WO2011136527A3 - 나노유체역학 기반의 형광 근접장 현미경 - Google Patents

나노유체역학 기반의 형광 근접장 현미경 Download PDF

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Publication number
WO2011136527A3
WO2011136527A3 PCT/KR2011/003008 KR2011003008W WO2011136527A3 WO 2011136527 A3 WO2011136527 A3 WO 2011136527A3 KR 2011003008 W KR2011003008 W KR 2011003008W WO 2011136527 A3 WO2011136527 A3 WO 2011136527A3
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WIPO (PCT)
Prior art keywords
narrow space
fluorescence
nanoantenna
miscroscope
scanning optical
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PCT/KR2011/003008
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English (en)
French (fr)
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WO2011136527A2 (ko
WO2011136527A9 (ko
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천홍구
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서울대학교산학협력단
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Application filed by 서울대학교산학협력단 filed Critical 서울대학교산학협력단
Publication of WO2011136527A2 publication Critical patent/WO2011136527A2/ko
Publication of WO2011136527A9 publication Critical patent/WO2011136527A9/ko
Publication of WO2011136527A3 publication Critical patent/WO2011136527A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
    • G01Q60/18—SNOM [Scanning Near-Field Optical Microscopy] or apparatus therefor, e.g. SNOM probes
    • G01Q60/20—Fluorescence
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
    • B82B1/00—Nanostructures formed by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)

Abstract

나노유체역학 기반의 형광 근접장 현미경이 제공된다. 본 형광 근접장 현미경은, 입사광원을 좁은공간에 집중시키고 상기 좁은공간 안에 존재하는 형광시료의 양자수율(quantum yield)을 변경시키며 상기 좁은공간 안에서 발생한 형광신호출력을 특정방향으로 집중시키도록 구성된 나노안테나, 상기 나노안테나의 상기 좁은공간에 연결되어, 상기 좁은공간으로 형광시료를 도입하기 위한 이동통로를 제공하는 나노포어 또는 나노채널을 포함한다. 이에 의해, 나노안테나의 특정 좁은공간에 입사광원을 집중시키고 형광시료의 낮은 양자수율을 높이며 형광신호출력을 특정방향에서 효율적으로 검출할 수 있게되어, 높은 신호대잡음비 및 고분해능 형광검출이 가능해지고, 나노포어 또는 나노채널을 통해 형광시료를 나노안테나로 도입시킴으로써, 나노안테나의 기계적 움직임 없이 시료의 스캐닝이 가능해진다. 상기 나노유체역학 기반의 형광 근접장 현미경은, 형광표지된 DNA를 상기 나노포어 또는 나노채널을 통해 선형화하여 통과 시키면서, 상기 좁은공간 안에서 순차적으로 발생한 형광신호를 읽음으로써 DNA 염기서열 분석이 가능하다.
PCT/KR2011/003008 2010-04-29 2011-04-26 나노유체역학 기반의 형광 근접장 현미경 WO2011136527A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0039815 2010-04-29
KR1020100039815A KR101681951B1 (ko) 2010-04-29 2010-04-29 나노유체역학 기반의 형광 근접장 현미경

Publications (3)

Publication Number Publication Date
WO2011136527A2 WO2011136527A2 (ko) 2011-11-03
WO2011136527A9 WO2011136527A9 (ko) 2011-12-29
WO2011136527A3 true WO2011136527A3 (ko) 2012-04-19

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PCT/KR2011/003008 WO2011136527A2 (ko) 2010-04-29 2011-04-26 나노유체역학 기반의 형광 근접장 현미경

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KR (1) KR101681951B1 (ko)
WO (1) WO2011136527A2 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013112698A1 (en) 2012-01-24 2013-08-01 Src, Inc. Methods and systems for long distance tagging, tracking, and locating using wavelength upconversion
US9718668B2 (en) 2012-02-16 2017-08-01 Board Of Trustees Of The University Of Arkansas Method of fabricating a nanochannel system for DNA sequencing and nanoparticle characterization
CN109261230B (zh) * 2018-09-30 2020-05-08 东南大学 一种光控纳米孔的单分子可控输出装置及其使用方法
CN114113017B (zh) * 2021-11-29 2024-02-23 中国科学院重庆绿色智能技术研究院 一种基于固态纳米孔的功能蛋白光电联合检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003016781A2 (en) * 2001-08-14 2003-02-27 The President And Fellows Of Harvard College Surface plasmon enhanced illumination system
US20040190116A1 (en) * 2001-08-31 2004-09-30 Lezec Henri Joseph Optical transmission apparatus with directionality and divergence control
WO2008036697A2 (en) * 2006-09-18 2008-03-27 Applera Corporation Methods, systems and apparatus for light concentrating mechanisms
EP2133688A1 (en) * 2008-06-11 2009-12-16 Koninklijke Philips Electronics N.V. Nanoantenna and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007025013A2 (en) * 2005-08-24 2007-03-01 The Trustees Of Boston College Nanoscale optical microscope

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003016781A2 (en) * 2001-08-14 2003-02-27 The President And Fellows Of Harvard College Surface plasmon enhanced illumination system
US20040190116A1 (en) * 2001-08-31 2004-09-30 Lezec Henri Joseph Optical transmission apparatus with directionality and divergence control
WO2008036697A2 (en) * 2006-09-18 2008-03-27 Applera Corporation Methods, systems and apparatus for light concentrating mechanisms
EP2133688A1 (en) * 2008-06-11 2009-12-16 Koninklijke Philips Electronics N.V. Nanoantenna and uses thereof

Also Published As

Publication number Publication date
KR20110120415A (ko) 2011-11-04
WO2011136527A2 (ko) 2011-11-03
KR101681951B1 (ko) 2016-12-05
WO2011136527A9 (ko) 2011-12-29

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