WO2011051738A1 - Incubateur à régulation de température - Google Patents

Incubateur à régulation de température Download PDF

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Publication number
WO2011051738A1
WO2011051738A1 PCT/HU2010/000111 HU2010000111W WO2011051738A1 WO 2011051738 A1 WO2011051738 A1 WO 2011051738A1 HU 2010000111 W HU2010000111 W HU 2010000111W WO 2011051738 A1 WO2011051738 A1 WO 2011051738A1
Authority
WO
WIPO (PCT)
Prior art keywords
plate
nest
board
incubator
boards
Prior art date
Application number
PCT/HU2010/000111
Other languages
English (en)
Inventor
Csaba Niedetzky
János MATKÓ
Original Assignee
Eötvös Lóránd Tudományegyetem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eötvös Lóránd Tudományegyetem filed Critical Eötvös Lóránd Tudományegyetem
Publication of WO2011051738A1 publication Critical patent/WO2011051738A1/fr

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Classifications

    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/34Microscope slides, e.g. mounting specimens on microscope slides
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099Characterised by type of test elements
    • G01N2035/00138Slides
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346Heating or cooling arrangements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates

Definitions

  • the invention relates to an annealing incubator for the inverted microscopic examination of biological preparations placed in a biological preparation plate, especially in single- or multiple-well Microplate type plates.
  • the invention also relates to a procedure for manufacturing such annealing incubators and to annealing equipment containing such an incubator.
  • annealing means any temperature control (including heating, cooling, maintaining a given temperature). Nevertheless, in the case of biological preparations annealing means the setting and stable maintenance of temperatures typically higher than room temperature (maximum 52 °C), as in such fields of application generally it is not necessary to ensure temperatures lower than room temperature. At the same time, the annealing incubator and annealing equipment according to the invention also enables such applications.
  • the task to be solved with the invention is an annealing incubator and annealing equipment, which does not have the disadvantages of the state-of-the-art solutions.
  • the aim of the invention is to provide a procedure for manufacturing such incubators.
  • the set aim was solved with an annealing incubator constructed for the inverted microscopic examination of biological preparations placed in a biological preparation plate, which incubator contains a nest created for the plate, which nest is formed by a base board , a cover board and an internal wall laterally surrounding the plate, in the lower and upper range of the plate the internal wall fits to the wall of the plate with a clearance, while in the central range of the .
  • the base board contains an objective window revealing the part of the plate inside the nest containing the biological preparation
  • the cover board contains an illumination window revealing the part of the plate inside the nest containing the biological preparation
  • the incubator contains a gas-flow opening for blowing annealing gas into the central wider range of the nest.
  • the incubator contains further intermediate boards, in which there are openings forming the nest, in such a way that the internal edge of the opening of the boards forms the internal wall of the nest.
  • the incubator consists of boards placed on top of each other, in which the nest accommodating the preparation plate is formed by the openings cut into the individual boards altogether.
  • the cover board slides on the top intermediate board between a nest closing and a net opening position.
  • the illumination window is situated above the nest
  • the cover board is removed from above the nest.
  • the preparation plate can be removed from or placed inside the nest when the cover board is in the nest opening position.
  • the external wall of the incubator is constructed to suit the geometry of standard microtitre plates. Suiting the shape of the microtitre plates standardised in laboratory practice is important, because by this it is ensured that the annealing incubator can be used on any type of inverted microscopes, with a stage of any make (either manual or automatic), as generally adapters for standard microtitre plates are included in the product selection of all manufacturers.
  • the nest of the incubator is dimensioned to be able to accommodate 2-, 4- or 8-well Microplate type plates, for example explicitly suitable for the size of Lab-Tek Micro Plate types, which are the most widely used in microscope technology.
  • the invention also relates to annealing equipment using the incubator described above, which is based on that it contains a gas annealing module, the output of which is connected to the gas-flow opening of the incubator via a gas pipe.
  • the invention also relates to a procedure for manufacturing annealing incubators for the inverted microscopic examination of biological preparations placed in a biological preparation plate, which incubator contains a nest accommodating the plate.
  • the procedure is based on that:
  • the cover board is fixed onto the top intermediate board with a releasable connection enabling the opening and closing of the nest.
  • Releasable connection between the cover board ad the top intermediate board means all types of connections, which make it possible to remove the cover board from above the opening of the top intermediate board allowing by this to place the preparation plate inside the nest. Favourably during the procedure the following operations are performed:
  • a guide pin is inserted through the holes of the base board, - one of the intermediate boards is pulled onto the guide pins through its holes, and adhesive is applied on at least one of the two sides of the intermediate board and the base board facing each other,
  • Favourably plastic non-transparent black Plexiglas boards are used, and the gas-flow opening is created with the help of a milling machine after fixing the boards to each other.
  • figure 1 is the perspective view of a favourable construction of the incubator according to the invention
  • figure 2 is the top view of the base board of the incubator according to the invention.
  • figure 3 is the top view of the first intermediate board of the incubator
  • figure 4 is the top view of the second intermediate board of the incubator
  • figure 5 is the top view of the third intermediate board of the incubator
  • figure 6 is the top view of the cover board of the incubator
  • figure 7 is the top view of the stop plate of the incubator
  • figure 8 is the top view of the fastening plate of the incubator
  • figure 9 is the block diagram of the annealing equipment according to the invention.
  • FIG. 1 shows a favourable construction of the incubator 10 according to the invention.
  • the incubator 10 has a nest 12, which delimited by the base board 14 at the bottom, by the cover board 16 at the top, and by the internal wall 18 of the incubator 10 on the sides.
  • the size and geometry of the nest 12 is chosen so that from the direction of the top of the incubator 10 - in a given case after opening the cover board 16 - the biological preparation plate 20 can be placed inside it.
  • the nest 12 of the annealing incubator 10 can be dimensioned to make it suitable for accommodating single- or multiple- well Microplate (especially a 2-, 4- or 8-well Lab-Tek Microplate) type plates 20.
  • the nest 12 and the internal wall 18 of the incubator is constructed in such a way that along the lower and upper range of the inserted preparation holder 20 they rest closely against the plate 20, while along the central range of the plate 20 an appropriate gap 22 is left between the plate 20 and the internal wall 8 for allowing the flowing of the annealing gas (see figure 4).
  • the incubator 10 is constructed for inverted microscopic examinations, the microscope objective is situated below the stage, while the object is illuminated from above.
  • the base board 14 and cover board 16 of the incubator 10 there is an objective window 24 and an illumination window 26.
  • the objective window 24 in the base board 14 reveals the part of the plate 20 inserted in the nest 12 containing the biological preparation, and so the biological preparation can be studied from below through the microscope objective.
  • the illumination window in the cover board 16 makes it possible to transilluminate the biological preparation in the inverted microscope from above with a light beam (e.g. laser beam).
  • the biological preparation is annealed by entering heated (or cooled) gas, e.g. air.
  • the incubator 10 has a gas-flow opening 28 leading into the central wider range of the nest 12, that is into the gap 22 between the inserted plate 20 and the internal wall 18 of the incubator 10.
  • Figures 2-8 show the building units of a construction example constructed from parallel boards 30 placed on top of each other: the base board 14 is at the bottom (figure 2), it is followed by a first, second and third intermediate board 32 (figure 3, 4 and 5), and finally by the cover board 16 (figure 6). There is also a stop plate 34 situated in the same plane as the cover board 16 (figure 7), and the fastening plate 36 is attached to it from above (figure 8).
  • the boards 30 are made of a few mm thick, non- transparent (to prevent the light illuminating the preparation from advancing in it), black (to reduce harmful light reflections) Plexiglas boards, and favourably they are attached to each other with an adhesive.
  • the base board 14 is thinner (e.g. 1-1.5 mm thick), while the other boards 30 are thicker (about 4-6 mm thick, e.g. 5 mm thick).
  • the boards 30 are non-transparent, so the sample, that is the biological preparation is illuminated exclusively by the light beam arriving through the window 26 of the cover board 16.
  • the base board 14 is made of a thin (e.g. 1-1.5 mm thick) Plexiglas board so that the examined biological preparation is not lifted from the microscope stage too much, which would make it difficult, or practically impossible above a certain distance, to focus on the sample. For this reason the base board 14 can even be significantly thinner than 1 mm.
  • the window 24 can be left empty. However, it is also possible to cover the window 24 with glass or with some other plate less disturbing from an optical aspect, in order to protect the sensitive microscope objective from the heated gas flowing in the gap 22.
  • the internal wall 18 of the incubator 10, and also the nest 12, is formed by the internal edges 40 of the openings 38 of the intermediate boards 32. It can be seen that the intermediate boards 32 have openings 38 of different sizes.
  • the preparation plate 20 fits with a clearance into the opening 38 of the first intermediate board 32 neighbouring the base board 14, while there is a significant gap 22 left between the internal edges 40 of the opening 38 of the second intermediate board 32 falling in the central range of the plate 20 and the plate 20, in which gap 22 the annealing gas can flow freely, ensuring by this appropriate annealing along the entire periphery of the plate 20.
  • the opening 38 of the third intermediate board 32 accommodates the plate 20 inserted in the nest 12 with a clearance again, so the annealing gas cannot leave the gap 22 too fast, which increases the efficiency of annealing. It is also possible to cut the openings 38 in the first and third intermediate boards 32 diagonally, rather than with a perpendicular edge, as a result of which the cross- section of the opening 38 gradually narrower towards the bottom of the first intermediate board 32 and the top of the third intermediate board 32. It may be favourable to make indents in the edge 40 of the opening 38 of the third intermediate board 32, on two sides, to make it easier to grab the preparation plate 20 inserted in the nest 12 and lift it out with one's fingers or using a tool.
  • the intermediate boards 32 are 4-6 mm thick, and favourable they are cut out from non-transparent Plexiglas boards.
  • the external geometry of the boards 30 is chosen to follow the shape of the microtitre plates regarded as standard in laboratory practice.
  • the incubator 10 can be used with any type of inverted microscope, with a stage of any make (manual or automatic), as generally adapters for standard microtitre plates are included in the product selection of all manufacturers.
  • Such adapters assume that a corner on the right or on the left is cut off, so favourably - to make the incubator 10 compatible both with right-side and left-side adapters - cut corners 42 are created on the boards 30 at the necessary height both on the right side and on the left side.
  • the cover board 16 is attached to the third intermediate board 32 via a releasable connection,
  • the releasable connection is realised with the help of the stop plate 34 and the fastening plate 36.
  • the stop plate 34 attached to the third intermediate board functions as a distance piece for the fastening plate 36, the arresting range 44 of which extends over the stop plate 34.
  • the stop plate 34 keeps the fastening plate 36 away from the third intermediate board 32 exactly at a distance that makes it possible to slide in the cover board 16 between the arresting range 44 of the fastening plate 36 and the third intermediate board 32, where it is arrested by the fastening plate 36 without any clearance, ensuring by this the releasable connection, for the releasing of which the cover board 16 is pulled out from below the fastening plate 36 from the same direction.
  • the sides of the cover board 16 and the stop plate 34 contacting each other are constructed in a complementary way, so that when the cover board 16 is pushed below the arresting range 44 of the fastening plate 36 until it hits the stop plate 34, favourably the cover board 16 cannot move laterally either.
  • extension pieces 46 extending from the cover board 16 towards the stop plate 34, closely fitting the opposing periphery of the stop plate 34 created in a complementary way.
  • extension pieces 46 extending from the cover board 16 towards the stop plate 34, closely fitting the opposing periphery of the stop plate 34 created in a complementary way.
  • the releasable connection between the cover board 16 and the third (top) intermediate board 32 can mean all connections, which make it possible to remove the cover board 16 from above the opening 38 of the third (top) intermediate board 32 in order to free the opening 38 and place the preparation plate 20 in the nest 12 freely.
  • the cover board 16 can also be attached to the third intermediate board 32 (or to any lower intermediate board 32) with a pivoted joint, releasing the connection simply means that the cover board 16 is folded out around the picot, in the course of which the cover board 16 is lifted away from the upper surface of the third intermediate board 32 leaving the opening 38 of the intermediate plate 32, that is the entrance to the nest 12 free.
  • Completely different types of connections such as form-locking complementary, clicking, magnetic, etc.
  • connections are also possible, which are suitable for releasing the cover board 16 from the intermediate board 32 at least to an extent to make its opening 38 free so as to insert the plate 20. Obviously it is necessary, if the cross-section of the window 26 of the cover board 16 is smaller than the plate 20.
  • the window 26 of the cover board 16 is dimensioned to enable the transillumination of the biological preparation during the inverted microscopic examination on the one part, and on the other part to allow the annealing gas entered into the central wider range of the nest 12 to leave.
  • the latter takes place automatically, if the plate 20 is a commonly used 2-, 4- or 8-well Microplate, in which case the annealing gas reaches the window 26 through the gaps between the individual wells of the plate 20 too.
  • the window 26 can be covered with a light-permeable plate (for example glass plate) to protect the biological preparation from slight contaminations coming from above. In this case a gas outlet opening can be created somewhere else.
  • the cover board 16 locks the plate 20 inside the nest 12, so favourably the cross-section of the window 26 is smaller than the cross-section of the plate 20, so the plate 20 can be removed from the nest 12 only after releasing the releasable connection of the cover board 6 and removing the cover board 6 at least partially.
  • the annealing incubator 10 is assembled as described below, using the boards 30 shown in figures 2- 8. It can be seen that there are holes 48 drilled into the corners of the base board 14, the three intermediate boards 32, and into the corners of the stop plate 34 and the fastening plate 36 on the one side. A guide pin is placed in each one of the four holes 48 drilled into the base board, then the intermediate boards 32 are stringed onto these guide pins one after the other. Favourably adhesive should be applied on at least one of the contacting surfaces of the boards 30. After putting the individual boards 30 in their place, the boards are pressed together to allow the adhesive to spread appropriately.
  • the stop plate 34 and the fastening plate 36 are also stringed onto the guide pins on the appropriate side.
  • the two holes and guide pins on the stop plate 34 are also enough for performing the above operation, as precise gluing can be realised after fixing the given planes in two points.
  • the stringed and glued boards 30 are kept pressed together for a certain period, for example in the case of Plexiglas boards and Plexiglas glue they are gripped in a vice for about an hour to allow the glue to diffuse into the Plexiglas boards 30, in order to obtain a massive block enclosed on the sides.
  • the guide pins can be removed.
  • favourably threaded spindles (headless threaded rods) of a length equivalent to the height of the block to be created are used as guide pins, the ends of which at the top and at the bottom exactly coincide with the external plane of the cover board 16 and the base board 14, and these metal pins are left in the holes 48.
  • bolts can be used as guide. pins, and by placing nuts on the protruding end of the bolts, with the help of the nuts the boards 30 can be fastened together even more tightly.
  • the cover board 16 is fixed to the intermediate board 32 on top with a releasable connection, for example in the present case by sliding the cover board 6 below the fastening plate 36.
  • the boards 30 shown in figures 2-8 do have a lateral opening for entering gas.
  • the reason for this is that favourably the gas-flow opening 28 of the incubator 10 is created subsequently, after the individual boards 30 have been attached to each other.
  • the gas-flow opening 28 can be created favourably with the help of a milling machine in one of the side walls of the Plexiglas block created by gluing, so that the opening 28 leads into the central wider range of the nest 12.
  • the opening 28 is created before fitting the cover board 16 in its place, and favourably on the side of the incubator 10 where the stop plate 34 and the fastening plate 36 are fixed permanently.
  • the place of the gas-flow opening 28 to be created and the direction of the gas flow is marked with arrow A in figures 2-8.
  • Figure 10 shows the block diagram of annealing equipment 50 containing an annealing incubator 10 according to the invention.
  • the gas-glow opening 28 of the incubator 10 is connected to a gas annealing (heating or - in a given case - cooling) module 52 via a gas pipe 54.
  • the gas annealing module 52 heats (or in a given case cools) the input gas, for example air, which then flows through the gas pipe 54 and the gas-flow opening 28 of the incubator 10 and enters the nest 12, in the wider central range of which (practically in the gap 22) it flows around the plate 20 and anneals (heats or, in a given case, cools) the biological preparation stored therein.
  • the gas annealing module 52 can be, for example, a heat exchanger, which has, for example, built-in active current generator heating elements.
  • the temperature of the latter is controlled with temperature controller 55, for example a Physiological-Biological Temperature Controller distributed by Supertech Instruments.
  • One or more heat sensing devices 56 are used for controlling the temperature.
  • a high-precision Dallas Semiconductor DS18S20 thermometer can be placed in the gas annealing (heat exchanger) module 52.
  • the gas temperature inside the heat exchanger module 52 can be different from the temperature reached in the nest 12 of the incubator 10 by as much as about 10 °C, because some heat loss occurs inevitably along the gas pipe 54.
  • thermometer 56 can be placed in the incubator 10, for example at the point in the internal wall 18 of the nest 12 opposite the gas-flow opening 28, so it measures directly the temperature inside the nest 12.
  • a thermometer 56 can be placed at the edge 40 of the opening 38 of the second intermediate board 32 opposite the gas-flow opening 28 , the signal transmitting cable of which can be taken to the temperature controller 55 through the hole 58 leading into the internal edge 40 of the intermediate board 32.
  • a suitable thermometer 56 can be for example a precision temperature measuring circuit operating with a Pt100 sensor, which Pt100 sensor is arranged in or at the internal wall 18 of the nest 12.
  • the gas pipe 54 connecting the module 52 with the gas-flow opening 28 of the incubator 10 is a flexible, heat-insulated, plastic pipe.
  • the heat exchanger module 52 can be placed relatively close to the incubator 10, for example on an adjustable stand extending over the microscope, and by this the length of the gas pipe 54 can be minimised, and so the intermediate heat loss can be reduced.
  • Filtered air of room temperature and a constant volume flow rate produced by a gas source 60 for example by an Air Flow Heater Controller manufactured by Supertech Instruments, is taken to the input of the heat exchanger module 52 favourably through a flexible plastic pipe.
  • a gas source 60 for example by an Air Flow Heater Controller manufactured by Supertech Instruments.
  • the volume flow rate it must be taken into consideration that if the volume flow rate is too low, it will not result in satisfactory annealing, while if the volume flow rate is too high, it may result in the mechanical vibration of the preparation during measuring.
  • air output of a volume flow rate of about 2-20 l/minute is suitable for annealing the biological preparation placed in the nest 12 of the incubator 10.
  • the Air Flow Heater Controller serving as a gas source 60 contains a thermometer 56, for example a precision temperature measuring circuit operating with a Pt100 sensor.
  • the Pt 00 sensor is situated inside the incubator 10, on the side of the nest 12 opposite the incoming air flow, as it is described above in connection with the temperature controller 55.
  • the temperature controller e.g. Physiological-Biological Temperature Controller
  • the actual current temperature of the inside of the annealing vessel is always displayed on the LSD screen placed on the front of the Air Flow Heater Controller serving as a gas source 60.
  • the incubator 10 constructed from boards 30 described here is only an example, the main advantages of the invention are represented by the nest 12 suitable for annealing constructed for the preparation plate 20 and its special geometry, which can be realised differently too, as it is obvious for a person skilled in the art, for example an incubator 10 of a similar shape as the above can be made by injection-moulding, in the course of which two separate elements - the block accommodating the nest 12 and the cover board 6 attached to it with a releasable connection - are manufactured.
  • Plexiglas boards 30 also serves as an example, they are advantageous first of all because they are cost efficient and favourable workable.
  • the base board 14 can also be made of a different heat insulating material, for example Bakelite, in the case of which the desired thinness of even less than 1 mm is easier to ensure.

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un incubateur à régulation de température (10) conçu pour l'examen microscopique inversé de préparations biologiques placées dans une plaque (20) pour préparations biologiques. L'invention est caractérisée en ce qu'elle comporte une cavité (12) formée dans la plaque, laquelle cavité (12) étant constituée d'une plaque de base (14), d'une plaque de recouvrement (16) et d'une paroi interne (18) entourant latéralement la plaque (20), et en ce que, dans les parties inférieure et supérieure de la plaque (20), la paroi interne (18) s'adapte à la paroi de la plaque (20) avec un jeu, tandis que dans la partie centrale de la plaque (20) elle s'élargit, formant un espace (22) par rapport à la plaque (20), ce qui permet un écoulement de gaz autour de la plaque (20). La plaque de base (14) présente une fenêtre pour objectif (24) révélant la partie de la plaque (20) située à l'intérieur de la cavité (12) qui contient la préparation biologique, tandis que la plaque de recouvrement (16) présente une fenêtre d'éclairage (26) révélant la partie de la plaque (20) située à l'intérieur de la cavité (12) contenant la préparation biologique, et l'incubateur (10) présente une ouverture d'écoulement de gaz (28) permettant d'insuffler le gaz de régulation de température dans la partie centrale la plus large de la cavité (12). L'invention concerne également un équipement de régulation de température (50) qui contient ledit incubateur (10), et un procédé de fabrication de tels incubateurs (10).
PCT/HU2010/000111 2009-10-30 2010-10-25 Incubateur à régulation de température WO2011051738A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU0900681A HUP0900681A2 (en) 2009-10-30 2009-10-30 Tempering incubator
HUP0900681 2009-10-30

Publications (1)

Publication Number Publication Date
WO2011051738A1 true WO2011051738A1 (fr) 2011-05-05

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WO (1) WO2011051738A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage
US5552321A (en) * 1993-08-24 1996-09-03 Bioptechs Inc. Temperature controlled culture dish apparatus
JP2003107364A (ja) * 2001-09-27 2003-04-09 Tokai Hit:Kk 顕微鏡観察用培養器
EP1548484A1 (fr) * 2002-08-28 2005-06-29 Tokai Hit Co. Ltd. Incubateur pour l'observation microscopique
EP1936425A1 (fr) * 2005-10-13 2008-06-25 Tokai Hit Co. Ltd. Platine porte-objet et corps de microscope

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage
US5552321A (en) * 1993-08-24 1996-09-03 Bioptechs Inc. Temperature controlled culture dish apparatus
JP2003107364A (ja) * 2001-09-27 2003-04-09 Tokai Hit:Kk 顕微鏡観察用培養器
EP1548484A1 (fr) * 2002-08-28 2005-06-29 Tokai Hit Co. Ltd. Incubateur pour l'observation microscopique
EP1936425A1 (fr) * 2005-10-13 2008-06-25 Tokai Hit Co. Ltd. Platine porte-objet et corps de microscope

Also Published As

Publication number Publication date
HU0900681D0 (en) 2010-01-28
HUP0900681A2 (en) 2011-10-28

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