WO2011068340A3 - Synthetic nonionic hydrophilic polymer-based prestained protein size marker - Google Patents

Synthetic nonionic hydrophilic polymer-based prestained protein size marker Download PDF

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Publication number
WO2011068340A3
WO2011068340A3 PCT/KR2010/008476 KR2010008476W WO2011068340A3 WO 2011068340 A3 WO2011068340 A3 WO 2011068340A3 KR 2010008476 W KR2010008476 W KR 2010008476W WO 2011068340 A3 WO2011068340 A3 WO 2011068340A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein size
prestained protein
size marker
nonionic hydrophilic
synthetic nonionic
Prior art date
Application number
PCT/KR2010/008476
Other languages
French (fr)
Korean (ko)
Other versions
WO2011068340A2 (en
Inventor
윤성준
전수연
백형록
강상현
Original Assignee
주식회사 인트론바이오테크놀로지
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 인트론바이오테크놀로지 filed Critical 주식회사 인트론바이오테크놀로지
Publication of WO2011068340A2 publication Critical patent/WO2011068340A2/en
Publication of WO2011068340A3 publication Critical patent/WO2011068340A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a prestained protein size marker, and more particularly, to a prestained protein size marker based on synthetic nonionic hydrophilic polymers. According to the method proposed by the present invention, synthetic nonionic hydrophilic polymers, which can be prepared more easily and which have a simpler molecular structure than conventional proteins, are employed as materials for the prestained protein size marker. Thus, materials for prestained protein size markers can be easily prepared, and processes for producing prestained protein size markers can be easily controlled.
PCT/KR2010/008476 2009-12-01 2010-11-29 Synthetic nonionic hydrophilic polymer-based prestained protein size marker WO2011068340A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090117696A KR101111351B1 (en) 2009-12-01 2009-12-01 Pre-stained protein size marker based on synthetic nonionic hydrophilic polymers
KR10-2009-0117696 2009-12-01

Publications (2)

Publication Number Publication Date
WO2011068340A2 WO2011068340A2 (en) 2011-06-09
WO2011068340A3 true WO2011068340A3 (en) 2011-10-20

Family

ID=44115407

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/008476 WO2011068340A2 (en) 2009-12-01 2010-11-29 Synthetic nonionic hydrophilic polymer-based prestained protein size marker

Country Status (2)

Country Link
KR (1) KR101111351B1 (en)
WO (1) WO2011068340A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110408639A (en) * 2019-08-01 2019-11-05 天德悦（北京）生物科技有限责任公司 Pre-dyed albumen marker and preparation method thereof, application can be exposed
WO2022125555A1 (en) * 2020-12-07 2022-06-16 Bio-Rad Laboratories, Inc. Method and composition for quantifying protein using tagged standards

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449758A (en) * 1993-12-02 1995-09-12 Life Technologies, Inc. Protein size marker ladder
US5616502A (en) * 1995-05-19 1997-04-01 Molecular Probes, Inc. Non-specific protein staining using merocyanine dyes
US20050176157A1 (en) * 2001-12-19 2005-08-11 Elena Vasilyeva Methods for detecting half-antibodies using chip-based gel electrophoresis
US20090200167A1 (en) * 2006-02-10 2009-08-13 Martin Kratzmeier Protein Analysis Using A Polymethine Marker Dye

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449758A (en) * 1993-12-02 1995-09-12 Life Technologies, Inc. Protein size marker ladder
US5616502A (en) * 1995-05-19 1997-04-01 Molecular Probes, Inc. Non-specific protein staining using merocyanine dyes
US20050176157A1 (en) * 2001-12-19 2005-08-11 Elena Vasilyeva Methods for detecting half-antibodies using chip-based gel electrophoresis
US20090200167A1 (en) * 2006-02-10 2009-08-13 Martin Kratzmeier Protein Analysis Using A Polymethine Marker Dye

Also Published As

Publication number Publication date
KR20110061144A (en) 2011-06-09
WO2011068340A2 (en) 2011-06-09
KR101111351B1 (en) 2012-06-12

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