WO2012054391A2 - Compositions et procédés pour réduire les niveaux microbiens sur une surface - Google Patents

Compositions et procédés pour réduire les niveaux microbiens sur une surface Download PDF

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WO2012054391A2
WO2012054391A2 PCT/US2011/056548 US2011056548W WO2012054391A2 WO 2012054391 A2 WO2012054391 A2 WO 2012054391A2 US 2011056548 W US2011056548 W US 2011056548W WO 2012054391 A2 WO2012054391 A2 WO 2012054391A2
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composition
composition according
ppm
biocide
acid
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PCT/US2011/056548
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WO2012054391A3 (fr
Inventor
Dale A. Grinstead
Kathleen J. Bixler
Ying Zhou
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Diversey, Inc.
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Publication of WO2012054391A2 publication Critical patent/WO2012054391A2/fr
Publication of WO2012054391A3 publication Critical patent/WO2012054391A3/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants

Definitions

  • the disclosure relates to compositions and methods for reducing microbial activity on a surface.
  • compositions that have a low use concentration of active ingredient and meet performance standards for food contact surfaces sanitizers are desired.
  • the disclosure provides a composition for reducing microbial levels on a surface, the composition comprising a biocide and a surfactant comprising a zwitterion.
  • the composition comprises a ratio of biocide to zwitterion of about 4:1 to about 8:1.
  • the surfactant comprises a betaine.
  • the disclosure provides a biocidal composition
  • a biocidal composition comprising a biocide and a surfactant comprising a zwitterion, wherein the composition is substantially free of an alky! amine.
  • the disclosure provides an antimicrobial composition comprising about 30 to about 2000 ppm peracetic acid, and a surfactant comprising a zwitterion, wherein the composition is substantially free of an alkyl amine, and wherein the peracetic acid and the zwitterion are present in the composition at a ratio of about 4:1 to about 8:1.
  • compositions having synergistic biocidal activity wherein the composition comprises cocobetaine and a biocide in a ratio of about 4:1 to about 8:1, and in an amount effective to reduce the amount of biocide required for biocidal activity by at least about 20% to about 90% relative to the same composition without the cocobetaine.
  • Embodiments also provide for a method of making a synergistic biocidal composition.
  • the disclosure provides a method of reducing microbial levels or microbial activity on a surface or airborne microbial levels or microbial activity, the method comprising contacting the surface the air with an effective amount of a composition described herein.
  • Figure 1 is a graph of the number of carriers with no growth after 10 min contact time for various surfactant blends.
  • Figure 2 is a graph of the number of carriers with no growth after 1 min contact time for various surfactant blends with 50 ppm peracetic acid (PAA).
  • PAA ppm peracetic acid
  • Figure 3 is a graph of the mean log reduction in Salmonella choleraesuis with 25 ppm and 50 ppm PAA.
  • Figure 4 is a graph of the mean log reduction in Pseudomonas aeruginosa with 25 ppm and 50 ppm PAA.
  • Figure 5 is a graph of the mean log reduction in Staphylococcus aureus with 25 ppm and 50 ppm PAA.
  • Figure 6 is a graph of the mean log reduction in Staphylococcus aureus or Pseudomonas aeruginosa with 0 and 0.3 mUL cocobetaine.
  • Figure 7 is a quadratic fit to the log reduction in Staphylococcus aureus (7A) or Pseudomonas aeruginosa (7B) with various molar ratios of betaine to PAA.
  • biocidal e.g., antimicrobial compositions comprising a biocide in combination with a surfactant that can potentiate the efficacy of the biocide.
  • the disclosure provides a composition comprising a surfactant and a biocide.
  • a composition that is effective for reducing microbial levels or microbial activity on a surface and/or airborne microbial levels or microbial activity.
  • the composition comprises a biocide and a surfactant in amounts that provide for a synergistic biocidal (e.g., antimicrobial) effect (i.e., provide effective biocidal activity at lower amounts/concentration of the biocide, relative to the biocide without the surfactant).
  • the compositions meet efficacy standards for a food contact surface sanitizer and/or disinfectant.
  • the composition can comprise, consist essentially of, or consist of a biocide and a surfactant, as well as any other recited component.
  • the surfactant can be classified generally as an amphoteric surfactant (e.g., a surfactant comprising a zwitterion).
  • the zwitterion comprises one or more compounds generally known as, or that can be classified as, a betaine such as, for example, glycine betaine, proline betaine, cocobetaine, and cocoamidopropyl betaine.
  • the surfactant comprises cocobetaine.
  • the surfactant can comprise a blend of more than one surfactant.
  • the surfactant may be present in the composition in an amount of at least about 5 ppm, at least about 10 ppm, at least about 20 ppm, at least about 40 ppm, at least about 60 ppm, at least about 80 ppm, at least about 100 ppm, at least about 200 ppm, at least about 500 ppm, at least about 800 ppm, at least about 1000 ppm, at least about 1500 ppm, at least about 2000 ppm, at least about 2500 ppm, at least about 3000 ppm, at least about 3500 ppm, at least about 4000 ppm, or at least about 4500 ppm.
  • the surfactant may be present in the composition in an amount of less than about 5000 ppm, less than about 4500 ppm, less than about 4000 ppm, less than about 3500 ppm, less than about 3000 ppm, less than about 2500 ppm, less than about 2000 ppm, less than about 1500 ppm, less than about 1000 ppm, less than about 800 ppm, less than about 500 ppm, less than about 200 ppm, less than about 100 ppm, or less than about 80 ppm.
  • the composition may comprise a range of surfactant, suitably from about 5 ppm to about 5000 ppm.
  • the composition comprises surfactant having a range of about 20 or 25 ppm to about 200 ppm (e.g., 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, or 200 ppm) or within a range of about 50 ppm to about 100 ppm surfactant.
  • the surfactant is present in the composition in amount effective to provide an additive biocidal effect (i.e., the surfactant can contribute to the biocidal activity of the composition).
  • the surfactant is present in the composition in amount effective to provide a synergistic biocidal effect (i.e., the biocidal activity of the composition is greater than expected based on the amount of biocide and surfactant).
  • the biocide in some embodiments, can be selected from any variety compound classes that exhibit a general reduction in the activity of a target organism such as, for example, a microbe, plant, rodent, or insect.
  • a biocide include compounds typically classified as a pesticide and/or an antimicrobial.
  • the biocide can comprise one or more pesticides such as, for example, a fungicide, an herbicide, an insecticide, an algicide, a molluscicide, a miticide, and/or a rodenticide.
  • a biocide can comprise an antimicrobial agent such as, for example, a germicide, an antibiotic, an antibacterial, an antiviral, an antifungal, an antiprotozoal, and/or an antiparasitic.
  • biocides include, but are not limited to, compounds comprising one or more halogens (e.g., chlorine, chlorite, chlorine dioxide, iodine, and bromine); organic acids (e.g., lactic, acetic, and propionic acid); peroxides (e.g., hydrogen peroxide) and peracids comprising at least 2 carbon atoms (e.g., peracetic acid); surfactants (e.g., cationic, anionic, non-ionic, and zwitterionic surfactants); metals (e.g., silver, copper, and zinc); essential oils; and protein/amino acid based agents (e.g., bacteriocins, lysozyme, and bacteriophage).
  • halogens e.g., chlorine, chlorite, chlorine dioxide, iodine, and bromine
  • organic acids e.g., lactic, acetic, and propionic acid
  • peroxides e.
  • the biocide can be selected from the non-limiting group of, quaternary ammonium compounds (e.g., ammonium chloride), peroxides (e.g., hydrogen peroxide), and peracids (e.g., peracetic acid (PAA)).
  • the biocide comprises peracetic acid.
  • the biocide may be present in the composition in an amount that provides the desired biocidal effect.
  • the composition comprises an amount of biocide of at least about 1 ppm, at least about 10 ppm, at least about 20 ppm, at least about 30 ppm, at least about 35 ppm, at least about 40 ppm, at least about 50 ppm, at least about 100 ppm, at least about 50 ppm, at least about 200 ppm, at least about 250 ppm, at least about 300 ppm, at least about 350 ppm, at least about 400 ppm, at least about 450 ppm, at least about 500 ppm, at least about 1000 ppm, at least about 2000 ppm, at least about 5000 ppm, at least about 8000 ppm, or at least about 10,000 ppm.
  • the biocide may be present in the composition in an amount of less than about 10,000 ppm, less than about 8000 ppm, less than about 5000 ppm, less than about 2000 ppm, less than about 1500 ppm, less than about 1000 ppm, less than about 800 ppm, or less than about 500 ppm.
  • compositions may comprise a range of biocide that is typically from about 1 ppm to about 10,000 ppm, about 30 ppm to about 2000 ppm, about 1000 ppm to about 2000 ppm. Suitable amounts of biocide can be determined by one of skill in the art using any appropriate method, including those described herein.
  • the biocide comprises peracetic acid in the above ranges, including a range of about 35 ppm to about 45 ppm.
  • the biocide and the surfactant may be present in the composition at various ratios.
  • the biocide and the surfactant may be present in the composition at a ratio of at least about 2.5:1 , at least about 2.8:1 , at least about 3:1 , at least about 3.2:1, at least about 3.5:1 , or at least about 4:1.
  • the biocide and the surfactant may be present in the composition at a ratio of less than about 8:1, less than about 7.8:1, less than about 7.5:1, less than about 7.2:1 , less than about 7:1 , less than about 6.8:1 , less than about 6.5:1 , less than about 6.2:1 , less than about 6:1 , less than about 5.8:1 , less than about 5.5:1, less than about 5.2:1 , or less than about 5:1.
  • the biocide and the surfactant may be present in the composition at a ratio of about 2.5:1 to about 8:1 , or about 3:1 to about 7:1.
  • a synergistic composition exhibits a biocidal effect that is greater than an expected additive effect provided by the surfactant or another active agent or composition component.
  • the composition can have a broad pH range of about 2 to about 12.
  • the composition has a phi that is neutral to slightly acidic.
  • the composition has a broad pH range of about 2 to about 12.
  • the composition has a phi that is neutral to slightly acidic.
  • composition has a pH of about 4 to about 6 (e.g., about 4.0, about 4.2, about 4.5, about 4.8, about 5.0, about 5.2, about 5.5, about 5.8, or about 6.0), inclusive of any intervening pH value.
  • the composition may comprise an acid or buffer to maintain pH.
  • Acids may include inorganic acids and organic acids such as, for example, citric acid, nitric acid, phosphoric acid, octanoic acid, methyl sulfonic acid, and sulfuric acid.
  • the composition comprises, consists essentially of, or consists of peracetic acid and cocobetaine.
  • compositions described herein can further comprise one or more chelating agents (chelants).
  • Chelating agents may include, but are not limited to, L-glutamate-N-N-diacetic acid (GLDA), ethylenediaminetetraacetic acid (EDTA), methyl glycine diacetic acid ( GDA), hydroxy iminodiacetic acid (HIDA), nitrilotriacetic acid (NTA), and citrate.
  • the compositions may comprise chelant in an amount of at least about 1%, at least about 2%, at least about 3%, at least about 4%, or at least about 5% by weight.
  • compositions may comprise chelant in an amount of less than about 15%, les than about 14%, less than about 13%, or less than about 12% by weight. In certain embodiments, compositions may comprise about 0-15% chelating agent, or about 5% to about 12% chelating agent by weight. In certain embodiments, the chelating agent comprises GLDA.
  • the compositions may have reduced odor compared to other compositions.
  • the compositions may have reduced corrosion compared to other compositions.
  • the compositions may be substantially free of alkyl amine. As used herein, “substantially free” means having less than about 3%, less than about 1%, less than about 0.5%, or less than about 0.2%, and more particularly about 0.2% to 0% of component.
  • compositions may be formulated for a particular route of delivery or application.
  • the compositions may be in the form of a spray, aerosol, gel, film, wipe, solid (e.g., particulate or powder), suspension, or solution, and applied using any suitable route, apparatus, and/or mechanism for the delivery or application of the compositions and formulations.
  • Such formulation and application strategies can be determined based on the intended or desired characteristics or use of the composition/formulation and are within the ability of one or ordinary skill in the art.
  • biocidal activity can reduce, slow, or stop growth and/or proliferation, reduce, slow, or stop the rate of growth and/or proliferation, or stun, inactivate, or kill an organism such as, for example, a microbe.
  • the term can also relate to reducing, inhibiting, or preventing cellular differentiation in an organism.
  • biocidal activity includes, for example, pesticidal, antiparasitic, larvicidal, antimicrobial, antibacterial, antiviral, antifungal, antiseptic, anti-mold, antibiotic, disinfectant, or sanitization activity.
  • the biocide can reduce the proliferation of an organism. In some embodiments, the biocide can slow proliferation of an organism. In some embodiments, the biocide can stop the proliferation of an organism (e.g., act as a bacteriostatic or fungistatic agent). In some embodiments, the biocide can kill an organism. In embodiments, the disclosure provides a composition that is effective to reduce microbial levels on a surface. For example, microbial levels can be reduced by at least about 2 log units to as much as 10 log units or more (2, 3, 4, 5, 6, 7, 8, 9, 10 log units or more).
  • compositions have biocidal activity against pathogenic organisms including, but not limited to, bacteria, viruses, protists, helminths, mold, yeast, and spore- forming organisms.
  • pathogenic organisms including, but not limited to, bacteria, viruses, protists, helminths, mold, yeast, and spore- forming organisms.
  • bacteria include, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella choleraesuis, and Escherichia coli.
  • the biocidal activity of a composition or component may be determined using various methods known by those of skill in the art. Suitable methods are described below in the Examples.
  • the AOAC Germicidal and Detergent Sanitizer test may be used (AOAC Official Method 960.09. Germicidal and Detergent Sanitizing Action of Disinfectants, AOAC Official Methods of Analysis, 995, Chapter 6, page 9, incorporated herein by reference in its entirety).
  • Use Dilution tests may be used (AOAC Official Method 955.14. Testing Disinfectants against Salmonella choleraesuis, Use- Dilution Method, 1998, Chapter 6, page 4, incorporated herein by reference in its entirety).
  • the EN 1276 test may be used (British Standard BS EN 1276:1977. Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas - test method and requirements, phase 2, stepl , incorporated herein by reference in its entirety).
  • the disclosure provides a method of reducing microbial levels and/or microbial activity on a surface or reducing airborne microbial levels and/or microbial activity, wherein the method comprises contacting the surface or the air with an effective amount of the composition described herein.
  • the disclosure provides a method of disinfecting a surface or air wherein the method comprises contacting the surface or the air with an effective amount of the composition described herein.
  • the methods can comprise contacting a surface such as, for example, the surface of a floor, counter, wall, or other hard surface.
  • the surface can be a porous or non-porous organic or inorganic material.
  • the surface can comprise materials such as, for example, plastics (e.g., polymers comprising
  • the surface can comprise a food contact surface.
  • at least a portion of the surface can comprise a non-food contact surfaces.
  • the surface can comprise organic material such as, for example, water, food or foodstuffs (e.g., meat, dairy, fish, bread, cereal, fruit, vegetable, grain, flour, sugar, salt, spices, herbs, processed foods, etc.), plants, and animals, including skin, such as human and/or animal skin.
  • compositions may be applied to a surface and exposed for a contact time that is sufficient to provide the desired level of biocidal activity.
  • a surface may be contacted for a contact time of at least about 20 seconds, at least about 30 seconds, at least about 45 seconds, at least about 1 min, at least about 2 min, at least about 4 min, at least about 6 min, at least about 8 min, at least about 10 min, at least about 15 min, or at least about 20 min.
  • Surfaces may be contacted for a contact time of less than about 20 min, less than about 15 min, less than about 10 min, less than about 8 min, less than about 6 min, less than about 4 min, less than about 2 min, less than about 1 min, less than about 45 seconds, or less than about 30 seconds.
  • Surfaces may be contacted for a contact time of about 30 seconds. Surfaces may be contacted for a contact time of about 1 min.
  • the disclosure provides a composition comprising synergistic amounts of a biocide and a surfactant.
  • the biocide comprises peracetic acid.
  • the surfactant comprises cocobetaine.
  • Other embodiments relate to a method for preparing a synergistic composition.
  • the biocide comprises peracetic acid and the surfactant comprises betaine. In such
  • the peracetic acid and the cocobetaine may be present in a ratio of about 4:1 to about 8:1 (peracetic acid:cocobetaine).
  • the composition has a pH in a range of slightly acidic to about neurtral (e.g., about 4 to about 6).
  • the addition of cocobetaine to the composition comprising peracetic can provide for a lesser amount (from about 20% to about 50% less) of peracetic acid in order to reduce microbial levels on a surface by at least 3 log units relative to the same peracetic composition in the absence of cocobetaine.
  • UD Use-Dilution
  • AOAC Official Method 955.14 was used to test disinfectants against microorganisms, such as Salmonella choleraesuis, Staphylococcus aureus, and Pseudomonas aeruginosa.
  • Ring carriers were soaked overnight in 1 N NaOH, rinsed with tap water until rinse water was neutral to phenolphthalein, and then rinsed twice with distilled water. Clean ring carriers were placed in multiples of 10 in cotton-plugged Erlenmeyer flasks or 25 x 150 mm cotton plugged Pyrex test tubes, covered with asparagine solution, sterilized 20 min at 121°C, cooled, and held at room temperature. For the asparagine solution, a 0.1% solution in water was made in an Erlenmeyer flask of convenient size, plugged with cotton, and sterilized for 20 min at 121 °C. Nutrient broth test culture was vortex-mixed for 3-4 sec and stood for 10 min at room temperature before continuing.
  • a dilution of germicide to be tested was prepared by dilution in sterile water.
  • the sterile water had been prepared by sterilizing for 20 min at 121 °C in flasks.
  • Dilutions of sample were made using ⁇ 1.0 ml_ of sample.
  • Volume/volume dilutions were used for liquid products and weight/volume dilutions were used for solids. Results were rounded to two decimal places toward a stronger product.
  • a solution was prepared ⁇ 3 h prior to use. Tubes were placed in a 20°C water bath for at least 10 min.
  • the dilution to be tested was determined by multiplying the phenol coefficient number found and/or claimed by 20 to determine the number of parts of water in which one part germicide was to be incorporated. This determination was not required when the disinfectant under test yielded a phenol coefficient that could not be converted validly to presumptive use- dilution, or when an analyst determined that a use-dilution range could be found without resort to the phenol coefficient test.
  • test culture suspension was added to 1-90 dilution of phenol control. After a 30 sec interval, 0.5 mL was added to 1-100 dilution of control, using sterile cotton- plugged pipettes. After adding culture, the tubes were agitated gently but thoroughly to distribute bacteria evenly, and replaced in the water bath. 5 min after seeding the first medication tube, one loopful of mixture of culture and diluted phenol was transferred from the medication tube to the corresponding subculture tube. After 30 sec, a loopful was transferred from the second medication tube. 5 min after making the first set of transfers, the second set of transfers for a 10 min period was begun. It was repeated for a 15 min period.
  • each ring was optionally transferred to new tube of sterile medium and re-incubated for an additional 48 h at 37°C.
  • solution under test was such that material adsorbed on ring carriers and transferred into subculture medium made it unsuitable for growth of the test organism, as may be case with concentrated acids and alkalies, products carrying antibiotics, and wax emulsions
  • each ring was transferred to a new tube of sterile medium 30 min after the initial transfer and both primary and secondary subculture tubes were incubated for 48 h at 37°C.
  • the maximum dilution of germicide which killed both this test organism and S. choleraesuis on 10 carriers in a 10 min interval represented the maximum presumed safe use-dilution for disinfecting in hospitals, clinics, and other places where pyogenic bacteria have special significance, it is noted that while killing in 10 of 10 replicates specified provided a reasonably reliable index in most cases, killing in 50 of 60 replicates provided a confidence level of 95%.
  • Vortex-mixing was done as described above prior to use of culture.
  • the pellicle may be carefully suctioned off, and culture can be poured into a clean, sterile tube before vortex-mixing. Any disruption of pellicle resulting in dropping, breaking up, or stringing of pellicle in culture before or during its removal rendered that culture unusable in use-dilution test. Any pellicle fragments remaining may have resulted in uneven clumping and layering of the organism on the cylinder, allowing unfair exposure to disinfectant and causing false positive results.
  • Example 1 Preliminary disinfectant testing.
  • UD tests as described in Reference Example 1 , were conducted with various combinations of surfactants and biocides. Biocides and surfactants were mixed in a beaker. The biocides and the surfactants tested are shown in Table 1. Table 1. Samples examined in UD screening tests.
  • a surfactant blend and a biocide were mixed together.
  • the surfactant blend listed in Table 1 was diluted 1 :256 in 400 ppm hard water and biocide to yield the final biocide concentrations listed in Table 1.
  • In this series of UD tests only 10 carriers were used. Although the test normally calls for 60 carriers to be used in each test, ten carriers are frequently used for screening of multiple samples. This testing was conducted against Pseudomonas aeruginosa ATCC 15442. Samples were tested at 1 min or 10 min contact times. Results are summarized in Figure 1 (10 min contact time). The tests were repeated for solutions comprising 40 ppm PAA and various surfactant blends with a 1 min contact time, and results are presented in Figure 2.
  • Example 2 Additional disinfectant testing.
  • Example 1 the solutions with the greatest biocidal activity contained cocobetaine, PAA, and GLDA. Additional tests were conducted to further investigate the potential of cocobetaine surfactant (and GLDA) to synergize the biocida! efficacy of PAA against P. aeruginosa and Staphylococcus aureus in UD screening tests each employing 10 carriers. A full factorial design was used and the data were analyzed with the JMP statistical analysis software. Table 2 describes the full factorial design used in these experiments and provides the results of that testing. The exposure time used was reduced to 5 min, to ensure that the PAA would not have sufficient activity on its own to kill the test organisms. All samples were prepared in 400 ppm hard water. These data were fit to a least squares model and results of that analysis are presented in Table 3 and Table 4.
  • Detergent Sanitizer test That test is the one that must be used to demonstrate the
  • Test organisms were P. aeruginosa, S. aureus, and Salmonella choleraesuis. Table 5 describes the samples tested and the reduction in log
  • PAA level(25,50) 1.4475926 0.161533 8.96 ⁇ 0001*
  • PAA affected the biocidal activity of the solutions. Specifically, more PAA in the sample resulted in a greater microbial reduction. The presence of a biocide significantly further enhanced the microbial reduction. Unexpectedly, cocobetaine had a significant effect for P. aeruginosa for which it reduced the biocidal performance of samples in which it was used. The cocobetaine level was also significant for the S. aureus for which it increased the biocidal performance. The GLDA was also significant for both S. choleraesuis and P.
  • Example 4 European disinfection tests.
  • cocobetaine 3300 ppm cocobetaine; 0.8 g/L nonionic blend; 20 ppm PAA; 60 ppm
  • PAA 60 ppm octanoic acid; in 400 ppm hard water
  • Samples 7 through 9 contained the coco amidopropyl betaine but did not show significant biocidal activity, which suggested that not all betaines would function in this system. Further, pH may have a significant effect on this system.
  • Citric 40 0 4 2 4.17 5.18/p.a
  • Citric 40 0 4 0.91 4.09
  • Citric 40 0 4 1.01 2.98
  • Cocobetaine level (mlJL)(0,0.3) 1 1 43.707735 182.3025 ⁇ .0001*
  • the formulation with optima biocidal activity comprised approximately 40 ppm PAA and cocobetaine at approximately a 6:1 molar ratio, with a pH of approximately 5.0.
  • compositions and methods disclosed herein are effective for reducing microbial levels on a surface.

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Abstract

Les compositions pour réduire les niveaux microbiens sur une surface ci-décrites comprennent un biocide et un tensioactif zwitterionique présents dans la composition dans un rapport d'environ 4:1 à environ 8:1. Des procédés et des compositions qui contiennent une quantité réduite de biocide pour réduire les niveaux microbiens effectifs sur une surface sont également décrits.
PCT/US2011/056548 2010-10-18 2011-10-17 Compositions et procédés pour réduire les niveaux microbiens sur une surface WO2012054391A2 (fr)

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EP3223611B1 (fr) 2014-11-28 2020-06-03 Ecolab USA Inc. Compositions desinfectante à deux composants contenant de l'acide peracétique et des agents chélateurs

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US20060233886A1 (en) * 2005-03-31 2006-10-19 Kielbania Andrew Jr Antimicrobial composition and method for making same
US20060285995A1 (en) * 2005-06-15 2006-12-21 3M Innovative Properties Company Compositions and methods of use
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EP3223611B1 (fr) 2014-11-28 2020-06-03 Ecolab USA Inc. Compositions desinfectante à deux composants contenant de l'acide peracétique et des agents chélateurs
US11140896B2 (en) 2014-11-28 2021-10-12 Ecolab Usa Inc. Two components disinfecting composition containing peracetic acid and chelating agent

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