WO2012050857A1 - Procédé de lutte biologique contre les fourmis coupeuses de feuilles - Google Patents

Procédé de lutte biologique contre les fourmis coupeuses de feuilles Download PDF

Info

Publication number
WO2012050857A1
WO2012050857A1 PCT/US2011/053582 US2011053582W WO2012050857A1 WO 2012050857 A1 WO2012050857 A1 WO 2012050857A1 US 2011053582 W US2011053582 W US 2011053582W WO 2012050857 A1 WO2012050857 A1 WO 2012050857A1
Authority
WO
WIPO (PCT)
Prior art keywords
spores
dry
carrier
nest
composition
Prior art date
Application number
PCT/US2011/053582
Other languages
English (en)
Inventor
Hubert Herz
William Thomas Wcislo
Cameron Robert Currie
Original Assignee
Smithsonian Institution
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smithsonian Institution filed Critical Smithsonian Institution
Publication of WO2012050857A1 publication Critical patent/WO2012050857A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/34—Aspergillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma

Definitions

  • the present invention relates to methods and
  • compositions for controlling leaf-cutting ants using biological controls More specifically, the invention relates to such methods and compositions in which dry vegetative spores of a mycopathogen, such as Escovopsis spp. , are combined with a dry carrier and applied to a nest of leaf-cutting ants.
  • the spores germinate and give rise to fungi that parasitize and destroy the ant-cultivated fungus that is the ant colony's food source, thereby promoting destruction of the ant colony.
  • Leaf-cutting ants (genera Atta and Acromyrmex, family: Formicidae) live in an obligate mutualism with a fungus (Leucocoprinus gongylophorus, Lepiotaceae, Basidiomycota) for which they cut and collect live vegetative material.
  • the ants bring fresh leaves, flowers and fruits to their colony nest, process this material by shredding it into small particles and incorporate it into the fungus culture.
  • the fungus grows on this substrate and then is consumed by the worker ants, the brood and the queen as their exclusive food source.
  • Leaf-cutting ants are extremely polyphagous, using up to 50 - 80% of the available species in their territory (Cherrett, J.M. Leaf-cutting ants. In: Lieth, H., Werger, M.J. A. (eds.): Tropical Rain Forest Ecosystems.
  • the colonies can become very large, especially colonies of the genus Atta, in which millions of ants and the nest can occupy large areas of 100 m 2 or more. Mature colonies can consume up to about 900 kg of biomass in a year (Herz, H., Beyschlag, W., Holldobler, B. "Herbivory rate of leaf-cutting ants in a tropical moist forest in Panama at the population and ecosystem scales.” 2007. Biotropica 39: 482-488) .
  • Leaf-cutting ants are very destructive pests in agriculture, horticulture, silviculture and range
  • Biological control methods have been tried, but they have been of limited success.
  • One existing biological control agent uses bait loaded with the entomopathogens Beauveria bassiana and Metarhizium anisopliae and the mycopathogen Trichoderma sp. (Productos Biologicos Perkins Ltda., Palmira-Valle, Colombia). These pathogens, however, are not specialized (i.e., their widespread use may have unintended consequences of killing beneficial soil-dwelling insects or fungi) , and conventional
  • the present invention is directed to a method for controlling a population of leaf-cutting ants which comprises introducing into a leaf-cutting ant nest a composition comprising fungal spores of a mycopathogen which infects anti-cultivated mutualistic fungus mixed with an inert, dry carrier material in an amount sufficient to infect mutualistic fungus present in the nest as the ants' food source and damage or destroy the ants' food source, thereby controlling the ant population.
  • the mycopathogen is a species of Escovopsis .
  • Administration of the mycopathogen infects the mutualistic fungus and colony energy resources are diverted in an effort to control it. Desirably, as a result of the administration of the mycopathogen, there is a reduction in the population of leaf-cutting ants; most desirably, the ant population is eradicated.
  • the invention further is directed to a composition comprising fungal spores of a mycopathogen mixed with an inert, dry carrier material.
  • the invention is directed to a method of damaging or destroying the mutualistic fungus cultivated by leaf-cutting ants which comprises contacting the fungus with fungal spores of a mycopathogen mixed with an inert, dry carrier material in an amount sufficient to parasitize and damage the fungus.
  • a method of damaging or destroying the mutualistic fungus cultivated by leaf-cutting ants which comprises contacting the fungus with fungal spores of a mycopathogen mixed with an inert, dry carrier material in an amount sufficient to parasitize and damage the fungus.
  • Figure 1 is a time course of intensity of infection (% of 25 collected refuse particles containing Escovopsis) after infection (arrow on day 0) of 21 mature, natural leaf-cutting ant colonies (Atta colombica) .
  • Figure 2 is a time course of survival of young, incipient leaf-cutting ant nests after infection with
  • Figure 3 shows the intensity of infection at three Acromyrmex Iron nests after treatment with a mixture of wheat flour as a carrier and dry spores of Escovopsis sp. at a ratio of 1:100 (wt:wt) showing viability of the method.
  • the lower intensity after 5 days reflects the time lag for spore germination and spread of the pathogen within the nest.
  • Figure 4 shows the intensity of infection (% refuse particles with Escovopsis sp.) at three Acromyrmex striatus nests after treatment with a mixture of wheat flour as a carrier and dry spores of Escovopsis sp . at a ratio of 1:100 (wt:wt).
  • the relatively low intensity of infection in comparison with results at Acromyrmex May, Figure 3 above, and Atta colombica , Figure 1 above may be due to an unique nest architecture in that species that was not known by the time tests were made. This architecture makes it difficult to inject the spore mixture with a duster pump deep into the nest.
  • Leaf-cutting ants cultivate a mutualistic fungus ⁇ Leucoagaricus gonglyophorus) in their nests as their food supply. This fungus is subject to infection with
  • the mycopathogen is a
  • specialized mycopathogen refers to a pathogenic microfungus that attacks only the mutualistic fungus cultivated by the leaf- cutting ants.
  • mycopathogen is a parasitic
  • Escovopsis is known exclusively from the fungus gardens of fungus-growing ants (tribe Attini) and is necrotrophic : it secretes compounds that break down and contact host mycelium
  • Escovopsis is a specialized mycopathogen, i.e., it has not been observed attacking other fungi, making it a preferred organism for use in the present invention, as there will be no unintended negative consequences for other organisms upon application.
  • Mycopathogens other than Escovopsis that produce spores in dry form and infect the mutualistic fungus also can be useful in the methods and compositions of this invention. They include species of Trichoderma and
  • Verticillium (Lecanicillium) . These parasites can be used alone or in combination with an Escovopsis microfungus. Suitable species include E. weberi and E. aspergilloides , T. viride, T. harzinium and Verticillium theobromae (Lecanicillium theobromae) . It is important that the spores of the selected microfungus occurs in dry form so that the spores can be mixed with a carrier and dusted into the cavities of the ant nest without losing their
  • Ant entomopathogens that produce spores in dry form also can be useful in the methods and compositions of this invention, most desirably in combination with the dry spores of a mycopathogen, such as those set forth above.
  • Suitable entomopathogens include Metarhizium, Beauveria , Verticillium (Lecanicllium) , Acromonium, Aspergillus, Paecilomyces, Entomophthora , Zoophthora , Pandora and
  • Entomophaga Suitable species include Metarhizium
  • Other biological pesticides that produce small, dry spores also can be used in the methods and compositions of the invention, most desirably in combination with the dry spores of a selected mycopathogen.
  • suitable biological pesticides can include bacteria, microsporidia, viruses, nematodes and protozoans.
  • spores of Escovopsis or other mycopathogen are prepared for infection of nests of leaf-cutting ants. Any of a variety of species of the selected mycopathogen can be used. Isolates of the mycopathogen are grown under sterile conditions in media using standard techniques. A preferred medium is potato dextrose agar (PDA) ; other suitable media include
  • the spores then are mixed with an inert, dry carrier prior to application to the ant nests.
  • a preferred carrier is sawdust. Grain size of the sawdust typically can be between about 0.005 to about 0.24 mm long and about 0.005 to about 0.1 mm wide, with a mixture of sizes typically used .
  • inert, dry carriers that can be used in the present invention include powders, such as mineral powders, including silicic acid, kaolin, active carbon, bentonite, montmorillonite, diatomaceous earth, talc, clay calcium carbonate, alumina, acid clay, talc powder, agalmatolite powder, mica, silica sand and ammonium sulfate, and
  • vegetable powders such as soybean powders, wheat flour, tobacco powder, starch and crystalline cellulose.
  • the carrier is oven dried, air dried, vacuum dried or freeze dried prior to being mixed with the
  • the Escovopsis or other pathogen spores are mixed with the carrier in a ratio of about 1:50 to about 1:2000 wt:wt.
  • the spores are mixed with the carrier in a ratio of about 1:120 to about 1:1500 wt:wt, more preferably in a ratio of about 1:150 to about 1:1000 wt:wt and most preferably in a ratio of about 1:200 to about 1:500 wt:wt.
  • the mixture of spores and carrier are applied to the nest of a leaf-cutting ant colony so as to infect, damage and preferably destroy, the mutualistic fungus growing therein which the ants use as their exclusive food source.
  • the colony begins to weaken and die. Even if not all of the food source is destroyed, the ants have to use significant energy to defend themselves against the pathogen in their nest, resulting in less energy available for population growth, such that colony size can be controlled.
  • the mixture applied to the nest also comprises one or more entomopathogens , those pathogens infect individual ants directly, which then become sick and die.
  • the dry mixture of spores and carrier can be applied by spraying or injecting the mixture into the nest.
  • the dry carrier and spores mixture can be applied using a commercially available dusting device or aerosol applicator.
  • applicators include compression sprayers, such as pump type sprayers with pistons or plungers, motorized dusters, or bulb dusters.
  • An example of a suitable duster is a commercially available plunger-type duster produced by Guarany Industri e Comercio Ltda., Brazil.
  • the reservoir of the duster is filled with the mixture of spores and carrier and attached to the duster.
  • the tube of the duster is inserted into a
  • Ventilation hole or entrance of an ant nest and the plunger is pumped several times to produce an air stream that blows the mixture of spores and carrier into the nest chambers containing the fungus garden.
  • Small leaf-cutting ant nests may have only a single or very limited number of entrances and ventilation holes. Larger nests can have multiple (up to 50 or more) openings. Application into all, or at least a majority, of the entrances and ventilation holes of the nest is desirable to maximize infection success. During application the tube initially is inserted as deeply as possible and then slowly moved to shallower levels to maximize infection of all fungus chambers.
  • Ant colonies in newer, small-scale nests frequently can be reduced or destroyed following a single application of the Escovopsis spores (see, for example, Figure 2) .
  • compositions of the present application are useful for controlling or eradicating populations of the different species.
  • Different species are abundant or problematic in different areas, but particularly widely distributed species include Atta sexdens , A. cephalotes , A. laevigata , Acromyrmex octospinosus, Ac. subterraneus and Ac. landolti . Any and all species which are present in an area and causes damage may be considered a pest and can be subject to control by the method of this invention.
  • mycopathogen-containing powder of the present invention can be administered to the ant nest in combination with dry spores of an entomopathogen, such as Metarhizium anisopliae and Beauveria bassiana, that acts directly on the ants, rather than on their food source.
  • spores of a Metarhizium sp. are mixed with a dry carrier and administered in combination with a dry carrier containing Escovopsis spores.
  • entomopathogen spores can be prepared in accordance with the method described above for preparation of the
  • mycopathogen spores Preferably, the mycopathogen spores and entomopathogen spores are combined with the same carrier and administered as a single composition, but they can also be administered in separate compositions.
  • the ratio of mycopathogen : entomopathogen is within the range of about 90:10 to about 50:50 (wt:wt).
  • Petri dishes (10 cm diameter) prepared with PDA medium were inoculated with 1-3 mm 3 (by volume) of actively growing cultures of one or more species of Escovopsis .
  • four inoculates were placed on each plate, evenly distributed on the medium. Inoculates were taken from the actively growing culture with a sterile needle and were approximately 1 mm 3 in size, containing hyphae. b) Incubating the culture
  • the masses of spores were either collected with forceps (aerial form) or were scraped off the medium with a wide spatula (carpet form) .
  • the spores could not be collected without mycelium, and possibly medium, also being collected, and so the recovered spores were further processed.
  • the collected spores were put in a dish which in turn was placed in a container containing 0.5 kg of a desiccant (silica gel) and allowed to dry more completely for 3 days. This additional drying time both allowed further moisture removal from the collected
  • the dried mycelium with spores was loaded onto a jigger screen as a strainer (hole size approximately 0.75 mm) to separate spores from the mycelium and remnants of medium.
  • the jigger screen was placed on a glass that collected the spores. The space between the screen and the glass was closed with a flexible sealant (Terostat®) to prevent the dust-like spores from escaping. The top of the jigger screen was closed with plain clear plastic wrap.
  • the reservoir of a duster having a volume of about 1000 cm 3 , was filled with the mixture and attached to the duster.
  • the duster used was the plunger-type model sold by Guarany Industria e Comercio Ltda, Rod. Waldomiro Correia de
  • Intensity of infection was determined by occurrence of Escovopsis growth from collected refuse particles from ants leaving the nest, using the procedure of Currie, C.R., "Prevalence and impact of a virulent parasite on a
  • the time lag reflects the time until the spores germinated. In the five control colonies that received only sawdust but no spores, intensity of Escovopsis
  • nest size is measured as the two diameters of the ellipsoid formed by the nest surface
  • colombica (4-5 months old) were identified in a park-like area in Gamboa, Panama, and were randomly assigned to five groups (21 nests per group) .
  • the nests were treated as follows :
  • Group III each nest received 1-1.5 g of a mixture of sawdust and Metarhizium in a ratio of 150:1
  • Group IV each nest received 1-1.5 g of a mixture of sawdust and Escovopsis in a ratio of 150:1
  • Group V each nest received 1-1.5 g of a combination of Metarhizium and Escovopsis spores mixed with sawdust in a ratio of 1:1:150.
  • the sawdust and sawdust mixtures were each injected with a duster as in Example 2 above.
  • the duster was pressed 2-3 times which equals 1-1.5 g of dust.
  • the nests were regularly monitored for activity for just over a month. The results are shown in Figure 2.
  • a combination of eight fungi was tested at three nests of Atta colombica in Gamboa, Panama, that were intermediate between the young and adult nests used in Examples 3 and 4.
  • the nests were two to three years old and well established. From each fungus, 0.25g of spores were mixed in 50 g sawdust, and injected as described in above. Within one month, no mortality or obvious negative effects were observed. However, re-visiting the nests after four months showed that two of the nests were inactive (they either had died or were abandoned) , and only one was still alive and active. The demise is likely to be related to the treatment, since it is highly unusual that established colonies die or disappear.
  • Trichoderma Fungi broad Isolated from leafcutter sp. ant trash (frequently observed in ant trash presumably being deleterious in the nest)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente demande concerne des procédés et des compositions dans lesquels des spores végétatifs secs d'un mycopathogène, comme Escovopsis spp., sont combinés à un support sec et appliqués sur un nid de fourmis coupeuses de feuilles. Les spores germent et produisent des champignons qui parasitent et détruisent le champignon cultivé par les fourmis qui est la source d'alimentation de la colonie de fourmis, en contribuant ainsi à la destruction de la colonie de fourmis. La présente demande concerne également l'utilisation d'une combinaison d'Escovopsis et d'autres entomopathogènes et mycopathogènes.
PCT/US2011/053582 2010-09-29 2011-09-28 Procédé de lutte biologique contre les fourmis coupeuses de feuilles WO2012050857A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38767510P 2010-09-29 2010-09-29
US61/387,675 2010-09-29

Publications (1)

Publication Number Publication Date
WO2012050857A1 true WO2012050857A1 (fr) 2012-04-19

Family

ID=45938629

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/053582 WO2012050857A1 (fr) 2010-09-29 2011-09-28 Procédé de lutte biologique contre les fourmis coupeuses de feuilles

Country Status (1)

Country Link
WO (1) WO2012050857A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160316764A1 (en) * 2015-05-01 2016-11-03 Isca Technologies, Inc. Methods and compositions for controlling leaf-cutting ant populations
WO2017121881A1 (fr) 2016-01-15 2017-07-20 Universite Paris Est Creteil Val De Marne Methodes et compositions pour le controle des fourmis champignonnistes
EP3563671A3 (fr) * 2018-02-15 2020-02-26 Envion OY Dispositif d'étalement et procédé pour contrôler d'agents pathogènes forestiers sur des souches d'arbres
WO2020058196A1 (fr) * 2018-09-19 2020-03-26 Bayer Cropscience Biologics Gmbh Procédé d'augmentation de la stabilité au stockage de spores fongiques

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888989A (en) * 1994-12-21 1999-03-30 Hoechst Aktiengesellschaft Synergistic compositions for controlling insects and acarina
US6344208B1 (en) * 1989-03-20 2002-02-05 Philip Edwin Howse Pheromone baits for social insects
US6442890B1 (en) * 2000-10-31 2002-09-03 Samuel M. Creeger Method of controlling pests and associated apparatus
USRE38958E1 (en) * 1991-04-18 2006-01-31 University Of Florida Research Foundation, Inc. Methods and formulations for control of pests
WO2010044680A1 (fr) * 2008-10-14 2010-04-22 Millennium Microbes Limited Champignons entomopathogènes et leurs utilisations

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6344208B1 (en) * 1989-03-20 2002-02-05 Philip Edwin Howse Pheromone baits for social insects
USRE38958E1 (en) * 1991-04-18 2006-01-31 University Of Florida Research Foundation, Inc. Methods and formulations for control of pests
US5888989A (en) * 1994-12-21 1999-03-30 Hoechst Aktiengesellschaft Synergistic compositions for controlling insects and acarina
US6442890B1 (en) * 2000-10-31 2002-09-03 Samuel M. Creeger Method of controlling pests and associated apparatus
WO2010044680A1 (fr) * 2008-10-14 2010-04-22 Millennium Microbes Limited Champignons entomopathogènes et leurs utilisations

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SILVA ET AL.: "Susceptibility of the ant-cultivated fungus Leucoagaricus gongylophorus (Agaricales: Basidiomycota) towards microfungi.", MYCOPATHOLOGIA, vol. 162, no. ISS. 2, 1 August 2006 (2006-08-01), pages 115 - 119, XP019404226, Retrieved from the Internet <URL:http:llomega.rc.unesp.br/mauricio/curso/bibliografia/6/214!Silva.et.a1.2006.Mycopathologia.pdf> [retrieved on 20110126], doi:10.1007/s11046-006-0037-6 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160316764A1 (en) * 2015-05-01 2016-11-03 Isca Technologies, Inc. Methods and compositions for controlling leaf-cutting ant populations
US10251402B2 (en) * 2015-05-01 2019-04-09 Isca Technologies, Inc. Methods and compositions for controlling leaf-cutting ant populations
WO2017121881A1 (fr) 2016-01-15 2017-07-20 Universite Paris Est Creteil Val De Marne Methodes et compositions pour le controle des fourmis champignonnistes
FR3046716A1 (fr) * 2016-01-15 2017-07-21 Univ Paris Val De Marne Methodes et compositions pour le controle des fourmis champignonnistes
US10888089B2 (en) 2016-01-15 2021-01-12 Universite Paris Est Creteil Val De Marne Methods and compositions for controlling fungus-growing ants
EP3563671A3 (fr) * 2018-02-15 2020-02-26 Envion OY Dispositif d'étalement et procédé pour contrôler d'agents pathogènes forestiers sur des souches d'arbres
EP3785525A1 (fr) * 2018-02-15 2021-03-03 Envion OY Composition pour lutte ou prévention d'agents pathogènes forestiers sur des souches d'arbres
US11432480B2 (en) 2018-02-15 2022-09-06 Envion Oy Spreading device, method and powder-like mixture composition for controlling or preventing forest pathogens on tree stumps
WO2020058196A1 (fr) * 2018-09-19 2020-03-26 Bayer Cropscience Biologics Gmbh Procédé d'augmentation de la stabilité au stockage de spores fongiques
CN112955015A (zh) * 2018-09-19 2021-06-11 拜耳作物科学生物制品有限责任公司 提高真菌孢子的储存稳定性的方法

Similar Documents

Publication Publication Date Title
Burges Formulation of mycoinsecticides
US7951389B2 (en) Mycoattractants and mycopesticides
Bruck Ecology of Metarhizium anisopliae in soilless potting media and the rhizosphere: implications for pest management
Boyetchko et al. Formulations of biopesticides
CN114717164B (zh) 一种贝莱斯芽孢杆菌zlp-101及其应用
US10531666B2 (en) Compositions and methods to reduce the population of wheat-stem sawfly and hessian fly
AU2001296679B2 (en) Mycoattractants and mycopesticides
Nankinga et al. Reduction of banana weevil populations using different formulations of the entomopathogenic fungus Beauveria bassiana
Priwiratama et al. Utilization of fungi for the biological control of insect pests and Ganoderma disease in the Indonesian oil palm industry
AU2001296679A1 (en) Mycoattractants and mycopesticides
WO2007112257A2 (fr) Souche du champignon metarhizium anisopliae pour lutter contre les termites souterrains
Belay et al. Bioassay and pilot mass production of entomopathogenic fungus, Beauveria bassiana for the control of coffee berry borer (Hypothenemus hampei: Scolytidae), Ferrari
WO2012050857A1 (fr) Procédé de lutte biologique contre les fourmis coupeuses de feuilles
US5595746A (en) Insect pest control
KR102126582B1 (ko) 이사리아 자바니카 ft333 균주 또는 이를 함유하는 총채벌레 및 식물탄저병균의 동시방제용 미생물제제
Sewify et al. Using pheromone mass-trapping and the entomopathogenic fungus Beauveria bassiana in IPM programs for controlling the red palm weevil, Rhyncophorus ferrugineus Olivier (Coleoptera: Rhynchophoridae)
Hajek et al. Microbial control of wood-boring insects attacking forest and shade trees
Zulfitri et al. Efficacy of several entomopathogenic microorganism as microbial insecticide against insect pest on chili (Capsicum annum L.)
KR100332484B1 (ko) 곤충병원 사상균 페실로마이세스 푸모소로세우스 에스에프피-198균주, 이를 포함하는 미생물살충제 및 이를 이용하여 솔잎혹파리 및 해충을 방제하는 방법
Khanna et al. Diseases of Agaricus bisporus and their management.
AU666888C (en) Insect pest control
Ahmed Use of entomopathogenic fungi in biological control of cotton mealybug (Phenacoccus solenopsis) and mustard aphid (Lipaphis erysimi)
KR20230058894A (ko) 뿌리응애 방제능을 가지는 메타리지움 펨피기 111 균주 및 이의 용도
GB2380131A (en) Method of controlling rhinoceros beetle, using Metarhizium anisopliae formulated in nutrient-supplemented pellets consisting mycelia
CN113519323A (zh) 应用生物媒介法防治蛀干害虫的方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11833021

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11833021

Country of ref document: EP

Kind code of ref document: A1