moins 90 %, mieux encore, d'au moins 95, 96, 97, 98 ou 99 % avec l'une quelconque des SEQ ID Nos : 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66 et 69; c) une séquence polypeptidique codée par une séquence d'acides nucléiques telle que définie dans l'une quelconque des SEQ ID Nos : 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 et 68, ou des acides nucléiques présentant une homologie d'au moins 80 %, de préférence, d'au moins 85 %, plus préférablement, d'au moins 90 %, mieux encore, d'au moins 95, 96, 97, 98 ou 99 % avec l'une quelconque des SEQ ID Nos : 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 et 68; d) une séquence polypeptidique codée par une séquence d'acides nucléiques s'hybridant dans des conditions stringentes au polynucléotide tel que défini dans l'une quelconque des SEQ ID Nos : 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 et 68; ou e) une séquence polypeptidique codée par une séquence d'acides nucléiques s'hybridant dans des conditions stringentes au complément antisens d'un polynucléotide tel que défini dans l'une quelconque des SEQ ID Nos : 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 et 68.
PCT/EP2012/050130 2011-01-06 2012-01-05 Nouvelles enzymes de déconstruction des parois cellulaires et leurs utilisations WO2012093149A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161430533P 2011-01-06 2011-01-06
US61/430,533 2011-01-06

Publications (2)

Publication Number Publication Date
WO2012093149A2 WO2012093149A2 (fr) 2012-07-12
WO2012093149A3 true WO2012093149A3 (fr) 2013-08-22

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111100889B (zh) * 2018-10-26 2021-08-31 中国石油化工股份有限公司 一种提高木质纤维素酶解效率的方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014044708A1 (fr) * 2012-09-19 2014-03-27 Novozymes A/S Polypeptides à activité favorisant l'activité cellulolytique et polynucléotides codant pour ces polypeptides
CN104342424B (zh) * 2013-07-29 2017-12-01 瑞辰星生物技术（广州）有限公司 用于改变和改善纤维性质的纤维氧化酶组合物及造纸方法和应用
EP3074513A1 (fr) 2013-11-26 2016-10-05 Novozymes A/S Compositions enzymatiques et leurs utilisations
EP3152315B1 (fr) 2014-06-06 2018-08-15 Novozymes A/S Compositions d'enzymes et leur utilisations
BR112017019331A2 (pt) 2015-03-12 2018-06-05 Beta Renewables Spa processos para sacarificação de múltiplos estágios de um material lignocelulósico e para melhorar um rendimento de glicose ou xilose de sacarificação de um material lignocelulósico em um reator de tanque continuamente agitado
BR112017018461A2 (pt) 2015-03-12 2018-04-17 Novozymes As processos para hidrólise multiestágio, para produção de um produto de fermentação, e, para aumento de rendimento de açúcar de hidrólise.
TN2017000318A1 (en) 2015-03-12 2019-01-16 Beta Renewable Spa Multi-stage enzymatic hydrolysis of lignocellulosic biomass

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111100889B (zh) * 2018-10-26 2021-08-31 中国石油化工股份有限公司 一种提高木质纤维素酶解效率的方法

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