WO2012036414A2 - Cosmetic composition comprising a fermented soybean extract fraction as an active ingredient - Google Patents

Cosmetic composition comprising a fermented soybean extract fraction as an active ingredient Download PDF

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WO2012036414A2
WO2012036414A2 PCT/KR2011/006657 KR2011006657W WO2012036414A2 WO 2012036414 A2 WO2012036414 A2 WO 2012036414A2 KR 2011006657 W KR2011006657 W KR 2011006657W WO 2012036414 A2 WO2012036414 A2 WO 2012036414A2
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fermented soybean
soybean extract
cosmetic composition
extract fraction
active ingredient
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PCT/KR2011/006657
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French (fr)
Korean (ko)
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WO2012036414A3 (en
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김동현
박준성
장희경
김덕희
김한곤
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(주)아모레퍼시픽
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • the present invention relates to a cosmetic composition containing a fermented soybean extract fraction as an active ingredient, and more particularly, by containing a fermented soybean extract fraction of the present invention excellent in cell proliferation effect and cell regeneration effect and anti-aging of the composition It relates to the use as.
  • Cheonggukjang is one of Korea's representative fermented foods made from soybean. It is one of the traditional types of soy sauce, soy sauce, soybean paste, and red pepper paste that have been commonly used to this day. The state has played an important role as a food. Cheonggukjang is made by using Bacillus subtilis called Bacillus subtilis, which is attached to rice straw, and gives its unique taste and smell by enzymes produced by Bacillus subtilis during fermentation. In addition, it produces a large amount of viscous substance, which is a mixture of levan form fructan and polyglutamate derived from protein sugar and raw protein soybeans. It is a high quality fermented soybean with higher protein and fat content.
  • Cheonggukjang fermentation is known to be performed by Bacillus subtilis (Lee et al., J. Micorbiol. Biotechnol, 13, pp. 892-896, 2003), and Bacillus subtilis as described above. Decreases the activity of intestinal rot bacteria and acts to reduce carcinogens such as ammonia, indole and amine, as well as antibacterial action against pathogens.
  • Bacillus subtilis secretes a lot of vitamin B2, improves the liver's detoxification function, shows an excellent detoxification effect on alcohol and tobacco, and stimulates the intestine through the production of organic acids, thereby enhancing digestive activity.
  • the inventors of the present invention while researching to produce a cosmetic that is safe on the skin and has excellent cell proliferation and cell regeneration effects and stability as a product, found that the fermented soybean extract fractions have very excellent cell proliferation and cell regeneration effects.
  • the present invention has been completed.
  • an object of the present invention is to provide a cosmetic composition excellent in cell proliferation and cell regeneration efficacy by containing the fermented soybean extract fraction as an active ingredient and the use as an anti-aging for the composition.
  • the present invention provides a cosmetic composition containing a fermented soybean extract fraction as an active ingredient.
  • the present invention also provides a use as an anti-aging cosmetic composition containing a fermented soybean extract fraction as an active ingredient.
  • the cosmetic composition containing the fermented soybean extract fraction as an active ingredient according to the present invention is excellent in skin cell proliferation and cell regeneration effect to improve the skin regeneration ability for wound recovery, reduced skin elasticity due to skin regeneration ability decline and By preventing the deepening of skin aging, it shows an excellent skin elasticity improving effect and skin aging effect.
  • 1 is a graph measuring the cytotoxicity evaluation of fermented soybean extract fraction, fermented soybean extract.
  • FIG. 2 is a graph showing the cell regeneration effect in human fibroblasts of the fermented soybean extract fractions.
  • Figure 3 is a graph showing the effect of cell regeneration in HaCaT cells of the fermented soybean extract fractions.
  • the present invention provides a cosmetic composition containing a fermented soybean extract fraction as an active ingredient.
  • the fermented soybean extract fraction according to the present invention is filtered by extracting the fermented soybean extract in a concentrated state once more with an organic solvent, for example ethanol After that, the filtrate separated through centrifugation using a membrane filter may be obtained by concentrating under reduced pressure.
  • an organic solvent for example ethanol
  • Fermented soybean extract fraction of the present invention is characterized by having a molecular weight of 3kDa ⁇ 10kDa. It is not preferred at molecular weights below 3 kDa because it shows cytotoxicity.
  • the cosmetic composition of the present invention contains the fermented soybean extract fraction in an amount of 0.0001 to 30% by weight, more preferably 0.1 to 10% by weight based on the total weight of the composition. If the content is less than 0.0001% by weight can not obtain the effect of cell proliferation and cell regeneration by the active ingredient, and more than 30% by weight is not preferable because the increase of the effect is not large compared to the increase in content.
  • Fermented soybean extract fraction according to the present invention is not particularly limited to its use, for example, can be used in pharmaceutical compositions and the like in addition to the cosmetic composition.
  • the formulation of the cosmetic composition of the present invention is not particularly limited and may be appropriately selected as desired.
  • the cosmetic composition of the present invention may be prepared in a formulation such as flexible cosmetics, nourishing cosmetics, massage creams, essences, nutrition creams, packs, skin ointments.
  • HaCaT 10000 cell counts / well were planted in 96-well plates to assess the cytotoxicity of the test substance. After 24 hours of incubation was incubated for 24 hours in a medium containing no FBS. After incubation, the medium was removed, 100 ⁇ l of PBS was added, and UVB 40mJ was irradiated. At this time, the experimental group that does not irradiate UVB was also made. The material was treated by concentration and incubated for 48 hours, and then 100 ⁇ l of MTT solution (0.5mg / mL) was added thereto and incubated in an incubator at 37 ° C. for 3 hours. When the solution was removed, formazan was formed. 100 ⁇ l of DMSO was added and dissolved, and then cytotoxicity was confirmed by measuring absorbance at 570 nm. The results are shown in FIG.
  • the fermented soybean extract fraction according to the present invention was confirmed that the cell proliferation efficiency while being less cytotoxic.
  • the fermented soybean extract has a greater cytotoxicity compared to the fermented soybean extract fraction, and it was found that the cytotoxicity increased in proportion to the concentration.
  • WST-1 was treated and reacted in a 37 ° C. 5% CO 2 incubator for 3 hours. After incubation for 3 hours, the absorbance was measured at 450 nm using a microplate reader to confirm cell regeneration effect. The results are shown in FIGS. 2 and 3.
  • Fermented soybean extract fraction according to the present invention from Figures 2 and 3 was able to confirm significant cell regeneration efficacy compared to 1% FBS for two cell experimental groups.
  • each group was given a lotion prepared in Examples 1 and 2 and Comparative Examples 1 and 2 to apply a certain amount to the eyes around once a week for 12 weeks. After 12 weeks, the skin elasticity of each group was measured using a cutometer (SEM474, Courage + Khazaka electronic GmbH. Germany) that can measure skin elasticity, and the average was obtained. The experimental results are shown in Table 2 below.
  • the cosmetic composition containing the fermented soybean extract fraction according to the present invention improved the skin elasticity, the skin elasticity improved as the content was increased.
  • the cosmetic composition (comparative example 1) containing the general fermented soybean extract and the cosmetic composition (comparative example 2) not containing the fermented soybean extract can be confirmed that the skin elasticity improving effect is low compared to Examples 1 to 2 above. there was.
  • the cosmetic composition (Examples 1 to 2) containing the fermented soybean extract fraction according to the present invention improved the skin wrinkles, the skin wrinkle improvement effect was increased as the content increases.
  • the cosmetic composition containing a general fermented soybean extract (Comparative Example 1) and the cosmetic composition containing no fermented soybean extract (Comparative Example 2) can be confirmed that the skin wrinkle improvement effect is low compared with Examples 1 to 2 above. there was.

Abstract

The present invention relates to a cosmetic composition comprising a fermented soybean extract fraction as an active ingredient, and more specifically relates to a cosmetic composition which has an outstanding cell-growing effect and cell-regenerating effect due to the inclusion of the fermented soybean extract fraction of the present invention and relates to the use of the composition in countering aging.

Description

발효콩 추출 분획물을 유효 성분으로 포함하는 화장료 조성물Cosmetic composition comprising fermented soybean extract fraction as active ingredient
본 발명은 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물에 관한 것으로, 보다 상세하게는 본 발명의 발효콩 추출 분획물을 함유함으로써 세포 증식 효과 및 세포 재생 효과가 우수한 화장료 조성물 및 상기 조성물의 항노화용으로서의 용도에 관한 것이다.The present invention relates to a cosmetic composition containing a fermented soybean extract fraction as an active ingredient, and more particularly, by containing a fermented soybean extract fraction of the present invention excellent in cell proliferation effect and cell regeneration effect and anti-aging of the composition It relates to the use as.
청국장은 콩을 원료로 한 우리나라의 대표적인 발효식품으로서, 간장, 된장, 고추장 등과 함께 오늘날까지 상용되어 온 전통 장류 중의 하나이며, 곡류를 주식으로 하는 우리민족에게 결핍되기 쉬운 필수아미노산 및 지방산을 공급시켜 주는 식품으로서 중요한 역할을 담당하여 왔다. 청국장은 삶은 콩을 볏짚에 붙어있는 고초균(枯草菌)이라 부르는 바실러스 서브틸리스(Bacillus subtilis)를 이용하여 띄워 만든 것으로서, 발효과정 중에 고초균이 생산하는 효소에 의해서 그 특유의 맛과 냄새를 내는 동시에, 원료 콩의 당질과 단백질에서 유래된 레반형프룩탄(levan form fructan)과 폴리글루타메이트(polyglutamate)의 혼합물질인 점질물을 다량 생성하며, 이렇게 발효 제조된 청국장은 소화 작용뿐만 아니라 영양 면에서도 된장이나 고추장보다 단백질과 지방 함량이 높은 양질의 콩 발효식품이다. 청국장 발효는 바실러스 서브틸리스(Bacillus subtilis)에 의해 이루어지는 것으로 알려져 있으며(Lee et al.,J. Micorbiol. Biotechnol, 13, pp. 892-896, 2003), 상기의 바실러스 서브틸리스(Bacillus subtilis)는 장내부패균의 활동을 약화시키고 병원균에 대한 항균 작용뿐만 아니라, 암모니아, 인돌, 아민 등 발암촉진 물질을 감소시키는 작용을 한다. 특히, 바실러스 서브틸리스(Bacillus subtilis)는 비타민 B2를 많이 분비함으로써 간의 해독 기능을 좋게 하여 술, 담배에 대한 뛰어난 해독 효과를 보이며, 유기산의 생성을 통하여 장을 자극하는 등의 반응으로 소화활동 증진 및 면역력을 높여주는 것으로 알려져 있으나 지금까지 발효콩 추출 분획물을 이용하여 세포 재생 화장료 조성물로 개발한 어떠한 내용도 교시되거나 기재된 바 없다.Cheonggukjang is one of Korea's representative fermented foods made from soybean. It is one of the traditional types of soy sauce, soy sauce, soybean paste, and red pepper paste that have been commonly used to this day. The state has played an important role as a food. Cheonggukjang is made by using Bacillus subtilis called Bacillus subtilis, which is attached to rice straw, and gives its unique taste and smell by enzymes produced by Bacillus subtilis during fermentation. In addition, it produces a large amount of viscous substance, which is a mixture of levan form fructan and polyglutamate derived from protein sugar and raw protein soybeans. It is a high quality fermented soybean with higher protein and fat content. Cheonggukjang fermentation is known to be performed by Bacillus subtilis (Lee et al., J. Micorbiol. Biotechnol, 13, pp. 892-896, 2003), and Bacillus subtilis as described above. Decreases the activity of intestinal rot bacteria and acts to reduce carcinogens such as ammonia, indole and amine, as well as antibacterial action against pathogens. In particular, Bacillus subtilis secretes a lot of vitamin B2, improves the liver's detoxification function, shows an excellent detoxification effect on alcohol and tobacco, and stimulates the intestine through the production of organic acids, thereby enhancing digestive activity. And it is known to increase the immunity, but so far has not been taught or described any content developed as a cell regenerative cosmetic composition using the fermented soybean extract fraction.
이에, 본 발명자들은 피부에 안전하면서도 세포 증식 및 세포 재생 효과가 우수하고, 제품으로서의 안정성도 우수한 화장료를 제조하기 위하여 연구하던 중, 발효콩 추출 분획물이 세포 증식 및 세포 재생 효과가 매우 우수한 것을 발견하고 본 발명을 완성하게 되었다.Therefore, the inventors of the present invention while researching to produce a cosmetic that is safe on the skin and has excellent cell proliferation and cell regeneration effects and stability as a product, found that the fermented soybean extract fractions have very excellent cell proliferation and cell regeneration effects. The present invention has been completed.
따라서, 본 발명은 발효콩 추출 분획물을 유효성분으로 함유하여 세포 증식 및 세포 재생 효능이 우수한 화장료 조성물 및 상기 조성물의 항노화용으로서의 용도를 제공하는 것을 목적으로 한다.Therefore, an object of the present invention is to provide a cosmetic composition excellent in cell proliferation and cell regeneration efficacy by containing the fermented soybean extract fraction as an active ingredient and the use as an anti-aging for the composition.
상기한 목적을 달성하기 위하여, 본 발명은 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물을 제공한다.In order to achieve the above object, the present invention provides a cosmetic composition containing a fermented soybean extract fraction as an active ingredient.
또한, 본 발명은 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물의 항노화용으로서의 용도를 제공한다.The present invention also provides a use as an anti-aging cosmetic composition containing a fermented soybean extract fraction as an active ingredient.
본 발명에 따라 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물은 피부 세포 증식 및 세포 재생 효과가 우수하여 상처 회복을 위한 피부 재생 능력을 향상시킬 수 있고, 피부 재생 능력 감퇴로 인한 피부 탄력 감소 및 피부 노화의 심화를 방지함으로써 우수한 피부 탄력 개선 효과 및 피부 노화 방지 효과를 나타낸다.The cosmetic composition containing the fermented soybean extract fraction as an active ingredient according to the present invention is excellent in skin cell proliferation and cell regeneration effect to improve the skin regeneration ability for wound recovery, reduced skin elasticity due to skin regeneration ability decline and By preventing the deepening of skin aging, it shows an excellent skin elasticity improving effect and skin aging effect.
도 1은 발효콩 추출 분획물, 발효콩 추출물의 세포 독성 평가를 측정한 그래프이다.1 is a graph measuring the cytotoxicity evaluation of fermented soybean extract fraction, fermented soybean extract.
도 2는 발효콩 추출 분획물의 인간 섬유 아세포에서의 세포 재생 효과를 나타낸 그래프이다.2 is a graph showing the cell regeneration effect in human fibroblasts of the fermented soybean extract fractions.
도 3은 발효콩 추출 분획물의 HaCaT 세포에서의 세포 재생 효과를 나타낸 그래프이다.Figure 3 is a graph showing the effect of cell regeneration in HaCaT cells of the fermented soybean extract fractions.
본 발명은 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물을 제공한다.The present invention provides a cosmetic composition containing a fermented soybean extract fraction as an active ingredient.
이하, 본 발명을 보다 구체적으로 설명한다.Hereinafter, the present invention will be described in more detail.
일반적인 추출법에 의해 추출한 뒤 여과 및 농축하여 제조되는 발효콩 추출물과는 달리, 본 발명에 따른 발효콩 추출 분획물은 농축된 상태의 발효콩 추출물을 유기용매, 예를 들어 에탄올로 한번 더 추출하여 여과한 후, 막 필터(membrane filter)를 이용한 원심분리를 통해 분리되어 나온 여액을 감압 농축하여 수득할 수 있다.Unlike fermented soybean extract prepared by filtration and concentration after extraction by a general extraction method, the fermented soybean extract fraction according to the present invention is filtered by extracting the fermented soybean extract in a concentrated state once more with an organic solvent, for example ethanol After that, the filtrate separated through centrifugation using a membrane filter may be obtained by concentrating under reduced pressure.
본 발명의 발효콩 추출 분획물은 3kDa~10kDa의 분자량을 갖는 것을 특징으로 한다. 3kDa 미만의 분자량에서는 세포 독성을 나타내기 때문에 바람직하지 않다.Fermented soybean extract fraction of the present invention is characterized by having a molecular weight of 3kDa ~ 10kDa. It is not preferred at molecular weights below 3 kDa because it shows cytotoxicity.
본 발명의 화장료 조성물은 발효콩 추출 분획물을 조성물 총 중량에 대하여 0.0001~30중량%, 보다 바람직하게는 0.1~10중량%의 양으로 함유한다. 그 함량이 0.0001중량% 미만이면 유효성분에 의한 세포 증식 및 세포 재생 효과 등을 얻을 수 없고, 30중량% 초과이면 함량 증가에 비해 효과의 증가가 크지 않기 때문에 바람직하지 않다.The cosmetic composition of the present invention contains the fermented soybean extract fraction in an amount of 0.0001 to 30% by weight, more preferably 0.1 to 10% by weight based on the total weight of the composition. If the content is less than 0.0001% by weight can not obtain the effect of cell proliferation and cell regeneration by the active ingredient, and more than 30% by weight is not preferable because the increase of the effect is not large compared to the increase in content.
본 발명에 의한 발효콩 추출 분획물은 그 사용에 특별히 제한되지 않으며, 예를 들면, 화장료 조성물 이외에도 약제 조성물 등에 사용될 수 있다.Fermented soybean extract fraction according to the present invention is not particularly limited to its use, for example, can be used in pharmaceutical compositions and the like in addition to the cosmetic composition.
본 발명의 화장료 조성물의 제형은 특별히 제한되지 않으며, 목적하는 바에 따라 적절히 선택할 수 있다. 예를 들어, 본 발명의 화장료 조성물은 유연화장수, 영양화장수, 마사지크림, 에센스, 영양크림, 팩, 피부외용연고 등의 제형으로 제조될 수 있다.The formulation of the cosmetic composition of the present invention is not particularly limited and may be appropriately selected as desired. For example, the cosmetic composition of the present invention may be prepared in a formulation such as flexible cosmetics, nourishing cosmetics, massage creams, essences, nutrition creams, packs, skin ointments.
이하, 본 발명을 실시예 및 시험예를 들어 보다 상세히 설명하겠지만, 본 발명이 이들 예로만 한정되는 것은 아니다. Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited only to these examples.
[제조예 1] 발효 청국장 추출물의 제조Preparation Example 1 Preparation of Fermented Cheonggukjang Extract
발효 청국장 1kg에 물 5ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압 농축하여 발효 청국장 추출물 350g을 얻었다.5 L of water was added to 1 kg of fermented Cheonggukjang and refluxed three times, followed by immersion at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 350 g of fermented Cheonggukjang extract.
[제조예 2] 발효 청국장 추출 분획물의 제조Preparation Example 2 Preparation of Fermented Cheonggukjang Extract Fraction
상기 제조예 1의 발효 청국장 추출물 100g을 EtOH 3ℓ에 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 UF(Ultra-Filtration) 키트에 넣은 뒤 원심분리를 통해 3kDa 이상의 분자량을 가지는 샘플로 분리한 뒤 분리된 여액을 감압 농축하여 발효 청국장 추출 분획물 33g을 얻었다.100 g of the fermented Cheonggukjang extract of Preparation Example 1 was placed in 3 l of EtOH, extracted under reflux three times, and then deposited at 15 ° C. for 1 day. After that, the residue and the filtrate are separated by filtration and centrifugation, and the filtrate is separated into a UF (Ultra-Filtration) kit, and then separated by a centrifugation into a sample having a molecular weight of 3 kDa or more, and the filtrate is separated under reduced pressure. Concentration gave 33 g of fermented Chunggukjang extract fractions.
[시험예 1] 세포 독성 평가(MTT assay)Test Example 1 Cytotoxicity Evaluation (MTT assay)
시험 물질의 세포 독성을 평가하기 위해 HaCaT 10000 세포수/웰을 96-웰 플레이트에 심었다. 24시간 배양 후 FBS가 들어있지 않은 배지에서 24시간 배양하였다. 배양 후 배지를 제거하고 PBS를 100㎕씩 넣어준 후에 UVB 40mJ을 조사하였다. 이때 UVB를 조사하지 않는 실험군도 만들었다. 물질을 농도별로 처리하여 48시간 동안 배양 후, MTT 용액(solution)(0.5mg/mL)을 100㎕씩 넣어주고 3시간 동안 37℃의 배양기에서 배양하였다. 상기 용액을 제거하면 포르마잔(formazan)이 형성되는데 DMSO를 100㎕ 넣어 주고 녹인 후, 570nm에서의 흡광도를 측정하여 세포독성을 확인하였다. 그 결과는 도 1에 나타내었다.HaCaT 10000 cell counts / well were planted in 96-well plates to assess the cytotoxicity of the test substance. After 24 hours of incubation was incubated for 24 hours in a medium containing no FBS. After incubation, the medium was removed, 100 μl of PBS was added, and UVB 40mJ was irradiated. At this time, the experimental group that does not irradiate UVB was also made. The material was treated by concentration and incubated for 48 hours, and then 100 μl of MTT solution (0.5mg / mL) was added thereto and incubated in an incubator at 37 ° C. for 3 hours. When the solution was removed, formazan was formed. 100 μl of DMSO was added and dissolved, and then cytotoxicity was confirmed by measuring absorbance at 570 nm. The results are shown in FIG.
도 1로부터 알 수 있듯이, 본 발명에 따른 발효콩 추출 분획물은 세포 독성이 적으면서 세포 증식 효능이 우수함을 확인할 수 있었다. 반면, 발효콩 추출물은 발효콩 추출 분획물과 비교하여 세포 독성이 크며, 농도에 비례하여 세포 독성도 증가함을 알 수 있었다.As can be seen from Figure 1, the fermented soybean extract fraction according to the present invention was confirmed that the cell proliferation efficiency while being less cytotoxic. On the other hand, the fermented soybean extract has a greater cytotoxicity compared to the fermented soybean extract fraction, and it was found that the cytotoxicity increased in proportion to the concentration.
[시험예 2] 세포 재생 평가(Cell Proliferation assay) Test Example 2 Cell Proliferation Assay
시험 물질의 세포 재생 효과를 알아보기 위해 HaCaT 10000세포수/웰, 정상 인간 섬유아세포(normal human fibroblast) 6000 세포수/웰로　96-웰 플레이트에 심었다. 물질을 처리하기 전에 배지를 FBS가 첨가되지 않은 배지로 교환하고 1시간 동안 배양하였다. 이후 배지를 제거하고 FBS가 1% 함유된 배지에 상기 제조예 2의 발효 청국장 추출 분획물을 0.1, 1, 5, 10, 50, 100ppm의 농도별로 녹인 후 처리하였다. 이때 10% FBS가 함유된 배지는 양성대조군으로 사용하였다. 상기 제조예 2의 발효 청국장 추출 분획물 처리 후 48시간 동안 배양 후, WST-1을 100㎕/웰로 처리하였다. WST-1을 처리하고 3시간 동안 37℃ 5% CO2배양기에서 반응시켰다. 3시간 동안 배양 후 마이크로플레이트 리더기(microplate reader)를 이용하여 450nm에서 흡광도를 측정하여 세포 재생 효과를 확인하였다. 그 결과는 도 2 및 3에 나타내었다. In order to determine the cell regeneration effect of the test substance was planted in 96-well plate at HaCaT 10000 cells / well, normal human fibroblast 6000 cells / well. The medium was replaced with medium without FBS and incubated for 1 hour before the material was treated. Thereafter, the medium was removed and the fermented Cheonggukjang extract fraction of Preparation Example 2 was dissolved in a concentration of 0.1, 1, 5, 10, 50, and 100 ppm in a medium containing 1% of FBS, followed by treatment. At this time, the medium containing 10% FBS was used as a positive control. After incubation for 48 hours after the fermented Cheonggukjang extract fraction of Preparation Example 2, WST-1 was treated with 100 μl / well. WST-1 was treated and reacted in a 37 ° C. 5% CO 2 incubator for 3 hours. After incubation for 3 hours, the absorbance was measured at 450 nm using a microplate reader to confirm cell regeneration effect. The results are shown in FIGS. 2 and 3.
도 2 및 3으로부터 본 발명에 따른 발효콩 추출 분획물은 두 가지 세포 실험군에 대해 1% FBS 대비 유의적인 세포 재생 효능을 확인할 수 있었다.Fermented soybean extract fraction according to the present invention from Figures 2 and 3 was able to confirm significant cell regeneration efficacy compared to 1% FBS for two cell experimental groups.
[시험예 3] 피부 탄력 개선 효과Test Example 3 Skin Elasticity Improvement Effect
하기 표 1의 조성을 갖는 실시예 1~2 및 비교예 1~2의 화장수 제형을 제조하였다.To the cosmetic formulations of Examples 1 and 2 and Comparative Examples 1 and 2 having the composition of Table 1 below.
표 1
성분명 실시예 1 실시예 2 비교예 1 비교예 2
발효 청국장 추출 분획물(상기 제조예 2) 0.5 10 - -
발효 청국장 추출물(상기 제조예 1) - - 0.5 -
세테아릴글루코사이드 1.5 1.5 1.5 1.5
세틸옥타노에이트 3.0 3.0 3.0 3.0
메틸파라벤 0.15 0.15 0.15 0.15
에칠렌디아민테트라초산디나트륨 0.02 0.02 0.02 0.02
정제수 to 100 to 100 to 100 to 100
Table 1
Ingredient Name Example 1 Example 2 Comparative Example 1 Comparative Example 2
Fermented Cheonggukjang Extract Fraction (Preparation Example 2) 0.5 10 - -
Fermented Cheonggukjang Extract (Preparation Example 1) - - 0.5 -
Cetearylglucoside 1.5 1.5 1.5 1.5
Cetyloctanoate 3.0 3.0 3.0 3.0
Methylparaben 0.15 0.15 0.15 0.15
Ethylenediaminetetradiacetic acid sodium 0.02 0.02 0.02 0.02
Purified water to 100 to 100 to 100 to 100
상기 실시예 1~2 및 비교예 1~2에서 제조된 화장수에 대하여 피부 탄력 향상 효과를 비교하기 위하여, 30∼40대 여성 80명을 각각 20명씩 4개군으로 나누어 측정하였다. 실험 방법은 다음과 같다.In order to compare the skin elasticity improvement effect on the lotion prepared in Examples 1 and 2 and Comparative Examples 1 and 2, 80 women in their 30s and 40s were measured by dividing into four groups of 20 people each. The experimental method is as follows.
먼저, 각 군에 실시예 1~2 및 비교예 1~2에서 제조된 화장수를 주고 매일 1회 12주간 눈가에 일정량을 도포하게 하였다. 12주 뒤 피부탄력을 측정할 수 있는 큐토미터(cutometer; SEM474, Courage+Khazaka electronic GmbH. Germany)를 이용하여 각 군의 피부 탄력을 측정하고 그 평균을 구하였다. 그 실험 결과는 하기 표 2에 나타내었다.First, each group was given a lotion prepared in Examples 1 and 2 and Comparative Examples 1 and 2 to apply a certain amount to the eyes around once a week for 12 weeks. After 12 weeks, the skin elasticity of each group was measured using a cutometer (SEM474, Courage + Khazaka electronic GmbH. Germany) that can measure skin elasticity, and the average was obtained. The experimental results are shown in Table 2 below.
표 2
구분 피부탄력향상도(%)
실시예 1 26.8
실시예 2 33.6
비교예 1 1.1
비교예 2 0.4
TABLE 2
division Skin elasticity improvement (%)
Example 1 26.8
Example 2 33.6
Comparative Example 1 1.1
Comparative Example 2 0.4
상기 표 2에서 알 수 있듯이, 본 발명에 따른 발효콩 추출 분획물을 함유하는 화장료 조성물(실시예 1~2)은 피부 탄력을 향상시켰으며, 함량이 증가함에 따라 피부 탄력 향상도가 높아졌다. 반면, 일반적인 발효콩 추출물을 함유하는 화장료 조성물(비교예 1) 및 발효콩 추출물을 함유하지 않는 화장료 조성물(비교예 2)는 상기 실시예 1~2와 비교하여 피부 탄력 향상 효과가 낮음을 확인할 수 있었다.As can be seen in Table 2, the cosmetic composition containing the fermented soybean extract fraction according to the present invention (Examples 1 to 2) improved the skin elasticity, the skin elasticity improved as the content was increased. On the other hand, the cosmetic composition (comparative example 1) containing the general fermented soybean extract and the cosmetic composition (comparative example 2) not containing the fermented soybean extract can be confirmed that the skin elasticity improving effect is low compared to Examples 1 to 2 above. there was.
[시험예 4] 피부 주름 개선 효과Test Example 4 Skin Wrinkle Improvement
상기 실시예 1~2 및 비교예 1~2의 피부 주름 개선 효과를 확인하기 위하여 다음과 같이 실험을 실시하였다.In order to confirm the skin wrinkle improvement effect of Examples 1 and 2 and Comparative Examples 1 and 2 were carried out as follows.
30대 여성 80명을 각각 실시예 1~2 및 비교예 1~2군으로 20명씩 4개군으로 나누어 매일 1회 8주간 도포한 후, 실리콘을 이용하여 레플리카를 떠서 주름의 상태를 비지오미터(visiometer)로 측정하여 화상분석하고, 그 결과를 하기 표 3에 나타내었다. 표 3의 결과는 도포 8주 후의 각각의 파라미터(parameter) 값에서 도포 전 파라미터 값을 뺀 것의 평균이다.80 women in their 30s were divided into 4 groups of 20 people in Examples 1 and 2 and Comparative Examples 1 and 2, respectively, and applied once a day for 8 weeks, and then, using a silicone, the replica was scooped up to measure the state of wrinkles. Image analysis was carried out by measuring), and the results are shown in Table 3 below. The results in Table 3 are the average of each parameter value after 8 weeks of application minus the parameter value before application.
표 3
사용 8주 후임상 결과 R1 R2 R3 R4 R5
실시예 1 -0.18 -0.14 -0.08 -0.06 -0.05
실시예 2 -0.27 -0.20 -0.10 -0.08 -0.06
비교예 1 -0.08 -0.07 -0.03 -0.02 -0.03
비교예 2 0.03 0.05 0.02 -0.01 0.01
R1: 주름 등고선의 최고치와 최저치의 차이값R2: 주름 등고선을 임의로 5칸씩 나눈 후 그 중 R1값 들의 평균R3: 5개씩 나눈 R1값 중 최고치R4: 주름 등고선의 baseline에서 각 각의 꼭대기와 계곡의 값을 뺀 평균값R5: 주름 등고선의 baseline에서 각 각의 주름 윤곽을 뺀 값의 차이 값
TABLE 3
Clinical results after 8 weeks of use R1 R2 R3 R4 R5
Example 1 -0.18 -0.14 -0.08 -0.06 -0.05
Example 2 -0.27 -0.20 -0.10 -0.08 -0.06
Comparative Example 1 -0.08 -0.07 -0.03 -0.02 -0.03
Comparative Example 2 0.03 0.05 0.02 -0.01 0.01
R1: Difference between the maximum and minimum values of the wrinkle contours R2: Randomly divide the five wrinkled contours by five spaces, then average R3 of the R1 values divided by five R4: The highest value of the R1 values divided by five R4: At the baseline of the wrinkled contours Average minus value R5: Difference value of the baseline of the wrinkle contour minus each wrinkle outline
상기 표 3에서 알 수 있듯이, 본 발명에 따른 발효콩 추출 분획물을 함유하는 화장료 조성물(실시예 1~2)은 피부 주름을 개선시켰으며, 함량이 증가함에 따라 피부 주름 개선 효과가 높아졌다. 반면, 일반적인 발효콩 추출물을 함유하는 화장료 조성물(비교예 1) 및 발효콩 추출물을 함유하지 않는 화장료 조성물(비교예 2)는 상기 실시예 1~2와 비교하여 피부 주름 개선 효과가 낮음을 확인할 수 있었다.As can be seen in Table 3, the cosmetic composition (Examples 1 to 2) containing the fermented soybean extract fraction according to the present invention improved the skin wrinkles, the skin wrinkle improvement effect was increased as the content increases. On the other hand, the cosmetic composition containing a general fermented soybean extract (Comparative Example 1) and the cosmetic composition containing no fermented soybean extract (Comparative Example 2) can be confirmed that the skin wrinkle improvement effect is low compared with Examples 1 to 2 above. there was.
하기에 상기 조성물의 제형예들을 설명하나, 본 발명은 이를 한정하고자 함이 아니라 단지 구체적으로 설명하고자 함이다. Examples of the formulations of the composition are described below, but the present invention is not intended to be limited thereto but merely to be described in detail.
[제형예 1] 유연화장수Formulation Example 1 Flexible Cosmetics
표 4
성분명 함량(중량%)
발효콩 추출 분획물 0.5
부틸렌글리콜 2.0
프로필렌글리콜 2.0
카르복시비닐폴리머 0.1
피이지-12 노닐페닐에테르 0.2
폴리솔베이트 80 0.4
에탄올 10.0
트리에탄올아민 0.1
방부제, 색소 및 향료 적량
정제수 to 100
Table 4
Ingredient Name Content (% by weight)
Fermented Soybean Extract Fraction 0.5
Butylene glycol 2.0
Propylene glycol 2.0
Carboxy Vinyl Polymer 0.1
Fiji-12 nonylphenyl ether 0.2
Polysorbate 80 0.4
ethanol 10.0
Triethanolamine 0.1
Preservatives, Colors and Flavors Quantity
Purified water to 100
[제형예 2] 영양화장수Formulation Example 2 Nutritional Cosmetics
표 5
성분명 함량(중량%)
발효콩 추출 분획물 0.5
밀납 4.0
폴리솔베이트 60 1.5
솔비탄세스퀴올레이트 1.5
유동파라핀 0.5
카프릴릭/카프릭트리글리세라이드 5.0
글리세린 3.0
부틸렌글리콜 3.0
프로필렌글리콜 3.0
카르복시비닐폴리머 0.1
트리에탄올아민 0.2
방부제, 색소 및 향료 적량
정제수 to 100
Table 5
Ingredient Name Content (% by weight)
Fermented Soybean Extract Fraction 0.5
Beeswax 4.0
Polysorbate 60 1.5
Sorbitan sesquioleate 1.5
Liquid paraffin 0.5
Caprylic / Capric Triglycerides 5.0
glycerin 3.0
Butylene glycol 3.0
Propylene glycol 3.0
Carboxy Vinyl Polymer 0.1
Triethanolamine 0.2
Preservatives, Colors and Flavors Quantity
Purified water to 100
[제형예 3] 영양크림Formulation Example 3 Nutrition Cream
표 6
성분명 함량(중량%)
발효콩 추출 분획물 0.5
밀납 10.0
폴리솔베이트 60 1.5
피이지 60 경화피마자유 2.0
솔비탄세스퀴올레이트 0.5
유동파라핀 10.0
스쿠알란 5.0
카프릴릭/카프릭트리글리세라이드 5.0
글리세린 5.0
부틸렌글리콜 3.0
프로필렌글리콜 3.0
트리에탄올아민 0.2
방부제, 색소 및 향료 적량
정제수 to 100
Table 6
Ingredient Name Content (% by weight)
Fermented Soybean Extract Fraction 0.5
Beeswax 10.0
Polysorbate 60 1.5
Sebum 60 Cured Castor Oil 2.0
Sorbitan sesquioleate 0.5
Liquid paraffin 10.0
Squalane 5.0
Caprylic / Capric Triglycerides 5.0
glycerin 5.0
Butylene glycol 3.0
Propylene glycol 3.0
Triethanolamine 0.2
Preservatives, Colors and Flavors Quantity
Purified water to 100
[제형예 4] 마사지크림Formulation Example 4 Massage Cream
표 7
성분명 함량(중량%)
발효콩 추출 분획물 0.5
밀납 10.0
폴리솔베이트 60 1.5
피이지 60 경화피마자유 2.0
솔비탄세스퀴올레이트 0.8
유동파라핀 40.0
스쿠알란 5.0
카프릴릭/카프릭트리글리세라이드 4.0
글리세린 5.0
부틸렌글리콜 3.0
프로필렌글리콜 3.0
트리에탄올아민 0.2
방부제, 색소 및 향료 적량
정제수 to 100
TABLE 7
Ingredient Name Content (% by weight)
Fermented Soybean Extract Fraction 0.5
Beeswax 10.0
Polysorbate 60 1.5
Sebum 60 Cured Castor Oil 2.0
Sorbitan sesquioleate 0.8
Liquid paraffin 40.0
Squalane 5.0
Caprylic / Capric Triglycerides 4.0
glycerin 5.0
Butylene glycol 3.0
Propylene glycol 3.0
Triethanolamine 0.2
Preservatives, Colors and Flavors Quantity
Purified water to 100
[제형예 5] 팩[Formulation Example 5] Pack
표 8
성분명 함량(중량%)
발효콩 추출 분획물 0.5
폴리비닐알콜 13.0
소듐카르복시메틸셀룰로오스 0.2
글리세린 5.0
알란토인 0.1
에탄올 6.0
핑지-12 노닐페닐에테르 0.3
폴리솔베이트 60 0.3
방부제, 색소 및 향료 적량
정제수 to 100
Table 8
Ingredient Name Content (% by weight)
Fermented Soybean Extract Fraction 0.5
Polyvinyl alcohol 13.0
Sodium Carboxymethyl Cellulose 0.2
glycerin 5.0
Allantoin 0.1
ethanol 6.0
Pingji-12 Nonylphenyl Ether 0.3
Polysorbate 60 0.3
Preservatives, Colors and Flavors Quantity
Purified water to 100

Claims (6)

  1. 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물.Cosmetic composition containing a fermented soybean extract fraction as an active ingredient.
  2. 제 1항에 있어서, 상기 발효콩 추출 분획물을 조성물 총 중량에 대하여 0.0001~30중량%의 양으로 함유하는 것을 특징으로 하는 화장료 조성물.The cosmetic composition according to claim 1, wherein the fermented soybean extract fraction is contained in an amount of 0.0001 to 30% by weight based on the total weight of the composition.
  3. 제 1항에 있어서, 발효콩 추출 분획물은 3kDa~10kDa의 분자량을 갖는 것을 특징으로 하는 화장료 조성물.The cosmetic composition according to claim 1, wherein the fermented soybean extract fraction has a molecular weight of 3kDa-10kDa.
  4. 제 1항에 있어서, 상기 조성물이 세포 증식 효과가 우수한 것을 특징으로 하는 화장료 조성물.The cosmetic composition according to claim 1, wherein the composition has an excellent cell proliferation effect.
  5. 제 1항에 있어서, 상기 조성물이 세포 재생 효과가 우수한 것을 특징으로 하는 화장료 조성물.The cosmetic composition according to claim 1, wherein the composition has excellent cell regeneration effect.
  6. 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물의 항노화용으로서의 용도.Use as anti-aging cosmetic composition containing fermented soybean extract fraction as an active ingredient.
PCT/KR2011/006657 2010-09-13 2011-09-08 Cosmetic composition comprising a fermented soybean extract fraction as an active ingredient WO2012036414A2 (en)

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CN110755472A (en) * 2019-09-24 2020-02-07 萧敬皇 Soybean fermentation composition for skin wound healing and preparation method thereof

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KR101985356B1 (en) * 2012-08-03 2019-06-04 (주)아모레퍼시픽 Composition for skin external application containing fermented soybean extract
KR102117218B1 (en) * 2018-01-18 2020-06-01 코스맥스 주식회사 Composition for improving skin beauty comprising extract of fermented soybean fermented with Aspergillus cristatus strain
KR102488533B1 (en) * 2020-12-24 2023-01-16 에이앤펩주식회사 Preparing method of highly functional anti-inflammatory component drived from Glycine max Merr. fermentation extracts and cosmetic composition using the same

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KR20030089710A (en) * 2001-04-06 2003-11-22 가부시키가이샤 도요학코 Cosmetic materials and process for producing the same
JP2005512988A (en) * 2001-11-09 2005-05-12 コグニス・フランス・ソシエテ・アノニム Use of extracts from bigna aconitifolia plants in cosmetic and / or dermopharmaceutical compositions
KR100640246B1 (en) * 2004-12-27 2006-11-01 정경식 Fermented product, new compound obtained therefrom, and skin whitening products containing the same
KR100723844B1 (en) * 2005-07-11 2007-05-31 조정일 Health care and skin care bath and beauty material using fermented soybean extract

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KR20030089710A (en) * 2001-04-06 2003-11-22 가부시키가이샤 도요학코 Cosmetic materials and process for producing the same
JP2005512988A (en) * 2001-11-09 2005-05-12 コグニス・フランス・ソシエテ・アノニム Use of extracts from bigna aconitifolia plants in cosmetic and / or dermopharmaceutical compositions
KR100640246B1 (en) * 2004-12-27 2006-11-01 정경식 Fermented product, new compound obtained therefrom, and skin whitening products containing the same
KR100723844B1 (en) * 2005-07-11 2007-05-31 조정일 Health care and skin care bath and beauty material using fermented soybean extract

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110755472A (en) * 2019-09-24 2020-02-07 萧敬皇 Soybean fermentation composition for skin wound healing and preparation method thereof

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