WO2012080541A8 - Novel reverse transcriptases of human immunodeficiency virus type 1 group o - Google Patents

Novel reverse transcriptases of human immunodeficiency virus type 1 group o Download PDF

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Publication number
WO2012080541A8
WO2012080541A8 PCT/ES2011/070801 ES2011070801W WO2012080541A8 WO 2012080541 A8 WO2012080541 A8 WO 2012080541A8 ES 2011070801 W ES2011070801 W ES 2011070801W WO 2012080541 A8 WO2012080541 A8 WO 2012080541A8
Authority
WO
WIPO (PCT)
Prior art keywords
reverse transcriptases
group
immunodeficiency virus
human immunodeficiency
virus type
Prior art date
Application number
PCT/ES2011/070801
Other languages
Spanish (es)
French (fr)
Other versions
WO2012080541A1 (en
Inventor
Luis MENÉNDEZ ARIAS
Verónica BARRIOLUENGO FERNÁNDEZ
María del Mar ÁLVAREZ GARCÍA
Original Assignee
Consejo Superior De Investigaciones Científicas (Csic)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior De Investigaciones Científicas (Csic) filed Critical Consejo Superior De Investigaciones Científicas (Csic)
Publication of WO2012080541A1 publication Critical patent/WO2012080541A1/en
Publication of WO2012080541A8 publication Critical patent/WO2012080541A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to reverse transcriptases isolated from human immunodeficiency virus type 1 (HIV-1), group O, and altered at position 65 and/or position 75, which have a high copying fidelity and thermostability, as well as to use thereof for performing the reverse transcription, amplification or sequencing of a template nucleic acid. The invention also relates to a method for obtaining said reverse transcriptases. The invention further relates to a reverse transcription method, an amplification method, a sequencing method using the reverse transcriptases of the invention and to a kit comprising the elements necessary for carrying out said methods.
PCT/ES2011/070801 2010-12-16 2011-11-21 Novel reverse transcriptases of human immunodeficiency virus type 2 group o WO2012080541A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES201031864A ES2386264B1 (en) 2010-12-16 2010-12-16 NEW HUMAN IMMUNODEFICIENCY VIRUS RETROTRANSCRIPTASAS TYPE 1 GROUP 0
ESP201031864 2010-12-16

Publications (2)

Publication Number Publication Date
WO2012080541A1 WO2012080541A1 (en) 2012-06-21
WO2012080541A8 true WO2012080541A8 (en) 2013-03-21

Family

ID=46244147

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2011/070801 WO2012080541A1 (en) 2010-12-16 2011-11-21 Novel reverse transcriptases of human immunodeficiency virus type 2 group o

Country Status (2)

Country Link
ES (1) ES2386264B1 (en)
WO (1) WO2012080541A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2525135B1 (en) 2013-05-17 2015-09-29 Consejo Superior De Investigaciones Científicas (Csic) HIV RETROTRANSCRIPTASAS TYPE 1 GROUP OR, ACTIVE AT HIGH TEMPERATURES
JP6769869B2 (en) 2013-10-16 2020-10-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド Reverse transcriptase with enhanced properties

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2358824B1 (en) * 2009-05-13 2012-03-21 Consejo Superior De Investigaciones Cient�?Ficas (Csic) GROUP OR MODIFIED HIV-1 RETROTRANSCRIPTASE.

Also Published As

Publication number Publication date
ES2386264B1 (en) 2013-07-10
ES2386264A1 (en) 2012-08-14
WO2012080541A1 (en) 2012-06-21

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