WO2011086447A3 - Procédé de fermentation pour la préparation de protéines hétérologues recombinantes - Google Patents
Procédé de fermentation pour la préparation de protéines hétérologues recombinantes Download PDFInfo
- Publication number
- WO2011086447A3 WO2011086447A3 PCT/IB2011/000022 IB2011000022W WO2011086447A3 WO 2011086447 A3 WO2011086447 A3 WO 2011086447A3 IB 2011000022 W IB2011000022 W IB 2011000022W WO 2011086447 A3 WO2011086447 A3 WO 2011086447A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- heterologous proteins
- preparation
- fermentation process
- recombinant heterologous
- vector
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La présente invention concerne un procédé de fermentation à densité de cellules élevée pour la production de protéines hétérologues dans un système d'expression de E. coli. L'invention concerne en outre le procédé pour la production de protéines hétérologues dans E. coli en utilisant un vecteur pBAD24T7g10. L'invention concerne en outre le vecteur pBAD24T7g10. L'invention concerne en outre le procédé de préparation du vecteur pBAD24T7g10. La fermentation peut être effectuée en utilisant des vecteurs de E. coli K12.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN29/KOL/2010 | 2010-01-12 | ||
IN29KO2010 | 2010-01-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2011086447A2 WO2011086447A2 (fr) | 2011-07-21 |
WO2011086447A3 true WO2011086447A3 (fr) | 2011-10-13 |
Family
ID=44223559
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2011/000022 WO2011086447A2 (fr) | 2010-01-12 | 2011-01-10 | Procédé de fermentation pour la préparation de protéines hétérologues recombinantes |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2011086447A2 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10704051B2 (en) * | 2014-12-29 | 2020-07-07 | Agricultural Technology Research Institute | Expression element, expression cassette, and vector containing the same |
CN114107264A (zh) * | 2021-12-07 | 2022-03-01 | 武汉新华扬生物股份有限公司 | 一种发酵生产氨肽酶的方法 |
CN114015628A (zh) * | 2021-12-17 | 2022-02-08 | 河南兴华生物技术有限公司 | 表达pmt蛋白的培养基及培养方法 |
CN114350588B (zh) * | 2021-12-31 | 2024-03-15 | 山东新时代药业有限公司 | 一种rhG-CSF的发酵培养基及其发酵方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001079281A1 (fr) * | 2000-04-19 | 2001-10-25 | Ovita Limited | Pou du mouton, bovicola ovis, traitement par allergene |
WO2008147781A2 (fr) * | 2007-05-22 | 2008-12-04 | Ls9, Inc. | Gènes produisant des hydrocarbures et procédés pour leur utilisation |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2002345829A1 (en) | 2002-06-24 | 2004-01-06 | Dr. Reddy's Laboratories Ltd. | Process for preparing g-csf |
DE10360483B4 (de) | 2003-12-22 | 2007-11-15 | Scil Proteins Gmbh | Expressionsvektor und dessen Verwendung |
EP1994138B1 (fr) | 2006-03-06 | 2014-04-30 | Cadila Healthcare Limited | Méthode de préparation du g-csf humain |
WO2008096368A2 (fr) | 2007-02-05 | 2008-08-14 | Natco Pharma Limited | Nouveau procédé de production du facteur g-csf humain de recombinaison |
-
2011
- 2011-01-10 WO PCT/IB2011/000022 patent/WO2011086447A2/fr active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001079281A1 (fr) * | 2000-04-19 | 2001-10-25 | Ovita Limited | Pou du mouton, bovicola ovis, traitement par allergene |
WO2008147781A2 (fr) * | 2007-05-22 | 2008-12-04 | Ls9, Inc. | Gènes produisant des hydrocarbures et procédés pour leur utilisation |
Also Published As
Publication number | Publication date |
---|---|
WO2011086447A2 (fr) | 2011-07-21 |
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