WO2011088031A1 - Composés et procédés - Google Patents

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WO2011088031A1
WO2011088031A1 PCT/US2011/020809 US2011020809W WO2011088031A1 WO 2011088031 A1 WO2011088031 A1 WO 2011088031A1 US 2011020809 W US2011020809 W US 2011020809W WO 2011088031 A1 WO2011088031 A1 WO 2011088031A1
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alkyl
amino
crc
haloalkyl
alkoxy
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PCT/US2011/020809
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English (en)
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Lara S. Kallander
Joanne Philp
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Glaxosmithkline Llc
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Publication of WO2011088031A1 publication Critical patent/WO2011088031A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
    • C07D239/48—Two nitrogen atoms

Definitions

  • the present invention relates to compounds that inhibit TNNI3K and methods of making and using the same. Specifically, the present invention relates to
  • Cardiac troponin l-interacting kinase (TNNI3K), also known as CARK (for cardiac ankyrin repeat kinase), is a protein kinase that exhibits highly selective expression for cardiac tissues and has been shown to interact with components of the sarcomere, including troponin I (Zhao, Y. et al., J. Mol. Med., 2003, 81, 297-304; Feng, Y. et al., Gen. Physiol. Biophys., 2007, 26, 104-109; Wang, H. et al., J. Cell. Mol. Med., 2008, 12, 304- 315).
  • TNNI3K a cardiac-specific kinase, promotes cardiac hypertrophy in vivo
  • Inhibition of the kinase activity of TNNI3K may disrupt these signaling pathways, and enable the mitigation and/or reversal of cardiac hypertrophy seen in patients with progressively worsening heart failure.
  • the heart In response to mechanical, neurohormonal, and genetic stimuli, the heart will undergo hypertrophy, or muscle growth and remodeling, in order to maintain sufficient cardiac output to meet tissue oxygen demands. While these structural changes are initially seen as compensatory, sustained dysregulation of hypertrophic signaling can lead to heart failure, the pathophysiological state in which the heart can no longer adequately function as a pump (Mudd, J. O. and Kass, D. A., Nature, 2008, 451, 919-928).
  • Heart failure is responsible for a reduced quality of life and premature death in a significant proportion of sufferers, and is characterized by impaired cardiac function either due to reduced pump function (systolic dysfunction) or reduced filling (diastolic dysfunction).
  • Congestive heart failure is characterized by impaired left ventricular function, increased peripheral and pulmonary vascular resistance and reduced exercise tolerance and dyspnea. The prevalence of heart failure is anticipated to increase with ageing populations, prompting a need for new and improved methods of treating heart failure.
  • the invention is directed to novel diaminotriazines. Specifically, the invention is directed to compounds according to Formula I:
  • R 1 is (CrC 4 )alkyl
  • R 2 is hydrogen or halogen
  • R 3 is hydrogen, halogen, (d-C 4 )alkyl, (Ci-C 4 )haloalkyl, (C 3 -C 6 )cycloalkyl, aryl, hydroxyl, hydroxy(Ci-C 4 )alkyl-, (Ci-C 4 )alkoxy, (Ci-C 4 )alkoxy(Ci-C 4 )alkyl-,
  • R 4 is hydrogen, halogen, (CrC 8 )alkyl, (Ci-C 8 )haloalkyl, (C 3 -C 8 )cycloalkyl, hydroxyl, hydroxy(CrC 8 )alkyl-, (Ci-C 8 )alkoxy, (Ci-C 4 )alkoxy(CrC 8 )alkyl-, (Ci-C 8 )haloalkoxy, (C 3 -C 8 )cycloalkyloxy, (Ci-C 8 )alkylthio-, (Ci-C 8 )haloalkylthio-, -S0 2 (CrC 4 )alkyl, amino, -NHR 7 , or -NR 7 R 8 ;
  • R 5 is hydrogen
  • R 4 and R 5 taken together with atoms through which they are connected form a 5 or 6 membered ring, optionally containing one or two additional heteroatoms selected from N, O and S, which ring may be unsubstituted or substituted with one to three substituents independently selected from (d-C 4 )alkyl, (Ci-C 4 )haloalkyl, hydroxy(CrC 4 )alkyl-, oxo, hydroxyl, (Ci-C 4 )alkoxy, (Ci-C 4 )haloalkoxy, and (Ci-C 4 )alkylthio-;
  • R 6 is (CrCe)alkyl, (C 2 -C 8 )alkenyl, (C 2 -C 8 )alkynyl, (C 3 -C 8 )cycloalkyl, aryl, or heteroaryl, wherein any aryl or heteroaryl group is optionally substituted one to three times, independently, by halogen, (d-C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl,
  • R 7 is (CrC 4 )alkyl, aryl, heterocycloalkyl, or heterocycloalkyl(Ci-C 2 )alkyl, wherein said (CrC 4 )alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (Ci-C 4 )alkoxy, amino, (Ci-C 4 )alkylamino, ((Ci-C 4 )alkyl)((Ci-C 4 )alkyl)amino, -C0 2 H, -C0 2 (CrC 4 )alkyl, -CONH 2 , -CONH(C C 4 )alkyl, or
  • R 8 is (Ci-C 4 )alkyl
  • R 7 and R 8 taken together with the nitrogen to which they are attached represent a 5-7 membered heterocyclic ring, optionally containing an additional heteroatom selected from oxygen, nitrogen, and sulfur, wherein said ring is optionally substituted one or two times, independently, by halogen, (Ci-C 4 )alkyl, (Ci-C 4 )haloalkyl, amino,
  • the compounds of the invention are inhibitors of TNNI3K and can be useful for the treatment of cardiac diseases and disorders, particularly heart failure. Accordingly, the invention is further directed to pharmaceutical compositions comprising a compound of the invention. The invention is still further directed to methods of inhibiting TNNI3K and treatment of conditions associated therewith using a compound of the invention or a pharmaceutical composition comprising a compound of the invention.
  • alkyl represents a saturated, straight or branched hydrocarbon moiety, which may be unsubstituted or substituted by one or more of the substituents defined herein.
  • exemplary alkyls include, but are not limited to methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, s-butyl, f-butyl, pentyl, and hexyl.
  • C1-C4" refers to an alkyl containing from 1 to 4 carbon atoms.
  • alkyl When the term “alkyl” is used in combination with other substituent groups, such as “haloalkyl”, “hydroxyalkyl”, or “alkoxyalkyl”, the term “alkyl” is intended to encompass a divalent straight or branched-chain hydrocarbon radical.
  • alkenyl refers to straight or branched hydrocarbon chains containing the specified number of carbon atoms and at least 1 and up to 3 carbon-carbon double bonds. Examples include ethenyl and propenyl.
  • alkynyl refers to straight or branched hydrocarbon chains containing the specified number of carbon atoms and at least 1 and up to 3 carbon-carbon triple bonds. Examples include ethynyl and propynyl.
  • cycloalkyl refers to a non-aromatic, saturated, cyclic hydrocarbon ring.
  • (C 3 -C 8 )cycloalkyl refers to a non-aromatic cyclic
  • hydrocarbon ring having from three to eight ring carbon atoms.
  • (C 3 -C 8 )cycloalkyl groups useful in the present invention include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.
  • Alkoxy refers to a group containing an alkyl radical attached through an oxygen linking atom.
  • (CrC 4 )alkoxy refers to a straight- or branched-chain
  • hydrocarbon radical having at least 1 and up to 4 carbon atoms attached through an oxygen linking atom.
  • exemplary "(d-C 4 )alkoxy" groups useful in the present invention include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, s-butoxy, and f-butoxy.
  • Alkylthio- refers to a group containing an alkyl radical attached through a sulfur linking atom.
  • the term "(C 1 -C 4 )alkylthio-” refers to a straight- or branched-chain hydrocarbon radical having at least 1 and up to 4 carbon atoms attached through a sulfur linking atom.
  • Exemplary "(C 1 -C 4 )alkylthio-” groups useful in the present invention include, but are not limited to, methylthio-, ethylthio-, n-propylthio-, isopropylthio-, n-butylthio-, s-butylthio-, and f-butylthio-.
  • Cycloalkyloxy refers to a group containing a saturated carbocyclic ring attached through an oxygen linking atom.
  • Examples of “cycloalkyloxy” moieties include, but are not limited to, cyclopropyloxy, cyclobutyloxy, cyclopentyloxy, cyclohexyloxy, and the like.
  • Aryl represents a group or moiety comprising an aromatic, monovalent monocyclic or bicyclic hydrocarbon radical containing from 6 to 10 carbon ring atoms, which may be unsubstituted or substituted by one or more of the substituents defined herein, and to which may be fused to one or more cycloalkyl rings, which may be unsubstituted or substituted by one or more substituents defined herein.
  • aryl is phenyl
  • Heterocyclic groups may be heteroaryl or heterocycloalkyl groups.
  • Heterocycloalkyl represents a group or moiety comprising a non-aromatic, monovalent monocyclic or bicyclic radical, which is saturated or partially unsaturated, containing 3 to 10 ring atoms, which includes 1 to 3 heteroatoms selected from nitrogen, oxygen and sulfur, and which may be unsubstituted or substituted by one or more of the substituents defined herein.
  • heterocycloalkyls include, but are not limited to, azetidinyl, pyrrolidinyl, pyrazolidinyl, pyrazolinyl, imidazolidinyl, imidazolinyl, oxazolinyl, thiazolinyl, tetrahydrofuranyl, dihydrofuranyl, 1 ,3-dioxolanyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, tetrahydropyranyl, dihydropyranyl, 1 ,3-dioxanyl, 1 ,4-dioxanyl, 1 ,3-oxathiolanyl, 1 ,3-oxathianyl, 1 ,3-dithianyl, hexahydro-1 H-1 ,4-diazepinyl, azabicylo[3.2.1 ]o
  • heterocycloalkyl groups are 5-7 membered heterocycloalkyl groups, such as pyrrolidinyl, pyrazolidinyl, pyrazolinyl, imidazolidinyl, imidazolinyl, oxazolinyl, thiazolinyl, tetrahydrofuranyl, dihydrofuranyl, 1 ,3- dioxolanyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, tetrahydropyranyl, dihydropyranyl, and hexahydro-1 H-1 ,4-diazepinyl.
  • heterocycloalkyl groups are 5-7 membered heterocycloalkyl groups, such as pyrrolidinyl, pyrazolidinyl, pyrazolinyl, imidazolidinyl, imidazolinyl, oxazolinyl, thiazolin
  • Heteroaryl represents a group or moiety comprising an aromatic monovalent monocyclic or bicyclic radical, containing 5 to 10 ring atoms, including 1 to 4 heteroatoms selected from nitrogen, oxygen and sulfur, which may be unsubstituted or substituted by one or more of the substituents defined herein.
  • This term also encompasses bicyclic heterocyclic-aryl compounds containing an aryl ring moiety fused to a heterocycloalkyl ring moiety, containing 5 to 10 ring atoms, including 1 to 4 heteroatoms selected from nitrogen, oxygen and sulfur, which may be unsubstituted or substituted by one or more of the substituents defined herein.
  • heteroaryls include, but are not limited to, furanyl, thienyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, thiazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiadiazolyl, isothiazolyl, pyridinyl, pyridazinyl, pyrazinyl, pyrimidinyl, triazinyl, benzofuranyl, isobenzofuryl, 2,3-dihydrobenzofuryl,
  • heteroaryl groups present in the compounds of this invention are 5-membered and/or 6-memebred monocyclic heteroaryl groups.
  • Selected 5-membered heteroaryl groups contain one nitrogen, oxygen or sulfur ring heteroatom, and optionally contain 1 , 2, or 3 additional nitrogen ring atoms.
  • Selected 6-membered heteroaryl groups contain 1 , 2, or 3 nitrogen ring heteroatoms.
  • Selected 5- or 6-membered heteroaryl groups include furanyl, thienyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, thiazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiadiazolyl, isothiazolyl, pyridinyl, pyridazinyl, pyrazinyl, pyrimidinyl, and triazinyl.
  • halogen and halo represent chloro, fluoro, bromo or iodo substituents.
  • the term "compound(s) of the invention” means a compound of Formula I (as defined above) in any form, i.e., any salt or non-salt form (e.g., as a free acid or base form, or as a pharmaceutically acceptable salt thereof) and any physical form thereof (e.g., including non-solid forms (e.g., liquid or semi-solid forms), and solid forms (e.g., amorphous or crystalline forms, specific polymorphic forms, solvates, including hydrates (e.g., mono-, di- and hemi- hydrates)), and mixtures of various forms.
  • any salt or non-salt form e.g., as a free acid or base form, or as a pharmaceutically acceptable salt thereof
  • any physical form thereof e.g., including non-solid forms (e.g., liquid or semi-solid forms), and solid forms (e.g., amorphous or crystalline forms, specific polymorphic forms, solvates, including hydrates (
  • R 1 is (Ci-C 4 )alkyl. In a specific embodiment of this invention, R 1 is methyl.
  • R 2 is hydrogen or halogen.
  • R 2 is hydrogen or fluorine.
  • R 2 is hydrogen.
  • R 3 is hydrogen, halogen, (d-C 4 )alkyl, (CrC 4 )haloalkyl, (C 3 -C 6 )cycloalkyl, aryl, hydroxyl, hydroxy(CrC 4 )alkyl-, (CrC 4 )alkoxy, (Ci-C 4 )alkoxy(CrC 4 )alkyl-,
  • R 3 is hydrogen, halogen, (CrC 4 )alkyl, (CrC 4 )haloalkyl, phenyl, (Ci-C 4 )alkoxy, (CrC 4 )alkylthio-, or ((Ci-C 4 )alkyl)((Ci-C 4 )alkyl)amino.
  • R 3 is hydrogen, chlorine, or dimethylamino.
  • R 3 is hydrogen.
  • R 2 and R 3 are each hydrogen.
  • R 4 is hydrogen, halogen, (C 1 -C 8 )alkyl, (C 1 -C 8 )haloalkyl, (C 3 -C 8 )cycloalkyl, hydroxyl, hydroxy(C 1 -C 8 )alkyl-, (C 1 -C 8 )alkoxy, (C 1 -C 4 )alkoxy(C 1 -C 8 )alkyl-,
  • R 4 is hydrogen, halogen, (Ci-C 8 )alkyl, (Ci-C 8 )haloalkyl, (C 3 -C 8 )cycloalkyl, hydroxyl, hydroxy(Ci-C 8 )alkyl-, (C C 8 )alkoxy, (Ci-C 4 )alkoxy(CrC 8 )alkyl-, (C C 8 )haloalkoxy, (C 3 -C 8 )cycloalkyloxy, (Ci-C 8 )alkylthio-, (Ci-C 8 )haloalkylthio-, -S0 2 (Ci-C 4 )alkyl, amino, (Ci-C 4 )alkylamino, (Ci-C 4 )haloalkylamino, (
  • R 4 is hydrogen, halogen, (Ci-C 8 )alkyl, (Ci-C 8 )haloalkyl, (C 3 -C 8 )cycloalkyl, hydroxyl, hydroxy(Ci-C 8 )alkyl-, (Ci-C 8 )alkoxy, (Ci-C 4 )alkoxy(CrC 8 )alkyl-, (Ci-C 8 )haloalkoxy, (C 3 -C 8 )cycloalkyloxy, (Ci-C 8 )alkylthio-, -S0 2 (Ci-C 4 )alkyl, amino, (Ci-C 4 )alkylamino,
  • R 4 is hydrogen, fluorine, chlorine, hydroxyl, methoxy, ethoxy, n-propyloxy, isopropyloxy, isobutyloxy, 3-methyl-2-butyloxy, 3-pentyloxy, trifluoromethoxy, 2,2,2-trifluoroethoxy, 1 , 1 , 1-trifluoro-2-propyloxy, 3,3,3-trifluoro-1-propyloxy, 1 , 1 ,1 -trifluoro-2-methyl-2-propyloxy, 1 , 1 ,1 ,3,3,3-hexafluoro-2-methyl-2-propyloxy, cyclopentyloxy, cyclohexyloxy, methylthio-, ethylthio-, isobutylthio-, 2,2,2-trifluoroethylthio-, methylsulfone, ethylsulfone,
  • R 4 is hydrogen, dimethylamino, or morpholin-4-yl. In a particular embodiment of this invention, R 4 is hydrogen.
  • R 4 and R 5 taken together with atoms through which they are connected form a 5 or 6 membered ring, optionally containing one or two additional heteroatoms selected from N, O and S, which ring may be unsubstituted or substituted with one to three substituents independently selected from (d-C 4 )alkyl, (Ci-C 4 )haloalkyl, hydroxy(Ci-C 4 )alkyl-, oxo, hydroxyl, (Ci-C 4 )alkoxy, (Ci-C 4 )haloalkoxy, and (C 1 -C 4 )alkylthio-.
  • R 4 and R 5 taken together with atoms through which they are connected form a partially saturated 5 or 6 membered ring, optionally containing one or two additional heteroatoms selected from N, O and S, which ring may be unsubstituted or substituted with one to three substituents independently selected from (Ci-C 4 )alkyl, (Ci-C 4 )haloalkyl, hydroxy(Ci-C 4 )alkyl-,
  • R 4 and R 5 taken together represent -CH 2 CH 2 -.
  • R 6 is (Ci-C 8 )alkyl, (C 2 -C 8 )alkenyl, (C 2 -C 8 )alkynyl, (C 3 -C 8 )cycloalkyl, aryl, or heteroaryl, wherein any aryl or heteroaryl group is optionally substituted one to three times, independently, by halogen, (CrC 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl,
  • R 6 is (C 1 -C 6 )alkyl, phenyl, dihydroindenyl, tetrahydronaphthalenyl, oxazolyl, thiazolyl, thiadiazolyl, pyridinyl, pyrimidinyl, indolyl, indazolyl, dihydroindolyl, dihydroisoindolyl, chromenyl, dihydrobenzimidazolyl,
  • R 6 is (CrC 6 )alkyl, phenyl, oxazolyl, thiazolyl, thiadiazolyl, pyridinyl, indolyl, indazolyl, dihydroindolyl, dihydrobenzimidazolyl, dihydrobenzoxazolyl, benzthiazolyl, dihydrobenzoisothiazolyl, quinolinyl, isoquinolinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, or dihydrobenzodioxinyl, wherein said phenyl, oxazolyl, thiazolyl, thiadiazolyl, pyridinyl, indolyl, indazolyl, dihydroindolyl, dihydrobenzimidazolyl, dihydrobenzoxazolyl, benzthiazolyl, dihydrobenzo
  • dihydrobenzodioxinyl group is optionally substituted one or two times, independently, by halogen, (C C 6 )alkyl, (C 3 -C 6 )cycloalkyl, (C C 4 )haloalkyl, cyano, -CO(C C 4 )alkyl, -C0 2 H, -C0 2 R 7 , -CONH 2 , -CONHR 7 , -CONR 7 R 8 , H0 2 C(C C 2 )alkyl-, R 7 0 2 C(Ci-C 2 )alkyl-, -SR 7 , -S0 2 (d-C 4 )alkyl, -S0 2 NH 2 , -S0 2 NHR 7 , -S0 2 NR 7 R 8 , nitro, amino, -NHR 7 , -NR 7 R 8 , amino(C C 2 )alkyl-, R 7 HN(C C 2 )alky
  • -NHS0 2 (d-C 4 )alkyl oxo, hydroxyl, -OR 7 , hydroxy(C C 2 )alkyl-, R 7 0(C C 2 )alkyl-, phenyl, thienyl, pyrazolyl, imidazolyl, or pyridinyl, wherein said phenyl, thienyl, pyrazolyl, imidazolyl, or pyridinyl is optionally substituted one or two times, independently, by halogen, (C C 6 )alkyl, (C 3 -C 6 )cycloalkyl, (C C 4 )haloalkyl, cyano, -CO(C C 4 )alkyl, -C0 2 H, -C0 2 R 7 , -CONH 2 , -CONHR 7 , -CONR 7 R 8 , -SR 7 , -S0 2 (C C 4 )alkyl,
  • R 6 is phenyl optionally substituted one to three times, independently, by halogen, (d-C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 3 -C 6 )cycloalkyl, (C C 4 )haloalkyl, cyano, -CO(C C 4 )alkyl, -C0 2 H, -C0 2 R 7 , -CONH 2 , -CONHR 7 , -CONR 7 R 8 , H0 2 C(C C 2 )alkyl-, R 7 0 2 C(C C 2 )alkyl-, cyano(Ci-C 2 )alkyl-, -SR 7 , -S0 2 (Ci-C 4 )alkyl, -S0 2 NH 2 , -S0 2 NHR 7 , -S0 2 NR 7 R
  • R 6 is phenyl optionally substituted one or two times, independently, by halogen, (CrC 6 )alkyl, (C 3 -C 6 )cycloalkyl,
  • R 7 R 8 N(Ci-C 2 )alkyl-, -NHCO(Ci-C 4 )alkyl, -NHS0 2 (C C 4 )alkyl, oxo, hydroxyl, -OR 7 , hydroxy(Ci-C 2 )alkyl-, R 7 0(CrC 2 )alkyl-, phenyl, thienyl, pyrazolyl, imidazolyl, or pyridinyl, wherein said phenyl, thienyl, pyrazolyl, imidazolyl, or pyridinyl is optionally substituted one or two times, independently, by halogen, (CrC 6 )alkyl, (C 3 -C 6 )cycloalkyl, (CrC 4 )haloalkyl, cyano, -CO(C C 4 )alkyl, -C0 2 H, -C0 2 R 7 , -CONH 2
  • -S0 2 (Ci-C 4 )alkyl -S0 2 NH 2 , -S0 2 NHR 7 , -S0 2 NR 7 R 8 , nitro, amino, -NHR 7 , -NR 7 R 8 ,
  • R 6 is pyridinyl optionally substituted one or two times, independently, by halogen, (C 1 -C 6 )alkyl, (C 3 -C 6 )cycloalkyl,
  • R 7 R 8 N(Ci-C 2 )alkyl-, -NHCO(Ci-C 4 )alkyl, -NHS0 2 (C C 4 )alkyl, oxo, hydroxyl, -OR 7 , hydroxy(Ci-C 2 )alkyl-, or R 7 0(Ci-C 2 )alkyl-.
  • R 6 is pyridinyl optionally substituted one or two times, independently, by halogen
  • R 6 is methyl, ethyl, oxazol-2-yl, oxazol- 5-yl, 4-methyl-oxazol-2-yl, thiazol-2-yl, 4-trifluoromethyl-thiazol-2-yl, 4-isopropyl-thiazol-2- yl, 5-methyl-thiazol-2-yl, 4-carboxymethyl-thiazol-2-yl, 4-(methoxycarbonyl)methyl-thiazol- 2-yl, 5-carboxy-thiazol-2-yl, 1 ,3,4-thiadiazol-2-yl, pyridin-2-yl, 3-fluoro-pyridin-2-yl,
  • R 6 is 2-fluorophenyl
  • R 7 is (Ci-C 4 )alkyl, aryl, heterocycloalkyl, or heterocycloalkyl(Ci-C 2 )alkyl, wherein said (CrC 4 )alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (Ci-C 4 )alkoxy, amino, (Ci-C 4 )alkylamino,
  • R 7 is (C 1 -C 4 )alkyl, phenyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, or
  • R 7 is methyl, difluoromethyl, trifluoromethyl, ethyl, 2,2,2-trifluoroethyl, isopropyl,
  • R 8 is (Ci-C 4 )alkyl.
  • R 7 is methyl or ethyl.
  • R 7 and R 8 taken together with the nitrogen to which they are attached represent a 5-7 membered heterocyclic ring, optionally containing an additional heteroatom selected from oxygen, nitrogen, and sulfur, wherein said ring is optionally substituted one or two times, independently, by halogen, (C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, amino, (C 1 -C 4 )alkylamino, ((C 1 -C 4 )alkyl)((C 1 -C 4 )alkyl)amino, hydroxyl, oxo, (Ci-C 4 )alkoxy, or (Ci-C 4 )alkoxy(Ci-C 4 )alkyl.
  • R 7 and R 8 taken together with the nitrogen to which they are attached represent pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, or hexahydro- 1 H-1 ,4-diazepinyl, each optionally substituted one or two times, independently, by halogen, (d-C 4 )alkyl, (CrC 4 )haloalkyl, amino, (Ci-C 4 )alkylamino, ((Ci-C 4 )alkyl)((Ci-C 4 )alkyl)amino, hydroxyl, oxo, (Ci-C 4 )alkoxy, or
  • R 7 and R 8 taken together with the nitrogen to which they are attached represent pyrrolidinyl
  • R 1 is (C C 4 )alkyl
  • R 2 is hydrogen
  • R 3 is hydrogen, halogen, (Ci-C 4 )alkyl, (C 1 -C 4 )haloalkyl, (C 3 -C 6 )cycloalkyl, aryl, hydroxyl, hydroxy(C 1 -C 4 )alkyl-, (d-C 4 )alkoxy, (C 1 -C 4 )alkoxy(C 1 -C 4 )alkyl-,
  • R 4 is hydrogen, halogen, (CrC 8 )alkyl, (Ci-C 8 )haloalkyl, (C 3 -C 8 )cycloalkyl, hydroxyl, hydroxy(CrC 8 )alkyl-, (Ci-C 8 )alkoxy, (Ci-C )alkoxy(CrC 8 )alkyl-, (Ci-C 8 )haloalkoxy, (C 3 -C 8 )cycloalkyloxy, (C C 8 )alkylthio-, -S0 2 (Ci-C 4 )alkyl, or -NR 7 R 8 ;
  • R 5 is hydrogen
  • R 4 and R 5 taken together with atoms through which they are connected form a partially saturated 5 or 6 membered ring, optionally containing one or two additional heteroatoms selected from N, O and S, which ring may be unsubstituted or substituted with one to three substituents independently selected from (Ci-C )alkyl, (Ci-C )haloalkyl, hydroxy(Ci-C )alkyl-, (Ci-C )alkoxy, (Ci-C )haloalkoxy, and (Ci-C )alkylthio-;
  • R 6 is (CrCe)alkyl, (C 2 -C 8 )alkenyl, (C 2 -C 8 )alkynyl, (C 3 -C 8 )cycloalkyl, aryl, or heteroaryl, wherein any aryl or heteroaryl group is optionally substituted one to three times, independently, by halogen, (CrC 6 )alkyl, (C 3 -C 6 )cycloalkyl, (Ci-C )haloalkyl, cyano, -CO(C C 4 )alkyl, -C0 2 H, -C0 2 R 7 , -CONH 2 , -CONHR 7 , -CONR 7 R 8 , H0 2 C(C C 2 )alkyl-, R 7 0 2 C(C C 2 )alkyl-, -SR 7 , -S0 2 (C C 4 )alkyl, -S0 2 NH 2 , -
  • R 7 is (CrC 4 )alkyl, aryl, heterocycloalkyl, or heterocycloalkyl(Ci-C 2 )alkyl, wherein said (CrC 4 )alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (CrC 4 )alkoxy, amino, (Ci-C 4 )alkylamino, ((Ci-C 4 )alkyl)((Ci-C 4 )alkyl)amino, -C0 2 H, -C0 2 (CrC 4 )alkyl, -CONH 2 , -CONH(C C 4 )alkyl, or
  • R 8 is (Ci-C 4 )alkyl
  • R 7 and R 8 taken together with the nitrogen to which they are attached represent a 5-7 membered heterocyclic ring, optionally containing an additional heteroatom selected from oxygen, nitrogen, and sulfur, wherein said ring is optionally substituted one or two times, independently, by halogen, (C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, amino,
  • R 1 is methyl
  • R 2 is hydrogen or fluorine
  • R 3 is hydrogen, halogen, (d-C 4 )alkyl, (Ci-C 4 )haloalkyl, phenyl, (CrC 4 )alkoxy,
  • R 4 is hydrogen, halogen, (CrC 8 )alkyl, (Ci-C 8 )haloalkyl, (C 3 -C 8 )cycloalkyl, hydroxyl, hydroxy(CrC 8 )alkyl-, (Ci-C 8 )alkoxy, (Ci-C 4 )alkoxy(CrC 8 )alkyl-, (Ci-C 8 )haloalkoxy, (C 3 -C 8 )cycloalkyloxy, (Ci-C 8 )alkylthio-, (Ci-C 8 )haloalkylthio-, -S0 2 (CrC 4 )alkyl, amino, (Ci-C 4 )alkylamino, (Ci-C 4 )haloalkylamino, ((Ci-C 4 )alkyl)((Ci-C 4 )alkyl)amino,
  • R 5 is hydrogen
  • R 6 is phenyl optionally substituted one to three times, independently, by halogen, (C C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 3 -C 6 )cycloalkyl, (C C 4 )haloalkyl, cyano, -CO(Ci-C 4 )alkyl, -C0 2 H, -C0 2 R 7 , -CONH 2 , -CONHR 7 , -CONR 7 R 8 , H0 2 C(C C 2 )alkyl-, R 7 0 2 C(CrC 2 )alkyl-, cyano(Ci-C 2 )alkyl-, -SR 7 , -S0 2 (Ci-C 4 )alkyl, -S0 2 NH 2 , -S0 2 NHR 7 , -S0 2 NR 7 R 8 , nitro,
  • R 7 R 8 N(Ci-C 2 )alkyl-, triazolyl(C C 2 )alkyl-, -NHCO(C C 4 )alkyl, -NHS0 2 (Ci-C 4 )alkyl, oxo, hydroxyl, -OR 7 , hydroxy(Ci-C 2 )alkyl-, R 7 0(CrC 2 )alkyl-, phenyl, thienyl, pyrazolyl, imidazolyl, oxazolyl, thiazolyl, or pyridinyl, wherein said phenyl, thienyl, pyrazolyl, imidazolyl, oxazolyl, thiazolyl, or pyridinyl is optionally substituted one or two times, independently, by halogen, (d-C 6 )alkyl, (C 3 -C 6 )cycloalkyl, (CrC 4 )haloal
  • R 7 is (C 1 -C 4 )alkyl, phenyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, or pyrrolidinyl(C 1 -C 2 )alkyl, piperidinyl(C 1 -C 2 )alkyl, morpholinyl(C 1 -C 2 )alkyl, thiomorpholinyl(C 1 -C 2 )alkyl, or piperazinyl(C 1 -C 2 )alkyl, wherein said (C 1 -C 4 )alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl,
  • R 8 is methyl or ethyl
  • R 7 and R 8 taken together with the nitrogen to which they are attached represent pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, or hexahydro-1 H-1 ,4- diazepinyl, each optionally substituted one or two times, independently, by halogen, (CrC 4 )alkyl, (Ci-C 4 )haloalkyl, amino, (Ci-C 4 )alkylamino, ((Ci-C 4 )alkyl)((Ci-C 4 )alkyl)amino, hydroxyl, oxo, (Ci-C 4 )alkoxy, or (Ci-C 4 )alkoxy(Ci-C 4 )alkyl.
  • R 1 is methyl
  • R 2 is hydrogen or fluorine
  • R 3 is hydrogen, halogen, (C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, phenyl, (C 1 -C 4 )alkoxy, (C C 4 )alkylthio-, or ((C 1 -C 4 )alkyl)((C 1 -C 4 )alkyl)amino;
  • R 4 is hydrogen, halogen, (C 1 -C 8 )alkyl, (C 1 -C 8 )haloalkyl, (C 3 -C 8 )cycloalkyl, hydroxyl, hydroxy(CrC 8 )alkyl-, (Ci-C 8 )alkoxy, (Ci-C 4 )alkoxy(CrC 8 )alkyl-, (Ci-C 8 )haloalkoxy, (C 3 -C 8 )cycloalkyloxy, (Ci-C 8 )alkylthio-, (Ci-C 8 )haloalkylthio-, -S0 2 (CrC 4 )alkyl, amino, (Ci-C 4 )alkylamino, (Ci-C 4 )haloalkylamino, ((Ci-C 4 )alkyl)((Ci-C 4 )alkyl)amino,
  • R 5 is hydrogen
  • R 6 is pyridinyl optionally substituted one or two times, independently, by halogen, (C C 6 )alkyl, (C 3 -C 6 )cycloalkyl, (Ci-C 4 )haloalkyl, cyano, -CO(C C 4 )alkyl, -C0 2 H, -C0 2 R 7 , -CONH 2 , -CONHR 7 , -CONR 7 R 8 , H0 2 C(CrC 2 )alkyl-, R 7 0 2 C(Ci-C 2 )alkyl-, -SR 7 ,
  • -S0 2 (d-C 4 )alkyl -S0 2 NH 2 , -S0 2 NHR 7 , -S0 2 NR 7 R 8 , nitro, amino, -NHR 7 , -NR 7 R 8 , amino(C C 2 )alkyl-, R 7 HN(C C 2 )alkyl-, R 7 R 8 N(C C 2 )alkyl-, -NHCO(d-C 4 )alkyl,
  • R 7 is (C 1 -d)alkyl, phenyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, or pyrrolidinyl(d-C 2 )alkyl, piperidinyl(d-C 2 )alkyl, morpholinyl(d-d)alkyl, thiomorpholinyl(Ci-C 2 )alkyl, or piperazinyl(d-C 2 )alkyl, wherein said (d-d)alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl,
  • (d-d)alkoxy amino, (d-d)alkylamino, ((d-C 4 )alkyl)((d-C 4 )alkyl)amino, -C0 2 H, -C0 2 (Ci-d)alkyl, -CONH 2 , -CONH(d-d)alkyl, or -CON((Ci-d)alkyl)((d-d)alkyl); and wherein any pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, or piperazinyl is optionally substituted by (d-d)alkyl; and
  • R 8 is methyl or ethyl
  • R 7 and R 8 taken together with the nitrogen to which they are attached represent pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, or hexahydro-1 H-1 ,4- diazepinyl, each optionally substituted one or two times, independently, by halogen, (d-d)alkyl, (d-d)haloalkyl, amino, (d-d)alkylamino, ((d-C 4 )alkyl)((d-C 4 )alkyl)amino, hydroxyl, oxo, (d-d)alkoxy, or (d-C 4 )alkoxy(d-C 4 )alkyl.
  • Representative compounds of this invention include the compounds of Examples
  • the compounds according to Formula I may contain one or more asymmetric center (also referred to as a chiral center) and may, therefore, exist as individual enantiomers, diastereomers, or other stereoisomeric forms, or as mixtures thereof.
  • Chiral centers such as chiral carbon atoms, may also be present in a substituent such as an alkyl group.
  • the stereochemistry of a chiral center present in Formula I, or in any chemical structure illustrated herein, is not specified the structure is intended to encompass all individual stereoisomers and all mixtures thereof.
  • compounds according to Formula I containing one or more chiral center may be used as racemic mixtures, enantiomerically enriched mixtures, or as enantiomerically pure individual stereoisomers.
  • Individual stereoisomers of a compound according to Formula I which contain one or more asymmetric center may be resolved by methods known to those skilled in the art. For example, such resolution may be carried out (1 ) by formation of diastereoisomeric salts, complexes or other derivatives; (2) by selective reaction with a stereoisomer- specific reagent, for example by enzymatic oxidation or reduction; or (3) by gas-liquid or liquid chromatography in a chiral environment, for example, on a chiral support such as silica with a bound chiral ligand or in the presence of a chiral solvent.
  • stereoisomers may be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer to the other by asymmetric transformation.
  • polymorphism i.e. the capacity to occur in different crystalline forms. These different crystalline forms are typically known as "polymorphs.” It is to be understood that when named or depicted by structure, the disclosed compound, or solvates (particularly, hydrates) thereof, also include all polymorphs thereof. Polymorphs have the same chemical composition but differ in packing, geometrical arrangement, and other descriptive properties of the crystalline solid state. Polymorphs, therefore, may have different physical properties such as shape, density, hardness, deformability, stability, and dissolution properties. Polymorphs typically exhibit different melting points, IR spectra, and X-ray powder diffraction patterns, which may be used for identification. One of ordinary skill in the art will appreciate that different polymorphs may be produced, for example, by changing or adjusting the conditions used in crystallizing/recrystallizing the compound.
  • solvates of the compounds of the invention, or salts thereof, that are in crystalline form may involve nonaqueous solvents such as ethanol, isopropanol, DMSO, acetic acid, ethanolamine, and ethyl acetate, or they may involve water as the solvent that is incorporated into the crystalline lattice.
  • Solvates wherein water is the solvent that is incorporated into the crystalline lattice are typically referred to as "hydrates.” Hydrates include stoichiometric hydrates as well as compositions containing variable amounts of water. The invention includes all such solvates.
  • the compounds of this invention are bases, wherein a desired salt form may be prepared by any suitable method known in the art, including treatment of the free base with an inorganic acid, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like, or with an organic acid, such as acetic acid, trifluoroacetic acid, maleic acid, succinic acid, mandelic acid, fumaric acid, malonic acid, pyruvic acid, oxalic acid, glycolic acid, salicylic acid, pyranosidyl acid, such as glucuronic acid or galacturonic acid, alpha-hydroxy acid, such as citric acid or tartaric acid, amino acid, such as aspartic acid or glutamic acid, aromatic acid, such as benzoic acid or cinnamic acid, sulfonic acid, such as p-toluenesul
  • an inorganic acid such as hydrochloric acid, hydrobromic acid, sulfuric acid
  • Examples of pharmaceutically acceptable salts include sulfates, pyrosulfates, bisulfates, sulfites, bisulfites, phosphates, chlorides, bromides, iodides, acetates, propionates, decanoates, caprylates, acrylates, formates, isobutyrates, caproates, heptanoates, propiolates, oxalates, malonates succinates, suberates, sebacates, fumarates, maleates, butyne-1 ,4-dioates, hexyne-1 ,6- dioates, benzoates, chlorobenzoates, methylbenzoates, dinitrobenzoates,
  • hydroxybenzoates methoxybenzoates, phthalates, phenylacetates, phenylpropionates, phenylbutrates, citrates, lactates, ⁇ -hydroxybutyrates, glycollates, tartrates mandelates, and sulfonates, such as xylenesulfonates, methanesulfonates, propanesulfonates, naphthalene-1 -sulfonates and naphthalene-2-sulfonates.
  • Salts of the disclosed compounds containing a carboxylic acid or other acidic functional group can be prepared by reacting with a suitable base.
  • a suitable base Such a
  • pharmaceutically acceptable salt may be made with a base which affords a
  • pharmaceutically acceptable cation which includes alkali metal salts (especially sodium and potassium), alkaline earth metal salts (especially calcium and magnesium), aluminum salts and ammonium salts, as well as salts made from physiologically acceptable organic bases such as trimethylamine, triethylamine, morpholine, pyridine, piperidine, picoline, dicyclohexylamine, ⁇ /, ⁇ /'-dibenzylethylenediamine, 2-hydroxyethylamine, bis-(2- hydroxyethyl)amine, tri-(2-hydroxyethyl)amine, procaine, dibenzylpiperidine,
  • alkali metal salts especially sodium and potassium
  • alkaline earth metal salts especially calcium and magnesium
  • aluminum salts and ammonium salts as well as salts made from physiologically acceptable organic bases such as trimethylamine, triethylamine, morpholine, pyridine, piperidine, picoline, dicyclohexylamine, ⁇ /, ⁇ /'-di
  • dehydroabietylamine /V,/V'-i)/sdehydroabietylamine, glucamine, /V-methylglucamine, collidine, quinine, quinoline, and basic amino acid such as lysine and arginine.
  • the corresponding free base form of that compound may be prepared by any suitable method known to the art, including treatment of the salt with an inorganic or organic base, suitably an inorganic or organic base having a higher pK a than the free base form of the compound.
  • the corresponding free acid form of that compound may be prepared by any suitable method known to the art, including treatment of the salt with an inorganic or organic acid, suitably an inorganic or organic acid having a lower pK a than the free acid form of the compound.
  • the compounds of Formula I may be obtained by using synthetic procedures illustrated in the Schemes below or by drawing on the knowledge of a skilled organic chemist.
  • the synthesis provided in these Schemes are applicable for producing compounds of the invention having a variety of different R 1 , R 2 , R 3 , R 4 , R 5 , and R 6 groups employing appropriate precursors, which are suitably protected if needed, to achieve compatibility with the reactions outlined herein. Subsequent deprotection, where needed, affords compounds of the nature generally disclosed. While the Schemes are shown with compounds only of Formula I, they are illustrative of processes that may be used to make the compounds of the invention.
  • the compounds of Formula I can be prepared under a variety of conditions by sequential reaction of an aryl amine (e.g., Ar-NH-R 5 ) and an R 6 -amine (e.g., NH 2 -Ph-R) with an activated 1 ,3,5-triazine.
  • an aryl amine e.g., Ar-NH-R 5
  • an R 6 -amine e.g., NH 2 -Ph-R
  • the invention also includes various deuterated forms of the compounds of Formula I.
  • Each available hydrogen atom attached to a carbon atom may be
  • deuterated alkyl group amines may be prepared by conventional techniques (see for example: methyl-c/3-amine available from Aldrich Chemical Co., Milwaukee, Wl, Cat. No.489, 689-2). Employing such compounds according to Schemes 1 -3 will allow for the preparation of compounds of Formula I in which various hydrogen atoms are replaced with a deuterium atom.
  • the present invention is directed to a method of inhibiting TNNI3K which comprises contacting the kinase with a compound of Formula I or a salt thereof, particularly a pharmaceutically acceptable salt thereof.
  • This invention is also directed to a method of treatment of a TNNI3K-mediated disease or disorder comprising administering an effective amount of the compound of Formula I or a salt thereof, particularly a pharmaceutically acceptable salt thereof, to a patient, specifically a human, in need thereof.
  • patient refers to a human or other mammal.
  • this invention is directed to a method of inhibiting TNNI3K activity, comprising contacting the kinase with an effective amount of a compound of Formula I or a pharmaceutically acceptable salt thereof.
  • TNNI3K activity may be inhibited in mammalian cardiac tissue by administering to a patient in need thereof, an effective amount a compound of Formula I or a pharmaceutically acceptable salt thereof.
  • the compounds of this invention may be particularly useful for treatment of
  • TNNI3K-mediated diseases or disorders specifically by inhibition of TNNI3K activity, where such diseases or disorders are selected from heart failure, particularly congestive heart failure; cardiac hypertrophy; and heart failure or congestive heart failure resulting from cardiac hypertrophy.
  • the compounds of this invention may also be useful for the treatment of heart failure or congestive heart failure resulting from myocardial ischemia or myocardial infarction.
  • a therapeutically "effective amount” is intended to mean that amount of a compound that, when administered to a patient in need of such treatment, is sufficient to effect treatment, as defined herein.
  • a therapeutically effective amount of a compound of Formula I, or a pharmaceutically acceptable salt thereof is a quantity of an inventive agent that, when administered to a human in need thereof, is sufficient to modulate or inhibit the activity of TNNI3K such that a disease condition which is mediated by that activity is reduced, alleviated or prevented.
  • the amount of a given compound that will correspond to such an amount will vary depending upon factors such as the particular compound (e.g., the potency (pXC 5 o), efficacy (EC 5 o), and the biological half-life of the particular compound), disease condition and its severity, the identity (e.g., age, size and weight) of the patient in need of treatment, but can nevertheless be routinely determined by one skilled in the art.
  • the particular compound e.g., the potency (pXC 5 o), efficacy (EC 5 o), and the biological half-life of the particular compound
  • disease condition and its severity e.g., the identity of the patient in need of treatment, but can nevertheless be routinely determined by one skilled in the art.
  • duration of treatment and the time period of administration (time period between dosages and the timing of the dosages, e.g., before/with/after meals) of the compound will vary according to the identity of the mammal in need of treatment (e.g., weight), the particular compound and its properties (e.g., pharmaceutical characteristics), disease or condition and its severity and the specific composition and method being used, but can nevertheless be determined by one of skill in the art.
  • Treating is intended to mean at least the mitigation of a disease condition in a patient, where the disease condition is caused or mediated by TNNI3K.
  • the methods of treatment for mitigation of a disease condition include the use of the compounds in this invention in any conventionally acceptable manner, for example for prevention, retardation, prophylaxis, therapy or cure of a disease.
  • the compounds of Formula I of this invention may be useful for the treatment of heart failure, particularly congestive heart failure.
  • the compounds of Formula I of this invention may be useful for the treatment of cardiac hypertrophy, and heart failure or congestive heart failure resulting from cardiac hypertrophy, myocardial ischemia or myocardial infarction.
  • the compounds of the invention may be administered by any suitable route of administration, including both systemic administration and topical administration.
  • Systemic administration includes oral administration, parenteral administration, transdermal administration, rectal administration, and administration by inhalation.
  • Parenteral administration refers to routes of administration other than enteral
  • transdermal, or by inhalation and is typically by injection or infusion.
  • Parenteral administration includes intravenous, intramuscular, and subcutaneous injection or infusion.
  • Inhalation refers to administration into the patient's lungs whether inhaled through the mouth or through the nasal passages.
  • Topical administration includes application to the skin.
  • the compounds of the invention may be administered once or according to a dosing regimen wherein a number of doses are administered at varying intervals of time for a given period of time. For example, doses may be administered one, two, three, or four times per day. Doses may be administered until the desired therapeutic effect is achieved or indefinitely to maintain the desired therapeutic effect. Suitable dosing regimens for a compound of the invention depend on the pharmacokinetic properties of that compound, such as absorption, distribution, and half-life, which can be determined by the skilled artisan.
  • suitable dosing regimens including the duration such regimens are administered, for a compound of the invention depend on the condition being treated, the severity of the condition being treated, the age and physical condition of the patient being treated, the medical history of the patient to be treated, the nature of concurrent therapy, the desired therapeutic effect, and like factors within the knowledge and expertise of the skilled artisan. It will be further understood by such skilled artisans that suitable dosing regimens may require adjustment given an individual patient's response to the dosing regimen or over time as individual patient needs change.
  • Treatment of TNNI3K-mediated disease conditions may be achieved using the compounds of this invention as a monotherapy, or in dual or multiple combination therapy, such as in combination with other cardiovascular agents, for example, in combination with one or more of the following agents: a beta-blocker, an ACE inhibitor, an angiotensin receptor blocker (ARB), a calcium channel blocker, a diuretic, a renin inhibitor, a centrally acting antihypertensive, a dual ACE/NEP inhibitor, an aldosterone synthase inhibitor, and an aldosterone-receptor antagonist, which are administered in effective amounts as is known in the art.
  • suitable beta blockers include timolol (such as BLOCARDEN ), carteolol (such as CARTROLTM), carvedilol (such as COREGTM), nadolol (such as
  • CORGARDTM propanolol (such as INNOPRAN XLTM), betaxolol (such as KERLONETM), penbutolol (such as LEVATOLTM), metoprolol (such as LOPRESSORTM and TOPROL- XLTM), atenolol (such as TENORMINTM), pindolol (such as VISKENTM), bisoprolol, bucindolol, esmolol, acebutolol, labetalol, nebivolol, celiprolol, sotalol, and oxprenolol.
  • propanolol such as INNOPRAN XLTM
  • betaxolol such as KERLONETM
  • penbutolol such as LEVATOLTM
  • metoprolol such as LOPRESSORTM and TOPROL- XLTM
  • atenolol such
  • ACE inhibitors examples include alacepril, benazepril, benazaprilat, captopril, ceronapril, cilazapril, delapril, enalapril, enalaprilat, fosinopril, lisinopril, moexipiril, moveltopril, perindopril, quinapril, quinaprilat, ramipril, ramiprilat, spirapril, temocapril, trandolapril, and zofenopril.
  • Preferred ACE inhibitors are benazepril, enalpril, lisinopril, and ramipril.
  • angiotensin receptor blockers examples include candesartan, eprosartan, irbesartan, losartan, olmesartan, tasosartan, telmisartan, and valsartan.
  • Suitable calcium channel blockers include dihydropyridines (DHPs) and non- DHPs.
  • DHPs include amlodipine, felodipine, ryosidine, isradipine, lacidipine, nicardipine, nifedipine, nigulpidine, niludipine, nimodiphine, nisoldipine, nitrendipine, and nivaldipine, and their pharmaceutically acceptable salts.
  • Suitable non-DHPs are flunarizine, prenylamine, diltiazem, fendiline, gallopamil, mibefradil, anipamil, tiapamil, and verampimil, and their pharmaceutically acceptable salts.
  • a suitable diuretic is a thiazide derivative selected from amiloride, chlorothiazide, hydrochlorothiazide,
  • a suitable renin inhibitor is aliskiren.
  • suitable centrally acting antiphypertensives include clonidine, guanabenz, guanfacine and methyldopa.
  • suitable dual ACE/NEP inhibitors include omapatrilat, fasidotril, and fasidotrilat.
  • suitable aldosterone synthase inhibitors include anastrozole, fadrozole, and exemestane.
  • suitable aldosterone-receptor antagonists include spironolactone and eplerenone.
  • the invention further includes the use of compounds of the invention as an active therapeutic substance, in particular in the treatment of diseases mediated by TNNI3K.
  • the invention includes the use of compounds of the invention in the treatment of heart failure, particularly congestive heart failure; cardiac hypertrophy; heart failure or congestive heart failure resulting from cardiac hypertrophy; and heart failure or congestive heart failure resulting from myocardial ischemia or myocardial infarction.
  • the invention includes the use of compounds of the invention in the manufacture of a medicament for use in the treatment of the above disorders.
  • the compounds of the invention will normally, but not necessarily, be formulated into a pharmaceutical composition prior to administration to a patient. Accordingly, in another aspect the invention is directed to pharmaceutical compositions comprising a compound of the invention and a pharmaceutically-acceptable excipient.
  • compositions of the invention may be prepared and packaged in bulk form wherein an effective amount of a compound of the invention can be extracted and then given to the patient such as with powders, syrups, and solutions for injection.
  • the pharmaceutical compositions of the invention may be prepared and packaged in unit dosage form.
  • a dose of the pharmaceutical composition contains at least a therapeutically effective amount of a compound of this invention (i.e., a compound of Formula I or a salt, particularly a pharmaceutically acceptable salt, thereof).
  • the pharmaceutical compositions may contain from 1 mg to 1000 mg of a compound of this invention.
  • compositions of the invention typically contain one compound of the invention. However, in certain embodiments, the pharmaceutical compositions of the invention contain more than one compound of the invention. In addition, the pharmaceutical compositions of the invention may optionally further comprise one or more additional pharmaceutically active compounds.
  • pharmaceutically-acceptable excipient means a material, composition or vehicle involved in giving form or consistency to the composition.
  • Each excipient must be compatible with the other ingredients of the pharmaceutical composition when commingled such that interactions which would substantially reduce the efficacy of the compound of the invention when administered to a patient and interactions which would result in pharmaceutical compositions that are not pharmaceutically-acceptable are avoided.
  • each excipient must of course be of sufficiently high purity to render it pharmaceutically-acceptable.
  • the compounds of the invention and the pharmaceutically-acceptable excipient or excipients will typically be formulated into a dosage form adapted for administration to the patient by the desired route of administration.
  • Conventional dosage forms include those adapted for (1 ) oral administration such as tablets, capsules, caplets, pills, troches, powders, syrups, elixirs, suspensions, solutions, emulsions, sachets, and cachets; (2) parenteral administration such as sterile solutions, suspensions, and powders for reconstitution; (3) transdermal administration such as transdermal patches; (4) rectal administration such as suppositories; (5) inhalation such as aerosols and solutions; and (6) topical administration such as creams, ointments, lotions, solutions, pastes, sprays, foams, and gels.
  • Suitable pharmaceutically-acceptable excipients will vary depending upon the particular dosage form chosen.
  • suitable pharmaceutically-acceptable excipients may be chosen for a particular function that they may serve in the composition.
  • certain pharmaceutically-acceptable excipients may be chosen for their ability to facilitate the production of uniform dosage forms.
  • Certain pharmaceutically- acceptable excipients may be chosen for their ability to facilitate the production of stable dosage forms.
  • Certain pharmaceutically-acceptable excipients may be chosen for their ability to facilitate the carrying or transporting the compound or compounds of the invention once administered to the patient from one organ, or portion of the body, to another organ, or portion of the body.
  • Certain pharmaceutically-acceptable excipients may be chosen for their ability to enhance patient compliance.
  • Suitable pharmaceutically-acceptable excipients include the following types of excipients: diluents, fillers, binders, disintegrants, lubricants, glidants, granulating agents, coating agents, wetting agents, solvents, co-solvents, suspending agents, emulsifiers, sweeteners, flavoring agents, flavor masking agents, coloring agents, anti-caking agents, humectants, chelating agents, plasticizers, viscosity increasing agents, antioxidants, preservatives, stabilizers, surfactants, and buffering agents.
  • excipients include the following types of excipients: diluents, fillers, binders, disintegrants, lubricants, glidants, granulating agents, coating agents, wetting agents, solvents, co-solvents, suspending agents, emulsifiers, sweeteners, flavoring agents, flavor masking agents, coloring agents, anti-caking agents, humectants,
  • Skilled artisans possess the knowledge and skill in the art to enable them to select suitable pharmaceutically-acceptable excipients in appropriate amounts for use in the invention.
  • resources that are available to the skilled artisan which describe pharmaceutically-acceptable excipients and may be useful in selecting suitable pharmaceutically-acceptable excipients. Examples include Remington's Pharmaceutical Sciences (Mack Publishing Company), The Handbook of Pharmaceutical Additives (Gower Publishing Limited), and The Handbook of Pharmaceutical Excipients (the American Pharmaceutical Association and the Pharmaceutical Press).
  • compositions of the invention are prepared using techniques and methods known to those skilled in the art. Some of the methods commonly used in the art are described in Remington's Pharmaceutical Sciences (Mack Publishing
  • the invention is directed to a solid oral dosage form such as a tablet or capsule comprising an effective amount of a compound of the invention and a diluent or filler.
  • Suitable diluents and fillers include lactose, sucrose, dextrose, mannitol, sorbitol, starch (e.g. corn starch, potato starch, and pre-gelatinized starch), cellulose and its derivatives (e.g. microcrystalline cellulose), calcium sulfate, and dibasic calcium phosphate.
  • the oral solid dosage form may further comprise a binder. Suitable binders include starch (e.g.
  • the oral solid dosage form may further comprise a disintegrant. Suitable disintegrants include crospovidone, sodium starch glycolate, croscarmelose, alginic acid, and sodium carboxymethyl cellulose.
  • the oral solid dosage form may further comprise a lubricant. Suitable lubricants include stearic acid, magnesium stearate, calcium stearate, and talc.
  • Step 1 3,3'-[(6-chloro-1 ,3,5-triazine-2,4-diyl)diimino]i)/s(/ ⁇ /-methylbenzenesulfonamide)
  • 2,4,6-trichloro-1 ,3,5-triazine 0.050 mg, 0.271 mmol
  • Step 2 3,3'-(1 ,3,5-triazine-2,4-diyldiimino)i)/s(/ ⁇ /-methylbenzenesulfonamide)
  • Step 1 4-chloro-/V-(4-chlorophenyl)-1 ,3,5-triazin-2-amine
  • reaction mixture was filtered and purified by mass directed autoprep HPLC (Waters, Sunfire prep C18 OBD, 30 x 150 mm, 40-90% CH 3 CN/water plus 0.1 % TFA). Concentration of the appropriate fractions yielded 3-( ⁇ 4-[(4-chlorophenyl)amino]-1 ,3,5-triazin-2-yl ⁇ amino)-4- (dimethylamino)-N-methylbenzenesulfonamide trifluoroacetate (0.045 g, 20% yield), as a brown solid.
  • Tablets are prepared using conventional methods and are formulated as follows
  • Capsules are prepared using conventional methods and are formulated as follows:
  • hTNNI3K His-MBP-TEV-Full length human TNNI3K (hTNNI3K) was expressed in Baculokinase system and purified from amylase affinity column followed by Superdex200.
  • the preparation of this fluorescent ligand is disclosed in U.S. Provisional Patent Application No.
  • the other buffer components including MgCI 2 (Catalog Number M1028), Bis-Tris (Catalog Number B7535), DTT (Catalog Number D9779) and Chaps (Catalog Number C3023) were purchased from Sigma-Aldrich.
  • a fluorescent polarization assay was used to determine does response of compound inhibition on hTNNI3K ATP binding.
  • the binding of 5-( ⁇ [2-( ⁇ [3-( ⁇ 4-[(5-hydroxy- 2-methylphenyl)amino]-2-pyrimidinyl ⁇ amino)phenyl]carbonyl ⁇ amino)ethyl]amino ⁇ carbonyl)- 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid to the hTNNI3K ATP binding pocket results in increase of fluorescent polarization and the displacement of 5-( ⁇ [2-( ⁇ [3-( ⁇ 4-[(5- hydroxy-2-methylphenyl)amino]-2-pyrimidinyl ⁇ amino)phenyl]carbonyl ⁇ amino)ethyl]amino ⁇ carbonyl)-2-(6-hydroxy-3-oxo-3/-/-xanthen-9-yl)benzoic acid by a competitive compound leads to fluorescent
  • Solution 1 Ten (10) ml. of a 5 nM 5-( ⁇ [2-( ⁇ [3-( ⁇ 4-[(5-hydroxy-2-methylphenyl) amino]-2-pyrimidinyl ⁇ amino)phenyl]carbonyl ⁇ amino)ethyl]amino ⁇ carbonyl)-2-(6-hydroxy-3- oxo-3H-xanthen-9-yl)benzoic acid solution (Solution 1 ) was prepared by mixing 5 ⁇ _ of 1 M DTT and 80 ⁇ _ of 10% (w/v) Chaps and 5 ⁇ _ of a 10 ⁇ 5-( ⁇ [2-( ⁇ [3-( ⁇ 4-[(5-hydroxy-2- methylphenyl)amino]-2-pyrimidinyl ⁇ amino)phenyl]carbonyl ⁇ amino) ethyl]amino ⁇ carbonyl)- 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid stock solution into
  • Solution 2 was formed by mixing 53.8 ⁇ _ of 2.6 ⁇ hTNNI3K with a 6946.2 ⁇ _ aliquot of Solution 1 (the above 5-( ⁇ [2-( ⁇ [3-( ⁇ 4-[(5-hydroxy-2-methylphenyl)amino]-2- pyrimidinyl ⁇ amino)phenyl]carbonyl ⁇ amino)ethyl]amino ⁇ carbonyl)-2-(6-hydroxy-3-oxo-3/-/- xanthen-9-yl)benzoic acid solution) to make up a 7 mL of mixture of hTNNI3K and 5-( ⁇ [2- ( ⁇ [3-( ⁇ 4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl ⁇ amino)phenyl]carbonyl
  • nl_ of inhibitors in DMSO were stamped into a 384-well low volume Greiner black plate, followed by addition of 5 ⁇ _ of Solution 1 to column 18 and 5 ⁇ _ Solution 2 to columns 1-17 and 19-24 of the plate. The plate was then spun at 500 rpm for 30 seconds and incubated at rt for 60 min. After that, the fluorescent polarization was measured on Analyst (ex/em: 485/530 nm, Dichroic: 505).
  • the pXC 50 s are averaged to determine a mean value, for a minimum of 2 experiments. As determined using the above method, the compounds of Example 1 -12 exhibited a pXC 50 greater than or equal to approximately 6.0. For instance, the compounds of Example 4 and Example 6 each inhibited hTNNI3K in the above method with a mean pXC 50 of approximately 7.3.

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Abstract

L'invention porte sur des composés ayant la formule (I) : dans laquelle R1, R2, R3, R4, R5 et R6 sont tels que définis présentement, et sur des procédés de fabrication et d'utilisation de ces composés.
PCT/US2011/020809 2010-01-13 2011-01-11 Composés et procédés WO2011088031A1 (fr)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT511441A4 (de) * 2011-09-21 2012-12-15 Univ Wien Tech Triazin-derivate als differenzierungsbeschleuniger
US9126944B2 (en) 2013-02-28 2015-09-08 Bristol-Myers Squibb Company Phenylpyrazole derivatives as potent ROCK1 and ROCK2 inhibitors
US9828345B2 (en) 2013-02-28 2017-11-28 Bristol-Myers Squibb Company Phenylpyrazole derivatives as potent ROCK1 and ROCK2 inhibitors

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080242681A1 (en) * 2004-01-22 2008-10-02 Altana Pharma Ag N-4-(6-(Hetero)Aryl-Pyrimidin-4-Ylaminophenyl)-Benzenesulfonamides as Kinase Inhibitors
WO2009028891A2 (fr) * 2007-08-31 2009-03-05 Hanall Pharmaceutical Company. Ltd Composé de 1,3,5-triazine-2,4,6-triamine ou son sel pharmaceutiquement acceptable, et composition pharmaceutique le comprenant

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080242681A1 (en) * 2004-01-22 2008-10-02 Altana Pharma Ag N-4-(6-(Hetero)Aryl-Pyrimidin-4-Ylaminophenyl)-Benzenesulfonamides as Kinase Inhibitors
WO2009028891A2 (fr) * 2007-08-31 2009-03-05 Hanall Pharmaceutical Company. Ltd Composé de 1,3,5-triazine-2,4,6-triamine ou son sel pharmaceutiquement acceptable, et composition pharmaceutique le comprenant

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT511441A4 (de) * 2011-09-21 2012-12-15 Univ Wien Tech Triazin-derivate als differenzierungsbeschleuniger
AT511441B1 (de) * 2011-09-21 2012-12-15 Univ Wien Tech Triazin-derivate als differenzierungsbeschleuniger
US9611457B2 (en) 2011-09-21 2017-04-04 Technische Universitaet Wien Triazine derivatives as differentiation catalysts
US9126944B2 (en) 2013-02-28 2015-09-08 Bristol-Myers Squibb Company Phenylpyrazole derivatives as potent ROCK1 and ROCK2 inhibitors
US9458110B2 (en) 2013-02-28 2016-10-04 Bristol-Myers Squibb Company Phenylpyrazole derivatives as potent ROCK1 and ROCK2 inhibitors
US9828345B2 (en) 2013-02-28 2017-11-28 Bristol-Myers Squibb Company Phenylpyrazole derivatives as potent ROCK1 and ROCK2 inhibitors

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