WO2011086447A3 - Procédé de fermentation pour la préparation de protéines hétérologues recombinantes - Google Patents

Procédé de fermentation pour la préparation de protéines hétérologues recombinantes Download PDF

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Publication number
WO2011086447A3
WO2011086447A3 PCT/IB2011/000022 IB2011000022W WO2011086447A3 WO 2011086447 A3 WO2011086447 A3 WO 2011086447A3 IB 2011000022 W IB2011000022 W IB 2011000022W WO 2011086447 A3 WO2011086447 A3 WO 2011086447A3
Authority
WO
WIPO (PCT)
Prior art keywords
heterologous proteins
preparation
fermentation process
recombinant heterologous
vector
Prior art date
Application number
PCT/IB2011/000022
Other languages
English (en)
Other versions
WO2011086447A2 (fr
Inventor
Prakash Kotwal
Sampali Banerjee
Sriram Padmanabhan
Original Assignee
Lupin Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lupin Limited filed Critical Lupin Limited
Publication of WO2011086447A2 publication Critical patent/WO2011086447A2/fr
Publication of WO2011086447A3 publication Critical patent/WO2011086447A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de fermentation à densité de cellules élevée pour la production de protéines hétérologues dans un système d'expression de E. coli. L'invention concerne en outre le procédé pour la production de protéines hétérologues dans E. coli en utilisant un vecteur pBAD24T7g10. L'invention concerne en outre le vecteur pBAD24T7g10. L'invention concerne en outre le procédé de préparation du vecteur pBAD24T7g10. La fermentation peut être effectuée en utilisant des vecteurs de E. coli K12.
PCT/IB2011/000022 2010-01-12 2011-01-10 Procédé de fermentation pour la préparation de protéines hétérologues recombinantes WO2011086447A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN29/KOL/2010 2010-01-12
IN29KO2010 2010-01-12

Publications (2)

Publication Number Publication Date
WO2011086447A2 WO2011086447A2 (fr) 2011-07-21
WO2011086447A3 true WO2011086447A3 (fr) 2011-10-13

Family

ID=44223559

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2011/000022 WO2011086447A2 (fr) 2010-01-12 2011-01-10 Procédé de fermentation pour la préparation de protéines hétérologues recombinantes

Country Status (1)

Country Link
WO (1) WO2011086447A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704051B2 (en) * 2014-12-29 2020-07-07 Agricultural Technology Research Institute Expression element, expression cassette, and vector containing the same
CN114107264A (zh) * 2021-12-07 2022-03-01 武汉新华扬生物股份有限公司 一种发酵生产氨肽酶的方法
CN114015628A (zh) * 2021-12-17 2022-02-08 河南兴华生物技术有限公司 表达pmt蛋白的培养基及培养方法
CN114350588B (zh) * 2021-12-31 2024-03-15 山东新时代药业有限公司 一种rhG-CSF的发酵培养基及其发酵方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079281A1 (fr) * 2000-04-19 2001-10-25 Ovita Limited Pou du mouton, bovicola ovis, traitement par allergene
WO2008147781A2 (fr) * 2007-05-22 2008-12-04 Ls9, Inc. Gènes produisant des hydrocarbures et procédés pour leur utilisation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002345829A1 (en) 2002-06-24 2004-01-06 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf
DE10360483B4 (de) 2003-12-22 2007-11-15 Scil Proteins Gmbh Expressionsvektor und dessen Verwendung
EP1994138B1 (fr) 2006-03-06 2014-04-30 Cadila Healthcare Limited Méthode de préparation du g-csf humain
WO2008096368A2 (fr) 2007-02-05 2008-08-14 Natco Pharma Limited Nouveau procédé de production du facteur g-csf humain de recombinaison

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079281A1 (fr) * 2000-04-19 2001-10-25 Ovita Limited Pou du mouton, bovicola ovis, traitement par allergene
WO2008147781A2 (fr) * 2007-05-22 2008-12-04 Ls9, Inc. Gènes produisant des hydrocarbures et procédés pour leur utilisation

Also Published As

Publication number Publication date
WO2011086447A2 (fr) 2011-07-21

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