WO2012033382A3 - 신규한 ｒｎａ 결합 단백질 및 이를 이용한 무표지 마이크로 ｒｎａ 검출방법 - Google Patents

신규한 ｒｎａ 결합 단백질 및 이를 이용한 무표지 마이크로 ｒｎａ 검출방법 Download PDF

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WO2012033382A3
WO2012033382A3 PCT/KR2011/006710 KR2011006710W WO2012033382A3 WO 2012033382 A3 WO2012033382 A3 WO 2012033382A3 KR 2011006710 W KR2011006710 W KR 2011006710W WO 2012033382 A3 WO2012033382 A3 WO 2012033382A3
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microrna
rna
label
binding protein
same
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PCT/KR2011/006710
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French (fr)
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WO2012033382A2 (ko
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정용원
정봉현
이정민
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한국생명공학연구원
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Publication of WO2012033382A2 publication Critical patent/WO2012033382A2/ko
Publication of WO2012033382A3 publication Critical patent/WO2012033382A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Plant Pathology (AREA)

Abstract

본 발명은 신규한 RNA 결합 단백질, 이를 포함하는 마이크로 RNA 검출용 조성물, 및 이를 이용하여 무표지 마이크로 RNA를 검출하는 방법에 관한 것이다. 본 발명의 파즈 도메인 및 이중가닥 RNA 결합 도메인을 포함하는 융합 단백질로서 3'말단이 돌출된 이중가닥 RNA 에 결합하는 RNA 결합 단백질을 이용하여 마이크로 RNA 를 검출하는 방법은, 효소적 표지과정 또는 증폭반응 없이 마이크로 RNA를 탐지하는 무표지 마이크로 RNA 검출 방법으로서, 기존의 마이크로 RNA 분석방법, 즉 효소적 방법으로 표지 후 분석하는 방법 또는 항체를 마이크로 RNA 에 결합하는 방법에 있어서, 공정이 복잡한 단점 또는 불안정한 RNA 구조로 인해 RNA 구조에 특이적인 항체의 개발이 어려운 단점을 해결할 수 있다. 이는 다양한 질환에서 유전자의 발현연구, 질환의 진단마커의 탐색 및 약물의 효율성 평가에 활용할 수 있는 등의 다양한 가능성을 갖는다.
PCT/KR2011/006710 2010-09-10 2011-09-09 신규한 ｒｎａ 결합 단백질 및 이를 이용한 무표지 마이크로 ｒｎａ 검출방법 WO2012033382A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0089137 2010-09-10
KR1020100089137A KR101297830B1 (ko) 2010-09-10 2010-09-10 신규한 ｒｎａ 결합 단백질 및 이를 이용한 무표지 마이크로 ｒｎａ 검출방법

Publications (2)

Publication Number Publication Date
WO2012033382A2 WO2012033382A2 (ko) 2012-03-15
WO2012033382A3 true WO2012033382A3 (ko) 2012-06-28

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Application Number Title Priority Date Filing Date
PCT/KR2011/006710 WO2012033382A2 (ko) 2010-09-10 2011-09-09 신규한 ｒｎａ 결합 단백질 및 이를 이용한 무표지 마이크로 ｒｎａ 검출방법

Country Status (2)

Country Link
KR (1) KR101297830B1 (ko)
WO (1) WO2012033382A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11401514B2 (en) 2016-07-19 2022-08-02 Brandeis University Compositions and methods for identifying RNA binding polypeptide targets
CA3115864A1 (en) * 2018-10-12 2020-04-16 Peking University Methods and compositions for editing rnas
KR102197368B1 (ko) * 2019-04-01 2020-12-31 서울대학교산학협력단 Risc를 이용한 폴리뉴클레오타이드의 검출방법

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JI, X.: "The mechanism of RNase III action: how dicer dices.", CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY., vol. 320, 2008, pages 99 - 116 *
MACRAE, 1. J. ET AL.: "Ribonuclease revisited: structural insights into ribonuclease III family enzymes.", CURRENT OPINION IN STRUCTURAL BIOLOGY., vol. 17, no. 1, February 2007 (2007-02-01), pages 138 - 45 *
MACRAE, 1. J. ET AL.: "Structure of Dicer and mechanistic implications for RNAi.", COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY., vol. 71, 2006, pages 73 - 80 *
MYERS, J. W. ET AL.: "Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing.", NATURE BIOTECHNOLOGY, vol. 21, no. 3, March 2003 (2003-03-01), pages 324 - 8 *
VERMEULEN, A. ET AL.: "The contributions of dsRNA structure to Dicer specificity and efficiency.", RNA., vol. 11, no. 5, May 2005 (2005-05-01), pages 674 - 682 *

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Publication number Publication date
WO2012033382A2 (ko) 2012-03-15
KR101297830B1 (ko) 2013-08-19
KR20120026927A (ko) 2012-03-20

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