WO2012068650A1 - Tranchée fluidodynamique destinée à des bioréacteurs à circuit horizontal avec impulsion par bulles - Google Patents

Tranchée fluidodynamique destinée à des bioréacteurs à circuit horizontal avec impulsion par bulles Download PDF

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Publication number
WO2012068650A1
WO2012068650A1 PCT/BR2010/000389 BR2010000389W WO2012068650A1 WO 2012068650 A1 WO2012068650 A1 WO 2012068650A1 BR 2010000389 W BR2010000389 W BR 2010000389W WO 2012068650 A1 WO2012068650 A1 WO 2012068650A1
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WO
WIPO (PCT)
Prior art keywords
pit
fluid
partition wall
vertical partition
tank
Prior art date
Application number
PCT/BR2010/000389
Other languages
English (en)
Portuguese (pt)
Inventor
Marcelo Rocha Martinelli
Daniel Fonseca De Carvalho E Silva
Leonardo Brantes Bacellar Mendes
Original Assignee
Petróleo Brasileiro S.A. - Petrobras
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Petróleo Brasileiro S.A. - Petrobras filed Critical Petróleo Brasileiro S.A. - Petrobras
Priority to PCT/BR2010/000389 priority Critical patent/WO2012068650A1/fr
Publication of WO2012068650A1 publication Critical patent/WO2012068650A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges

Definitions

  • the present invention relates to an impulse pit applied to bioreactor tanks, specifically of industrial scale microalgae production, which causes the culture medium to circulate in horizontal tanks, but capable of drastically reducing the energy consumption required at each time. productive cycle.
  • the system minimizes energy dispersion during the passage of fluid in the impulse pit.
  • Biodiesel is a natural fuel used in diesel cycle engines, produced from renewable oil sources, that meets the specifications of the National Petroleum Agency (ANP). It can be produced based on vegetable oils or animal fat.
  • biodiesel can also be added to petroleum derived fuel, forming a technically and economically viable blend. Mixing is possible and commercially permitted in any proportion with ultra-low sulfur diesel oil, giving it better lubricity characteristics.
  • Biodiesel requires large volumes of oilseeds for its production. With current seed processing techniques it is possible to extract on average 80% flour and only 20% oil. Thus, interest in the search for another plant source with promising oilseed characteristics increased: microalgae.
  • Microalgae have similar physicochemical and chemical characteristics to oilseed plants such as castor beans and soybeans, but unlike these have the advantage that they are not currently considered as the focus of human food. Other advantages reinforce the interest in microalgae: they, similar to oilseeds, require a very small space for cultivation, harvesting and transport costs are relatively low and there is a possibility of extracting more oil than oilseeds in general.
  • Microalgae cultivation has undeniable advantages: the multiplication rate of the crop is extraordinary, as it has a very short residence time per cycle of cultivated area. Yield is 200 to 300 times higher than known oilseeds. Its production area is 100 times smaller than traditional crops, requiring only 2,500 hectares to supply a refinery with a capacity of 250,000 tons, against 500,000 hectares of soybeans and 250,000 hectares of sunflower.
  • the micro-algae have the further advantage of "sequestering" carbon dioxide (C0 2) in its development process, thus contributing to the reduction of emissions of greenhouse gases to the atmosphere.
  • C0 2 carbon dioxide
  • Today's photobioreactors feature varied construction techniques and configurations for optimal use of sunlight exposure as well as agitation of the culture fluid.
  • Most adopted methods for cultivation of microalgae on an industrial scale focused on energy sources, culture in large open or closed tanks is predominant.
  • They are generally shaped like a masonry structure in shallow, divided oval shape to form at least two parallel channels, at least one of which is provided with at least one stirrer for moving the suspended biomass.
  • Agitation is generally promoted by submerged pumping, air injection or alternatively by rotating blades.
  • the pond volume is estimated according to the following parameters: hydraulic retention time (minimum re 30 days); applied organic load (Color, maximum 2000 mg / L) and movement speed, standardized around 0.15 m / s for the optimization of algae production.
  • the circulation and agitation of the culture fluid inside the tank are important to the good performance of the photosynthetic system, as it moves the suspended biomass, allowing a homogenization of microalgae to sunlight exposure and nutrients.
  • the configuration of a standard impulse pit currently used to promote circulation in microalgae growing tanks, consists of a parallelepiped shaped pit provided below the bottom of one of the growing tank channels.
  • the gap has the same width as said channel; It is divided by a vertical wall, transversely to the direction of flow of the fluid, resulting in two adjacent columns and also in the form of parallelepiped.
  • Each of these two columns connects at the top with the tank channel and at the bottom with each other, as the dividing wall does not reach the bottom of the pit.
  • a quantity of gas (which may be air or other gas of interest) is injected by bubblers. This gas, when rising through the fluid causes circulation in the system.
  • the fluid from the tank enters the pit downward in the upstream part of the tank channel, passes under the dividing wall of the pit and rises with the gas downstream of the channel.
  • Each edge of the pit inlet and outlet columns, as well as the lower edges at the intersection of the columns, also represent pressure drop zones.
  • an impeller pit always provided with a linear width equivalent to the width of the tank circulation channel, it will therefore have four times the same linear extension as a zone capable of generating pressure loss, since each of the four edges represents a zone of sudden change of direction. and layer breakage boundaries of the flow.
  • the pressure drop to promote the circulation of the entire culture fluid volume along the length of the culture tank channels represents 10% to 20% of the total system absorbed relative to the remaining energy expenditure that occurs only within of the impeller gap.
  • the present invention aims to offer a new stirring and thrusting option for industrial scale bioreactors of simple technical application which allows for greater economic viability per cultivation cycle.
  • the present invention relates to an agitator and impeller device, more specifically a fluid dynamic ditch, applied to bubbling horizontal circuit bioreactors, which comprises a basic structure composed of a predominantly parallelepiped shaped configuration tank having a curvilinear finish; arranged from edge to edge of at least one of the channels of a tank.
  • the pit is provided with a vertical partition wall, transversely to the direction of flow of the fluid but not reaching the bottom of said fossa.
  • the wall divides the pit so that two contiguous columns result, and also predominantly cobblestone. Each of these two columns connects at the top with the tank channel and at the bottom end with each other.
  • R1 - corresponding to the radius of curvature between the tank bed and the internal inlet and outlet walls of the pit;
  • R2 corresponds to the radius of curvature between the base of the pit and the inner walls of the inlet and outlet of the pit;
  • R3 - corresponding to the radius of curvature between the vertical partition wall and the fluid surface
  • R4 - corresponding to the radius of curvature of the vertical partition wall.
  • Figure 1 depicts a schematic view of a microalgae culture tank provided with a fluid dynamic ditch as proposed.
  • Figure 2 presents an isometric perspective view of the preferred constructive configuration of said fluid dynamic ditch, applied to bubbling horizontal loop photobioreactors.
  • Figure 3 depicts a cross-sectional view of said fluid-dynamic moat applied to bubbling horizontal loop bioreactors, indicating the relevant parameters.
  • the fluid dynamic ditch applied to bubbling horizontal loop bioreactors is intended to reduce the energy consumption expended during biomass revolving and cultivation fluid thrust within a photobioreactor.
  • the purpose of microalgae cultivation is for energy production, it is essential to detect and eliminate any waste of energy related to the process, so that the final energy balance is as profitable as possible.
  • Figure 1 shows a schematic view of a tank (1) where the thrust is made from a vertical bubbling moat.
  • the image already depicts the fluid dynamic ditch 100 proposed by the present invention.
  • Figure 2 shows in detail a schematic cross-sectional view of the fluid dynamic ditch (100) applied to bubbling horizontal loop bioreactors, which minimizes the problems highlighted above.
  • the invention basically comprises a predominantly parallelepiped configuration (110) disposed from one side to the other of one of the channels (2) of a microalgae cultivation tank (1), so that the upper end of the fluid dynamic ditch (100) is provided at the same level as the bed of said channel.
  • a predominantly parallelepiped configuration (110) disposed from one side to the other of one of the channels (2) of a microalgae cultivation tank (1), so that the upper end of the fluid dynamic ditch (100) is provided at the same level as the bed of said channel.
  • Alternatively, in the same tank (1) and / or channel (2), as many fluid-dynamic pits (100) as the design requirements may require to maintain optimal flow may be provided.
  • the fluid dynamic dome 100 has a vertical dividing wall 120 transverse to the direction of flow of the fluid. Said wall divides the pit 110 so that two adjacent columns result, a first column 121 and a second column 122, also predominantly parallelepiped in shape.
  • Each of these two columns (121 and 122) connects at the top with the channel (2) of the tank (1), and at the bottom end with each other, as the vertical dividing wall (120) does not reach the bottom of the pit (110).
  • gas is injected by means of bubbles (not shown in the figure). This gas, when rising through the fluid towards the surface, causes circulation in the system.
  • the fluid from the tank (1) enters the sump (110) downwards through the upper end of the first column (121) upstream of the channel (2 ') of the tank (1), it passes under the vertical partition wall (120) of the sump (110) and rises with the gas thrust through the second column (122) to the downstream portion of the channel (2 ").
  • the fluid dynamic pit 100 provides the entry of the first column 121 upstream of the channel. (2 '), the second column outlet (122) to the downstream portion of the channel (2 ") and also the lower end of the vertical partition wall (120), with a curvilinear finish. obeys pre-computed equational parameters by computational modeling that result in a constructive configuration capable of shaping the flow of culture fluid to minimize the breakdown of boundary layers, bringing the flow closer to a laminar regime during its passage through the interior. of the fluid dynamic ditch (100).
  • the fluid-dynamic moat 100 applied to bubbling horizontal loop bioreactors, may employ a much smaller flow gas disperser than those currently used.
  • Figure 3 shows a cross-sectional image of the constructive configuration of the fluid dynamic ditch 100, in which the following key variables can be identified in order for the invention to actually achieve the desired economy.
  • D2 distance between the lower end of the vertical partition wall (120) and the bottom of the pit (110);
  • R1 radius of curvature between the tank bed (1) and the internal inlet and outlet walls of the pit (110);
  • R2 radius of curvature between the base of the pit (110) and the inner walls of the inlet and outlet of the pit (110);
  • R3 radius of curvature between the vertical partition wall (120) and the fluid surface
  • R4 radius of curvature of the vertical partition wall (120).
  • the configuration variables disclosed above should preferably be restricted to a range of values that meet specific criteria.
  • a factor value, corresponding to one of the variables, must be chosen within the range of maximum and minimum possible values for that parameter, and multiplied by the square root of the intended floor area value (Au), expressed in square meters. .
  • the result of the operation will be the dimensional value to be used for the chosen configuration variable, expressed in decimeters:
  • the dimensional value of the other variables is obtained by the same methodology, applying the chosen factors in the formula. directly in the same factor table, in the other maximum and minimum value ranges of the remaining variables.
  • a random factor value within the factor range of each variable can be chosen that will achieve significant savings in the load losses generated by the bubbling drive system.

Abstract

La présente invention concerne un dispositif d'agitation/impulsion, et notamment une tranchée fluidodynamique destinée à des bioréacteurs à circuit horizontal avec impulsion par bulles, comprenant une structure de base permettant de réduire les dispersions d'énergie pendant le passage du fluide dans la tranchée d'impulsion. La tranchée d'impulsion trouve une application dans des réservoirs à bioréacteurs, et notamment dans les réservoirs de production de micro-algues à l'échelle industrielle, et permet de réduire considérablement la consommation d'énergie nécessaire à chaque cycle de production.
PCT/BR2010/000389 2010-11-25 2010-11-25 Tranchée fluidodynamique destinée à des bioréacteurs à circuit horizontal avec impulsion par bulles WO2012068650A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/BR2010/000389 WO2012068650A1 (fr) 2010-11-25 2010-11-25 Tranchée fluidodynamique destinée à des bioréacteurs à circuit horizontal avec impulsion par bulles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/BR2010/000389 WO2012068650A1 (fr) 2010-11-25 2010-11-25 Tranchée fluidodynamique destinée à des bioréacteurs à circuit horizontal avec impulsion par bulles

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WO2012068650A1 true WO2012068650A1 (fr) 2012-05-31

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018210790A1 (fr) * 2017-05-15 2018-11-22 Sustainwater S.À.R.L. Module de régulation et/ou de traitement des flux de liquide dans un canal de circulation dudit liquide, installation incorporant un tel module et procédé de mise en œuvre

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3468057A (en) * 1966-06-01 1969-09-23 Inst Francais Du Petrole Process for the culture of algae and apparatus therefor
US4217728A (en) * 1977-10-11 1980-08-19 Dainippon Ink & Chemicals Inc. Apparatus for cultivating algae
JPS62220183A (ja) * 1986-03-20 1987-09-28 Toshiro Sekine 微細藻類培養装置
US6037170A (en) * 1996-12-30 2000-03-14 Sekine; Toshirou Apparatus for culturing microalgae
BRPI0505266A (pt) * 2005-11-14 2007-08-07 Int Inst Nac De Tecnologia um novo processo de agitação de culturas de microalgas em tanques usando bombeador por borbulhamento
US20080311646A1 (en) * 2005-12-12 2008-12-18 Institute Of Process Engineering, Chinese Academy Of Sciences Carbon Supply Device for Cultivating Miro Algae in Large and Its Application Method and Use
BRPI0703633A2 (pt) * 2007-09-19 2009-05-12 Petroleo Brasileiro Sa sistema de agitação de fluxo vertical e baixo consumo de energia aplicado em fotobiorreatores para produção industrial de micro algas
WO2010012028A1 (fr) * 2008-07-29 2010-02-04 Bioseq Pty Ltd Photobioréacteur immergé couvert pour culture intensive de micro-algues à grande échelle

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3468057A (en) * 1966-06-01 1969-09-23 Inst Francais Du Petrole Process for the culture of algae and apparatus therefor
US4217728A (en) * 1977-10-11 1980-08-19 Dainippon Ink & Chemicals Inc. Apparatus for cultivating algae
JPS62220183A (ja) * 1986-03-20 1987-09-28 Toshiro Sekine 微細藻類培養装置
US6037170A (en) * 1996-12-30 2000-03-14 Sekine; Toshirou Apparatus for culturing microalgae
BRPI0505266A (pt) * 2005-11-14 2007-08-07 Int Inst Nac De Tecnologia um novo processo de agitação de culturas de microalgas em tanques usando bombeador por borbulhamento
US20080311646A1 (en) * 2005-12-12 2008-12-18 Institute Of Process Engineering, Chinese Academy Of Sciences Carbon Supply Device for Cultivating Miro Algae in Large and Its Application Method and Use
BRPI0703633A2 (pt) * 2007-09-19 2009-05-12 Petroleo Brasileiro Sa sistema de agitação de fluxo vertical e baixo consumo de energia aplicado em fotobiorreatores para produção industrial de micro algas
WO2010012028A1 (fr) * 2008-07-29 2010-02-04 Bioseq Pty Ltd Photobioréacteur immergé couvert pour culture intensive de micro-algues à grande échelle

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018210790A1 (fr) * 2017-05-15 2018-11-22 Sustainwater S.À.R.L. Module de régulation et/ou de traitement des flux de liquide dans un canal de circulation dudit liquide, installation incorporant un tel module et procédé de mise en œuvre
LU100201B1 (fr) * 2017-05-15 2018-11-26 Sustainwater S A R L Module de régulation et/ou de traitement des flux de liquide dans un canal de circulation dudit liquide, installation incorporant un tel module et procédé de mise en oeuvre

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