WO2012050404A2 - Composition containing ginseng and having nicotine removal effect - Google Patents

Abstract

The present invention relates to a composition containing ginseng and having a nicotine removal effect, and more specifically, to a composition that is capable of quickly discharging the nicotine accumulated inside a smoker's body, minimizing the stress such as anxiety from the lack of nicotine, and easily leading a user to quit smoking, and is formed by mixing: 96-98 wt% of main ingredient powder formed from 20-27 wt% of licorice, 2-6 wt% of crataegus fruit, 1-3 wt% of nothosmyrnium root, 2-5 wt% of acanthopanax, 2-5 wt% of wilfordi root, 3-6 wt% of clove, 8-10 wt% of dried orange peel, 3-6 wt% of radish seed, 5-10 wt% of platycodon, 24-28 wt% of aloe, and 18-20 wt% of lappa fruit; and 2-4 wt% of sub-ingredient powder formed from 74-78 wt% of ginseng, and 22-26 wt% of peppermint. The composition exhibits an excellent nicotine removal effect wherein nicotine is discharged as cotinine, and the effects of significantly relieving the severe stress experienced from nicotine removal, and of preventing respiratory diseases in a smoker.

Description

Nicotine removing effect containing ginseng

The present invention relates to a composition having a nicotine removal effect containing ginseng, more specifically, to quickly release nicotine accumulated in the smoker's body, to minimize stress such as anxiety due to nicotine deficiency, and to quit smoking It relates to a composition having a nicotine removal effect containing ginseng that can be easily induced.

Tobacco is one of the favorite foods, a type of neurostimulant, and nicotine contained in tobacco stimulates the sensory center of the brain.

There are about 40 kinds of toxic carcinogens in the smoke of the tobacco, and representative harmful components include tar, nicotine and carbon monoxide.

In particular, nicotine contained in the smoke of cigarettes is very harmful to health, such as constricting peripheral blood vessels, speeding up the pulse, and increasing cholesterol, causing arteriosclerosis, and because of its addiction, withdrawal phenomenon occurs when trying to quit smoking. Smoking is not easy.

As described above, withdrawal symptoms caused by smoking cessation can be divided into physical symptoms and psychological symptoms. Physical symptoms include sputum, itching, nausea, headache, constipation, diarrhea and increased appetite. Insomnia, loss of concentration, forgetfulness and cognitive impairment occur.

In addition, cigarette tar contains tobacco, as well as various toxic substances and 20 kinds of carcinogens, and contains addictive nicotine.

The nicotine contracts peripheral blood vessels to speed up the pulse to increase blood pressure and increase cholesterol, causing atherosclerosis or problems with the digestive system, and causing many diseases in the respiratory system.

Although many smokers attempt to quit smoking for the above reasons, not only are they easily made, but many of the conventional smoking cessation aids have solved the withdrawal phenomenon by administering a small amount of nicotine by various means when the withdrawal phenomenon occurs.

In order to solve the above problems, the inventors of the Republic of Korea Patent Office Publication No. 553443, No. 626398, 'The composition and method for producing the nicotine removal effect', 'Antioxidant nutrient supplement composition for smokers and smoking history and its preparation Method '.

However, conventional smoking cessation aids or smoking aids for health promotion have the following problems.

(1) There is no countermeasure against illnesses that occur in the respiratory system of smokers due to smoking.

(2) The nicotine elimination effect may cause some problems with mental symptoms such as stress of smokers.

(3) Improved nicotine removal effect is required, and a product that minimizes withdrawal phenomenon due to nicotine removal is required.

In order to solve the above problems, the present invention is 20 to 27% by weight licorice, 2 to 6% by weight hawthorn, 1 to 3% by weight of Bonbon, 2 to 5% by weight, 2 to 5% by weight of sewage, 3 to 6 weight of cloves. %, Dermis 8-10% by weight, 3-6% by weight moth, 5-10% by weight, aloe 24-28% by weight and 18-20% by weight 96-98% by weight of the main material powder, which was extracted with reflux cooling at 80-85 ° C. for 4-6 hours by adding 30% alcohol corresponding to 7 times;

Weighing 74 ~ 78% by weight of ginseng and 22 ~ 26% by weight of raw materials, respectively, and adding 10% spirits, which is 7 times the total weight of raw materials, and refluxed at 80 ~ 85 ℃ for 4-6 hours. Characterized in that the extracted extract was formed by mixing 2 to 4% by weight of the submaterial powder formed into a powder.

According to the composition having a nicotine removal effect containing the ginseng of the present invention the following effects occur.

(1) Protection of the bronchus of smokers is provided by the introduction of mint.

(2) The anti-stress action of ginseng to reduce the severe stress of smokers due to nicotine removal effect.

(3) Nicotine is converted to cotinine and the amount of excreted in the urine increases, which increases the nicotine removal effect in the body.

Figure 1 is a graph of the results of the test for the determination of nicotine content of urine to determine the nicotine removal effect of the nicotine removal composition containing a preferred ginseng of the present invention.

Figure 2 is an experimental result graph for measuring the smoking cessation effect of the composition having a nicotine removal effect containing the preferred ginseng of the invention.

Figure 3 is a graph of the experimental results for measuring the effect of improving respiratory function of the nicotine removal composition containing a preferred ginseng of the invention.

The present invention is 20 to 27% by weight licorice, 2 to 6% by weight of mountain lion, 1 to 3% by weight, 2 to 5% by weight of sesame, 2 to 5% by weight of sewage, 3 to 6% by weight of cloves, dermis 8 ~ 10% by weight, 3 ~ 6% by weight moth, 5 ~ 10% by weight, 7 ~ 20% by weight of aloe and 18 ~ 20% by weight, equal to 7 times the total weight of the material. After extracting while refluxing for 4-6 hours at 80 ~ 85 ℃ to add 30% spirits to the main material powder 96 ~ 98% by weight of the extract formed into a powder;

Weighing 74 ~ 78% by weight of ginseng and 22 ~ 26% by weight of raw materials, respectively, and adding 10% spirits, which is 7 times the total weight of raw materials, and refluxed at 80 ~ 85 ℃ for 4-6 hours. The extracted extract is formed by mixing 2 to 4% by weight of the submaterial powder formed into a powder.

The main ingredient powder (named 'NPLX' powder) is a medicine that has a high frequency of prescription among the herbal medicines listed in the prescription as described in Korean Patent No. 053643, particularly bronchial and lung functions. Twenty-one herbs are selected, and each of them is divided into 10g, extracted with methanol, and nicotine decomposition experiments are selected.

The main material powder is weighed by the unit weight of the main raw material, and then mixed with 30% spirits 7 times the total weight of the main raw material, extracted with reflux cooling for 4 hours to 6 hours at 80 ~ 85 ℃ and dried extracts Form into a powder.

The numerical limit on the temperature at the time of extraction is lower than the extraction rate is lower than 80 ℃, and higher than 85 ℃ was set to the most suitable threshold because only the cost increases without increasing the extraction rate any more.

The submaterial powder is formed by using ginseng and mint as submaterials, respectively, and extracted in a similar manner as the main raw material, but separately separated.

The main material powder and the submaterial powder are separated and extracted separately, and the reason for mixing is that when extracting the main material raw material and submaterial raw materials together and extracting them as in Experiment 1, nicotine decomposing activity is higher than when the main material raw material and submaterial raw material are separated and extracted. Considering the results of experiments falling somewhat and the results of Experiment 2, which measured the effect on the extraction concentration of raw materials, it is appropriate to separate and extract in two stages and mix in powder state.

The mixing ratio of the main material powder and the subsidiary powder powder was 96-98 wt% of the main powder and 2-4 wt% of the subsidiary powder. When the subsidiary powder was 2 wt% or more, as shown in Experiment 3, nicotine decomposition efficiency was high but 4 wt% or more. In this case, considering the results such as the nicotine decomposition efficiency does not increase and the rejection caused by the flavor of the subsidiary material is considered, the threshold value of the subsidiary powder is preferably 2 to 4% by weight.

The ginseng is a perennial herb, the root is used as an important medicinal herb, and main physiologically active ingredients are saponins, phenolic components, polyacetylene components, alkaloid components, and polysaccharides.

The mint is a perennial herb, also called eternal life, with short hairs throughout the plant, long egg-shaped or oval leaves grow, and is known to have various physiological activities such as anti-allergy, antibacterial and anti-allergic.

After mixing the main material powder and the subsidiary powder, sweeteners, acidulants and flavorings may be added to form a beverage composition, or a gum base, sugars and flavorings may be added to form a gum composition.

Hereinafter, a method for producing a composition having a nicotine removal effect including ginseng formed as a preferred embodiment of the present invention and its action will be described in detail with experiments.

The method of preparing the composition of the present invention is first selected by liquorice, hawthorn, Gobon, Ogapi, Sewao, cloves, dermis, nabokjak, Gilkyung, aloe and allies, and then adjusted according to theft and powder in the Pharmacopoeia Prepare the main raw material by weighing it by weight percent.

Thereafter, a solvent in which the alcohol and the washing water are mixed at a weight ratio of 3: 7, respectively, is prepared in an amount equivalent to 7 times the weight of the main raw material, and the main raw material is at a temperature of 80 to 85 ° C. for 4 to 6 hours. Extract reflux.

After reflux extraction, the main raw material is concentrated by evaporation, and then a small amount of excipient is added to adjust the solids content to 20 brix and spray dried to complete the main material powder.

In addition, ginseng and peppermint are carefully selected and sliced, and then adjusted according to theft and powderiness of the Pharmacopoeia, and then weighed by each weight% to prepare raw materials.

Subsequently, the raw material is extracted by reflux in the same manner as the main material raw material extraction method using 10% alcohol as an extraction solvent, concentrated and adjusted to 20brix each solids and then spray-dried to complete the raw material powder.

The main material powder and the subsidiary powder prepared as described above are 96 to 98% by weight and 2 to 4% by weight of the subsidiary powder, respectively, to complete the composition of the present invention.

The finished powder may be formed into a tablet form by mixing an excipient or an additive, or may be formed into a beverage or the like.

Experiment 1) Nicotine Degradation Activity Test (depending on the concentration of the extraction solvent)

Nicotine degradation activity was measured by cell culture. After pre-cultured PCL / PRF5 cells were washed with PBS (phosphate buffered saline), they were incubated in 3 ml of medium containing 10 µl of nicotine stock solution for 24 hours. Add the test material and incubate for an additional hour to measure the converted cotinine content.

At this time, nicotine degrading activity (NDA) is used to coat nicotine during PLC / PRF5 cell culture.

The cotinine content is measured by modifying the method of quantification of cotinine proposed by Barlow et al. (1987) with a wavelength of light of 490 nm.

The experimental materials were mixed at the same time by mixing the main raw material and the subsidiary raw material at the same time in each weight%, and then refluxed using 30% alcohol (control 1), 20% alcohol (control 2) and 10% alcohol (control 3) as extraction solvents, respectively. A control group made of powder was extracted, and the main material and the submaterial were separated and refluxed in the same manner as in the present invention, and each was formed into a powder, and an experimental group including 97% by weight of the main material powder and 3% by weight of the submaterial powder was used.

Table 1

division	Control group 1	Control group 2	Control group 3	Experimental group
NDA	1.23	1.28	1.22	1.41

Table 1 shows the NDA measured values for each of the control groups 1, 2, 3 and the experimental group, the concentration of the extraction material of the raw material is 30% (control group 1), which is the concentration of the extraction solvent of the main material, the present invention When the control group formed with 10% (control group 3) of the raw material extract material or 20% (control group 2) of the average concentration of each extractant and the nicotine degrading activity of the experimental group, the control groups showed low values. You can see that.

From these results, it can be seen that nicotine decomposition activity is more affected by the interaction between the subsidiary material and the main material in the extraction process than the effect of the concentration of the extraction material used to extract the main raw material and the subsidiary material.

Therefore, the main raw material and the subsidiary raw material are separately extracted and mixed in a powder state.

Experiment 2) Extraction Efficiency Evaluation Experiment

In order to determine the concentration of the extraction material suitable for the extraction of the subsidiary material as water (Ex 0), 10% alcohol (Ex 10), 20% alcohol (Ex 20), 30% alcohol (Ex 30), 40 Each of the experimental groups completed by mixing% alcohol (Ex 40) and mixing 3% by weight of the component powder obtained by reflux cooling extraction of the raw material using the respective extraction solvents and 97% by weight of the main material powder of the present invention, respectively, was Ex 0 group and Ex. Nicotine degrading activity (NDA) was measured by forming groups of 10, Ex 20, Ex 30, and Ex 40.

TABLE 2

division	Ex	0 group	Ex	10 group	Ex 20 group	Ex 30 military	Ex 40 group
NDA	1.34	1.41	1.39	1.35	1.22

As shown in Table 2 above, it can be seen that the highest nicotine decomposition activity appeared when the raw material is extracted at a concentration of 10% of the extraction solvent.

Therefore, when combining the results of Experiment 1 and Experiment 2, it is appropriate that the main raw material and the subsidiary raw material are separated and extracted at different concentrations (30% and 10%) of different extraction solvents and mixed in powder form. Able to know.

Experiment 3) Experiment for selecting optimum mixing ratio of subsidiary powder

In order to determine an appropriate mixing ratio of the subsidiary powder to the main powder, the mixing ratio of the subsidiary powder to the main powder is 0 wt% (S0 group), 1 wt% (S1 group), and 2 wt% (S2 group), respectively. , 3% by weight (S3 group), 4% by weight (S4 group), 5% by weight (S5 group), 6% by weight (S6 group), 10% by weight (S10 group) to form a test group Nicotinic activity was measured, respectively.

TABLE 3

division	S0 group	S1 group	S2 group	S3 group	S4 group	S5 group	S6 group	S10 group
NDA	1.32	1.35	1.40	1.41	1.42	1.41	1.41	1.40

As shown in Table 3, it can be seen that the nicotine decomposition activity value is high when the mixing ratio of the subsidiary powder to the main material powder is 2% or more.

However, when the mixing ratio of the subsidiary powder is 4% by weight or more, the rejection due to the flavor of the subsidiary raw materials (ginseng or peppermint-specific flavor) appears and the nicotine decomposition activity is not greatly increased. Therefore, the addition ratio of the subsidiary powder to the main powder It can be confirmed that 2 to 4% by weight is appropriate.

Experiment 4) Anti-stress effect measurement experiment

In order to confirm the anti-stress effect of the composition of the present invention, a rat (SD rat) was used, and the change in the adrenal weight of the rat to which the composition was administered and the change in the blood stress-related hormone corticosterone (corticosterone) in the rat were measured. do.

Corticosterone levels are analyzed by HPLC system using a modification of Harikai et al. (Harikai et al., Biol Pharm Bull 26, pp. 701-708, 2003). At this time, HPLC analysis conditions were used as a mobile phase using μBondapack C18 column (0.39x30cm), acetonitrile: methanol: sulfuric acid solution (32: 4: 64), flow rate is 0.5 / min, injection rate is 40, UV 240nm .

Stress treatment is done by confining a tube of 7.5cm in diameter and 16cm in length for 30 minutes a day, and applying a stress of 3mA for 1 second every 20 seconds for the last 5 minutes.

The experimental group consisted of the non-stressed group, the stressed control group, the experimental group A, and the experimental group B. Each experimental group was assigned 8 males of SD rats of 8 weeks old to free general solid feed (Samyangsa). Prepare by diet.

The experimental group A and the experimental group B are divided according to the amount of the composition of the present invention before the stress treatment, respectively, in the experimental group A, the composition of the present invention dissolved 30mg per Kg weight of each rat in drinking water and administered to the oral cavity, respectively. In experimental group B, the composition of the present invention was dissolved in drinking water by 20 mg per Kg weight of each rat and administered to the oral cavity, respectively. However, the control is stressed but the composition of the present invention is not administered.

After 10 days of treatment by the above method, the blood samples of the heart of the rats were collected for each experimental group, and the adrenal glands were extracted and weighed.

Table 4

	Untreated group	Control	Experimental Group A	Experimental Group B
Weight of Adrenal grand (mg)	48.4 ± 1.2	56.2 ± 0.9	49.3 ± 0.9	48.8 ± 1.4

Table 4 shows the average weight change of the adrenal glands of the rats in each experimental group. The adrenal weight of the untreated group was 48.4 ± 1.2mg, whereas the stressed control group significantly increased to 56.2 ± 0.9mg. It can be seen that.

However, in the experimental group A and the experimental group B subjected to stress treatment, the increase in adrenal weight was significantly decreased (p = 0.05), and thus, the composition of the present invention exhibited a protective mechanism against stress.

Table 5

	Untreated group	Control	Experimental Group A	Experimental Group B
Corticosterone (μg / blood L)	223.8 ± 19.3	642.3 ± 26.2	304.9 ± 15.9	239.4 ± 18.7

Table 5 shows the change in serum corticosterone in rats of each experimental group, the average change in serum corticosterone per 1L blood of the untreated group was 223.8 ± 19.3㎍ compared to the stress treatment One control group was found to increase nearly three-fold to 642.3 ± 26.2 μg.

However, in the experimental group A and the experimental group B treated with stress, the change in the average serum corticosterone per 1 L of blood was significantly decreased (p = 0.05), indicating that the composition of the present invention exerted a defense against stress. .

Table 5 shows the change in serum corticosterone in rats of each experimental group, the average change in serum corticosterone per 1L blood of the untreated group was 223.8 ± 19.3㎍ compared to the stress treatment One control group was found to increase nearly three-fold to 642.3 ± 26.2 μg.

However, in the experimental group A and the experimental group B treated with stress, the change in the average serum corticosterone per 1 L of blood was significantly decreased (p = 0.05), indicating that the composition of the present invention exerted a defense against stress. .

Experiment 5) Experiment of measuring nicotine removal effect and withdrawal phenomenon in human body

In order to find out the effect of nicotine removal and withdrawal phenomenon in the human body of the composition of the present invention, 40 volunteers (subjects) among smoking experience were selected and the dose experiment of the composition of the present invention was conducted.

Twenty of the subjects were allowed to take the smoking cessation composition according to the present invention, and the remaining 20 were to quit smoking while taking a placebo, and the experiment was conducted by the double blind method.

The result of the experiment was to measure the content of each of the nicotine metabolite cotinine released into the urine while smoking for two weeks, respectively, and the results are shown in FIG.

As shown in Figure 1, the experimental group taking the composition of the present invention (solid line display) was noticed that the first day of the test significantly increased the amount of cotinine is nicotine is rapidly decomposed and discharged, after which the cotinine content is rapidly reduced It can be seen that the residual nicotine content in the body is very small.

However, the placebo (placebo) control group (dotted line) can confirm that the concentration of cotinine is gradually released while staying in the body nicotine for a long time while repeating the increase and decrease during the experiment.

Therefore, it can be seen that the composition of the present invention has an effect of rapidly decomposing nicotine contained in a smoker's body to be released into cotinine.

Experiment 6) Experiment of inhibiting withdrawal phenomenon

At the same time as Experiment 5, subjects who participated in Experiment 5 measured the symptoms of concentration, anxiety, appetite, insomnia, and depression on the scale of 5 scales, respectively. The findings were expressed as Total Withdrawal Symptom Scale.

The withdrawal phenomenon index is displayed from a minimum of 5 to a maximum of 25, and high values indicate that the withdrawal phenomenon is more serious, and the results are shown in FIG. 2.

As shown in Figure 2, the experimental group taking the composition of the present invention (solid line) is not significantly changed the degree of withdrawal phenomenon, the control group (placebo) taking placebo is a degree of feeling the withdrawal phenomenon Severe changes were found to be severe.

Therefore, it can be seen that the composition of the present invention has an effect of effectively reducing the withdrawal phenomenon that smokers feel through quitting smoking.

Experiment 7) Survey for measuring respiratory function

In order to examine the effect of improving the respiratory function of the composition of the present invention to select 200 volunteers among the smoking experience randomly 100 people take the composition of the present invention (experimental group), the other 100 people take placebo for 4 weeks In order to measure respiratory function after the (control), a questionnaire technique modified from the European Community Respiratory Health Survey (ECRHS), which is widely used to confirm the prevalence of asthma, was used.

The questionnaire used in this survey was to measure symptoms during the past week and to express the respiratory health index (5) on the scale of breathing during rest, dyspnea after exercise, incidence of coughing and sputum on a five-point scale. Respiratory Health Scale (RHS). RHS is a minimum of 4 to a maximum of 20 points, high values indicate poor respiratory health.

The results of the survey are shown in FIG. 3, as shown in FIG. 3, the experimental group and the control group of volunteers who responded to the first experiment did not show a significant difference in RHS, but after the four weeks of taking the experiment, the experimental group RHS was very low compared to the control group.

Therefore, it can be confirmed that the composition of the present invention is effective in alleviating abnormalities of the respiratory tract.

As described above, the composition of the present invention is not only excellent in nicotine is discharged to the cotinine, that is, excellent nicotine removal effect, can significantly alleviate the severe stress caused by nicotine removal, and also prevents respiratory diseases of smokers, etc. Effect occurs.

In addition, effects such as adopting an optimal composition manufacturing method that can promote the health of the smoker while exhibiting the nicotine removal effect to the maximum.

While the invention has been described with reference to the accompanying drawings, preferred embodiments of the invention, it will be apparent to those skilled in the art that various modifications are possible without departing from the scope of the invention covered by the following claims.

Claims (3)

1. Licorice 20-27% by weight, 2-6% by weight of lioness, 1-3% by weight of Bonbon, 2-5% by weight of Ogapi, 2-5% by weight of sewage, 3-6% by weight of clove, 8-10% by weight of dermis After weighing 3 ~ 6% by weight, 5 ~ 10% by weight, 24 ~ 28% by weight of aloe and 18 ~ 20% by weight of friends, 30% spirits, which is 7 times the total weight of the ingredients, are added. After extracting while refluxing for 4-6 hours at 80 ~ 85 ℃ to extract the extract powder into powder 96 ~ 98% by weight;

   Weighing 74 ~ 78 wt% of ginseng and 22 ~ 26 wt% of peppermint, respectively, and then adding 10% spirits, which is 7 times the total weight of the raw materials, and refluxed at 80 ~ 85 ℃ for 4-6 hours. A composition having a nicotine removal effect, comprising ginseng, characterized in that the extracted extract was formed by mixing 2 to 4% by weight of the sub ingredient powder formed into a powder.
2. A beverage composition formed by adding a sweetener, an acidulant, and a flavoring agent to the composition powder of claim 1.
3. A gum composition formed by adding a gum base, sugars and spices to the composition powder of claim 1.

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