WO2012052727A3 - Cells and devices - Google Patents

Cells and devices Download PDF

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Publication number
WO2012052727A3
WO2012052727A3 PCT/GB2011/001518 GB2011001518W WO2012052727A3 WO 2012052727 A3 WO2012052727 A3 WO 2012052727A3 GB 2011001518 W GB2011001518 W GB 2011001518W WO 2012052727 A3 WO2012052727 A3 WO 2012052727A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
devices
population
transgene
astrocyte
Prior art date
Application number
PCT/GB2011/001518
Other languages
French (fr)
Other versions
WO2012052727A2 (en
Inventor
Patrick Degenaar
Ivo Lieberam
Linda Greensmith
Original Assignee
King's College London
Imperial Innovations Limited
Ucl Business Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by King's College London, Imperial Innovations Limited, Ucl Business Plc filed Critical King's College London
Publication of WO2012052727A2 publication Critical patent/WO2012052727A2/en
Publication of WO2012052727A3 publication Critical patent/WO2012052727A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
    • C12N2502/081—Coculture with; Conditioned medium produced by cells of the nervous system neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
    • C12N2502/086—Coculture with; Conditioned medium produced by cells of the nervous system glial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The invention related to an in vitro population of cells comprising (i) a motor neuron comprising a transgene; said transgene encoding a photosensitive protein capable of triggering or suppressing an action potential upon illumination with light of cognate wavelength; and (ii) an astrocyte. The invention also provides an implantable device comprising a population of cells as described above, wherein said device is structured to retain the cell bodies within the device.
PCT/GB2011/001518 2010-10-21 2011-10-21 Cells and devices WO2012052727A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1017820.0A GB201017820D0 (en) 2010-10-21 2010-10-21 Cells and devices
GB1017820.0 2010-10-21

Publications (2)

Publication Number Publication Date
WO2012052727A2 WO2012052727A2 (en) 2012-04-26
WO2012052727A3 true WO2012052727A3 (en) 2015-07-16

Family

ID=43334199

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2011/001518 WO2012052727A2 (en) 2010-10-21 2011-10-21 Cells and devices

Country Status (2)

Country Link
GB (1) GB201017820D0 (en)
WO (1) WO2012052727A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9782091B2 (en) 2011-07-25 2017-10-10 Neuronexus Technologies, Inc. Opto-electrical device for artifact reduction
WO2013016392A1 (en) 2011-07-25 2013-01-31 Neuronexus Technologies, Inc. Neuromodulation transfection system with passive fluid delivery
WO2013016391A1 (en) 2011-07-25 2013-01-31 Neuronexus Technologies, Inc. Neuromodulation transfection system with means for active fluid delivery and a method for its use
CN104846014B (en) * 2015-02-28 2017-12-26 中国科学院深圳先进技术研究院 The composition of special heat star spongiocyte and its application in schizophrenia difference behavior is improved
EP3586915A1 (en) * 2018-06-25 2020-01-01 BIOTRONIK SE & Co. KG Device for activating of cell structures by means of electromagnetic energy

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040259071A1 (en) * 2003-04-23 2004-12-23 Rush University Medical Center Erythropoietin administration to improve graft survival
WO2008014382A2 (en) * 2006-07-26 2008-01-31 Case Western Reserve University System and method for controlling g-protein coupled receptor pathways

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7390659B2 (en) 2002-07-16 2008-06-24 The Trustees Of Columbia University In The City Of New York Methods for inducing differentiation of embryonic stem cells and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040259071A1 (en) * 2003-04-23 2004-12-23 Rush University Medical Center Erythropoietin administration to improve graft survival
WO2008014382A2 (en) * 2006-07-26 2008-01-31 Case Western Reserve University System and method for controlling g-protein coupled receptor pathways

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HAN XUE ET AL: "Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain", NEURON, vol. 62, no. 2, 30 April 2009 (2009-04-30), pages 191 - 198, XP028874948, ISSN: 0896-6273, DOI: 10.1016/J.NEURON.2009.03.011 *
KONSTANTIN NIKOLIC ET AL: "Modeling and Engineering aspects of ChannelRhodopsin2 System for Neural Photostimulation", CONFERENCE PROCEEDINGS. ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (IEEE CAT. NO. 06CH37748); 30 AUG.-3 SEPT. 2006; NEW YORK, NY, USA, IEEE, PISCATAWAY, NJ, USA, 1 August 2006 (2006-08-01), pages 1626 - 1629, XP031186691, ISBN: 978-1-4244-0032-4 *
MELYAN Z ET AL: "Addition of human melanopsin renders mammalian cells photoresponsive", NATURE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 433, no. 7027, 17 February 2005 (2005-02-17), pages 741 - 745, XP002382403, ISSN: 0028-0836, DOI: 10.1038/NATURE03344 *
PATRICK DEGENAAR ET AL: "Optobionic vision-a new genetically enhanced light on retinal prosthesis; Optobionic vision", JOURNAL OF NEURAL ENGINEERING, INSTITUTE OF PHYSICS PUBLISHING, BRISTOL, GB, vol. 6, no. 3, 1 June 2009 (2009-06-01), pages 35007, XP020162278, ISSN: 1741-2552 *

Also Published As

Publication number Publication date
GB201017820D0 (en) 2010-12-01
WO2012052727A2 (en) 2012-04-26

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