WO2011132939A3 - Rtk-bpb specifically binding to rtk - Google Patents

Rtk-bpb specifically binding to rtk Download PDF

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Publication number
WO2011132939A3
WO2011132939A3 PCT/KR2011/002839 KR2011002839W WO2011132939A3 WO 2011132939 A3 WO2011132939 A3 WO 2011132939A3 KR 2011002839 W KR2011002839 W KR 2011002839W WO 2011132939 A3 WO2011132939 A3 WO 2011132939A3
Authority
WO
WIPO (PCT)
Prior art keywords
rtk
present
peptide binder
bipodal peptide
specifically binding
Prior art date
Application number
PCT/KR2011/002839
Other languages
French (fr)
Korean (ko)
Other versions
WO2011132939A2 (en
WO2011132939A8 (en
Inventor
전상용
김성현
박세호
김대진
이상헌
Original Assignee
광주과학기술원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 광주과학기술원 filed Critical 광주과학기술원
Priority to KR1020127023553A priority Critical patent/KR20130103302A/en
Publication of WO2011132939A2 publication Critical patent/WO2011132939A2/en
Publication of WO2011132939A3 publication Critical patent/WO2011132939A3/en
Publication of WO2011132939A8 publication Critical patent/WO2011132939A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to an RTK-bipodal peptide binder specifically binding to RTK, comprising: (a) a structure stabilizing region comprising parallel, antiparallel, or parallel and antiparallel amino acid strands in which interstrand non-covalent bonds are formed; and (b) an RTK-target binding region I and an RTK-target binding region II which bond to both terminals of the structure stabilizing region and which comprise n and m amino acids randomly selected, respectively. The RTK-bipodal peptide binder of the present invention exhibits a very low KD value (dissociation constant) (for example, nM level) relative to RTK and therefore exhibits a very high affinity toward an RTK target. The RTK-bipodal peptide binder of the present invention may have a use as a medicine, may be used for in vivo molecular imaging and drug delivery targeting, and may be very useful as an escort molecule.
PCT/KR2011/002839 2010-04-20 2011-04-20 Rtk-bpb specifically binding to rtk WO2011132939A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020127023553A KR20130103302A (en) 2010-04-20 2011-04-20 Cytokine-bpb specifically binding to cytokine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100036607 2010-04-20
KR10-2010-0036607 2010-04-20

Publications (3)

Publication Number Publication Date
WO2011132939A2 WO2011132939A2 (en) 2011-10-27
WO2011132939A3 true WO2011132939A3 (en) 2012-04-05
WO2011132939A8 WO2011132939A8 (en) 2012-08-09

Family

ID=44834645

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/002839 WO2011132939A2 (en) 2010-04-20 2011-04-20 Rtk-bpb specifically binding to rtk

Country Status (2)

Country Link
KR (1) KR20130103302A (en)
WO (1) WO2011132939A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
JP2001002592A (en) * 1999-06-18 2001-01-09 Dai Ichi Seiyaku Co Ltd Composition for gene-transducing
US20040152647A1 (en) * 2003-01-31 2004-08-05 Max-Delbruck-Cenrum Fur Molekulare Medizin Agent for gene transfer
US6914123B2 (en) * 2001-04-17 2005-07-05 Genentech, Inc. Hairpin peptides with a novel structural motif and methods relating thereto
WO2010047515A2 (en) * 2008-10-20 2010-04-29 광주과학기술원 Bipodal peptide binder

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
JP2001002592A (en) * 1999-06-18 2001-01-09 Dai Ichi Seiyaku Co Ltd Composition for gene-transducing
US6914123B2 (en) * 2001-04-17 2005-07-05 Genentech, Inc. Hairpin peptides with a novel structural motif and methods relating thereto
US20040152647A1 (en) * 2003-01-31 2004-08-05 Max-Delbruck-Cenrum Fur Molekulare Medizin Agent for gene transfer
WO2010047515A2 (en) * 2008-10-20 2010-04-29 광주과학기술원 Bipodal peptide binder

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TIM CLACKSON ET AL.: "Making antibody fragments using phage display libraries", NATURE, vol. 352, 15 August 1991 (1991-08-15), pages 624 - 628 *
VAISHALI BAGALKOT ET AL.: "An Aptamer-Doxorubicin Physical Conjugate as a Novel Targeted Drug-Delivery Platform", ANGEW. CHEM. INT. ED., vol. 45, 2006, pages 8149 - 8152 *

Also Published As

Publication number Publication date
WO2011132939A2 (en) 2011-10-27
KR20130103302A (en) 2013-09-23
WO2011132939A8 (en) 2012-08-09

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