WO2011139132A3 - 줄기세포로부터 생성된 배아체를 대량 증식 및 유지하는 방법 - Google Patents

줄기세포로부터 생성된 배아체를 대량 증식 및 유지하는 방법 Download PDF

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WO2011139132A3
WO2011139132A3 PCT/KR2011/003439 KR2011003439W WO2011139132A3 WO 2011139132 A3 WO2011139132 A3 WO 2011139132A3 KR 2011003439 W KR2011003439 W KR 2011003439W WO 2011139132 A3 WO2011139132 A3 WO 2011139132A3
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embryoid bodies
stem cells
maintaining
produced
embryoid
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PCT/KR2011/003439
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English (en)
French (fr)
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WO2011139132A2 (ko
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조이숙
손미영
김현진
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한국생명공학연구원
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Publication of WO2011139132A2 publication Critical patent/WO2011139132A2/ko
Publication of WO2011139132A3 publication Critical patent/WO2011139132A3/ko
Priority to US13/669,896 priority Critical patent/US8986996B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/09—Means for pre-treatment of biological substances by enzymatic treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Mechanical Engineering (AREA)
  • Clinical Laboratory Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

본 발명은 줄기세포로부터 생성된 배아체를 대량 증식 및 유지하는 방법에 관한 것으로, 구체적으로 배아줄기세포 또는 유도 만능줄기세포로부터 일차적으로 제작된 배아체를 연속적으로 계대 배양하는 단계를 포함하는, 배아체를 장시간 배양하면서 본래의 성질을 유지한 채 대량 증식 및 유지하는 방법에 관한 것이다. 본 발명의 방법을 이용하면 제한된 양의 줄기세포 분획으로부터 배아체를 제작한 후, 지속적으로 줄기세포를 소모하지 않고도 기술적으로 단순한 기계적 계대 배양 방법을 이용하여 배아체를 대량 생산 및 유지하는 것이 가능하다.
PCT/KR2011/003439 2010-05-07 2011-05-09 줄기세포로부터 생성된 배아체를 대량 증식 및 유지하는 방법 WO2011139132A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/669,896 US8986996B2 (en) 2010-05-07 2012-11-06 Large-scale propagation and maintenance method of embryoid bodies generated from stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100043211 2010-05-07
KR10-2010-0043211 2010-05-07

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/669,896 Continuation-In-Part US8986996B2 (en) 2010-05-07 2012-11-06 Large-scale propagation and maintenance method of embryoid bodies generated from stem cells

Publications (2)

Publication Number Publication Date
WO2011139132A2 WO2011139132A2 (ko) 2011-11-10
WO2011139132A3 true WO2011139132A3 (ko) 2012-05-03

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PCT/KR2011/003439 WO2011139132A2 (ko) 2010-05-07 2011-05-09 줄기세포로부터 생성된 배아체를 대량 증식 및 유지하는 방법

Country Status (3)

Country Link
US (1) US8986996B2 (ko)
KR (1) KR101330327B1 (ko)
WO (1) WO2011139132A2 (ko)

Families Citing this family (5)

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CN106434527A (zh) 2006-04-14 2017-02-22 安斯泰来再生医药协会 血管瘤集落形成细胞
EP2949746A4 (en) * 2013-01-23 2016-07-27 Tokyo Electron Ltd METHOD OF SUB-CULTURE OF PLURIPOTENT STEM CELLS
KR101919953B1 (ko) * 2014-10-20 2018-11-20 중앙대학교 산학협력단 트립신 프리 세포 스탬프 시스템 및 이의 용도
WO2017083705A1 (en) * 2015-11-13 2017-05-18 The Johns Hopkins University Cell culture system and method of use thereof
WO2018232079A1 (en) 2017-06-14 2018-12-20 Daley George Q Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer

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ATE512218T1 (de) * 2002-01-09 2011-06-15 Barrueta Alberto Gorrochategui Zusammensetzung zur herstellung, regeneration und reparatur von geweben unter verwendung eines zellentragenden biologischen implantats, das mit einem blutplättchenkonzentrat und supplementen angereichert ist
WO2005027623A1 (ja) * 2003-09-16 2005-03-31 Keiichi Shimizu 変化アサガオの効率的な増殖法
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EP2088190A4 (en) 2006-11-09 2011-01-05 Japan Government METHOD FOR THE CULTURE AND PASSAGE OF PRIMATE EMBRYONIC STRAIN CELL, AND METHOD FOR INDUCING DIFFERENTIATION OF EMBRYONIC STEM CELL

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KHOO, M.L.M. ET AL.: "Growth and Differentiation of Embryoid Bodies Derived from Human Embryonic Stem Cells: Effect of Glucose and Basic Fibroblast Growth Factor", BIOLOGY OF REPRODUCTION, vol. 73, 3 August 2005 (2005-08-03), pages 1147 - 1156, XP008131015, DOI: doi:10.1095/biolreprod.104.036673 *
RUNGARUNLERT, S. ET AL.: "Embryoid Body Formation from Embryonic and Induced Pluripotent Stem Cells: Benefits of Bioreactors", WORLD JOURNAL OF STEM CELLS, vol. 1, no. 1, 31 December 2009 (2009-12-31), pages 11 - 21, XP055243678, DOI: doi:10.4252/wjsc.v1.i1.11 *
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Also Published As

Publication number Publication date
US20130224856A1 (en) 2013-08-29
US8986996B2 (en) 2015-03-24
WO2011139132A2 (ko) 2011-11-10
KR101330327B1 (ko) 2013-11-14
KR20110123702A (ko) 2011-11-15

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