WO2011145908A3 - Quantitative analysis method using a nanotube having integrated enzymes - Google Patents

Quantitative analysis method using a nanotube having integrated enzymes Download PDF

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Publication number
WO2011145908A3
WO2011145908A3 PCT/KR2011/003730 KR2011003730W WO2011145908A3 WO 2011145908 A3 WO2011145908 A3 WO 2011145908A3 KR 2011003730 W KR2011003730 W KR 2011003730W WO 2011145908 A3 WO2011145908 A3 WO 2011145908A3
Authority
WO
WIPO (PCT)
Prior art keywords
bonded
nanotube
quantitative analysis
antibody
analysis
Prior art date
Application number
PCT/KR2011/003730
Other languages
French (fr)
Korean (ko)
Other versions
WO2011145908A2 (en
WO2011145908A9 (en
Inventor
김중배
오민규
이혜진
김병찬
Original Assignee
고려대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 고려대학교 산학협력단 filed Critical 고려대학교 산학협력단
Priority to KR1020117011682A priority Critical patent/KR101273453B1/en
Publication of WO2011145908A2 publication Critical patent/WO2011145908A2/en
Publication of WO2011145908A3 publication Critical patent/WO2011145908A3/en
Publication of WO2011145908A9 publication Critical patent/WO2011145908A9/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a quantitative analysis method which involves analyzing an object using an enzyme, aptamer, antibody, nanotube, and a magnetic bead or a substrate. More particularly, a binding aptamer or antibody to be bonded to an analysis object is bonded to a magnetic bead or a substrate, and the resultant structure is placed into a solution to be analyzed, bonded to the analysis object, and separated from the analysis object by magnetic force; another aptamer or antibody to be selectively bonded to the analysis object is bonded to the surface of the nanotube, on which enzymes are integrated, so as to prepare for a quantitative analysis of the analysis object; and the quantitative analysis is performed, through an enzyme reaction, on a composite body which is prepared by binding the resultant structure to the magnetic bead or substrate to which the aptamer or antibody is bonded. Thus, a low concentration analysis object can be diagnosed, and difficulties in quantitative analysis according to conventional detection methods can be overcome using the signal-amplification effects of a nanotube having integrated enzymes.
PCT/KR2011/003730 2010-05-20 2011-05-20 Quantitative analysis method using a nanotube having integrated enzymes WO2011145908A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020117011682A KR101273453B1 (en) 2010-05-20 2011-05-20 Quantitative Analysis Using Minute Tube with Accumulated Enzyme

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100047750 2010-05-20
KR10-2010-0047750 2010-05-20

Publications (3)

Publication Number Publication Date
WO2011145908A2 WO2011145908A2 (en) 2011-11-24
WO2011145908A3 true WO2011145908A3 (en) 2012-05-31
WO2011145908A9 WO2011145908A9 (en) 2012-06-21

Family

ID=44992236

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/003730 WO2011145908A2 (en) 2010-05-20 2011-05-20 Quantitative analysis method using a nanotube having integrated enzymes

Country Status (2)

Country Link
KR (1) KR101273453B1 (en)
WO (1) WO2011145908A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101495665B1 (en) * 2014-05-09 2015-02-26 한국생명공학연구원 Method for magnetic transfer multi-fluorescence immunoassay
CN104597240B (en) * 2015-02-02 2016-06-15 广西医科大学 Graphene/class peroxidase leukemic bio-sensing method of dual signal amplification detection
CN110865183B (en) * 2019-11-25 2022-11-18 润方(长春)生物科技有限公司 Method for preparing HRP (horse radish peroxidase) labeled antibody by using magnetic beads

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020042632A (en) * 1999-07-30 2002-06-05 엔. 레이 앤더슨 Microarrays and their manufacture
JP2004532968A (en) * 2000-06-14 2004-10-28 ボード・オブ・リージェンツ，ザ・ユニヴァーシティ・オヴ・テキサス・システム Systems and methods for cell subpopulation analysis
JP2005341865A (en) * 2004-06-02 2005-12-15 Kyoto Univ Nucleic acid probe, method for detecting nucleic acid, device for analyzing nucleic acid and method for controlling reaction rate
KR100941416B1 (en) * 2005-04-30 2010-02-11 삼성전자주식회사 Bio-disc, bio-driver apparatus, and assay method using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020042632A (en) * 1999-07-30 2002-06-05 엔. 레이 앤더슨 Microarrays and their manufacture
JP2004532968A (en) * 2000-06-14 2004-10-28 ボード・オブ・リージェンツ，ザ・ユニヴァーシティ・オヴ・テキサス・システム Systems and methods for cell subpopulation analysis
JP2005341865A (en) * 2004-06-02 2005-12-15 Kyoto Univ Nucleic acid probe, method for detecting nucleic acid, device for analyzing nucleic acid and method for controlling reaction rate
KR100941416B1 (en) * 2005-04-30 2010-02-11 삼성전자주식회사 Bio-disc, bio-driver apparatus, and assay method using the same

Also Published As

Publication number Publication date
WO2011145908A2 (en) 2011-11-24
KR20110130384A (en) 2011-12-05
WO2011145908A9 (en) 2012-06-21
KR101273453B1 (en) 2013-06-11

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