WO2011071359A3 - Covalent modification of proteins for the instantaneous visualisation thereof and subsequent characterisation through mass spectrometry - Google Patents

Covalent modification of proteins for the instantaneous visualisation thereof and subsequent characterisation through mass spectrometry Download PDF

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Publication number
WO2011071359A3
WO2011071359A3 PCT/MX2010/000144 MX2010000144W WO2011071359A3 WO 2011071359 A3 WO2011071359 A3 WO 2011071359A3 MX 2010000144 W MX2010000144 W MX 2010000144W WO 2011071359 A3 WO2011071359 A3 WO 2011071359A3
Authority
WO
WIPO (PCT)
Prior art keywords
visualisation
instantaneous
proteins
covalent modification
dye
Prior art date
Application number
PCT/MX2010/000144
Other languages
Spanish (es)
French (fr)
Other versions
WO2011071359A2 (en
Inventor
Robert Winkler
Marco Arnulfo MATA GÓMEZ
Matthew Thomas Yasui
Original Assignee
Instituto Tecnológico y de Estudios Superiores de Monterrey
Cantu Ortiz, Francisco Javier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Tecnológico y de Estudios Superiores de Monterrey, Cantu Ortiz, Francisco Javier filed Critical Instituto Tecnológico y de Estudios Superiores de Monterrey
Publication of WO2011071359A2 publication Critical patent/WO2011071359A2/en
Publication of WO2011071359A3 publication Critical patent/WO2011071359A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides

Abstract

The invention relates to the covalent modification of proteins for the instantaneous visualisation and subsequent characterisation thereof. The modification is produced by nucleophilic addition of a dye with a functional group, for example a vinyl group that reacts with the amino groups of the protein, preferably the lysine amino acids of the peptide sequence, the dye optionally having at least another functional group, for example a sulphate group that acts as an ionisable functional group to increase the water-solubility of the stain, and subsequently to increase the solubility of the peptides, influencing the mass spectometry ionisation thereof. The dye is preferably Uniblue A.
PCT/MX2010/000144 2009-12-09 2010-12-03 Covalent modification of proteins for the instantaneous visualisation thereof and subsequent characterisation through mass spectrometry WO2011071359A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MXMX/A/2009/013417 2009-12-09
MX2009013417A MX2009013417A (en) 2009-12-09 2009-12-09 Covalent modification of proteins for the instantaneous visualisation thereof and subsequent characterisation through mass spectrometry.

Publications (2)

Publication Number Publication Date
WO2011071359A2 WO2011071359A2 (en) 2011-06-16
WO2011071359A3 true WO2011071359A3 (en) 2011-11-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MX2010/000144 WO2011071359A2 (en) 2009-12-09 2010-12-03 Covalent modification of proteins for the instantaneous visualisation thereof and subsequent characterisation through mass spectrometry

Country Status (2)

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MX (1) MX2009013417A (en)
WO (1) WO2011071359A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103308370B (en) * 2013-05-30 2015-11-25 首都医科大学 Single blue A is as the purposes of protein pre-dyed toner
CN112176038A (en) * 2019-07-01 2021-01-05 申翌生物科技(杭州)有限公司 Buffer composition

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1124198A1 (en) * 1983-05-25 1984-11-15 Институт биологической физики АН СССР Method of binding globular proteins with procionic dyes
WO2001011373A2 (en) * 1999-08-09 2001-02-15 Carnegie Mellon University Difference detection methods using matched multiple dyes
US6319720B1 (en) * 1998-10-13 2001-11-20 Ewald M. Wondrak Process for fast visualization of protein
EP2031382A1 (en) * 2007-08-29 2009-03-04 Agilent Technologies, Inc. On-chip analysis of covalently labelled sample species
US20090223823A1 (en) * 2004-12-06 2009-09-10 National Institute Of Advanced Industrial Science And Technology Method of separating protein, method of staining protein and liquid protein-staining agent and protein-staining kit to be used in these methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1124198A1 (en) * 1983-05-25 1984-11-15 Институт биологической физики АН СССР Method of binding globular proteins with procionic dyes
US6319720B1 (en) * 1998-10-13 2001-11-20 Ewald M. Wondrak Process for fast visualization of protein
WO2001011373A2 (en) * 1999-08-09 2001-02-15 Carnegie Mellon University Difference detection methods using matched multiple dyes
US20090223823A1 (en) * 2004-12-06 2009-09-10 National Institute Of Advanced Industrial Science And Technology Method of separating protein, method of staining protein and liquid protein-staining agent and protein-staining kit to be used in these methods
EP2031382A1 (en) * 2007-08-29 2009-03-04 Agilent Technologies, Inc. On-chip analysis of covalently labelled sample species

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI 9 June 2011 Derwent World Patents Index; AN 1985-139788 *

Also Published As

Publication number Publication date
WO2011071359A2 (en) 2011-06-16
MX2009013417A (en) 2011-06-15

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