WO2011042253A1 - Dispositif de biodétection équipé d'un système de test automatique - Google Patents

Dispositif de biodétection équipé d'un système de test automatique Download PDF

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Publication number
WO2011042253A1
WO2011042253A1 PCT/EP2010/062480 EP2010062480W WO2011042253A1 WO 2011042253 A1 WO2011042253 A1 WO 2011042253A1 EP 2010062480 W EP2010062480 W EP 2010062480W WO 2011042253 A1 WO2011042253 A1 WO 2011042253A1
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WIPO (PCT)
Prior art keywords
detection
test
measuring
biosensor
pressure
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PCT/EP2010/062480
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German (de)
English (en)
Inventor
Andreas Helwig
Christoph Heller
Ulrich Reidt
Alois Friedberger
Thorsten Otto
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Eads Deutschland Gmbh
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Application filed by Eads Deutschland Gmbh filed Critical Eads Deutschland Gmbh
Publication of WO2011042253A1 publication Critical patent/WO2011042253A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00594—Quality control, including calibration or testing of components of the analyser
    • G01N35/00712—Automatic status testing, e.g. at start-up or periodic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00594—Quality control, including calibration or testing of components of the analyser
    • G01N35/00613—Quality control
    • G01N35/00623—Quality control of instruments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00425—Heating or cooling means associated with pipettes or the like, e.g. for supplying sample/reagent at given temperature
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Definitions

  • the invention relates to a biosensor device for detecting biological particles, which has a fluidic device for conducting fluids and has a detection device for detecting the biological particles.
  • the fluidic device has a plurality of fluidic elements and the detection device has a plurality of detector elements.
  • Such a detection method is described for example in DE 10 2007 021 387 A1.
  • the biological particles are filtered out by means of a filter from the sample to be examined.
  • Fabrics and chemicals needed for different detection steps, such as marking steps to label the particles are directed via a fluidic device to and from the filter.
  • the radiation of the probes marking the particles is detected, with which the particles can be detected.
  • the detection method should be performed automatically.
  • the individual components of the biosensor device are functional,
  • the object of the invention is therefore to provide a biosensor device for the detection of biological particles, with which an automatic detection can be performed reliably.
  • the functional test can also be carried out automatically.
  • a biosensor device especially when it is used for the investigation of drinking water, a functional monitoring is desirable. Furthermore, the drinking water should not be contaminated in case of malfunction. This is achieved in a preferred embodiment of the invention.
  • the biosensor device preferably has a fluidic device, which conducts fluids in the biosensor device and transports them to the intended locations.
  • a detection device in which the biological particles are detected.
  • the fluidic device has at least one fluidic element, preferably a plurality of fluidic elements, and the detection device has at least one detector element, preferably a plurality of detector elements.
  • a self-test device is provided which automatically tests these components.
  • the self-test device preferably uses the components already present in the biosensor device in order to monitor the state of the components as well as the overall system by means of specific sequence programs. Thus, a system monitoring is possible, with no significant expansion of the system is required by additional monitoring devices.
  • the self-test device has at least one test device which is designed for testing the fluidic elements and / or the detector elements. Further preferably, an evaluation device is provided in the self-test device, which receives test signals from the test device and evaluate them, d. H. can judge.
  • USB universal serial bus
  • the USB components are connected via USB connections to the evaluation device.
  • the evaluation device can receive and further process test signals or general signals of the test device and / or the fluidic elements and / or the detector elements.
  • the evaluation device is formed by software which is to be loaded or loaded in a computer unit.
  • the software can then automatically perform the evaluation of the received test signals from the USB devices by means of the evaluation.
  • the self-test device is started by an initialization step of the software. Since the biosensor device is preferably designed for recording samples on its own, it is advantageous if the self-test device is initialized prior to each of such sample pick-ups by starting the software of the computer unit. This means, e.g. Before the biosensor device picks up a sample and starts a measurement, the entire system is tested via the software initialization step. During the initialization step, the USB components can be tested for their functionality and confirm their operational readiness. In addition to using USB ports, however, other interfaces may be used.
  • the self-test device is designed to be able to determine a malfunction of at least one fluidic element and / or at least one detector element. If the self-test device has detected a malfunction in one of the components of the biosensor device, it is advantageously designed to switch off the biosensor device. Thus, further damage or disturbance of the individual components can be avoided and the contamination of the sample source can be prevented.
  • the probes for labeling the biological particles may be present, which attach to the biological particles when the detection fluid is passed through the filter on which the biological particles are immobilized.
  • Cleaning fluids are designed to clean the filter after detection by removing the biological particles that have been immobilized there from the pores.
  • a selection valve is advantageously an eight-way valve with which at least eight different detection and / or cleaning fluids can be selected. Such a selection valve results in a large margin of the available fluids.
  • the fluids, d. H. the detection and / or cleaning fluids transported via a fluidic element in the fluidic device is preferably a pump which pumps the detection and / or cleaning fluids.
  • the pump is a precision micropump.
  • a precision micropump is in particular a pump of reduced size, which is used for microsystems, but has similar flow rates as the classical pumps.
  • a micro-pump can be easily integrated in the reduced space available. Since only very small volumes are pumped, it is advantageous if the micropump is a precision micropump to accurately pump very small volumes.
  • the selection valve which is preferably a multi-way valve, in particular an eight-way valve, connects the pump with the detection and / or cleaning fluids.
  • the pump can precisely pump the detection and / or cleaning fluid to the filter that is needed, be it for detection or for cleaning.
  • a fluid identification device is provided as a test device in the self-test device or as one of several test devices. see. With this fluid identification device, it is possible to detect which detection and / or cleaning fluid is involved.
  • the fluid identification device advantageously has, for example, at least one power measuring device which can measure the power of the pump. If the fluid identification device further advantageously has a rotational speed measuring device of the pump motor axis, and is designed such that it has a rotational speed measurement device.
  • / Power consumption characteristic can create and evaluate, it can calculate from this characteristic, which viscosity has the fluid in question. Thereby it can be identified which fluid is being pumped by the detection and / or cleaning fluids just from the pump.
  • the selection valve d. H. e.g. the multi-way valve such. the eight-way valve
  • the different process fluids with the pump For example, depending on the step being performed in the biosensor device, a particular detection and / or cleaning fluid is introduced into the biosensor device. This may be, for example, a buffer solution or a cleaning solution or the like.
  • the multiway valve e.g. If the eight-way valve is a USB device, it will cause a software failure if it is not operational or is not powered.
  • the selection valve should successfully sequentially connect to all (e.g., eight) input channels.
  • the selector valve may lose the information as to which channel it is currently dialing; H. the channel number does not correspond to the actual selected channel. Therefore, it is advantageous if it can be determined which fluid has just been selected by the selection valve.
  • the different process fluids have different physical and chemical properties. Therefore, it is possible to select the actually selected channel via, for example, the viscosity of the process fluid determine. If the viscosity of two or more channels is compared, the correct information of the selected channel can be identified. As described above, the viscosity of a fluid can be calculated from the rotational speed of the pump and its power consumption. The delivery of liquids with higher viscosities requires a higher power consumption at the same rotational speeds. For this viscosity test, the pump already provided in the biosensor device can be used. Additional sensors are not needed for this.
  • the conductivity which is an easily measured quantity, can be used for identification.
  • the fluid identification device alternatively or additionally on a conductivity measuring device. Therefore, a conductivity test can be used to identify the selected valve channel, if additionally or alternatively, a conductivity measuring device is provided.
  • the fluid identification device alternatively or additionally has a pH value measuring device for measuring the pH.
  • the pH values of the process fluids differ and the fact that a pH value measuring device is provided allows the selected valve channel to be determined. For this purpose, only the pH of the detection and / or cleaning fluid must be measured.
  • an optical detection unit is alternatively or additionally provided in the fluid identification device.
  • the optical density of the detection and / or cleaning fluid can be measured. Since the different process fluids have different optical densities due to their chemical composition, an optical detection unit can measure the optical density and thus identify the correct selected channel.
  • at least one vessel is provided as the fluidic element or as one of several fluidic elements. In this vessel or in these vessels, the detection and / or cleaning fluids can be stored so that they are always available for the Detetkions processes and / or for the cleaning process.
  • the fluidic identification device preferably has at least one pressure and / or level measuring device, the pressure and / or level in the vessel can be measured. With such a structure, it is possible to determine the selected channel, since the pressure and / or level measuring device indicates a change in pressure and / or level when the selection valve selects the vessel with the pressure and / or level gauge and so fluid is taken from it.
  • the fluid identification device is designed such that it stores the test signal of the pressure and / or level measuring device. This can be determined from the vessels even at several sampling steps, where just a fluid is removed. It is always selected exactly the vessel in which the pressure and / or level changes. In order to detect a change, the previous state must be known, which is possible via a storage of the previous test signal.
  • a pump function monitoring device is provided as a test device or as one of several test devices.
  • the precision micropump is responsible for transporting the process fluids through the biosensor device. It is preferably a USB device and then causes a software error if its control can not be initialized and after a certain time issues a signal if the controller does not recognize the pump. Possible errors of the precision micropump are a broken connection or a jammed rotor or other pump actuator.
  • the pump function monitoring device has the flow measuring device or at least one of a plurality of flow measuring devices. If the pump function monitoring device further preferably has the power measuring device, it is possible to detect and evaluate a power consumption / flow rate characteristic.
  • the precision micropump carries its own self-test procedure in its functionality.
  • the pump function monitoring device has a rotational speed measuring device of the motor axis, so that it is designed for detecting and evaluating a rotational speed / flow rate characteristic. Alternatively, it may also evaluate a rotational speed / power consumption characteristic.
  • the pump function monitoring device has at least one pressure measuring device.
  • it is designed to detect and evaluate a power consumption / pressure characteristic and / or a rotational speed / pressure characteristic. By observing these characteristics, it is possible to check the functionality of the micropump. Since the flow rate measuring device and the pressure measuring device are both part of the biosensor device, no additional sensors are needed.
  • a combination of the characteristics, for example, rotational speed against flow and pressure for better monitoring of the pump can be used.
  • the flow meter generates information about the flow rate (volume per time) of the process fluid.
  • the value of the flow meter must be 0. If this is not the case, the flow measuring device does not work correctly. Possible errors can be a damaged cable or a missing power supply.
  • the flow changes its value depending on the following parameters: rotational speed of the pump, power consumption of the pump, viscosity of the process fluid at a predetermined pump rotational speed, pressure in the system at a given pump rotational speed.
  • the functionality test of the flow measuring device is advantageously designed such that the dependence of one of the mentioned parameters with the signal of the flow measuring device is set in dependence, ie, for example, the rotational speed of the pump relative to the flow rate.
  • the functionality of the flow measuring device can be determined in this way. A combination of the mentioned test values is advantageous.
  • the detection device has at least one inlet channel, by means of which advantageously the process fluids, ie the detection and / or cleaning fluids, can be introduced into the detection device.
  • the detection device has two inlet channels for this purpose.
  • at least one outlet channel is provided at the detection device, in particular a plurality, eg four outlet channels are provided.
  • a selection valve for connecting the at least one outlet channel to the at least one inlet channel is provided as the detector element or as one of a plurality of detector elements;
  • a multi-way selector valve in particular a four-way double selection valve is provided, which is designed to connect the plurality, eg two, inlet channels to the plurality, eg four, outlet channels.
  • the selection valve eg, the four-way dual-port valve, is preferably a USB device and causes a software error when it is not operational or when it has no power supply.
  • the selection valve must sequentially successfully connect to all (eg four) exhaust ports and all (eg two) intake ports.
  • a position identification device is preferably provided as a test device or as one of a plurality of test devices. It can identify the position of the selection valve, eg the four-way double selection valve.
  • a filter is preferably provided which filters out the biological particles from the sample for the purpose of detection.
  • the biological particles are immobilized and provided for detection.
  • the self-test device has a filter function monitoring device which monitors the filter.
  • the self-test device advantageously has at least one differential pressure device which measures the differential pressure in front of and behind an element to be monitored, such as the filter.
  • the position of the selection valve can advantageously be identified via the differential pressure measuring device.
  • the differential pressure measuring device preferably has at least one first pressure sensor which is designed to measure a pressure P1 above the filter, more preferably the differential pressure measuring device has two pressure sensors, wherein the second pressure sensor is designed to measure a second pressure P2 over the filter. It is the Pressure sensor P1 arranged in the fluidic system in front of the filter and the pressure sensor P2 in the fluidic system after the filter.
  • the position identification device comprises the pressure P1 of the first pressure sensor and / or the pressure of the second pressure sensor and can advantageously calculate therefrom the differential pressure across the filter and store this differential pressure.
  • the evaluation device is preferably designed for detecting and evaluating a differential pressure / valve position characteristic, it is possible to determine from the pressure difference between the first and the second pressure sensor in which position the selection valve is currently located.
  • the two pressure sensors In order for the two pressure sensors to be located particularly close to the filter, it is advantageous if they are integrated in a detection chamber in which the filter is located.
  • the value of the pressure sensors should be 0. If this is not the case, they will not work correctly. A potential error could be a missing power supply or a faulty cable.
  • different pressures may be applied to the two pressure sensors.
  • the functionality of the pressure sensors can be monitored by comparing the values while changing the position of the selection valve and pumping fluids through the biosensor device. It is also possible to record the flow rate as a function of the pressure, or to set it with different viscosities and selection valve positions depending on. The results show if the pressure sensors are working properly.
  • At least one optical detection device is provided as the detector element or as one of a plurality of detector elements.
  • This preferably has a light source, in particular a light emitting diode (LED).
  • LED light emitting diode
  • a possible fault of the light emitting diode may be due to a missing power supply or a defective cable. Therefore, as a test device or as one of a plurality of test devices, it is preferable to provide a light function monitor for testing the light source on function.
  • This light-function monitoring device advantageously has an energy meter that measures the energy consumption of the light source. This can be used to monitor whether the LED is working electrically. If the LED is off or defective, its energy consumption is zero. If the LED is working, the energy consumption must be within the range specified by the manufacturer. However, it is also of interest how the light characteristics of the LED are. Therefore, the light-function monitoring device advantageously has at least one light-detecting device, which is configured in particular as a photomultiplier (PMT).
  • PMT photomultiplier
  • the light-function monitoring device is advantageously designed such that it sets the energy consumption of the light source with a test signal of the light-detecting device in dependence and / or can store the determined data.
  • a self-test e.g. compared the detected by the light detection device test signals when the LED is turned on and off. If the LED is on, the light detector should be illuminated, if it is off, it should be in the dark. These values of the light signals can be used to determine the functionality of the LED. If the determined data are stored and thus a history is created, the sensitivity properties of the light detection device can also be observed. This information can be further used for other self-tests or for statistical evaluations.
  • An error of the light detecting device in particular of the photomultiplier, may be a missing power supply or a broken cable. Its functionality is tested in the same way as the functionality of the LED.
  • the biosensor device has at least one sample receiving device, with which samples can be taken from drinking water pipes.
  • this sample receiving device advantageously has at least one safety device.
  • This safety device is preferably a check valve. This prevents that already taken drinking water can return to the drinking water pipe and thus pollutes the drinking water with possibly already used process fluids.
  • the self-test device has at least one temperature monitoring device.
  • the temperature in the biosensor device can be monitored. It is particularly advantageous because the detection of biological articles in only a fairly narrow temperature range is feasible. If the temperatures are too low or too high, the functional groups on which the probes attach to the marking can degenerate and no longer exist. Then a detection of the biological particles is no longer possible.
  • a temperature regulating means which regulates the temperature in the biosensor device. If the temperature monitoring device measures too low a temperature in the biosensor device, it is advantageous if the temperature regulating device has a heating device which heats the biosensor device to the desired temperature. If, on the other hand, the biosensor device has too high a temperature, it is advantageous if the temperature regulating device also has a cooling device which cools the biosensor device to the desired temperature.
  • the self-test device has a leak detection device.
  • damage in the fluidic device can be detected directly, and the necessary repair can be indicated by a signal.
  • the leak detection device has a conductivity device. By measuring the conductivity of the exiting fluids, it is possible to detect exactly where a leak is located.
  • the overall fluidic device may have two possible failures, either it has a leak or it is clogged. If it has a leak, fluid will escape, which can be detected by the leak detection device.
  • a blockage on the other hand, can, depending on the position of the blockage in the Fluidic device, are recognized by the self-tests and the comparison of all self-tests of the individual components of the biosensor device.
  • the self-test device preferably has a data acquisition, in particular electronic data acquisition, which records all data and test signals that are regenerated in the self-test.
  • Data acquisition is the heart of electronic signal processing. It preferably collects all the information of the individual components and delivers them to a process unit (CPU).
  • the data acquisition is preferably a USB device and will cause a software error if it is not ready or if power is not available during initialization. If the data acquisition is not working properly, the biosensor device will not be ready.
  • the biosensor device Before commissioning the septum, a self-test run is performed. After all the test signals have been collected in a self-test matrix, the biosensor device is capable of self-detecting blockage in the fluidic device as well as the position of the blockage.
  • FIG. 1 shows an embodiment of a biosensor device indicated generally by reference numeral 10.
  • the biosensor device 10 has a fluidic device 12 and a detection device 14.
  • the fluidic device 12 is provided for transporting fluids such as detection fluids and / or cleaning fluids and / or a fluid sample through the biosensor device 10.
  • biological particles present in the sample are detected.
  • a sample is first taken from a line 16.
  • a check valve 18 prevents the already taken sample from flowing back into the conduit, thus contaminating the contents of the conduit.
  • the sample is pumped by a pump 20 via a here in the form of a multi-way valve, namely a eight-way valve 22 first selector valve in the fluidic device 12 to the detection device 4.
  • the eight-way valve 22 is further configured to select one of seven vessels 24. In these vessels detection and / or cleaning fluids are stored. Depending on which of the fluids is needed, the eight-way valve 22 selects the respective vessel 24, and since the eight-way valve 22 is connected to the pump 20, the selected fluid in the fluidic device 12 can also be pumped towards the detection device 14.
  • a flow measuring device 26 is provided in the fluidic device 12. With this flow measuring device 26, it is possible to determine which sample volume or volume of the detection fluid has been pumped to the detection device 14.
  • an air bubble sensor 28 is further provided which detects the presence of air bubbles in the fluidic device 12 and outputs a message to remove these bubbles as they interfere with a quantitative analysis of the sample.
  • the sample and / or the detection and / or cleaning fluids to a second selection valve, which is advantageously designed as a multi-way valve and is formed here by a four-way double selection valve 30, pumped.
  • the fluids can take different routes.
  • You can take the path to a filter 38 via a differential pressure measuring device 32 having two pressure sensors 34, 36.
  • the filter 38 is a micromechanical filter, which is used for the detection of particles, in particular of bacteria.
  • This microfilter has or consists of a thin, for example, 1 ⁇ thick membrane, for example of monocrystalline silicon or diamond or has a diamond layer.
  • the membrane is perforated, the holes have a diameter of eg 450 nm, ie the membrane or the microfilter forms a sieve. If, for example, a water sample is pumped through the filter, bacteria of, for example, about 1 ⁇ m in diameter are retained on the filter surface, where they can subsequently be detected.
  • an optical detection device 40 is provided in the detection device 4, which has a light source 42 and a light detection device 44.
  • the particles immobilized on the filter 38 are marked.
  • the light source 42 illuminates the marking probes, they are activated and emit photons in accordance with a fluorescence process during excitation decay. These photons are emitted by the light detector 44, e.g. a photomultiplier is numbered.
  • the fluids may also make their way to a waste 46 in the four-way dual port valve 30.
  • the biosensor device 10 also has a temperature regulating device 48. This comprises a heating device 50 and / or a cooling device 52, which can heat or cool the fluids.
  • a data acquisition 54 is provided in the biosensor device 10.
  • the biosensor device 10 In order to monitor whether the individual components of the biosensor device 10 function correctly, the biosensor device 10 has a self-test function. device 56 on. This is designed for testing the various elements of the fluidic device 12 and / or detection device 14. It has an evaluation device 58, which can evaluate the various test signals. All components of the biosensor device 10 are designed as USB components, and connected via USB connections to the evaluation device 58.
  • the evaluation device 58 is formed by a software 60 processed in a data processing system, which is initialized before each sampling. In this initialization step, all components are tested for functionality. If the self-test device 56 detects a malfunction on one of the components, the biosensor device 10 is switched off.
  • the self-timer 56 is able to detect whether the eight-way valve 22 has selected the correct vessel 24.
  • a power measuring device 64 and a rotational speed measuring device 66 are provided on the pump. From the power and the rotational speed of the pump 20, the viscosity of the pumped fluid can be determined. Since each detection and / or cleaning fluid has a very characteristic viscosity, then it can be recognized from which vessel 24 was selected by the eight-way valve 22.
  • a conductivity meter 68 additionally measures the conductivity of the detection and / or cleaning fluid and can identify it with it.
  • a pH-value measuring device 70 which measures the pH value of the detection and / or cleaning fluid. Due to the characteristic pH values of the fluids can also be identified so which vessel 24 is currently selected.
  • An optical detection unit 72 additionally determines the optical density of the pumped fluid, and can thus identify it.
  • a pressure measuring device 74 and / or a fill level measuring device 76 is provided in each vessel 24, which measures the pressure or the level in the vessel 24. Now, if the corresponding vessel 24 is selected by the eight-way valve 22, and the pump 20 pumps the fluid out of the vessel 24, the value of the pressure measuring device 74 and the fill level measuring device 76 changes a subsequent removal by the pump 20 are closed on which of the vessels 24 is currently selected by the eight-way valve 22.
  • a pump function monitoring device 78 Via various measuring devices, which form a pump function monitoring device 78, the pump 20 is checked for its function.
  • a flow measuring device 80 In addition to the power measuring device 64, a flow measuring device 80, the aforementioned rotational speed measuring device 66 and a pressure measuring device 82 are also provided. These measurement devices pass their test signals to the pump function monitor 78, which is connected to the self-test device 56. In the self-test device 56, various characteristics are determined from the test signals via the evaluation device 58, and thus it is concluded whether the pump 20 is functioning. At the same time it can also be concluded from these data whether the flow measuring device 80 is functional. At a high rotational speed of the pump 20, a high flow rate should also be measured. If this is not the case, the flow measuring device 80 does not work correctly.
  • a position identification device 84 This can be done in the measurement of the differential pressure via the filter 38, which simultaneously acts as a filter function monitoring device 86.
  • test method for identifying the position of the four-way dual selection valve 30 is as follows: Four possible transport routes for the fluids are possible:
  • the fluid is passed directly into the waste 46.
  • the fluid is passed via the first pressure sensor 34 (P1) into a detection chamber 88 having the filter 38 and differential pressure measuring device 32, then flows via the filter 38, and finally into the waste 46 via the second pressure sensor 36 (P2).
  • the fluid is passed via the first pressure sensor 34 (P1) in the detection chamber 88, flows tangentially through the filter 38 and then into the waste 46th
  • the fluid is passed via the second pressure sensor 36 (P2) into the detection chamber 88, flows through the filter 38 in the opposite direction to 2 and then via the first pressure sensor 34 (P1) in the waste 46th
  • the correct position information of the four-way dual selection valve 30 can be identified.
  • the functionality of the first pressure sensor 34 and the second pressure sensor 36 can be checked via such a pressure comparison.
  • the light source 42 is monitored by a light function monitoring device 90 for their functionality. This is done on the one hand via an energy metering device 92, which indicates whether the light source 42 works in principle. At the same time, the light-detecting device 44 outputs data on how much it is illuminated. It can be deduced from these data how many photons the light source 42 emits. It can be concluded that the functionality of the light source 42. Conversely, of course, the light detection device 44 can be checked from the same time.
  • the self-test device 56 also includes a temperature monitor 94. Furthermore, a leak detection device 96 is provided which detects whether there are defects in the fluidic device 12 at which the fluids escape.
  • the fluidic device 12 as a whole can be tested for functionality by comparing and using all other data generated on all other components. From this it can be concluded whether and at which point the fluidic device 12 may possibly have a blockage.
  • the following two tables show two examples of how to detect a clogged fluidic device 12, and thereby identify the position of clogging.
  • Table 1 Case where the fluidic device 12 between the detection chamber 88 and the waste 46 is clogged.
  • Table 2 Case when the fluidic device 12 between the four-way dual selection valve 30 and the detection chamber 88 is clogged. Device test status
  • Dual selection valve 30 channel / pressure measurement error

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Abstract

L'invention concerne un dispositif de biodétection (10) qui présente un système de test automatique (56) et est configuré pour identifier les fonctions d'erreur des différents éléments constitutifs du dispositif de biodétection (10). Si le système de test automatique (56) détecte une erreur concernant un des éléments constitutifs du dispositif de biodétection (10), il met le dispositif de biodétection (10) hors tension pour des raisons de sécurité.
PCT/EP2010/062480 2009-10-08 2010-08-26 Dispositif de biodétection équipé d'un système de test automatique WO2011042253A1 (fr)

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CN113317265B (zh) * 2021-05-28 2022-05-20 重庆工商大学 一种循环水养殖智能控制系统

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