WO2011068341A3 - Gene and protein for biosynthesis of tricyclocompounds - Google Patents

Gene and protein for biosynthesis of tricyclocompounds Download PDF

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Publication number
WO2011068341A3
WO2011068341A3 PCT/KR2010/008496 KR2010008496W WO2011068341A3 WO 2011068341 A3 WO2011068341 A3 WO 2011068341A3 KR 2010008496 W KR2010008496 W KR 2010008496W WO 2011068341 A3 WO2011068341 A3 WO 2011068341A3
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WO
WIPO (PCT)
Prior art keywords
gene
protein
tricyclocompounds
biosynthesis
tacrolimus
Prior art date
Application number
PCT/KR2010/008496
Other languages
French (fr)
Korean (ko)
Other versions
WO2011068341A2 (en
Inventor
김재종
임시규
김동환
이금순
유정현
이미옥
임상면
이보미
김상준
차선호
윤여준
Original Assignee
(주)제노텍
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)제노텍 filed Critical (주)제노텍
Publication of WO2011068341A2 publication Critical patent/WO2011068341A2/en
Publication of WO2011068341A3 publication Critical patent/WO2011068341A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention relates to a gene constituting biosynthetic loci used in biosynthesis of tricyclocompounds produced by microorganisms, particularly the Actinomycete sp., tacrolimus (FK506) and dihydrotacrolimus, which is a structural analog thereof, ascomycin (FK520), prolyl-tacrolimus (FK525), FK523 and the like, and a protein which is encoded by the gene. The gene and the protein can be used to produce and chemically modify tacrolimus, ascomycin and materials having the chemical structure related thereto and to reduce impurities.
PCT/KR2010/008496 2009-12-03 2010-11-29 Gene and protein for biosynthesis of tricyclocompounds WO2011068341A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2009-0118854 2009-12-03
KR1020090118854A KR101189475B1 (en) 2009-12-03 2009-12-03 Genes and proteins for biosynthesis of tricyclocompounds

Publications (2)

Publication Number Publication Date
WO2011068341A2 WO2011068341A2 (en) 2011-06-09
WO2011068341A3 true WO2011068341A3 (en) 2011-12-22

Family

ID=44115408

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/008496 WO2011068341A2 (en) 2009-12-03 2010-11-29 Gene and protein for biosynthesis of tricyclocompounds

Country Status (2)

Country Link
KR (1) KR101189475B1 (en)
WO (1) WO2011068341A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2921056T3 (en) * 2016-04-12 2022-08-17 Warp Drive Bio Inc Compositions and methods for the production of compounds
KR102109168B1 (en) * 2018-12-11 2020-05-12 인트론바이오테크놀로지 A novel compound and a pharmaceutical composition for treating fungal infections
CN113939519A (en) * 2018-12-11 2022-01-14 莫尔根生物有限公司 Novel compounds for promoting hair growth and compositions containing them

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR970021308A (en) * 1995-10-26 1997-05-28 이우용 DNA fragments encoding polyketide synthase genes
US6503737B1 (en) * 1998-10-02 2003-01-07 Kosan Biosciences, Inc. Isolated nucleic acids relating to the fkbA gene within the FK-520 polyketide synthase gene cluster
WO2011021036A1 (en) * 2009-08-20 2011-02-24 Biotica Technology Limited Polyketides analogues and methods for their production

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR970021308A (en) * 1995-10-26 1997-05-28 이우용 DNA fragments encoding polyketide synthase genes
US6503737B1 (en) * 1998-10-02 2003-01-07 Kosan Biosciences, Inc. Isolated nucleic acids relating to the fkbA gene within the FK-520 polyketide synthase gene cluster
WO2011021036A1 (en) * 2009-08-20 2011-02-24 Biotica Technology Limited Polyketides analogues and methods for their production

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HAIDEH MOTAMEDI AND ALI SHAFIEE: "The biosynthetic gene cluster for the macrolactone ring of the immunosuppressant FK506", EUR. J. BIOCHEM., vol. 256, 1998, pages 528 - 534, XP000906738, DOI: doi:10.1046/j.1432-1327.1998.2560528.x *
HAIDEH MOTAMEDI ET AL.: "Characterization of methyltransferase and hydroxylase genes involved in the biosynthesis of the immunosuppressants FK506 and FK520", J BACTERIOL., vol. 178, no. 17, September 1996 (1996-09-01), pages 5243 - 5248, XP002137077 *
KAI WU ET AL.: "The FK520 gene cluster of streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units", GENE, vol. 251, 2000, pages 81 - 90, XP004202089, DOI: doi:10.1016/S0378-1119(00)00171-2 *

Also Published As

Publication number Publication date
KR20110062208A (en) 2011-06-10
WO2011068341A2 (en) 2011-06-09
KR101189475B1 (en) 2012-10-15

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