WO2011048806A1 - Agent for improving sperm motility, agent for improving fertility, method for improving sperm motility and method for improving fertility - Google Patents

Agent for improving sperm motility, agent for improving fertility, method for improving sperm motility and method for improving fertility Download PDF

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WO2011048806A1
WO2011048806A1 PCT/JP2010/006209 JP2010006209W WO2011048806A1 WO 2011048806 A1 WO2011048806 A1 WO 2011048806A1 JP 2010006209 W JP2010006209 W JP 2010006209W WO 2011048806 A1 WO2011048806 A1 WO 2011048806A1
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improving
linolenic acid
gamma
sperm
fertility
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French (fr)
Japanese (ja)
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中島寿昭
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出光興産株式会社
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
    • A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis

Definitions

  • the present invention relates to a technique for improving the reproduction performance of livestock and the like.
  • Improving breeding performance is an important issue in the livestock industry. Even if the conception rate of livestock is different by 1%, the effect is extremely large. For example, a large England sow is conceived about twice a year and gives birth to about 10 children in a single conception. In the case of a farm that raises 1000 mother pigs, if the conception rate is different by 10%, the number of individuals after one year will differ by several thousand. Therefore, improving the conception rate as much as possible has great significance in the livestock industry. Improving the labor turnover rate related to the number of conceptions per year is equally significant in the livestock industry.
  • Non-Patent Document 1 describes that gamma-linolenic acid is given to sows to improve the ovulation rate and increase normal embryos.
  • Patent Document 1 describes that the number of pups, the average number of deliveries per year, and the like are increased by feeding a sow with a feed containing gamma-linolenic acid or the like.
  • Patent Document 2 discloses an animal feed containing gamma-linolenic acid by heat-treating filamentous fungi containing gamma-linolenic acid and the like, thereby improving the oxidation stability and thermal stability of gamma-linolenic acid. Is provided. In addition, it is described that feeding this animal with animals has health improvement effects such as a decrease in liver dysfunction, an increase in body weight, an improvement in meat quality, and a decrease in mortality. Patent Document 3 describes that by giving a cow a feed containing gamma-linolenic acid or the like, effects such as improvement in conception rate and shortening of the number of days between labor can be obtained.
  • the present inventor has focused on the existence of prostaglandin as a therapeutic agent for ED (Erectile Dysfunction). Since gamma-linolenic acid is a precursor of this prostaglandin, it has been thought that gamma-linolenic acid may contribute not only to females but also to male reproductive performance. Thus, through extensive research, it was found that gamma-linolenic acid can improve sperm vitality. In addition, it has been found that seeding females using such activated sperm improves the conception rate and the labor turnover rate of females. Furthermore, it has been found that by giving gamma-linolenic acid to both males and females, the conception rate and labor turnover rate of females can be improved.
  • the present invention contains a gamma-linolenic acid-containing sperm vitality-improving agent, gamma-linolenic acid, and a fertilization rate that can improve the conception rate of females seeded with male sperm by feeding them to males. It aims at providing an improving agent, a sperm vitality improvement method, and a conception rate improvement method.
  • the sperm vitality improver of the present invention contains gamma-linolenic acid.
  • the fertility improving agent of the present invention contains gamma-linolenic acid and is fed to males to improve the fertility of females seeded with male sperm.
  • the method for improving sperm vitality of the present invention is a method for improving sperm vitality by feeding gamma-linolenic acid to males of domestic animals.
  • the method for improving the conception rate of the present invention is a method in which gamma-linolenic acid is fed to a male male and the male sperm is seeded on a female female to improve the conception rate of the female.
  • the present invention it is possible to improve the sperm vitality of male animals such as livestock, thereby improving the conception rate and the labor turnover rate of females.
  • Example 1 and Comparative Example 1 It is a figure which shows the experimental condition of Example 1 and Comparative Example 1, and its result. It is the figure which compared the experimental result of Example 1 and Comparative Example 1.
  • FIG. It is a figure which shows the experimental condition of Example 2 and Comparative Example 2, and its result. It is a figure which shows the experimental conditions of Example 3, Reference Example 1, and Comparative Example 3, and its result.
  • a sperm vitality improving agent a conception rate improving agent, a sperm vitality improving method, and a conception rate improving method according to an embodiment of the present invention will be specifically described.
  • the sperm vitality improving agent of the present embodiment contains gamma-linolenic acid.
  • the sperm vitality improving method of this embodiment is to supply gamma-linolenic acid to male animals such as livestock to improve the sperm vitality.
  • “Sperm vitality” means the sperm vitality in a narrow sense obtained by grading the sperm activity state confirmed by microscopic observation, the sperm viability, and the viable sperm concentration.
  • the sperm vitality in the narrow sense is determined in three stages in the following examples, “3" is the most active forward movement, “2" is the active movement, “1” is the weak movement It is supposed to be.
  • Sperm survival is the ratio of the number of sperm moving to the total number of sperm. Generally, if you own one excellent breeding boar, it is possible to breed more than 100 sows for breeding. Therefore, the production of male individuals having sperm vitality and excellent reproduction ability greatly contributes to the development of the livestock industry.
  • GLA Gamma-linolenic acid
  • ⁇ -linolenic acid is one of triunsaturated fatty acids having 18 carbon atoms. All of the C6, C9, and C12 positions have a cis-type double bond. It is classified as an omega-6 fatty acid ( ⁇ 6 fatty acid, 18: 3, n-6).
  • Gamma-linolenic acid becomes dihomo- ⁇ -linolenic acid (abbreviation DGLA) in the animal body and becomes a raw material for arachidonic acid (abbreviation AA) and prostaglandin (abbreviation PG).
  • the source of gamma-linolenic acid is not particularly limited.
  • fungi such as Mucor genus, Mortierella genus, and Rizopus genus
  • plants such as evening primrose, borage, black currant, hemp, algae such as spirulina, and animals such as emu Can be obtained from the oils and fats.
  • gamma-linolenic acid can be obtained by chemical synthesis, and commercially available products may be used.
  • gamma-linolenic acid becomes dihomo- ⁇ -linolenic acid in the animal body and becomes a raw material such as arachidonic acid and prostaglandin. Therefore, these omega-6 fatty acids are used in this embodiment. Even if it is used in place of gamma-linolenic acid, it is presumed that an effect close to that obtained when gamma-linolenic acid is used can be obtained.
  • esters obtained by reaction with various alcohols such as ethyl esters, glycerol esters, phospholipids, or inorganic or organic bases
  • examples thereof include salts obtained by acting in a ratio, such as sodium salt and potassium salt.
  • the sperm vitality improving effect by the sperm vitality improving agent of the present embodiment is obtained based on the contained gamma-linolenic acid, from the viewpoint of the effect, the gamma-linolenic acid content ratio in the sperm vitality improving agent is Higher is preferable.
  • various amino acids may be contained in addition to gamma-linolenic acid.
  • arginine, lysine, threonine, methionine, L-valine, tryptophan and the like are preferably contained together with gamma-linolenic acid.
  • arginine is abundant in semen and is said to improve sperm vitality.
  • sperm vitality improving agent it is also preferable to contain astaxanthin, vitamin E, coenzyme Q10, and carnitine in the sperm vitality improving agent of this embodiment. This is because both are components related to energy production and may contribute to improving sperm vitality. Moreover, it is also preferable to contain mineral components, such as zinc and selenium, in the sperm vitality improvement agent of this embodiment.
  • a subject to which the sperm vitality improving agent of the present embodiment is fed is a male animal such as livestock.
  • Livestock refers to mammals and birds other than humans, such as dogs, cats, pigs, cows, horses, sheep, and chickens, and is used as those raised by humans. Moreover, even if administered to humans, it is considered that there is an effect of improving sperm vitality.
  • the sperm vitality-improving agent of the present embodiment may be blended with feed and fed separately from the feed. Moreover, it may be fed together with other compounding ingredients, and the dosage form is not particularly limited.
  • GLA oil When GLA oil is used, it can be mixed with feed or fed in soft gelatin capsules.
  • the feeding period of the sperm vitality improving agent of this embodiment is preferably at least 3 days before sperm collection. This is because if the period is shorter than this, sperm vitality may not be sufficiently improved.
  • the dosage of the sperm vitality improving agent of this embodiment is preferably such that the amount of gamma-linolenic acid per day is 0.05 mg / kg to 100 mg / kg of the body weight of the livestock to be administered. Further, it is more preferably 1 mg / kg to 100 mg / kg, further preferably 1 mg / kg to 50 mg / kg, still more preferably 3 mg / kg to 30 mg / kg.
  • 0.015 g to 30 g can be suitably administered every morning, more preferably 0.3 g to 30 g, still more preferably 0.3 g to 15 g, It is even more preferable to use 0.9 g to 9 g.
  • Patent Documents 1 and 3 a feed containing gamma-linolenic acid or the like is fed to each of sows and cows in an amount of 20 to 200 g per head per day in terms of the fatty acid. .
  • the sperm vitality improving agent of the present embodiment sufficient effects can be obtained by feeding 0.3 g to 30 g, for example, every morning as described above.
  • the fertility-enhancing agent of the present embodiment contains gamma-linolenic acid, and is fed to male animals such as livestock to improve the fertility rate of females seeded with male sperm. Moreover, as a fertility improver of this embodiment, it is fed to both males and females of animals such as livestock to improve male sperm vitality and improve the fertility rate of females seeded with the male sperm It is also preferable to make it.
  • the method of improving the fertility rate of the present embodiment is to feed gamma-linolenic acid to a male animal such as a domestic animal, seed the male sperm to a female, and improve the fertility rate of the female.
  • gamma-linolenic acid is fed to male animals such as livestock, the sperm vitality of males is improved, and the female females such as domestic animals fed gamma-linolenic acid are treated with gamma-linolenic acid. It is also preferred to seed male sperm to improve the female fertility.
  • “Conception rate” means the ratio of the number of females with conception to the number of females with seeding.
  • omega-6 fatty acids may be used in place of gamma-linolenic acid, or these derivatives may be used in place of gamma-linolenic acid, which contains amino acids such as arginine and other compounding ingredients such as astaxanthin. May be.
  • the fertility improvement agent of this embodiment can be fed to male animals such as livestock. That is, the female fertility rate is improved by seeding a female not fed with a conception rate improving agent using male sperm fed with a fertility rate improving agent. This is thought to be due to the activation of male sperm fed with a fertility enhancer.
  • the fertility improving agent of this embodiment is also preferable to feed the fertility improving agent of this embodiment to both males and females of animals such as livestock.
  • the fertility improving agent containing gamma-linolenic acid improves the conception rate of the female itself, and the fertility improving agent activates the male sperm. It becomes possible to greatly improve.
  • the fertility improvement agent of this embodiment is effective also when administered with respect to a human.
  • the method of feeding the fertility improving agent of this embodiment can be the same as the method of feeding the sperm vitality improving agent, and the feeding period can be the same. Moreover, when paying
  • the amount of the fertility improver of this embodiment is 0.05 mg / kg to 100 mg / kg of the weight of the livestock to be fed, as in the case of the sperm vitality improver. It is preferable to make it kg. Further, it is more preferably 1 mg / kg to 100 mg / kg, further preferably 1 mg / kg to 50 mg / kg, still more preferably 3 mg / kg to 30 mg / kg.
  • 0.01 g to 20 g can be suitably administered every morning, more preferably 0.2 g to 20 g, still more preferably 0.2 g to 10 g, It is even more preferable that the amount be 0.6 g to 6 g.
  • Example 1 Four Duloc breeds (D1: body weight 300 kg, D2: body weight 320 kg, D3: body weight 280 kg, D4: body weight 300 kg), and a large Yorkshire species 1 bred in the Marni Mark-containing feed martel (Kashima Feed Co., Ltd.) A sperm vitality improvement test containing gamma-linolenic acid of the present invention was given to the head (W1: body weight 300 kg), and a sperm vitality improvement test was conducted.
  • Mucor silcineroides cells were measured with a measuring cup once a morning for each pig and fed in a top dress.
  • Mucor silcineroides cells contain gamma-linolenic acid-containing oil and fat, and the content thereof is about 75 mg as gamma-linolenic acid in 1 g of cells. Therefore, about 40 g of cells correspond to about 3 g of gamma-linolenic acid, and the amount of gamma-linolenic acid fed to 1 kg of body weight of each boar is about 10 mg.
  • the salary period varies depending on the number of samples to be collected, and becomes longer depending on the number. For example, at Deyulock D1, six times of sampling (6 samples) are performed, and the first time of sampling is the fifth day after the start of salary, the second time is the 19th day, and the third time is the 33rd day. The last collection was 75 days after the start of salary, and the salary was paid for a total of 75 days.
  • each pig was placed on a pseudo-female about once every two weeks and semen was collected. After collection, a semen test was performed. Semen examination was performed by microscopic examination. The above results are shown in FIG.
  • the number of samples indicates the number of containers from which semen was collected, and corresponds to the number of collections. Further, the collected semen was diluted with a storage solution so that about 5.5 billion spermatozoa were contained per bottle, and a semen bottle was prepared.
  • the average sperm vitality is the average value of the graded sperm activity, “3” for the most active forward movement, “2” for the active movement, and weak movement. It is calculated as “1”.
  • Average survival is the average percentage of moving sperm in each sample. The survival rate was obtained by measuring the number of moving spermatozoa using a hemocytometer and calculating the ratio to the total sperm count. The average viable sperm concentration refers to the average viable sperm number per 1 ml of collected semen.
  • Example 1 Comparative Example 1
  • a sperm vitality improver containing gamma-linolenic acid was fed before performing Example 1, and a semen test was conducted. Sperm vitality was confirmed. The result is shown in FIG.
  • FIG. 2 shows the average sperm vitality, average survival rate, average survival sperm concentration, and concentration increase rate for each identical boar in Example 1 and Comparative Example 1.
  • the average sperm vitality of Comparative Examples 1-1 and 1-2 was 2.8, showing relatively high values. In both cases of -1 and 1-2, the sperm vitality is further improved to 3.0.
  • the average sperm vitality of Comparative Examples 1-3, 1-4, and 1-5 were both 2.3, indicating relatively low values. 1-4 was 2.8, and Examples 1-3 and 1-5 were 3.0, which significantly improved sperm vitality.
  • the average survival rate also shows an increase of 3 to 6% when comparing the examples and comparative examples for each individual. Furthermore, although the average viable sperm concentration varies greatly among individuals, the results of the examples are all increased by about 10% to several tens of% with respect to the comparative examples. In particular, the average viable sperm concentration is significantly increased in Duroc 1 and 4 and Large Yorkshire.
  • Confirmation test of GLA feeding effect on boar (Example 2) About 40 g of mucor silcineroides cells were measured with a measuring cup once a morning for Duroc D5 (body weight 300 kg) of a breeding boar bred on Marni-mark mixed feed Martell and fed in a top dress.
  • the amount of gamma-linolenic acid was about 3 g as in Example 1
  • the amount of gamma-linolenic acid per kg body weight of Duroc D5 was about 10 mg.
  • Example 2 The same boar Duroc D5 as in Example 2 was raised on the Marni-marked mixed feed martel under the same conditions as in Example 2 except that gamma-linolenic acid was not fed before Example 2. Then, a semen test was performed to confirm the sperm vitality. As a result, the sperm vitality was 2, and the survival rate was 80%.
  • 20 large sows of the large Yorkshire (paternal) -Landlace (maternal) breed were seeded. These sows were bred in the same manner as in Example 2 using marni-marked mixed feed martel that does not contain gamma-linolenic acid. As a result, as shown in FIG. 3, the number of conception heads was 18, and the conception rate was 90%.
  • Example 3 Confirmation test of GLA feeding effect on boar and sow (Example 3)
  • the semen collected in Example 1 was used for land races of 100-200 kg sows with a weight of 180-200 kg, which were bred in the Marni Mark mixed feed Martell-100 large Yorkshire species and 103 large Yorkshire-Landlace species. Used for seeding. These sows were fed with gamma-linolenic acid in the feed. Specifically, the feed amount of feed was 4 kg / day for 7 days before parturition, and the same mucor silcineroides cells as in Example 1 were blended in the feed of each sow to 0.2 wt%.
  • the amount of the microbial cells is 8 g, and about 75 mg of gamma-linolenic acid is contained in 1 g of the microbial cells. Therefore, 8 g of microbial cells corresponds to about 600 mg as the amount of gamma-linolenic acid.
  • the amount of gamma-linolenic acid fed per kg body weight is about 3 mg.
  • the feed amount of feed from parturition to 7 days after weaning was 6 kg / day, and Mucor silcineroides cells were blended in the feed of each sow to 0.08 wt%.
  • Example 2 seeding was performed by this crossing and artificial fertilization as in Example 2.
  • semen bottles obtained from the Duroc breed (D1, D2, D3, D4) boar of Example 1 were used for artificial fertilization.
  • FIG. 4 96 out of 100 Landrace-Large Yorkshire species were conceived, 100 out of 103 large Yorkshire-Landrace species were conceived, and the total number of conceived heads was 196.
  • the rate was 97%. 97% is considered to be the highest conception rate because there are some sows that contain individual defects such as infertility among the test pigs.
  • the average labor turnover rate in Example 3 was 2.46.
  • Example 3 seeding was performed by this crossing and artificial fertilization as in Example 3.
  • a semen bottle obtained from the Duroc (D1) boar of Comparative Example 1 was used for artificial fertilization.
  • FIG. 4 40 out of 44 Landrace-Largeshire species were conceived, 38 out of 40 large Yorkshire-Landrace species were conceived, and the total number of conceived heads was 78. The rate was 93%.
  • the average labor turnover rate in Reference Example 1 was 2.36.
  • Example 3 when the sows fed gamma-linolenic acid were seeded with sperm from boars fed gamma-linolenic acid, the conception rate was 97%, Compared to the result of Comparative Example 3, the improvement is 12%.
  • the average labor turnover rate is 2.15 in Comparative Example 3, 2.36 in Reference Example 1, and 2.46 in Example 3.
  • the present invention is not limited to the above-described embodiments and examples, and it goes without saying that various modifications can be made within the scope of the present invention.
  • pigs are used, but the same can be applied to livestock such as cows, horses, and birds.
  • an effect on humans can be expected.
  • the present invention can be suitably used for efficiently breeding animals such as livestock.

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Abstract

Provided are an agent for improving sperm motility which comprises gamma-linolenic acid, and an agent for improving fertility which comprises gamma-linolenic acid. When the agent for improving fertility is administered to a male and a female is inseminated with the semen from the male, the fertility of the female can be improved. It is also possible to administer the agent for improving fertility to both a male and a female. Thus, the sperm motility of the male can be improved and the fertility of the female inseminated with the semen from the male can be improved.

Description

精子活力向上剤、受胎率向上剤、精子活力向上方法、及び受胎率向上方法Sperm vitality improving agent, conception rate improving agent, sperm vitality improving method, and conception rate improving method
　本発明は、家畜などの繁殖成績を向上させる技術に関する。 The present invention relates to a technique for improving the reproduction performance of livestock and the like.
　畜産業などにおいて、繁殖成績の向上は重要な課題である。家畜の受胎率が１％違っただけでもその影響は極めて大きい。例えば、大ヨークシャー種の雌豚は、１年に２回ほど受胎し、１回の受胎で１０頭程度の子供を産む。１０００頭の母豚を飼育する農場の場合、仮に受胎率が１０％違えば、１年後の個体数には数千頭の違いが生じることになる。
　したがって、受胎率を少しでも向上させることは、畜産業において大きな意味がある。また、１年間の受胎回数に関係する分娩回転率を向上させることも、畜産業においては同様に大きな意味がある。 Improving breeding performance is an important issue in the livestock industry. Even if the conception rate of livestock is different by 1%, the effect is extremely large. For example, a large Yorkshire sow is conceived about twice a year and gives birth to about 10 children in a single conception. In the case of a farm that raises 1000 mother pigs, if the conception rate is different by 10%, the number of individuals after one year will differ by several thousand.
Therefore, improving the conception rate as much as possible has great significance in the livestock industry. Improving the labor turnover rate related to the number of conceptions per year is equally significant in the livestock industry.
　従来、このような家畜の繁殖成績を向上させるための様々な技術が提案されている。
　例えば、非特許文献１には、雌豚にガンマ－リノレン酸を与えることで、排卵率が改善し、正常胚が増加することが記載されている。
　また、特許文献１には、ガンマ－リノレン酸などを含有する飼料を雌豚に与えることで、産子数、年間平均分娩回数等を増加させることが記載されている。 Conventionally, various techniques for improving the reproduction performance of such livestock have been proposed.
For example, Non-Patent Document 1 describes that gamma-linolenic acid is given to sows to improve the ovulation rate and increase normal embryos.
Patent Document 1 describes that the number of pups, the average number of deliveries per year, and the like are increased by feeding a sow with a feed containing gamma-linolenic acid or the like.
　特許文献２には、ガンマ－リノレン酸等を含有する糸状菌を熱処理することで、ガンマ－リノレン酸の酸化安定性、及び熱安定性を向上させ、ガンマ－リノレン酸を含有させた動物用飼料を提供することが記載されている。また、この飼料を動物に与えることで、肝機能障害の減少、体重増加、肉質改善、へい死率低下などの健康改善効果があることが記載されている。
　特許文献３には、雌牛にガンマ－リノレン酸などを含有する飼料を与えることで、受胎率の向上、分娩間隔日数の短縮などの効果が得られることが記載されている。 Patent Document 2 discloses an animal feed containing gamma-linolenic acid by heat-treating filamentous fungi containing gamma-linolenic acid and the like, thereby improving the oxidation stability and thermal stability of gamma-linolenic acid. Is provided. In addition, it is described that feeding this animal with animals has health improvement effects such as a decrease in liver dysfunction, an increase in body weight, an improvement in meat quality, and a decrease in mortality.
Patent Document 3 describes that by giving a cow a feed containing gamma-linolenic acid or the like, effects such as improvement in conception rate and shortening of the number of days between labor can be obtained.
特許第３４９２３４９号公報Japanese Patent No. 3492349 特公平３－２７１８４号公報Japanese Patent Publication No. 3-27184 特開２００８－１２５３７７号公報JP 2008-125377 A
　しかしながら、このような従来の繁殖成績の向上に関する技術は、雌に対して適用されるのみであり、雄に対してはこれまで全く考慮されてこなかった。
　これは、雌の母体の機能を向上させることは、繁殖に直接的な影響を与えることが想像できる一方、雄の精子の機能向上を図ることは、容易には考えられないことであったためではないかと思われる。 However, such conventional techniques for improving reproductive performance are only applied to females and have not been considered at all for males.
It can be imagined that improving the function of the female maternal body has a direct effect on reproduction, but it is not easy to think about improving the function of the male sperm. It seems that there is not.
　これに対し、本発明者は、ＥＤ（Erectile Dysfunction）の治療薬として、プロスタグランジンンが存在していることに着目した。ガンマ－リノレン酸は、このプロスタグランジンンの前駆物質であることから、ガンマ－リノレン酸は、雌のみならず、雄の繁殖能力向上にも寄与するのではないかと想到するに到った。
　そこで、鋭意研究を重ねることで、ガンマ－リノレン酸は、精子活力を向上させ得ることを見いだした。また、このような活性化された精子を用いて雌に種付けを行うことで、雌の受胎率及び分娩回転率が向上することを見いだした。さらに、雄と雌の両方にガンマ－リノレン酸を与えることで、より雌の受胎率及び分娩回転率を向上させ得ることを見いだした。
　すなわち、本発明は、ガンマ－リノレン酸を含有する精子活力向上剤、ガンマ－リノレン酸を含有し、雄に給与することで、該雄の精子を種付けした雌の受胎率を向上させ得る受胎率向上剤、精子活力向上方法、及び受胎率向上方法を提供することを目的とする。 In contrast, the present inventor has focused on the existence of prostaglandin as a therapeutic agent for ED (Erectile Dysfunction). Since gamma-linolenic acid is a precursor of this prostaglandin, it has been thought that gamma-linolenic acid may contribute not only to females but also to male reproductive performance.
Thus, through extensive research, it was found that gamma-linolenic acid can improve sperm vitality. In addition, it has been found that seeding females using such activated sperm improves the conception rate and the labor turnover rate of females. Furthermore, it has been found that by giving gamma-linolenic acid to both males and females, the conception rate and labor turnover rate of females can be improved.
That is, the present invention contains a gamma-linolenic acid-containing sperm vitality-improving agent, gamma-linolenic acid, and a fertilization rate that can improve the conception rate of females seeded with male sperm by feeding them to males. It aims at providing an improving agent, a sperm vitality improvement method, and a conception rate improvement method.
　本発明の精子活力向上剤は、ガンマ－リノレン酸を含有するものとしてある。
　また、本発明の受胎率向上剤は、ガンマ－リノレン酸を含有し、雄に給与することで、該雄の精子を種付けした雌の受胎率を向上させるものとしてある。 The sperm vitality improver of the present invention contains gamma-linolenic acid.
The fertility improving agent of the present invention contains gamma-linolenic acid and is fed to males to improve the fertility of females seeded with male sperm.
　また、本発明の精子活力向上方法は、家畜の雄にガンマ－リノレン酸を給与し、精子活力を向上させる方法としてある。
　また、本発明の受胎率向上方法は、家畜の雄にガンマ－リノレン酸を給与し、該雄の精子を家畜の雌に種付けし、該雌の受胎率を向上させる方法としてある。 The method for improving sperm vitality of the present invention is a method for improving sperm vitality by feeding gamma-linolenic acid to males of domestic animals.
In addition, the method for improving the conception rate of the present invention is a method in which gamma-linolenic acid is fed to a male male and the male sperm is seeded on a female female to improve the conception rate of the female.
　本発明によれば、家畜などの動物の雄の精子活力を向上させることができ、これによって、雌の受胎率や分娩回転率を向上させることが可能となる。 According to the present invention, it is possible to improve the sperm vitality of male animals such as livestock, thereby improving the conception rate and the labor turnover rate of females.
実施例１及び比較例１の実験条件とその結果を示す図である。It is a figure which shows the experimental condition of Example 1 and Comparative Example 1, and its result. 実施例１及び比較例１の実験結果を比較した図である。It is the figure which compared the experimental result of Example 1 and Comparative Example 1. FIG. 実施例２及び比較例２の実験条件とその結果を示す図である。It is a figure which shows the experimental condition of Example 2 and Comparative Example 2, and its result. 実施例３、参考例１及び比較例３の実験条件とその結果を示す図である。It is a figure which shows the experimental conditions of Example 3, Reference Example 1, and Comparative Example 3, and its result.
　以下、本発明の実施形態に係る精子活力向上剤、受胎率向上剤、精子活力向上方法、及び受胎率向上方法について具体的に説明する。 Hereinafter, a sperm vitality improving agent, a conception rate improving agent, a sperm vitality improving method, and a conception rate improving method according to an embodiment of the present invention will be specifically described.
＜精子活力向上剤、精子活力向上方法＞
　本実施形態の精子活力向上剤は、ガンマ－リノレン酸を含有させてなるものである。
　また、本実施形態の精子活力向上方法は、家畜などの動物の雄に、ガンマ－リノレン酸を給与して、その精子活力を向上させるものである。 <Sperm vitality improving agent, sperm vitality improving method>
The sperm vitality improving agent of the present embodiment contains gamma-linolenic acid.
In addition, the sperm vitality improving method of this embodiment is to supply gamma-linolenic acid to male animals such as livestock to improve the sperm vitality.
　「精子活力」は、顕微鏡観察により確認される精子の活動状態を段階評価した狭義の精子活力、精子の生存率、及び生存精子濃度を意味する。
　狭義の精子活力は、以下の実施例では３段階で判定し、「３」が最も活発な前進運動をするもの、「２」が活発な運動をするもの、「１」が微弱な運動をするものとしている。 “Sperm vitality” means the sperm vitality in a narrow sense obtained by grading the sperm activity state confirmed by microscopic observation, the sperm viability, and the viable sperm concentration.
The sperm vitality in the narrow sense is determined in three stages in the following examples, "3" is the most active forward movement, "2" is the active movement, "1" is the weak movement It is supposed to be.
　精子の生存率は、総精子数に対する運動している精子数の割合である。
　一般に優秀な種雄豚を１頭所有していれば、１００頭以上の雌豚を繁殖用に飼育することが可能である。従って、精子活力があり、繁殖能力に優れた雄個体の作出は畜産業の発展に大きく貢献するものである。 Sperm survival is the ratio of the number of sperm moving to the total number of sperm.
Generally, if you own one excellent breeding boar, it is possible to breed more than 100 sows for breeding. Therefore, the production of male individuals having sperm vitality and excellent reproduction ability greatly contributes to the development of the livestock industry.
［ガンマ－リノレン酸］
　ガンマ－リノレン酸（γ－リノレン酸、略称ＧＬＡ）は、炭素数１８のトリ不飽和脂肪酸のひとつである。Ｃ６，Ｃ９，Ｃ１２位にいずれもシス型の二重結合を持つ。オメガ６系脂肪酸（ω６系脂肪酸、１８：３，ｎ－６）に分類される。ガンマ－リノレン酸は、動物の体内でジホモ-γ-リノレン酸（略称ＤＧＬＡ）となり、アラキドン酸（略称ＡＡ）やプロスタグランジン（略称ＰＧ）などの原料となる。 [Gamma-linolenic acid]
Gamma-linolenic acid (γ-linolenic acid, abbreviated as GLA) is one of triunsaturated fatty acids having 18 carbon atoms. All of the C6, C9, and C12 positions have a cis-type double bond. It is classified as an omega-6 fatty acid (ω6 fatty acid, 18: 3, n-6). Gamma-linolenic acid becomes dihomo-γ-linolenic acid (abbreviation DGLA) in the animal body and becomes a raw material for arachidonic acid (abbreviation AA) and prostaglandin (abbreviation PG).
　ガンマ－リノレン酸の供給源は、特に限定されない。例えば、ムコール（Mucor）属、モルティエレラ（Mortierella）属、リゾプス（Rizopus）属等の糸状菌、月見草，ボラージ、黒スグリ、ヘンプ等の植物や、スピルリナ等の藻類、エミュー等の動物などに含まれる油脂から得ることができる。また、ガンマ－リノレン酸は、化学合成によって得ることもでき、市販されているものを使用しても良い。 The source of gamma-linolenic acid is not particularly limited. For example, included in fungi such as Mucor genus, Mortierella genus, and Rizopus genus, plants such as evening primrose, borage, black currant, hemp, algae such as spirulina, and animals such as emu Can be obtained from the oils and fats. In addition, gamma-linolenic acid can be obtained by chemical synthesis, and commercially available products may be used.
　また、ガンマ－リノレン酸は、上記の通り、動物の体内でジホモ-γ-リノレン酸となり、アラキドン酸やプロスタグランジンなどの原料となることから、これらのオメガ６系脂肪酸を、本実施形態においてガンマ－リノレン酸に変えて使用しても、ガンマ－リノレン酸を使用した場合に近い効果が得られるものと推測される。 Further, as described above, gamma-linolenic acid becomes dihomo-γ-linolenic acid in the animal body and becomes a raw material such as arachidonic acid and prostaglandin. Therefore, these omega-6 fatty acids are used in this embodiment. Even if it is used in place of gamma-linolenic acid, it is presumed that an effect close to that obtained when gamma-linolenic acid is used can be obtained.
　さらに、ガンマ－リノレン酸やこれらのオメガ６系脂肪酸の誘導体として、各種アルコール類との反応により得られるエステル、例えばエチルエステル，グリセロールエステル、リン脂質等、あるいは無機，有機の塩基とを、等モル比で作用して得られる塩、例えばナトリウム塩，カリウム塩等を挙げることができる。これらを、本実施形態においてガンマ－リノレン酸に変えて使用しても、ガンマ－リノレン酸を使用した場合に近い効果が得られるものと推測される。 Furthermore, as derivatives of gamma-linolenic acid and these omega-6 fatty acids, equimolar amounts of esters obtained by reaction with various alcohols, such as ethyl esters, glycerol esters, phospholipids, or inorganic or organic bases, are used. Examples thereof include salts obtained by acting in a ratio, such as sodium salt and potassium salt. Even if these are used instead of gamma-linolenic acid in this embodiment, it is presumed that an effect close to that obtained when gamma-linolenic acid is used can be obtained.
　本実施形態の精子活力向上剤による精子活力向上効果は、含有されているガンマ－リノレン酸にもとづき得られるものであるため、当該効果の観点から、精子活力向上剤におけるガンマ－リノレン酸含有割合は高いほど好ましい。
　また、本実施形態の精子活力向上剤において、ガンマ－リノレン酸の他に、各種アミノ酸を含有させてもよい。例えば、アルギニン、リジン、トレオニン、メチオニン、Ｌ－バリン、トリプトファンなどを、ガンマ－リノレン酸と共に含有させることも好ましい。特に、アルギニンは、精液に多く含まれ、精子活力を向上させると言われている。 Since the sperm vitality improving effect by the sperm vitality improving agent of the present embodiment is obtained based on the contained gamma-linolenic acid, from the viewpoint of the effect, the gamma-linolenic acid content ratio in the sperm vitality improving agent is Higher is preferable.
In the sperm vitality improving agent of the present embodiment, various amino acids may be contained in addition to gamma-linolenic acid. For example, arginine, lysine, threonine, methionine, L-valine, tryptophan and the like are preferably contained together with gamma-linolenic acid. In particular, arginine is abundant in semen and is said to improve sperm vitality.
　さらに、本実施形態の精子活力向上剤に、アスタキサンチン、ビタミンＥ、コエンザイムＱ１０、カルニチンを含有させることも好ましい。いずれもエネルギー産生に関わる成分であり、精子活力向上に寄与する可能性があるためである。
　また、本実施形態の精子活力向上剤に、亜鉛やセレンなどのミネラル成分を含有させることも好ましい。 Furthermore, it is also preferable to contain astaxanthin, vitamin E, coenzyme Q10, and carnitine in the sperm vitality improving agent of this embodiment. This is because both are components related to energy production and may contribute to improving sperm vitality.
Moreover, it is also preferable to contain mineral components, such as zinc and selenium, in the sperm vitality improvement agent of this embodiment.
［給与対象動物］
　本実施形態の精子活力向上剤を給与する対象は、家畜などの動物の雄である。「家畜」は、犬、猫、豚、牛、馬、羊、にわとり等の人以外のほ乳類及び鳥類であって、人により飼育されるものとして用いている。また、ヒトに対して投与しても精子活力向上効果があると考えられる。 [Animal subject to salary]
A subject to which the sperm vitality improving agent of the present embodiment is fed is a male animal such as livestock. “Livestock” refers to mammals and birds other than humans, such as dogs, cats, pigs, cows, horses, sheep, and chickens, and is used as those raised by humans. Moreover, even if administered to humans, it is considered that there is an effect of improving sperm vitality.
［給与方法］
　本実施形態の精子活力向上剤を家畜に与える場合、飼料に配合して給与しても、飼料とは別個に与えてもかまわない。また、その他の配合成分と共に給与してもよく、剤型も特に制限されない。ＧＬＡ油を用いる場合、飼料に混ぜたり、ソフトゼラチンカプセルに入れて給与したりすることもできる。 [Salary method]
When the sperm vitality-improving agent of the present embodiment is given to livestock, the sperm vitality-improving agent may be blended with feed and fed separately from the feed. Moreover, it may be fed together with other compounding ingredients, and the dosage form is not particularly limited. When GLA oil is used, it can be mixed with feed or fed in soft gelatin capsules.
［給与期間］
　本実施形態の精子活力向上剤の給与期間は、精子採取の前、最低３日以上とすることが好ましい。これ未満の期間にすると、精子活力が十分に向上しない場合があるためである。 [Salary period]
The feeding period of the sperm vitality improving agent of this embodiment is preferably at least 3 days before sperm collection. This is because if the period is shorter than this, sperm vitality may not be sufficiently improved.
［給与量］
　本実施形態の精子活力向上剤の投与量は、１日当たりのガンマ－リノレン酸の量が、投与対象の家畜の体重の０．０５ｍｇ／ｋｇ～１００ｍｇ／ｋｇとなるようにすることが好ましい。また、１ｍｇ／ｋｇ～１００ｍｇ／ｋｇとすることがより好ましく、１ｍｇ／ｋｇ～５０ｍｇ／ｋｇとすることがさらに好ましく、３ｍｇ／ｋｇ～３０ｍｇ／ｋｇとすることがさらに一層好ましい。例えば、３００ｋｇの種雄豚の場合には、毎朝０．０１５ｇ～３０ｇを好適に投与することができ、０．３ｇ～３０ｇとすることがより好ましく、０．３ｇ～１５ｇとすることがさらに好ましく、０．９ｇ～９ｇとすることがさらに一層好ましい。 [Salary]
The dosage of the sperm vitality improving agent of this embodiment is preferably such that the amount of gamma-linolenic acid per day is 0.05 mg / kg to 100 mg / kg of the body weight of the livestock to be administered. Further, it is more preferably 1 mg / kg to 100 mg / kg, further preferably 1 mg / kg to 50 mg / kg, still more preferably 3 mg / kg to 30 mg / kg. For example, in the case of a 300 kg breed boar, 0.015 g to 30 g can be suitably administered every morning, more preferably 0.3 g to 30 g, still more preferably 0.3 g to 15 g, It is even more preferable to use 0.9 g to 9 g.
　ここで、特許文献１及び３では、雌豚及び雌牛のいずれに対しても、ガンマ－リノレン酸などを含有する飼料を、当該脂肪酸に換算して１日１頭当たり２０ｇ～２００ｇ給与している。
　これに対して、本実施形態の精子活力向上剤の場合、上記の通り、例えば毎朝０．３ｇ～３０ｇ給与することで、十分な効果を得ることができる。 Here, in Patent Documents 1 and 3, a feed containing gamma-linolenic acid or the like is fed to each of sows and cows in an amount of 20 to 200 g per head per day in terms of the fatty acid. .
On the other hand, in the case of the sperm vitality improving agent of the present embodiment, sufficient effects can be obtained by feeding 0.3 g to 30 g, for example, every morning as described above.
＜受胎率向上剤、受胎率向上方法＞
　本実施形態の受胎率向上剤は、ガンマ－リノレン酸を含有し、家畜などの動物の雄に給与することで、該雄の精子を種付けした雌の受胎率を向上させるものである。また、本実施形態の受胎率向上剤として、家畜などの動物の雄と雌の両方に給与して、雄の精子活力を向上させ、かつ、該雄の精子を種付けした雌の受胎率を向上させるものとすることも好ましい。 <Conception rate improver, conception improvement method>
The fertility-enhancing agent of the present embodiment contains gamma-linolenic acid, and is fed to male animals such as livestock to improve the fertility rate of females seeded with male sperm. Moreover, as a fertility improver of this embodiment, it is fed to both males and females of animals such as livestock to improve male sperm vitality and improve the fertility rate of females seeded with the male sperm It is also preferable to make it.
　また、本実施形態の受胎率向上方法は、家畜などの動物の雄にガンマ－リノレン酸を給与し、該雄の精子を雌に種付けし、該雌の受胎率を向上させるものである。また、本実施形態の受胎率向上方法として、家畜などの動物の雄にガンマ－リノレン酸を給与し、雄の精子活力を向上させ、ガンマ－リノレン酸を給与した家畜などの動物の雌に該雄の精子を種付けし、該雌の受胎率を向上させることも好ましい。
　なお、「受胎率」とは、種付けを行った雌の頭数に対する受胎した雌の頭数の割合を意味する。 In addition, the method of improving the fertility rate of the present embodiment is to feed gamma-linolenic acid to a male animal such as a domestic animal, seed the male sperm to a female, and improve the fertility rate of the female. In addition, as a method for improving the fertility rate of this embodiment, gamma-linolenic acid is fed to male animals such as livestock, the sperm vitality of males is improved, and the female females such as domestic animals fed gamma-linolenic acid are treated with gamma-linolenic acid. It is also preferred to seed male sperm to improve the female fertility.
“Conception rate” means the ratio of the number of females with conception to the number of females with seeding.
　本実施形態の受胎率向上剤及び受胎率向上方法において使用するガンマ－リノレン酸は、精子活力向上剤の説明において上述したものと同様のものを用いることができる。また、オメガ６系脂肪酸をガンマ－リノレン酸に変えて使用したり、これらの誘導体をガンマ－リノレン酸に変えて使用してもよく、アルギニンなどのアミノ酸やアスタキサンチン等のその他の配合成分を含有させてもよい。 As the gamma-linolenic acid used in the conception rate improving agent and the conception rate improving method of the present embodiment, the same as those described above in the description of the sperm vitality improving agent can be used. In addition, omega-6 fatty acids may be used in place of gamma-linolenic acid, or these derivatives may be used in place of gamma-linolenic acid, which contains amino acids such as arginine and other compounding ingredients such as astaxanthin. May be.
　また、本実施形態の受胎率向上剤は、家畜などの動物の雄に給与することができる。すなわち、受胎率向上剤を給与されていない雌に対し、受胎率向上剤を給与された雄の精子を用いて種付けを行うことで、雌の受胎率が向上する。これは、受胎率向上剤を給与された雄の精子が活性化されることによるものと考えられる。 Moreover, the fertility improvement agent of this embodiment can be fed to male animals such as livestock. That is, the female fertility rate is improved by seeding a female not fed with a conception rate improving agent using male sperm fed with a fertility rate improving agent. This is thought to be due to the activation of male sperm fed with a fertility enhancer.
　さらに、本実施形態の受胎率向上剤は、家畜などの動物の雄と雌の両方に給与することも好ましい。このようにすれば、ガンマ－リノレン酸を含有する受胎率向上剤により、雌自身の受胎率が向上することに加え、当該受胎率向上剤により雄の精子が活性化されるため、受胎率を格段に向上させることが可能となる。
　なお、本実施形態の受胎率向上剤は、ヒトに対して投与した場合にも効果があるものと考えられる。 Furthermore, it is also preferable to feed the fertility improving agent of this embodiment to both males and females of animals such as livestock. In this way, the fertility improving agent containing gamma-linolenic acid improves the conception rate of the female itself, and the fertility improving agent activates the male sperm. It becomes possible to greatly improve.
In addition, it is thought that the fertility improvement agent of this embodiment is effective also when administered with respect to a human.
　本実施形態の受胎率向上剤の給与方法は、精子活力向上剤の給与方法と同様のものとすることができ、その給与期間も同様のものとすることができる。
　また、雌に対して給与する場合は、種付け前、最低３日以上とすることが好ましい。これ未満の期間にすると、受胎率が十分に向上しない場合があるためである。 The method of feeding the fertility improving agent of this embodiment can be the same as the method of feeding the sperm vitality improving agent, and the feeding period can be the same.
Moreover, when paying | feeding with respect to a female, it is preferable to set it as the minimum 3 days or more before seeding. This is because if the period is shorter than this, the conception rate may not be sufficiently improved.
　さらに、本実施形態の受胎率向上剤の給与量についても、精子活力向上剤と同様に、１日当たりのガンマ－リノレン酸の量が、給与対象の家畜の体重の０．０５ｍｇ／ｋｇ～１００ｍｇ／ｋｇとなるようにすることが好ましい。また、１ｍｇ／ｋｇ～１００ｍｇ／ｋｇとすることがより好ましく、１ｍｇ／ｋｇ～５０ｍｇ／ｋｇとすることがさらに好ましく、３ｍｇ／ｋｇ～３０ｍｇ／ｋｇとすることがさらに一層好ましい。例えば、２００ｋｇの雌豚の場合には、毎朝０．０１ｇ～２０ｇを好適に投与することができ、０．２ｇ～２０ｇとすることがより好ましく、０．２ｇ～１０ｇとすることがさらに好ましく、０．６ｇ～６ｇとすることがさらに一層好ましい。 Further, regarding the amount of the fertility improver of this embodiment, the amount of gamma-linolenic acid per day is 0.05 mg / kg to 100 mg / kg of the weight of the livestock to be fed, as in the case of the sperm vitality improver. It is preferable to make it kg. Further, it is more preferably 1 mg / kg to 100 mg / kg, further preferably 1 mg / kg to 50 mg / kg, still more preferably 3 mg / kg to 30 mg / kg. For example, in the case of a 200 kg sow, 0.01 g to 20 g can be suitably administered every morning, more preferably 0.2 g to 20 g, still more preferably 0.2 g to 10 g, It is even more preferable that the amount be 0.6 g to 6 g.
　「分娩回転率（分娩回転数）」は、次式により算出することができる。不妊の場合は、前回の離乳から発情までの期間が延びるため、回転率が高いほど効率良く分娩できることを意味する。
　分娩回転率＝３６５日÷（妊娠期間１１４日＋泌乳期間（３～４週間）＋離乳から発情期間（４～１０日）の合計（１３９～１５２日）） The “parturition rotation rate (partum rotation number)” can be calculated by the following equation. In the case of infertility, since the period from the previous weaning to estrus is extended, it means that the higher the turnover rate, the better the delivery.
Labor turnover rate = 365 days ÷ (gestation period 114 days + lactation period (3-4 weeks) + weaning to estrus period (4-10 days) (139-152 days))
　以下、本発明の精子活力向上剤、及び精子活力向上方法を用いて、種雄豚に対する精子活力を向上させた実施例、本発明の受胎率向上剤、受胎率向上方法を用いて、雌豚の受胎率と分娩回転率を向上させた実施例に関して説明する。 Hereinafter, using the sperm vitality improving agent of the present invention and the method of improving sperm vitality, examples of improving sperm vitality for breeding boars, the fertility improving agent of the present invention, the method of improving the fertilization rate, An embodiment in which the conception rate and the labor turnover rate are improved will be described.
＜精子活力向上試験＞
（実施例１）
　マルニ印配合飼料マーテル（鹿島飼料株式会社）で飼育されている種雄豚のデュロック種４頭（Ｄ１：体重３００ｋｇ、Ｄ２：体重３２０ｋｇ、Ｄ３：体重２８０ｋｇ、Ｄ４：体重３００ｋｇ）、及び大ヨークシャー種１頭（Ｗ１：体重３００ｋｇ）を対象に、本発明のガンマ－リノレン酸を含有する精子活力向上剤を給与して、精子活力向上試験を行った。 <Sperm vitality improvement test>
Example 1
Four Duloc breeds (D1: body weight 300 kg, D2: body weight 320 kg, D3: body weight 280 kg, D4: body weight 300 kg), and a large Yorkshire species 1 bred in the Marni Mark-containing feed martel (Kashima Feed Co., Ltd.) A sperm vitality improvement test containing gamma-linolenic acid of the present invention was given to the head (W1: body weight 300 kg), and a sperm vitality improvement test was conducted.
　具体的には、それぞれの豚に毎朝１回、ムコール　シルシネロイデス菌体約４０ｇを計量カップで測定し、トップドレスで給与した。ムコール　シルシネロイデス菌体には、ガンマ－リノレン酸含有油脂が含まれ、その含有量は、菌体１ｇ中に、ガンマ－リノレン酸として約７５ｍｇである。したがって、菌体約４０ｇは、ガンマ－リノレン酸の量としては、約３ｇに相当し、各雄豚の体重１ｋｇに対するガンマ－リノレン酸の給与量は、約１０ｍｇとなる。 Specifically, about 40 g of mucor silcineroides cells were measured with a measuring cup once a morning for each pig and fed in a top dress. Mucor silcineroides cells contain gamma-linolenic acid-containing oil and fat, and the content thereof is about 75 mg as gamma-linolenic acid in 1 g of cells. Therefore, about 40 g of cells correspond to about 3 g of gamma-linolenic acid, and the amount of gamma-linolenic acid fed to 1 kg of body weight of each boar is about 10 mg.
　給与期間は、採取するサンプル数によって異なり、その数に応じて長くなる。例えば、デユロックＤ１では、６回の採取（サンプル数６）を行っており、最初の採取は、給与開始後５日目、２回目が１９日目、３回目が３３日目である。最後の採取は、給与開始後７５日目であり、合計７５日間給与した。
　また、給与開始後５日目から約２週間に１回の割合で、各豚を擬牝台に乗せ、精液を採取した。採取後、精液検査を実施した。精液検査は、顕微鏡的検査により行った。以上の結果を図１に示す。 The salary period varies depending on the number of samples to be collected, and becomes longer depending on the number. For example, at Deyulock D1, six times of sampling (6 samples) are performed, and the first time of sampling is the fifth day after the start of salary, the second time is the 19th day, and the third time is the 33rd day. The last collection was 75 days after the start of salary, and the salary was paid for a total of 75 days.
In addition, from the fifth day after the start of feeding, each pig was placed on a pseudo-female about once every two weeks and semen was collected. After collection, a semen test was performed. Semen examination was performed by microscopic examination. The above results are shown in FIG.
　なお、同図において、サンプル数は、精液を採取した容器数を示しており、採取回数に対応する。また、採取した精液を１ボトル当たりに約５５億個の精子が含まれるように保存液で希釈し、精液ボトルを作成した。 In the figure, the number of samples indicates the number of containers from which semen was collected, and corresponds to the number of collections. Further, the collected semen was diluted with a storage solution so that about 5.5 billion spermatozoa were contained per bottle, and a semen bottle was prepared.
　平均精子活力は、精子の活動状態を段階評価したものの平均値であり、最も活発な前進運動をおこなうものを「３」、活発な運動をするものを「２」、微弱な運動をするものを「１」として算出している。
　平均生存率は、各サンプルにおける運動している精子の割合の平均値である。生存率は、血球計算盤を用いて、運動している精子数を測定し、総精子数に対する割合を算出して得た。
　平均生存精子濃度は、採取精液１ｍｌあたりの平均生存精子数をいう。 The average sperm vitality is the average value of the graded sperm activity, “3” for the most active forward movement, “2” for the active movement, and weak movement. It is calculated as “1”.
Average survival is the average percentage of moving sperm in each sample. The survival rate was obtained by measuring the number of moving spermatozoa using a hemocytometer and calculating the ratio to the total sperm count.
The average viable sperm concentration refers to the average viable sperm number per 1 ml of collected semen.
（比較例１）
　実施例１と同じ雄豚について、実施例１を行う前に、ガンマ－リノレン酸を含有する精子活力向上剤を給与する点を除いて同様の条件で飼育し、精液検査を実施して、その精子活力を確認した。その結果を図１に示す。 (Comparative Example 1)
The same boar as in Example 1 was raised under the same conditions except that a sperm vitality improver containing gamma-linolenic acid was fed before performing Example 1, and a semen test was conducted. Sperm vitality was confirmed. The result is shown in FIG.
　図２は、実施例１及び比較例１における同一の雄豚毎に、平均精子活力、平均生存率、平均生存精子濃度、及び濃度増加率を示したものである。
　同図に示されるように、デュロック１，２については、比較例１－１，１－２の平均精子活力がいずれも２．８であり、比較的高い数値を示していたが、実施例１－１，１－２では、いずれも３．０となり、精子活力が一層向上している。また、デュロック３，４及び大ヨークシャーについては、比較例１－３，１－４，１－５の平均精子活力がいずれも２．３であり、比較的低い数値を示していたが、実施例１－４では２．８に、実施例１－３，１－５では３．０になり、いずれも精子活力が大きく向上している。 FIG. 2 shows the average sperm vitality, average survival rate, average survival sperm concentration, and concentration increase rate for each identical boar in Example 1 and Comparative Example 1.
As shown in the figure, for Duroc 1 and 2, the average sperm vitality of Comparative Examples 1-1 and 1-2 was 2.8, showing relatively high values. In both cases of -1 and 1-2, the sperm vitality is further improved to 3.0. Further, for Duroc 3 and 4 and Large Yorkshire, the average sperm vitality of Comparative Examples 1-3, 1-4, and 1-5 were both 2.3, indicating relatively low values. 1-4 was 2.8, and Examples 1-3 and 1-5 were 3.0, which significantly improved sperm vitality.
　また、平均生存率についても、個体毎の実施例及び比較例を比較すると、いずれも３～６％の増加を示している。
　さらに、平均生存精子濃度については、個体間で大きなばらつきがあるものの、実施例の結果は、いずれも比較例に対して、十％程度から数十％程度増加している。特に、デュロック１，４と大ヨークシャーでは、平均生存精子濃度が著しく増加している。 Further, the average survival rate also shows an increase of 3 to 6% when comparing the examples and comparative examples for each individual.
Furthermore, although the average viable sperm concentration varies greatly among individuals, the results of the examples are all increased by about 10% to several tens of% with respect to the comparative examples. In particular, the average viable sperm concentration is significantly increased in Duroc 1 and 4 and Large Yorkshire.
＜受胎率向上試験＞
１．雄豚へのＧＬＡ給与効果の確認試験
（実施例２）
　マルニ印配合飼料マーテルで飼育されている種雄豚のデュロック種Ｄ５（体重３００ｋｇ）に対し、毎朝１回、ムコール　シルシネロイデス菌体約４０ｇを計量カップで測定し、トップドレスで給与した。給与量は、実施例１と同様に、ガンマ－リノレン酸の量として約３ｇであり、このデュロックＤ５の体重１ｋｇあたりのガンマ－リノレン酸の給与量は、約１０ｍｇであった。
　給与開始後７日目に、この豚を擬牝台に乗せ、精液を採取した。採取後、精液検査を実施した。精液検査は、顕微鏡的検査により行った。その結果、精子活力は３、生存率は８５％であった。 <Conception rate improvement test>
1. Confirmation test of GLA feeding effect on boar (Example 2)
About 40 g of mucor silcineroides cells were measured with a measuring cup once a morning for Duroc D5 (body weight 300 kg) of a breeding boar bred on Marni-mark mixed feed Martell and fed in a top dress. As in Example 1, the amount of gamma-linolenic acid was about 3 g as in Example 1, and the amount of gamma-linolenic acid per kg body weight of Duroc D5 was about 10 mg.
Seven days after the start of salary, the pig was placed on a pseudo-female and semen was collected. After collection, a semen test was performed. Semen examination was performed by microscopic examination. As a result, the sperm vitality was 3, and the survival rate was 85%.
　次に、この精液を使用し、大ヨークシャー（父系）－ランドレース（母系）種の雌豚２０頭に対して種付けを行った。これらの雌豚は、ガンマ－リノレン酸を給与することなく、マルニ印配合飼料マーテルにより飼育した。
　種付けは、発情鑑定を行うために雄豚による本交配を行い、翌日午前と午後の２回、人口受精を行った。人口受精は、デュロックＤ５から採取して得られた精液ボトルを使用し、精液ボトルからカテーテルを用いて実施した。精液ボトルは、１ボトル当たりに約５５億個の精子が含まれるように保存液で希釈して作成し、１回の人口受精で１ボトルを使用した。
　その結果、図３に示すように、受胎頭数は２０頭、受胎率は１００％であった。 Next, using this semen, seeding was carried out on 20 sows of large Yorkshire (paternal) -Landrace (maternal) breed. These sows were bred with Marni-marked feed martels without feeding gamma-linolenic acid.
For seeding, this mating was carried out with boars for estrus assessment, and population fertilization was conducted twice in the morning and afternoon the next day. Population fertilization was performed using a semen bottle obtained from Duroc D5 and using a catheter from the semen bottle. Semen bottles were prepared by diluting with a preservative solution so that approximately 5.5 billion spermatozoon were contained per bottle, and one bottle was used for one-time fertilization.
As a result, as shown in FIG. 3, the number of conception heads was 20, and the conception rate was 100%.
（比較例２）
　実施例２と同じ雄豚のデュロックＤ５を、実施例２を行う前に、ガンマ－リノレン酸の給与を行うことなく、その他の点は実施例２と同様の条件でマルニ印配合飼料マーテルにより飼育し、精液検査を実施して、その精子活力を確認した。その結果、精子活力は２、生存率は８０％であった。
　この精液を使用して、実施例２と同様に、大ヨークシャー（父系）－ランドレース（母系）種の雌豚２０頭に対して種付けを行った。これらの雌豚は、ガンマ－リノレン酸を含有していないマルニ印配合飼料マーテルを用いて、実施例２と同様に飼育されたものである。
　その結果、図３に示すように、受胎頭数は１８頭、受胎率は９０％であった。 (Comparative Example 2)
The same boar Duroc D5 as in Example 2 was raised on the Marni-marked mixed feed martel under the same conditions as in Example 2 except that gamma-linolenic acid was not fed before Example 2. Then, a semen test was performed to confirm the sperm vitality. As a result, the sperm vitality was 2, and the survival rate was 80%.
Using this semen, as in Example 2, 20 large sows of the large Yorkshire (paternal) -Landlace (maternal) breed were seeded. These sows were bred in the same manner as in Example 2 using marni-marked mixed feed martel that does not contain gamma-linolenic acid.
As a result, as shown in FIG. 3, the number of conception heads was 18, and the conception rate was 90%.
２．雄豚及び雌豚へのＧＬＡ給与効果の確認試験
（実施例３）
　マルニ印配合飼料マーテルで飼育されている体重１８０～２００ｋｇの雌豚の体重のランドレース－大ヨークシャー種１００頭、及び大ヨークシャー－ランドレース種１０３頭を対象に、実施例１で採取した精液を使用して、種付けを行った。
　これらの雌豚には、上記飼料にガンマ－リノレン酸を含有させて給与した。具体的には、飼料の給与量は、分娩前７日間は４ｋｇ／日であり、各雌豚の飼料に０．２ｗｔ％となるように、実施例１と同じムコール　シルシネロイデス菌体を配合した。したがって、同菌体の配合量は８ｇであり、菌体１ｇ中にガンマ－リノレン酸は約７５ｍｇ含まれるため、菌体８ｇはガンマ－リノレン酸の量としては約６００ｍｇに相当し、各雌豚の体重１ｋｇに対するガンマ－リノレン酸の給与量は、約３ｍｇである。なお、分娩から離乳後７日までの飼料の給与量は６ｋｇ／日であり、各雌豚の飼料に０．０８ｗｔ％となるようにムコール　シルシネロイデス菌体を配合した。 2. Confirmation test of GLA feeding effect on boar and sow (Example 3)
The semen collected in Example 1 was used for land races of 100-200 kg sows with a weight of 180-200 kg, which were bred in the Marni Mark mixed feed Martell-100 large Yorkshire species and 103 large Yorkshire-Landlace species. Used for seeding.
These sows were fed with gamma-linolenic acid in the feed. Specifically, the feed amount of feed was 4 kg / day for 7 days before parturition, and the same mucor silcineroides cells as in Example 1 were blended in the feed of each sow to 0.2 wt%. Therefore, the amount of the microbial cells is 8 g, and about 75 mg of gamma-linolenic acid is contained in 1 g of the microbial cells. Therefore, 8 g of microbial cells corresponds to about 600 mg as the amount of gamma-linolenic acid. The amount of gamma-linolenic acid fed per kg body weight is about 3 mg. In addition, the feed amount of feed from parturition to 7 days after weaning was 6 kg / day, and Mucor silcineroides cells were blended in the feed of each sow to 0.08 wt%.
　また、種付けは、実施例２と同様に、本交配と人口受精により行った。人口受精には、実施例１のデュロック種（Ｄ１，Ｄ２，Ｄ３，Ｄ４）の雄豚から採取して得られた精液ボトルを使用した。
　その結果、図４に示すように、ランドレース－大ヨークシャー種１００頭中、９６頭が受胎し、大ヨークシャー－ランドレース種１０３頭中、１００頭が受胎し、合計受胎頭数は１９６頭、受胎率は９７％であった。試験豚の中には不妊等の個体固有の欠点を含む雌豚も存在するため、９７％は最高レベルの受胎率と考えられる。また、実施例３における平均分娩回転率は、２．４６であった。 Moreover, seeding was performed by this crossing and artificial fertilization as in Example 2. For artificial fertilization, semen bottles obtained from the Duroc breed (D1, D2, D3, D4) boar of Example 1 were used.
As a result, as shown in FIG. 4, 96 out of 100 Landrace-Large Yorkshire species were conceived, 100 out of 103 large Yorkshire-Landrace species were conceived, and the total number of conceived heads was 196. The rate was 97%. 97% is considered to be the highest conception rate because there are some sows that contain individual defects such as infertility among the test pigs. The average labor turnover rate in Example 3 was 2.46.
３．雌豚へのＧＬＡ給与効果の確認試験
（参考例１）
　マルニ印配合飼料マーテルで飼育されている体重１８０～２００ｋｇの雌豚の体重のランドレース－大ヨークシャー種４４頭、及び大ヨークシャー－ランドレース種４０頭を対象に、比較例１で採取された、ＧＬＡによる活性化が行われていない精液を使用して、種付けを行った。
　各雌豚の飼料には、実施例３と同様に、ガンマ－リノレン酸を含有させて、分娩前７日間は毎日４ｋｇ給与した。各雌豚の体重１ｋｇに対するガンマ－リノレン酸の給与量は、約３ｍｇである。なお、分娩から離乳後７日までの飼料の給与量は６ｋｇ／日であり、各雌豚の飼料に０．０８ｗｔ％となるようにムコール　シルシネロイデス菌体を配合した。 3. Confirmation test of GLA feeding effect on sows (Reference Example 1)
Samples were collected in Comparative Example 1 for Land Race-44 large Yorkshire breeds and 40 Large Yorkshire-Land Race breeds weighing 180-200 kg sows bred in Marni-mark mixed feed Martell. Seeding was performed using semen that had not been activated by GLA.
As in Example 3, each sow feed contained gamma-linolenic acid and was fed 4 kg daily for 7 days before parturition. The amount of gamma-linolenic acid fed to 1 kg body weight of each sow is about 3 mg. In addition, the feed amount of feed from parturition to 7 days after weaning was 6 kg / day, and Mucor silcineroides cells were blended in the feed of each sow so as to be 0.08 wt%.
　また、種付けは、実施例３と同様に、本交配と人口受精により行った。人口受精には、比較例１のデュロック種（Ｄ１）の雄豚から採取して得られた精液ボトルを使用した。
　その結果、図４に示すように、ランドレース－大ヨークシャー種４４頭中、４０頭が受胎し、大ヨークシャー－ランドレース種４０頭中、３８頭が受胎し、合計受胎頭数は７８頭、受胎率は９３％であった。また、参考例１における平均分娩回転率は、２．３６であった。 In addition, seeding was performed by this crossing and artificial fertilization as in Example 3. For artificial fertilization, a semen bottle obtained from the Duroc (D1) boar of Comparative Example 1 was used.
As a result, as shown in FIG. 4, 40 out of 44 Landrace-Largeshire species were conceived, 38 out of 40 large Yorkshire-Landrace species were conceived, and the total number of conceived heads was 78. The rate was 93%. Moreover, the average labor turnover rate in Reference Example 1 was 2.36.
（比較例３）
　マルニ印配合飼料マーテルで飼育されている体重１８０～２００ｋｇの雌豚のランドレース（父系）－大ヨークシャー（母系）種１７０頭、及び大ヨークシャー（父系）－ランドレース（母系）種１７０頭を対象に、比較例１で採取された、ＧＬＡによる活性化が行われていない精液を使用して、種付けを行った。
　各雌豚の飼料には、ガンマ－リノレン酸を含有させなかった。分娩前７日間の飼料の給与量は４ｋｇ／日であり、分娩から離乳後７日までの飼料の給与量は６ｋｇ／日であった。 (Comparative Example 3)
180-200kg sow land races (paternal) – 170 large Yorkshire (maternal) breeds, and 170 large Yorkshire (paternal) – land race (maternal) breeds raised on Martelli Marked feed Martell In addition, seeding was performed using the semen collected in Comparative Example 1 and not activated by GLA.
Each sow diet contained no gamma-linolenic acid. The amount of feed fed for 7 days before parturition was 4 kg / day, and the amount of feed fed from parturition to 7 days after weaning was 6 kg / day.
　種付けは、参考例１と同様に、本交配と人口受精により行った。人口受精には、比較例１のデュロック種（Ｄ１、Ｄ２、Ｄ３、Ｄ４）の雄豚から採取して得られた精液ボトルを使用した。
　その結果、図４に示すように、ランドレース－大ヨークシャー種１７０頭中、１４０頭が受胎し、大ヨークシャー－ランドレース種１７０頭中、１４９頭が受胎し、合計受胎頭数は２８９頭、受胎率は８５％であった。また、比較例３における平均分娩回転率は、２．１５であった。 As in Reference Example 1, seeding was performed by this crossing and artificial fertilization. For artificial fertilization, semen bottles obtained from Dullock (D1, D2, D3, D4) boars of Comparative Example 1 were used.
As a result, as shown in FIG. 4, 140 out of 170 landrace-large Yorkshire species were conceived, 149 out of 170 large yorkshire-landrace species were conceived, and the total number of conceived heads was 289. The rate was 85%. The average labor turnover rate in Comparative Example 3 was 2.15.
　図３の比較例２に示されるように、ガンマ－リノレン酸を給与していない雌豚に対して、ガンマ－リノレン酸を給与していない雄豚の精子を用いて種付けした場合、その受胎率は９０％であった。
　これに対して、実施例２に示されるように、ガンマ－リノレン酸を給与していない雌豚に対して、ガンマ－リノレン酸を給与した雄豚の精子を用いて種付けした場合、その受胎率は１００％となり、１０％向上している。 As shown in Comparative Example 2 in FIG. 3, when a sow that did not receive gamma-linolenic acid was seeded with sperm from a boar that did not receive gamma-linolenic acid, the conception rate Was 90%.
In contrast, as shown in Example 2, when a sow that was not fed gamma-linolenic acid was seeded with sperm from a boar that was fed gamma-linolenic acid, the conception rate was high. Is 100%, an improvement of 10%.
　また、図４の比較例３に示されるように、ガンマ－リノレン酸を給与していない雌豚に対して、ガンマ－リノレン酸を給与していない雄豚の精子を用いて種付けした場合、その受胎率は、８５％であった。
　これに対して、参考例１に示されるように、ガンマ－リノレン酸を給与した雌豚に対して、ガンマ－リノレン酸を給与していない雄豚の精子を用いて種付けした場合、その受胎率は９３％となり、比較例３の結果に比べ８％向上している。
　さらに、実施例３に示されるように、ガンマ－リノレン酸を給与した雌豚に対して、ガンマ－リノレン酸を給与した雄豚の精子を用いて種付けした場合、その受胎率は９７％となり、比較例３の結果に比べ１２％向上している。 In addition, as shown in Comparative Example 3 of FIG. 4, when sows that did not receive gamma-linolenic acid were seeded with sperm from a male pig that did not receive gamma-linolenic acid, The conception rate was 85%.
In contrast, as shown in Reference Example 1, when a sow fed with gamma-linolenic acid was seeded with sperm from a boar not fed gamma-linolenic acid, the conception rate Is 93%, which is 8% higher than the result of Comparative Example 3.
Furthermore, as shown in Example 3, when the sows fed gamma-linolenic acid were seeded with sperm from boars fed gamma-linolenic acid, the conception rate was 97%, Compared to the result of Comparative Example 3, the improvement is 12%.
　また、図４に示されるように、平均分娩回転率は、比較例３が２．１５、参考例１が２．３６、実施例３が２．４６となっている。 Further, as shown in FIG. 4, the average labor turnover rate is 2.15 in Comparative Example 3, 2.36 in Reference Example 1, and 2.46 in Example 3.
　これらの受胎率及び分娩回転率にもとづいて、１０００頭の母豚を飼育する農場における１年後の計算上の頭数を以下に示す。以下の計算では、母豚１頭あたりの子豚の出荷頭数を１０頭とする。
　比較例３の１年後の出荷頭数＝１０００×１０×０．８５×２．１５＝１８，２７５頭
　参考例１の１年後の出荷頭数＝１０００×１０×０．９３×２．３６＝２１，９４８頭
　実施例３の１年後の出荷頭数＝１０００×１０×０．９７×２．４６＝２３，８６２頭 Based on these conception rates and labor turnover rates, the calculated number of heads after one year on a farm that bred 1000 mother pigs is shown below. In the following calculation, the number of piglets shipped per mother pig is assumed to be 10.
Number of shipments after 1 year of Comparative Example 3 = 1000 × 10 × 0.85 × 2.15 = 18,275 Number of shipments after 1 year of Reference Example = 1000 × 10 × 0.93 × 2.36 = 21,948 heads Number of heads shipped in one year after Example 3 = 1000 × 10 × 0.97 × 2.46 = 23,862 heads
　よって、参考例１の受胎率及び分娩回転率によれば、１年後の頭数は、比較例３の場合よりも、３，６７３頭増加することがわかる。また、実施例３の受胎率及び分娩回転率によれば、１年後の頭数は、参考例１の場合よりも、１，９１４頭増加し、比較例３の場合よりも、５，５８７頭増加することがわかる。 Therefore, according to the conception rate and the labor turnover rate in Reference Example 1, it can be seen that the number of heads after one year is increased by 3,673 compared to the case of Comparative Example 3. Moreover, according to the conception rate and the labor turnover rate of Example 3, the number of heads after one year increased by 1,914 heads compared to the case of Reference Example 1, and 5,587 heads compared with the case of Comparative Example 3. It can be seen that it increases.
　また、受胎率及び分娩回転率を向上させることで、受精が失敗することにより生じる、次回の発情まで母豚に必要な飼料給与を低減させることが可能となる。
　さらに、精子活力、及び精子生存濃度が向上することにより、一頭の種雄豚から活力のある精子がより多く得られるとともに、人口受精用の精子の本数がより多く得られ、種雄の飼育頭数を減らすことも可能となり、飼育コストを低減させることが可能となる。 Moreover, by improving the conception rate and the labor turnover rate, it becomes possible to reduce the feed supply necessary for the mother pig until the next estrus, which occurs when fertilization fails.
Furthermore, by improving the sperm vitality and the sperm survival concentration, more vigorous sperm can be obtained from one breeding pig, more sperm for artificial fertilization can be obtained, and the number of breeding breeds can be reduced. This also makes it possible to reduce breeding costs.
　本発明は、以上の実施形態や実施例に限定されるものではなく、本発明の範囲内において、種々の変更実施が可能であることは言うまでもない。
　例えば、上記の実施例では、豚を使用しているが、牛や馬、鳥などの家畜でも同様に適用することが可能である。また、ヒトに対する効果も期待することができる。 The present invention is not limited to the above-described embodiments and examples, and it goes without saying that various modifications can be made within the scope of the present invention.
For example, in the above embodiment, pigs are used, but the same can be applied to livestock such as cows, horses, and birds. In addition, an effect on humans can be expected.
　本発明は、家畜などの動物を効率的に繁殖するために、好適に利用することが可能である。 The present invention can be suitably used for efficiently breeding animals such as livestock.

Claims (6)

  1. 　ガンマ－リノレン酸を含有することを特徴とする精子活力向上剤。 Sperm vitality improver characterized by containing gamma-linolenic acid.
  2. 　ガンマ－リノレン酸を含有し、雄に給与することで、該雄の精子を種付けした雌の受胎率を向上させることを特徴とする受胎率向上剤。 A fertility improving agent characterized by containing gamma-linolenic acid and improving the conception rate of females seeded with male sperm by feeding to males.
  3. 　雄と雌の両方に給与して、雄の精子活力を向上させ、かつ、該雄の精子を種付けした雌の受胎率を向上させる請求項２記載の受胎率向上剤。 3. A fertility enhancer according to claim 2, which is fed to both males and females to improve male sperm vitality and to improve the fertility rate of females seeded with male sperm.
  4. 　家畜の雄にガンマ－リノレン酸を給与し、精子活力を向上させることを特徴とする精子活力向上方法。 A method for improving sperm vitality, characterized in that gamma-linolenic acid is fed to livestock males to improve sperm vitality.
  5. 　家畜の雄にガンマ－リノレン酸を給与し、該雄の精子を家畜の雌に種付けし、該雌の受胎率を向上させることを特徴とする受胎率向上方法。 A method for improving the conception rate, characterized in that gamma-linolenic acid is fed to a domestic male, the male sperm is seeded on a domestic female, and the conception rate of the female is improved.
  6. 　家畜の雄にガンマ－リノレン酸を給与し、雄の精子活力を向上させ、ガンマ－リノレン酸を給与した家畜の雌に該雄の精子を種付けし、該雌の受胎率を向上させることを特徴とする受胎率向上方法。 Featuring gamma-linolenic acid to males in domestic animals to improve male sperm vitality, and seeding male sperm into females fed gamma-linolenic acid to improve the conception rate of females A method for improving the conception rate.
PCT/JP2010/006209 2009-10-20 2010-10-20 Agent for improving sperm motility, agent for improving fertility, method for improving sperm motility and method for improving fertility WO2011048806A1 (en)

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CN103431248A (en) * 2013-09-11 2013-12-11 兴安县嘉鑫养殖农民专业合作社 Chinese herbal medicine feed additive capable of improving quality of gander semen
WO2018132838A3 (en) * 2017-01-15 2019-02-28 Arex Life Sciences Methods for improving fertility in artificial insemination
CN113975301A (en) * 2021-11-24 2022-01-28 暨南大学 Probiotic preparation for improving sperm motility and application thereof

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WO2001097802A1 (en) * 2000-06-17 2001-12-27 Jsr Clover Ltd Supplement to enhance fertility

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Publication number Priority date Publication date Assignee Title
CN103431248A (en) * 2013-09-11 2013-12-11 兴安县嘉鑫养殖农民专业合作社 Chinese herbal medicine feed additive capable of improving quality of gander semen
WO2018132838A3 (en) * 2017-01-15 2019-02-28 Arex Life Sciences Methods for improving fertility in artificial insemination
CN113975301A (en) * 2021-11-24 2022-01-28 暨南大学 Probiotic preparation for improving sperm motility and application thereof

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