WO2011109031A8 - Methods for cloning and manipulating genomes - Google Patents

Methods for cloning and manipulating genomes Download PDF

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Publication number
WO2011109031A8
WO2011109031A8 PCT/US2010/035490 US2010035490W WO2011109031A8 WO 2011109031 A8 WO2011109031 A8 WO 2011109031A8 US 2010035490 W US2010035490 W US 2010035490W WO 2011109031 A8 WO2011109031 A8 WO 2011109031A8
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cloning
genomes
donor
host cell
Prior art date
Application number
PCT/US2010/035490
Other languages
French (fr)
Other versions
WO2011109031A1 (en
Inventor
Gwynedd A. Benders
John I. Glass
Clyde A. Hutchison
Carole Lartigue
Sanjay Vashee
Mikkel A. Algire
Hamilton O. Smith
Charles E. Merryman
Vladimir N. Noskov
Ray-Yuan Chuang
Daniel G. Gibson
J. Craig Venter
Original Assignee
Synthetic Genomics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/718,911 external-priority patent/US9273310B2/en
Application filed by Synthetic Genomics, Inc. filed Critical Synthetic Genomics, Inc.
Priority to JP2012556048A priority Critical patent/JP2013520989A/en
Priority to CN2010800666205A priority patent/CN102939380A/en
Priority to EP10726723A priority patent/EP2542676A1/en
Publication of WO2011109031A1 publication Critical patent/WO2011109031A1/en
Publication of WO2011109031A8 publication Critical patent/WO2011109031A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Abstract

Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
PCT/US2010/035490 2010-03-05 2010-05-19 Methods for cloning and manipulating genomes WO2011109031A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2012556048A JP2013520989A (en) 2010-03-05 2010-05-19 Methods for genome cloning and manipulation
CN2010800666205A CN102939380A (en) 2010-03-05 2010-05-19 Methods for cloning and manipulating genomes
EP10726723A EP2542676A1 (en) 2010-03-05 2010-05-19 Methods for cloning and manipulating genomes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12/718,911 2010-03-05
US12/718,911 US9273310B2 (en) 2007-10-08 2010-03-05 Methods for cloning and manipulating genomes
US32226910P 2010-04-08 2010-04-08
US61/322,269 2010-04-08

Publications (2)

Publication Number Publication Date
WO2011109031A1 WO2011109031A1 (en) 2011-09-09
WO2011109031A8 true WO2011109031A8 (en) 2012-09-20

Family

ID=42697547

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/035490 WO2011109031A1 (en) 2010-03-05 2010-05-19 Methods for cloning and manipulating genomes

Country Status (4)

Country Link
EP (1) EP2542676A1 (en)
JP (1) JP2013520989A (en)
CN (1) CN102939380A (en)
WO (1) WO2011109031A1 (en)

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Also Published As

Publication number Publication date
CN102939380A (en) 2013-02-20
WO2011109031A1 (en) 2011-09-09
JP2013520989A (en) 2013-06-10
EP2542676A1 (en) 2013-01-09

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