WO2012077891A1 - Patch permettant de régénérer un tissu endommagé et conçu avec un support fibreux poreux tridimensionnel - Google Patents

Patch permettant de régénérer un tissu endommagé et conçu avec un support fibreux poreux tridimensionnel Download PDF

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Publication number
WO2012077891A1
WO2012077891A1 PCT/KR2011/006463 KR2011006463W WO2012077891A1 WO 2012077891 A1 WO2012077891 A1 WO 2012077891A1 KR 2011006463 W KR2011006463 W KR 2011006463W WO 2012077891 A1 WO2012077891 A1 WO 2012077891A1
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WO
WIPO (PCT)
Prior art keywords
patch
damaged tissue
cells
tissue regeneration
support
Prior art date
Application number
PCT/KR2011/006463
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English (en)
Korean (ko)
Inventor
이승진
심인경
양영일
장양수
정미라
정혜진
Original Assignee
이화여자대학교 산학협력단
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Application filed by 이화여자대학교 산학협력단 filed Critical 이화여자대학교 산학협력단
Publication of WO2012077891A1 publication Critical patent/WO2012077891A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/58—Materials at least partially resorbable by the body
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Definitions

  • MI myocardial infarction
  • 7 (a) and 7 (b) are micrographs obtained by H & E staining of tissues after 4 days of implanting a patch seeded with cardiac stem cells on the outer pericardial surface of normal heart tissues according to Experimental Example 4;
  • the cells for regeneration of the damaged tissue seeded in the support are stem cells.
  • a patch in the form of a network having a three-dimensional structure may be manufactured to have an orientation. Because the heart muscle is directional and has to withstand pressure, it is replaced by a collector of a cylindrical drum instead of a collector of a normal stainless steel plate and the rotational speed is reduced.
  • a patch in the form of a fibrous network having a thickness of about 300 ⁇ was prepared, and then the pores were enlarged by physical expansion to prepare a patch using a fibrous porous three-dimensional support having a thickness of 1 mm (FIG. 1).
  • FIG. 8 shows a photograph immediately after implanting the patch seeded with the cardiac stem cells of Example 1 in the myocardial infarction portion, and (b) shows a tissue photograph after 14 days.
  • (c) shows a photograph immediately after implanting the patch without seeding the stem cells of Comparative Example 1 in the myocardial infarction portion and (d) shows the histological image after 14 days.
  • tissue transplanted with the cardiac stem cell seeded patch was stained with myocardial antibody and stained with a fluorescein isothiocyanate-conjugated secondary antibody. .
  • These represent a-sarcomer ic actinin (aSA) and troponin-T (TnT) cardiomyocyte markers, which are sarcomere proteins involved in cardiomyocyte contraction.
  • Example 1 A disease model was prepared in the same manner as in Example 1, except that the patches of Example 1 and Comparative Example 1 were regenerated 28 days after implantation into the anterior wall of the left ventricle.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Transplantation (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un patch permettant de régénérer un tissu endommagé et conçu avec un support fibreux poreux tridimensionnel constitué d'un matériau polymère biodégradable, qui comporte des cellules ou un médicament (pour régénérer un tissu endommagé) gainés dans un support et qui est susceptible d'être fixé directement sur l'organe dans lequel le tissu endommagé est présent. Selon la présente invention, le patch permettant de régénérer un tissu endommagé est facile à manipuler et présente une faculté de survie élevée des cellules après transplantation, de façon à assurer l'efficacité de traitement d'un agent de traitement des cellules vers une région malade exigeant une transplantation cellulaire. Ainsi, lorsque le support poreux tridimensionnel d'un matériau polymère biodégradable, qui comporte des cellules ou un médicament (pour régénérer un tissu endommagé) gainés dans un support, est fixé directement à un organe dans lequel le tissu endommagé est présent, le taux de réussite de transfert cellulaire peut être augmenté et les cellules peuvent se déplacer vers les zones ischémiques pour augmenter la production capillaire du tissu endommagé et ainsi accélérer la régénération du tissu.
PCT/KR2011/006463 2010-12-08 2011-08-31 Patch permettant de régénérer un tissu endommagé et conçu avec un support fibreux poreux tridimensionnel WO2012077891A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100124657A KR101226629B1 (ko) 2010-12-08 2010-12-08 섬유형 다공성 삼차원 지지체를 포함하여 구성되는 손상된 조직 재생용 패치
KR10-2010-0124657 2010-12-08

Publications (1)

Publication Number Publication Date
WO2012077891A1 true WO2012077891A1 (fr) 2012-06-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/006463 WO2012077891A1 (fr) 2010-12-08 2011-08-31 Patch permettant de régénérer un tissu endommagé et conçu avec un support fibreux poreux tridimensionnel

Country Status (2)

Country Link
KR (1) KR101226629B1 (fr)
WO (1) WO2012077891A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018105980A1 (fr) * 2016-12-07 2018-06-14 한국생산기술연구원 Support tridimensionnel rempli de fibres et son procédé de fabrication
CN108159491A (zh) * 2018-01-02 2018-06-15 山东百多安医疗器械有限公司 一种心肌干细胞种子及输送方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101746155B1 (ko) * 2015-08-17 2017-06-13 한국과학기술연구원 3차원 세포집합체 및 안지오포이에틴을 포함하는 허혈성 질환 예방 및 치료용 약학적 조성물
KR101915367B1 (ko) 2016-12-20 2018-11-05 인제대학교 산학협력단 심장줄기세포의 다층세포시트 및 이의 제조방법
EP4101440A4 (fr) * 2020-02-04 2024-02-21 Hierabio Inc. Composition pour injection intra-péricardique comprenant des cellules souches et son utilisation

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100571478B1 (ko) * 2003-10-28 2006-04-17 이승진 생분해성 고분자로 이루어진 섬유형 다공성 지지체 및그의 제조방법
KR100875189B1 (ko) * 2005-08-26 2008-12-19 이화여자대학교 산학협력단 전기방사를 이용한 조직 재생용 섬유형 삼차원 다공성 지지체 및 그의 제조방법
KR20100059367A (ko) * 2008-11-26 2010-06-04 한국과학기술연구원 인 시튜 조직재생용 지능형 다공성 생분해 고분자 지지체 및 이의 제조방법
KR20100106905A (ko) * 2009-03-24 2010-10-04 한국과학기술연구원 줄기세포를 혈관세포로 분화시키는 방법 및 이를 이용한 생체 내 혈관신생 유도

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100571478B1 (ko) * 2003-10-28 2006-04-17 이승진 생분해성 고분자로 이루어진 섬유형 다공성 지지체 및그의 제조방법
KR100875189B1 (ko) * 2005-08-26 2008-12-19 이화여자대학교 산학협력단 전기방사를 이용한 조직 재생용 섬유형 삼차원 다공성 지지체 및 그의 제조방법
KR20100059367A (ko) * 2008-11-26 2010-06-04 한국과학기술연구원 인 시튜 조직재생용 지능형 다공성 생분해 고분자 지지체 및 이의 제조방법
KR20100106905A (ko) * 2009-03-24 2010-10-04 한국과학기술연구원 줄기세포를 혈관세포로 분화시키는 방법 및 이를 이용한 생체 내 혈관신생 유도

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018105980A1 (fr) * 2016-12-07 2018-06-14 한국생산기술연구원 Support tridimensionnel rempli de fibres et son procédé de fabrication
CN108159491A (zh) * 2018-01-02 2018-06-15 山东百多安医疗器械有限公司 一种心肌干细胞种子及输送方法

Also Published As

Publication number Publication date
KR20120063619A (ko) 2012-06-18
KR101226629B1 (ko) 2013-01-28

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