WO2012031910A3 - Method for preparing alpha-ketopimelic acid by c1-elongation - Google Patents
Method for preparing alpha-ketopimelic acid by c1-elongation Download PDFInfo
- Publication number
- WO2012031910A3 WO2012031910A3 PCT/EP2011/064718 EP2011064718W WO2012031910A3 WO 2012031910 A3 WO2012031910 A3 WO 2012031910A3 EP 2011064718 W EP2011064718 W EP 2011064718W WO 2012031910 A3 WO2012031910 A3 WO 2012031910A3
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- Prior art keywords
- homo
- alpha
- enzyme
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/001—Amines; Imines
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/10—Nitrogen as only ring hetero atom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/50—Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01087—Homoisocitrate dehydrogenase (1.1.1.87)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01114—Methanogen homoaconitase (4.2.1.114)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention relates to a method for preparing alpha-ketopimelic acid, comprising converting alpha-ketoglutaric acid into alpha-ketoadipic acid and converting alpha-ketoadipic acid into alpha-ketopimelic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst catalysing at least one of these conversions, wherein the heterologous biocatalyst comprises a. an NifV enzyme or another Aks enzyme having homo(n)citrate activity or a homologue thereof having homo(n)citrate activity; b. an AksD enzyme having homon-aconitase activity or a homologue thereof having homon-aconitase activity, c. an AksE enzyme having homon-aconitase activity or a homologue thereof having homon-aconitase activity, d. an AksF enzyme having homon- isocitrate dehydrogenase or a homologue thereof having homon-aconitase activity.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10176270 | 2010-09-10 | ||
EP10176270.6 | 2010-09-10 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2012031910A2 WO2012031910A2 (en) | 2012-03-15 |
WO2012031910A3 true WO2012031910A3 (en) | 2012-06-07 |
Family
ID=43530673
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2011/064718 WO2012031910A2 (en) | 2010-09-10 | 2011-08-26 | Method for preparing alpha-ketopimelic acid by c1-elongation |
Country Status (2)
Country | Link |
---|---|
TW (1) | TW201217536A (en) |
WO (1) | WO2012031910A2 (en) |
Cited By (1)
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CN103937841A (en) * | 2014-05-13 | 2014-07-23 | 上海交通大学 | Application of enoyl coenzyme A hydratase in adipic acid biosynthesis |
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MY166083A (en) | 2011-06-17 | 2018-05-24 | Invista Tech Sarl | Use of hydrolases to increase monomer content in waste stream |
JP2014525741A (en) | 2011-06-30 | 2014-10-02 | インビスタ テクノロジーズ エス.アー.エール.エル. | Biotransformation process for producing nylon-7, nylon-7,7, and polyester |
US9102960B2 (en) | 2011-12-16 | 2015-08-11 | Invista North America S.á.r.l. | Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage |
US9102958B2 (en) | 2011-12-16 | 2015-08-11 | Invista North America S.á.r.l. | Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage |
JP2015500663A (en) * | 2011-12-21 | 2015-01-08 | インビスタ テクノロジーズ エス.アー.エール.エル. | Biotransformation process for producing nylon-7, nylon-7,7, and polyester |
US9790525B2 (en) | 2012-12-14 | 2017-10-17 | Invista North America S.A.R.L. | Methods of producing 7-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage |
WO2014105805A2 (en) | 2012-12-31 | 2014-07-03 | Invista North America S.A.R.L. | Methods of producing 6-carbon chemicals via methyl-ester shielded carbon chain elongation |
EP2938732A1 (en) | 2012-12-31 | 2015-11-04 | Invista Technologies S.à.r.l. | Methods of producing 7-carbon chemicals from long chain fatty acids via oxidative cleavage |
EP2938731A2 (en) | 2012-12-31 | 2015-11-04 | Invista Technologies S.A R.L. | Methods of producing 7-carbon chemicals via carbon chain elongation associated with cyclohexane carboxylate synthesis |
EP2938736A2 (en) | 2012-12-31 | 2015-11-04 | Invista Technologies S.A R.L. | Methods of producing 7-carbon chemicals via c1 carbon chain elongation associated with coenzyme b synthesis |
WO2014105796A2 (en) | 2012-12-31 | 2014-07-03 | Invista North America S.A.R.L. | Methods of producing 7-carbon chemicals via aromatic compounds |
WO2014105794A2 (en) | 2012-12-31 | 2014-07-03 | Invista North America S.A.R.L. | Methods of producing 7-carbon chemicals via methyl-ester shielded carbon chain elongation |
WO2014105797A2 (en) | 2012-12-31 | 2014-07-03 | Invista North America S.A.R.L. | Methods of producing 7-carbon chemicals via pyruvate and succinate semialdehyde aldol condensation |
WO2015176026A1 (en) | 2014-05-15 | 2015-11-19 | Invista North America S.á.r.l. | Methods of producing 6-carbon chemicals using 2,6-diaminopimelate as precursor to 2-aminopimelate |
BR112016029388A2 (en) | 2014-06-16 | 2017-10-17 | Invista Tech Sarl | methods, reagents and cells to biosynthesize compounds |
EP3155108A1 (en) | 2014-06-16 | 2017-04-19 | Invista Technologies S.à.r.l. | Methods, reagents and cells for biosynthesizing compounds |
EP3155107A1 (en) | 2014-06-16 | 2017-04-19 | Invista Technologies S.à.r.l. | Methods, reagents and cells for biosynthesizing compounds |
US9938543B2 (en) | 2014-06-16 | 2018-04-10 | Invista North America S.A.R.L. | Methods, reagents and cells for biosynthesizing glutarate methyl ester |
CN109337938B (en) * | 2018-08-31 | 2021-05-07 | 河南师范大学 | Method for producing 2-oxoadipic acid by fermenting and degrading paraffin with alcanivorax |
Citations (5)
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WO2008000632A1 (en) * | 2006-06-29 | 2008-01-03 | Dsm Ip Assets B.V. | A method for achieving improved polypeptide expression |
WO2009113855A2 (en) * | 2008-03-11 | 2009-09-17 | Dsm Ip Assets B.V. | PREPARATION OF 6-AMINOCAPROIC ACID FROM α-KETOPIMELIC ACID |
WO2010068944A2 (en) * | 2008-12-12 | 2010-06-17 | Celexion, Llc | Biological synthesis of difunctional alkanes from carbohydrate feedstocks |
WO2010104390A2 (en) * | 2009-03-11 | 2010-09-16 | Dsm Ip Assets B.V. | Preparation of alpha-ketopimelic acid |
WO2011031147A1 (en) * | 2009-09-11 | 2011-03-17 | Dsm Ip Assets B.V. | Preparation of a compound comprising an amine group from an alpha-keto acid |
Family Cites Families (5)
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DK122686D0 (en) | 1986-03-17 | 1986-03-17 | Novo Industri As | PREPARATION OF PROTEINS |
KR100516986B1 (en) | 1997-02-19 | 2005-09-26 | 코닌클리즈케 디에스엠 엔.브이. | Process for the preparation of caprolactam in the absence of catalysts by contacting 6-aminocaproic acid derivatives with superheated steam |
DE60202478T3 (en) | 2001-07-23 | 2010-01-07 | Dsm Ip Assets B.V. | METHOD FOR PRODUCING POLYNUCLEOTIDE VARIANTS |
US7491520B2 (en) | 2004-01-19 | 2009-02-17 | Dsm Ip Assets B.V. | Biochemical synthesis of 6-amino caproic acid |
JP2007530065A (en) | 2004-04-02 | 2007-11-01 | ディーエスエム アイピー アセッツ ビー.ブイ. | Filamentous fungal mutants with improved homologous recombination efficiency |
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2011
- 2011-08-26 WO PCT/EP2011/064718 patent/WO2012031910A2/en active Application Filing
- 2011-09-09 TW TW100132607A patent/TW201217536A/en unknown
Patent Citations (5)
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WO2008000632A1 (en) * | 2006-06-29 | 2008-01-03 | Dsm Ip Assets B.V. | A method for achieving improved polypeptide expression |
WO2009113855A2 (en) * | 2008-03-11 | 2009-09-17 | Dsm Ip Assets B.V. | PREPARATION OF 6-AMINOCAPROIC ACID FROM α-KETOPIMELIC ACID |
WO2010068944A2 (en) * | 2008-12-12 | 2010-06-17 | Celexion, Llc | Biological synthesis of difunctional alkanes from carbohydrate feedstocks |
WO2010104390A2 (en) * | 2009-03-11 | 2010-09-16 | Dsm Ip Assets B.V. | Preparation of alpha-ketopimelic acid |
WO2011031147A1 (en) * | 2009-09-11 | 2011-03-17 | Dsm Ip Assets B.V. | Preparation of a compound comprising an amine group from an alpha-keto acid |
Non-Patent Citations (4)
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DATABASE EMBL [Online] 30 June 2007 (2007-06-30), "Methanococcus aeolicus Nankai-3, complete genome.", XP002639544, retrieved from EBI accession no. EMBL:CP000743 Database accession no. CP000743 * |
DREVLAND RANDY M ET AL: "Methanogen Homoaconitase Catalyzes Both Hydrolyase Reactions in Coenzyme B Biosynthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 283, no. 43, 24 October 2008 (2008-10-24), pages 28888 - 28896, XP002570860, ISSN: 0021-9258, [retrieved on 20080902], DOI: 10.1074/JBC.M802159200 * |
GRAHAM DAVID E ET AL: "Elucidation of methanogenic coenzyme biosyntheses: from spectroscopy to genomics", NATURAL PRODUCT REPORTS, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 19, no. 2, 1 April 2002 (2002-04-01), pages 133 - 147, XP002570861, ISSN: 0265-0568, DOI: 10.1039/B103714P * |
HOWELL DAVID M ET AL: "alpha-Keto acid chain elongation reactions involved in the biosynthesis of coenzyme B (7-mercaptoheptanoyl threonine phosphate) in methanogenic archaea", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 37, no. 28, 14 July 1998 (1998-07-14), pages 10108 - 10117, XP002503245, ISSN: 0006-2960, DOI: 10.1021/BI980662P * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103937841A (en) * | 2014-05-13 | 2014-07-23 | 上海交通大学 | Application of enoyl coenzyme A hydratase in adipic acid biosynthesis |
CN103937841B (en) * | 2014-05-13 | 2015-10-28 | 上海交通大学 | The application of enoyl-CoA hydratase in hexanodioic acid biosynthesizing |
Also Published As
Publication number | Publication date |
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TW201217536A (en) | 2012-05-01 |
WO2012031910A2 (en) | 2012-03-15 |
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