WO2012031910A3 - Method for preparing alpha-ketopimelic acid by c1-elongation - Google Patents

Method for preparing alpha-ketopimelic acid by c1-elongation Download PDF

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Publication number
WO2012031910A3
WO2012031910A3 PCT/EP2011/064718 EP2011064718W WO2012031910A3 WO 2012031910 A3 WO2012031910 A3 WO 2012031910A3 EP 2011064718 W EP2011064718 W EP 2011064718W WO 2012031910 A3 WO2012031910 A3 WO 2012031910A3
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WIPO (PCT)
Prior art keywords
homo
alpha
enzyme
acid
activity
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PCT/EP2011/064718
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French (fr)
Other versions
WO2012031910A2 (en
Inventor
Axel Christoph Trefzer
Stefanus Cornelis Hendrikus Jozef Turk
Original Assignee
Dsm Ip Assets B.V.
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Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2012031910A2 publication Critical patent/WO2012031910A2/en
Publication of WO2012031910A3 publication Critical patent/WO2012031910A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/50—Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01087—Homoisocitrate dehydrogenase (1.1.1.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01114—Methanogen homoaconitase (4.2.1.114)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to a method for preparing alpha-ketopimelic acid, comprising converting alpha-ketoglutaric acid into alpha-ketoadipic acid and converting alpha-ketoadipic acid into alpha-ketopimelic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst catalysing at least one of these conversions, wherein the heterologous biocatalyst comprises a. an NifV enzyme or another Aks enzyme having homo(n)citrate activity or a homologue thereof having homo(n)citrate activity; b. an AksD enzyme having homon-aconitase activity or a homologue thereof having homon-aconitase activity, c. an AksE enzyme having homon-aconitase activity or a homologue thereof having homon-aconitase activity, d. an AksF enzyme having homon- isocitrate dehydrogenase or a homologue thereof having homon-aconitase activity.
PCT/EP2011/064718 2010-09-10 2011-08-26 Method for preparing alpha-ketopimelic acid by c1-elongation WO2012031910A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10176270.6 2010-09-10
EP10176270 2010-09-10

Publications (2)

Publication Number Publication Date
WO2012031910A2 WO2012031910A2 (en) 2012-03-15
WO2012031910A3 true WO2012031910A3 (en) 2012-06-07

Family

ID=43530673

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/064718 WO2012031910A2 (en) 2010-09-10 2011-08-26 Method for preparing alpha-ketopimelic acid by c1-elongation

Country Status (2)

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TW (1) TW201217536A (en)
WO (1) WO2012031910A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103937841A (en) * 2014-05-13 2014-07-23 上海交通大学 Application of enoyl coenzyme A hydratase in adipic acid biosynthesis

Families Citing this family (19)

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Publication number Priority date Publication date Assignee Title
WO2012174451A1 (en) 2011-06-17 2012-12-20 Invista Technologies S.A.R.L. Use of hydrolases to increase monomer content in waste stream
WO2013003744A2 (en) * 2011-06-30 2013-01-03 Invista Techonologies S.A R.L Bioconversion process for producing nylon-7, nylon-7,7 and polyesters
US9102960B2 (en) 2011-12-16 2015-08-11 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
US9102958B2 (en) 2011-12-16 2015-08-11 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
EP2794897A2 (en) * 2011-12-21 2014-10-29 Invista Technologies S.a r.l. Bioconversion process for producing nylon-7, nylon-7,7 and polyesters
WO2014093847A2 (en) 2012-12-14 2014-06-19 Invista North America S.A.R.L. METHODS OF PRODUCING 7-CARBON CHEMICALS VIA CoA-DEPENDENT CARBON CHAIN ELONGATION ASSOCIATED WITH CARBON STORAGE
WO2014105788A2 (en) 2012-12-31 2014-07-03 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via carbon chain elongation associated with cyclohexane carboxylate synthesis
WO2014105796A2 (en) 2012-12-31 2014-07-03 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via aromatic compounds
CN105408487A (en) 2012-12-31 2016-03-16 英威达技术有限责任公司 Methods of producing 7-carbon chemicals via methyl-ester shielded carbon chain elongation
CN105026569A (en) 2012-12-31 2015-11-04 英威达技术有限责任公司 Methods of producing 7-carbon chemicals via pyruvate and succinate semialdehyde aldol condensation
US9580731B2 (en) 2012-12-31 2017-02-28 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via c1 carbon chain elongation associated with coenzyme B synthesis
WO2014105805A2 (en) 2012-12-31 2014-07-03 Invista North America S.A.R.L. Methods of producing 6-carbon chemicals via methyl-ester shielded carbon chain elongation
US9920336B2 (en) 2012-12-31 2018-03-20 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals from long chain fatty acids via oxidative cleavage
US9745607B2 (en) 2014-05-15 2017-08-29 Invista North America S.A.R.L. Methods of producing 6-carbon chemicals using 2,6-diaminopimelate as precursor to 2-aminopimelate
WO2015195707A1 (en) 2014-06-16 2015-12-23 Invista Technologies S.A.R.L. Methods, reagents and cells for biosynthesizing compounds
CN106795530A (en) 2014-06-16 2017-05-31 英威达技术有限责任公司 Method, reagent and cell for biosynthesis compound
EP3155112A1 (en) 2014-06-16 2017-04-19 Invista Technologies S.à.r.l. Process for producing glutarate and glutaric acid methyl ester
WO2015195678A1 (en) 2014-06-16 2015-12-23 Invista Technologies S.À R.L. Methods, reagents and cells for biosynthesizing compounds
CN109337938B (en) * 2018-08-31 2021-05-07 河南师范大学 Method for producing 2-oxoadipic acid by fermenting and degrading paraffin with alcanivorax

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WO2009113855A2 (en) * 2008-03-11 2009-09-17 Dsm Ip Assets B.V. PREPARATION OF 6-AMINOCAPROIC ACID FROM α-KETOPIMELIC ACID
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103937841A (en) * 2014-05-13 2014-07-23 上海交通大学 Application of enoyl coenzyme A hydratase in adipic acid biosynthesis
CN103937841B (en) * 2014-05-13 2015-10-28 上海交通大学 The application of enoyl-CoA hydratase in hexanodioic acid biosynthesizing

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WO2012031910A2 (en) 2012-03-15

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