WO2011027719A1 - Proliferation-enhancing agent and/or survivability-improving agent for lactic acid bacterium belonging to genus lactobacillus - Google Patents
Proliferation-enhancing agent and/or survivability-improving agent for lactic acid bacterium belonging to genus lactobacillus Download PDFInfo
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- WO2011027719A1 WO2011027719A1 PCT/JP2010/064569 JP2010064569W WO2011027719A1 WO 2011027719 A1 WO2011027719 A1 WO 2011027719A1 JP 2010064569 W JP2010064569 W JP 2010064569W WO 2011027719 A1 WO2011027719 A1 WO 2011027719A1
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- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A23L33/17—Amino acids, peptides or proteins
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- the present invention relates to a growth promoter and / or survival improver for Lactobacillus lactic acid bacteria, which contains a ⁇ -caseinoglyco macropeptide-containing substance as an active ingredient.
- lactic acid bacteria Useful microorganisms that can have beneficial effects on the host, such as improving the bacterial flora in the gastrointestinal tract, are called probiotics and are attracting attention.
- One such useful microorganism is lactic acid bacteria.
- lactic acid bacteria Recently, research on functionality of lactic acid bacteria has become active, and development of products incorporating new functionality using the lactic acid bacteria has also been progressing. Examples of such lactic acid bacteria include lactic acid bacteria belonging to the genus Lactobacillus.
- Fermented foods typified by fermented milk have a low pH environment and are often not suitable for the growth and survival of lactic acid bacteria. Promoting the growth of lactic acid bacteria in food or improving the survival is a great contribution to preventive medicine when the lactic acid bacteria have the functionality of probiotics as described above.
- Japanese Patent No. 3447358 includes a specific amount of lipid obtained by subjecting milk or a milk material (eg, buttersarum, buttermilk) to a specific treatment as a “lactic acid bacteria survival improver”. What to do is disclosed. Specifically, it is disclosed that this improver is added to a medium of lactic acid bacteria.
- Japanese Patent Application Laid-Open No. 2007-097447 discloses a bifidobacteria characterized by using a culture, a solvent extract and / or a treated product of a propionic acid bacterium and / or a compound-producing bacterium having a naphthoquinone ring.
- the survival improvement method of this is disclosed.
- propionic acid bacteria produce a growth promoting substance of bifidobacteria.
- ⁇ -caseinoglyco macropeptide has an effect of promoting the growth of bifidobacteria, but its mechanism of action has not yet been elucidated. Furthermore, as far as the present inventors know, it has not been known at all that ⁇ -caseinoglyco macropeptide has an effect of promoting the growth of lactic acid bacteria and an effect of improving the survival.
- the present inventors have recently promoted the growth of the lactic acid bacteria contained in the food composition without affecting the flavor and physical properties of the food composition containing the Lactobacillus lactic acid bacteria, and the survival properties thereof.
- the method to improve the effect was examined experimentally.
- ⁇ -caseinoglyco macropeptide which is a milk-derived material, without affecting the flavor and physical properties of the food composition containing Lactobacillus lactic acid bacteria, promote the growth of the lactic acid bacteria, And succeeded in improving the survival.
- the sustainability of the effect is high and excellent, and it was found that the quality can be maintained even during storage of the food composition.
- the present invention is based on these findings.
- the object is to provide a growth promoter and / or survival improver for Lactobacillus lactic acid bacteria that is excellent in sustainability and can contribute to quality maintenance during food storage.
- a growth promoter and / or survival improver for Lactobacillus lactic acid bacteria comprising ⁇ -caseinoglyco macropeptide as an active ingredient
- [4] The Lactobacillus lactic acid bacteria growth promoter and / or survival improver according to any one of [1] to [3] is added to the Lactobacillus lactic acid bacteria-containing composition.
- a method for promoting growth and / or improving survival of Lactobacillus lactic acid bacteria in a Lactobacillus lactic acid bacteria-containing composition [5] Before the fermentation of the lactic acid bacteria-containing composition, the lactic acid bacteria growth promoter and / or survival improver is added to the lactic acid bacteria-containing composition, [4] The method described in [6] A food composition comprising the Lactobacillus lactic acid bacteria growth promoter and / or survival improver according to any one of [1] to [3], and Lactobacillus lactic acid bacteria, [7] Use of ⁇ -caseinoglyco macropeptide for promoting growth and / or improving survival of Lactobacillus lactic acid bacteria in a composition containing Lactobacillus lactic acid bacteria.
- the composition containing Lactobacillus lactic acid bacteria such as food and food composition can be obtained by using ⁇ -caseinoglyco macropeptide.
- the growth of Lactobacillus lactic acid bacteria can be promoted and / or the survival can be improved.
- the growth of Lactobacillus lactic acid bacteria can be promoted and / or the survival can be improved.
- the growth of Lactobacillus genus lactic acid bacteria is promoted and / or survivability.
- the growth of Lactobacillus lactic acid bacteria can be promoted and / or the survival can be improved.
- the use of ⁇ -caseinoglyco macropeptide promotes the growth of the lactic acid bacteria without affecting the flavor and physical properties of foods and food compositions containing Lactobacillus lactic acid bacteria. / Or survival can be improved.
- 6 is a graph showing the survival rate of the number of bacteria of Lactobacillus gasseri OLL2716 strain (number of viable cells after storage days / number of viable cells at the start of storage (%)) in Experimental Example 1.
- 6 is a graph showing the survival rate of the number of bacteria of Lactobacillus gasseri OLL2959 strain in Experimental Example 2 (number of viable cells after storage days / number of viable cells at the start of storage (%)).
- 10 is a graph showing the number of bacteria (cfu / g) of Lactobacillus gasseri OLL2716 strain in Experimental Example 3.
- Lactic acid bacteria growth promoter and / or survival improver contains ⁇ -caseinoglyco macropeptide as an active ingredient. That is, the present invention promotes the growth of probiotic bacteria such as Lactobacillus lactic acid bacteria contained in yogurt and / or improves survival by using ⁇ -caseinoglyco macropeptide.
- probiotic bacteria such as Lactobacillus lactic acid bacteria contained in yogurt
- lactic acid bacterium growth promoter and / or survival enhancer refers to a lactic acid bacterium growth promoter, a lactic acid bacterium survival promoter, in addition to a drug that has both the performance of lactic acid bacteria growth promotion and survival improvement.
- lactic acid bacteria growth promoter and / or survival improver is an agent for promoting the growth and survival of lactic acid bacteria, or for promoting the growth and survival of lactic acid bacteria. It can be paraphrased as an additive for promoting the growth of lactic acid bacteria and improving survival.
- proliferation promotion of lactic acid bacteria means that when the lactic acid bacteria are in an environment suitable for the growth or maintenance of the lactic acid bacteria, the lactic acid bacteria act on the lactic acid bacteria to promote the growth of the lactic acid bacteria, thereby increasing the number of bacteria. Say that. In the present invention, preferably, it means that growth of lactic acid bacteria is promoted in a composition containing lactic acid bacteria under a predetermined storage environment.
- “improvement of survival” of lactic acid bacteria means that the number of lactic acid bacteria remaining alive by acting on lactic acid bacteria to suppress the death of lactic acid bacteria when in an environment suitable for the growth or maintenance of lactic acid bacteria. Say to maintain and improve.
- the number of surviving lactic acid bacteria is improved under the predetermined storage environment.
- ⁇ -caseinoglyco macropeptide is a fragment of ⁇ -casein, which is one of milk proteins, and is a C-terminal peptide containing a glycopeptide of ⁇ -casein.
- the ⁇ -caseinoglyco macropeptide is usually part of the casein micelle and is located at the outer edge of the micelle.
- the ⁇ -caseinoglyco macropeptide is usually available in the form of a ⁇ -caseinoglyco macropeptide-containing product.
- the ⁇ -caseinoglyco macropeptide-containing product can be prepared by separation by various methods. For example, it can be prepared by taking chymosin into milk protein.
- rennet, pepsin, or both can act on acid casein for curdling to form a rennet casein card, and can be prepared by utilizing whey produced at that time.
- the whey contains calcium phosphate, and the pH and temperature are adjusted (for example, the pH is adjusted to 8 to 9, and the temperature is adjusted to 50 to 60 ° C.) to precipitate and remove the calcium phosphate.
- the supernatant obtained in this way is adjusted to return to the neutral region, and after concentration and desalting with a reverse osmosis membrane, it can be prepared by spray drying or the like, if necessary.
- ⁇ -caseinoglyco macropeptide may be used if it is commercially available.
- the Lactobacillus lactic acid bacteria growth promoter and / or survival improver of the present invention includes the above-mentioned ⁇ -caseinoglyco macropeptide-containing product or a treated fraction as it is or diluted with a solvent. It is obtained as an insoluble fraction and can be used as it is.
- a solvent water or a commonly used solvent, for example, alcohols, hydrocarbons, organic acids, organic bases, inorganic acids, inorganic bases, supercritical fluids and the like can be used alone or in combination.
- Lactobacillus lactic acid bacteria growth promoter and / or survival improver of the present invention includes the above-mentioned ⁇ -caseinoglyco macropeptide itself or a processed product thereof, such as water, protein, carbohydrate, lipid, vitamin. It is also possible to obtain a combination of food compositions such as foods, minerals, organic acids, organic bases, fruit juices and flavors.
- the growth promoter and / or survival improver of lactic acid bacteria of the present invention is preferably 0.05 to 0.6% by weight, more preferably 0.1% in terms of the concentration of ⁇ -caseinoglyco macropeptide. It can be added to the lactic acid bacterium-containing composition so as to contain ⁇ 0.4 wt%, more preferably 0.2 ⁇ 0.3 wt%. At this time, the lactic acid bacterium-containing composition preferably contains Lactobacillus lactic acid bacteria, preferably 10 5 CFU / g or more, more preferably in the range of 10 6 to 10 9 CFU / g, and still more preferably 10 7 to 10 8 CFU. / G.
- the growth promoter and / or survival improver of Lactobacillus lactic acid bacteria promotes the growth and / or survival of the lactic acid bacteria in a low pH environment. It was able to demonstrate the function to improve the.
- Foods and food compositions containing lactic acid bacteria, such as fermented milk, are generally in a low pH environment and are often not suitable for the growth and / or survival of lactic acid bacteria.
- the growth promoter and / or survival improver of the present invention can exhibit functions of promoting the growth of lactic acid bacteria and / or improving the survival even in a low pH environment
- the lactic acid bacteria represented by fermented milk and the like It is useful in promoting the growth of lactic acid bacteria and / or improving the survival in foods and food compositions containing sucrose.
- the lactic acid bacteria growth promoter and / or survival improver of the present invention is more effective in a low pH environment that is differentiated from the prior art in consideration of the pH of foods and food compositions containing lactic acid bacteria. Can demonstrate its function.
- the low pH environment is preferably 2.5 to 6.5, more preferably 3.0 to 5.5, still more preferably 3.5 to 5.0, and most preferably pH.
- the pH is 3.8 to 4.8.
- the Lactobacillus lactic acid bacteria growth promoter and / or survival improver according to the present invention is particularly applied to Lactobacillus gasseri species whose excellent efficacy is being elucidated as Lactobacillus lactic acid bacteria. Is preferred.
- the Lactobacillus gasseri species are, for example, Lactobacillus gasseri OLL2716 (Lactobacillus gasseri OLL2716: FERM BP-6999) strain, Lactobacillus gasseri OLL2959 strain (Lactobacillus gasseri OLL2959: N).
- the effects of the lactic acid bacteria growth promoter and / or survival improver of the present invention have been experimentally confirmed in these strains.
- Lactobacillus gasseri OLL2716 strain is deposited at the National Institute of Advanced Industrial Science and Technology Patent Biological Deposit Center. The contents specifying the deposit will be described below.
- the above Lactobacillus gasseri OLL2716 was isolated from human feces.
- Lactobacillus gasseri OLL2959 strain is deposited at the Patent Microorganism Depositary Center for Product Evaluation Technology Foundation. The contents specifying the deposit will be described below.
- the above Lactobacillus gasseri OLL2959 strain was isolated from human feces. It is a gram-positive rod and does not produce gas from glucose. Utilizes glucose, mannose, fructose, galactose, sucrose, cellobiose.
- the method for promoting the growth and / or improving the survival of the Lactobacillus lactic acid bacterium comprises the above-mentioned ⁇ -caseinoglyco macropeptide-containing product as an active ingredient and a Lactobacillus lactic acid bacterium growth promoter and / or survival. It is performed by adding a property improver to a Lactobacillus genus lactic acid bacteria containing composition.
- the timing of the addition may be any before, during, or after fermentation of the Lactobacillus lactic acid bacteria-containing composition, but preferably contains the lactic acid bacteria because it is easy to uniformly manage the effects and actions of the entire product. It is before fermentation of the composition, and more preferably before sterilization of the lactic acid bacteria-containing composition, since the ⁇ -caseinoglyco macropeptide-containing material can be easily managed hygienically.
- the growth promoter and / or survival improver of the present invention may further be used in foods and food compositions. That is, the growth promoter and / or survival improver of the present invention can be used for foods and food compositions containing the lactic acid bacteria.
- the food and food composition include various foods and beverages (soft drinks, fermented milk, yogurt, etc.).
- a food or a food composition containing the growth promoter and / or survival improver of the present invention and the lactic acid bacterium described above can be used as it is or mixed with other foods or food ingredients. It can be used according to conventional methods in the composition. In addition, the state and form of food and drink that are usually used, such as solid (powder, granule, etc.), paste, liquid, or suspension may be used.
- ingredients are not particularly limited, but water, protein, carbohydrates, lipids, vitamins, minerals, organic acids, organic bases, fruit juices, flavors, etc. are used as ingredients in the foods and food compositions. be able to. These components can be used in combination of two or more kinds, and synthetic products and / or foods and food compositions containing many of these may be used.
- Lactobacillus gasseri OLL2716 strain (FERM BP-6999), a probiotic strain of yogurt, and Lactobacillus gasseri OLL2959 strain (NITE BP-244), a probiotic strain of the same strain, in the yogurt -The effect of caseinoglyco macropeptide (CGMP) was examined.
- CGMP caseinoglyco macropeptide
- CGMP-10 manufactured by Arraughs Ingredients Japan Co., Ltd. was used.
- yogurts were stored at 5 ° C. for 16 days as the expiration date. During the storage period, there was no difference in flavor, physical properties, lactic acid acidity, and pH depending on whether or not CGMP was added. Furthermore, at the start of storage (immediately after preparation), 8 days and 16 days later, the number of bacteria of Lactobacillus gasseri OLL2716 strain was measured. Table 1 shows the number of bacteria and the survival rate (the number of viable bacteria after the storage days / the number of viable bacteria at the start of storage (%)). Further, the survival rate of the number of bacteria of the Lactobacillus gasseri OLL2716 strain (number of viable cells after storage days / number of viable cells at the start of storage (%)) is shown in FIG.
- yogurts were stored at 5 ° C. for 16 days as the expiration date. During the storage period, there was no difference in flavor, physical properties, lactic acid acidity, and pH depending on whether or not CGMP was added. Furthermore, at the start of storage (immediately after preparation), 8 days and 16 days later, the number of bacteria of Lactobacillus gasseri OLL2959 strain was measured. Table 2 shows the number of bacteria and the survival rate (the number of viable cells after the storage days / the number of viable cells at the start of storage (%)). Further, the survival rate of the number of bacteria of the Lactobacillus gasseri OLL2959 strain (number of viable cells after storage days / number of viable cells at the start of storage (%)) is shown in FIG.
- the survival rate of Lactobacillus gasseri OLL2716 strain was 100% at the start of storage (immediately after preparation), 22.1% when CGMP was not added, and CGMP In the case of addition, it was 42.8%.
- the survival rate of Lactobacillus gasseri OLL2959 strain is 100% at the start of storage (immediately after preparation), 20.6% when CGMP is not added, and CGMP is added In this case, it was 41.9%.
- the survival rate of these lactic acid bacteria is very important in ensuring the quality of the product, and in the present invention, the element can be remarkably improved. Here, the survival improvement effect of lactic acid bacteria was confirmed.
- Lactobacillus gasseri OLL2716 strain (FERM BP-6999), a probiotic strain of yogurt, was used to examine the effect of ⁇ -caseinoglyco macropeptide (CGMP) on growth promotion.
- CGMP ⁇ -caseinoglyco macropeptide
- ⁇ -caseinoglyco macropeptide-containing material By using the ⁇ -caseinoglyco macropeptide-containing material according to the present invention, there is no influence on the flavor and physical properties of the food composition, and the effect is highly durable and excellent. Alternatively, a survival improver can be provided.
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Abstract
Description
本発明は、κ-カゼイノグリコマクロペプチド含有物を有効成分とする、ラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤に関する。 TECHNICAL FIELD The present invention relates to a growth promoter and / or survival improver for Lactobacillus lactic acid bacteria, which contains a κ-caseinoglyco macropeptide-containing substance as an active ingredient.
消化管内の細菌叢を改善するなど、宿主に有益な作用をもたらしうる有用な微生物は、プロバイオティクスと称され注目を集めている。このような有用な微生物の一つとして、乳酸菌がある。最近では、乳酸菌の機能性研究が盛んになってきており、その乳酸菌を利用して、新規な機能性を盛り込んだ商品の開発も進んでいる。そのような乳酸菌として、ラクトバチルス(Lactobacillus)属乳酸菌等が挙げられる。 Related Art Useful microorganisms that can have beneficial effects on the host, such as improving the bacterial flora in the gastrointestinal tract, are called probiotics and are attracting attention. One such useful microorganism is lactic acid bacteria. Recently, research on functionality of lactic acid bacteria has become active, and development of products incorporating new functionality using the lactic acid bacteria has also been progressing. Examples of such lactic acid bacteria include lactic acid bacteria belonging to the genus Lactobacillus.
[1] κ-カゼイノグリコマクロペプチドを有効成分として含む、ラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤、
[2] ラクトバチルス属乳酸菌がラクトバチルス・ガセリ(Lactobacillus gasseri)種である、前記[1]に記載のラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤、
[3] ラクトバチルス属乳酸菌が、Lactobacillus gasseri OLL2716株、及びLactobacillus gasseri OLL2959株から選択される1種以上のものである、前記[1]または[2]に記載のラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤、
[4] 前記[1]~[3]のいずれかに記載のラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤を、ラクトバチルス属乳酸菌含有組成物に添加することを特徴とする、ラクトバチルス属乳酸菌含有組成物においてラクトバチルス属乳酸菌を増殖促進及び/又は生残性向上させる方法、
[5] 前記の乳酸菌含有組成物の発酵前に、前記の乳酸菌の増殖促進剤及び/又は生残性向上剤を、前記の乳酸菌含有組成物に添加することを含んでなる、前記[4]に記載の方法、
[6] 前記[1]~[3]のいずれかに記載のラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤と、ラクトバチルス属乳酸菌とを含んでなる、食品組成物、
[7] ラクトバチルス属乳酸菌含有組成物において、ラクトバチルス属乳酸菌を増殖促進及び/又は生残性向上させるための、κ-カゼイノグリコマクロペプチドの使用。 That is, according to the present invention, the following inventions are provided.
[1] A growth promoter and / or survival improver for Lactobacillus lactic acid bacteria, comprising κ-caseinoglyco macropeptide as an active ingredient,
[2] The Lactobacillus lactic acid bacteria growth promoter and / or survival improver according to [1], wherein the Lactobacillus lactic acid bacteria is Lactobacillus gasseri species,
[3] The Lactobacillus lactic acid bacteria growth promoter according to [1] or [2], wherein the Lactobacillus lactic acid bacteria is one or more selected from Lactobacillus gasseri OLL2716 strain and Lactobacillus gasseri OLL2959 strain And / or survival improver,
[4] The Lactobacillus lactic acid bacteria growth promoter and / or survival improver according to any one of [1] to [3] is added to the Lactobacillus lactic acid bacteria-containing composition. A method for promoting growth and / or improving survival of Lactobacillus lactic acid bacteria in a Lactobacillus lactic acid bacteria-containing composition,
[5] Before the fermentation of the lactic acid bacteria-containing composition, the lactic acid bacteria growth promoter and / or survival improver is added to the lactic acid bacteria-containing composition, [4] The method described in
[6] A food composition comprising the Lactobacillus lactic acid bacteria growth promoter and / or survival improver according to any one of [1] to [3], and Lactobacillus lactic acid bacteria,
[7] Use of κ-caseinoglyco macropeptide for promoting growth and / or improving survival of Lactobacillus lactic acid bacteria in a composition containing Lactobacillus lactic acid bacteria.
本発明による乳酸菌の増殖促進剤及び/又は生残性向上剤は、κ-カゼイノグリコマクロペプチドを有効成分として含むものである。すなわち、本発明は、κ-カゼイノグリコマクロペプチドを使用することで、ヨーグルトなどに含まれるラクトバチルス属乳酸菌などのプロバイオティクス菌の増殖を促進及び/又は生残性を向上させるものである。
ここで、「乳酸菌の増殖促進剤及び/又は生残性向上剤」とは、乳酸菌の増殖促進及び生残性向上の両方の性能を含む薬剤に加えて、乳酸菌の増殖促進、乳酸菌の生残性向上の一方のみの性能を奏する薬剤を包含する意味で使用される。ただし、これらの意味を包含する表現として、「乳酸菌の増殖促進剤及び/又は生残性向上剤」は、乳酸菌の増殖促進及び生残性向上剤、又は乳酸菌の増殖促進及び生残性向上用組成物、又は乳酸菌の増殖促進及び生残性向上用添加剤と言い換えることもできる。 Lactic acid bacteria growth promoter and / or survival improver The lactic acid bacteria growth promoter and / or survival improver according to the present invention contains κ-caseinoglyco macropeptide as an active ingredient. That is, the present invention promotes the growth of probiotic bacteria such as Lactobacillus lactic acid bacteria contained in yogurt and / or improves survival by using κ-caseinoglyco macropeptide. .
Here, the term “lactic acid bacterium growth promoter and / or survival enhancer” refers to a lactic acid bacterium growth promoter, a lactic acid bacterium survival promoter, in addition to a drug that has both the performance of lactic acid bacteria growth promotion and survival improvement. It is used in the sense of including a drug that exhibits only one of the performance enhancement properties. However, as an expression including these meanings, “lactic acid bacteria growth promoter and / or survival improver” is an agent for promoting the growth and survival of lactic acid bacteria, or for promoting the growth and survival of lactic acid bacteria. It can be paraphrased as an additive for promoting the growth of lactic acid bacteria and improving survival.
κ-カゼイノグリコマクロペプチド含有物は、種々の方法で分離して調製できる。例えば、乳タンパク質にキモシンを作用させることで取り出して調製できる。また、例えば、κ-カゼインにペプシンを作用させてから酸を加えて、パラκ-カゼイン画分を沈殿させて得られた上清部を透析して脱塩した後に、必要に応じて凍結乾燥などすることで調製できる。さらに、例えば、レンネットやペプシンあるいは両者を酸カゼインに作用させて凝乳処理し、レンネットカゼインカードを形成させて、その際に生成するホエイを利用することで調製できる。具体的には、該ホエイでは、リン酸カルシウムを含んでおり、pHと温度を調整して(例えば、pHを8~9とし、温度を50~60℃に調整して)、リン酸カルシウムを沈殿させて除去して得られる上清部を中性領域に戻して調整してから、逆浸透膜で濃縮して脱塩した後に、必要に応じて噴霧乾燥などすることで調製できる。
あるいは、κ-カゼイノグリコマクロペプチドは、市販品があればそれを利用してもよい。 Here, κ-caseinoglyco macropeptide (CGMP) is a fragment of κ-casein, which is one of milk proteins, and is a C-terminal peptide containing a glycopeptide of κ-casein. The κ-caseinoglyco macropeptide is usually part of the casein micelle and is located at the outer edge of the micelle. The κ-caseinoglyco macropeptide is usually available in the form of a κ-caseinoglyco macropeptide-containing product.
The κ-caseinoglyco macropeptide-containing product can be prepared by separation by various methods. For example, it can be prepared by taking chymosin into milk protein. In addition, for example, after pepsin is allowed to act on κ-casein, acid is added, and the supernatant obtained by precipitating the para κ-casein fraction is dialyzed and desalted, and then lyophilized as necessary. It can prepare by. Furthermore, for example, rennet, pepsin, or both can act on acid casein for curdling to form a rennet casein card, and can be prepared by utilizing whey produced at that time. Specifically, the whey contains calcium phosphate, and the pH and temperature are adjusted (for example, the pH is adjusted to 8 to 9, and the temperature is adjusted to 50 to 60 ° C.) to precipitate and remove the calcium phosphate. The supernatant obtained in this way is adjusted to return to the neutral region, and after concentration and desalting with a reverse osmosis membrane, it can be prepared by spray drying or the like, if necessary.
Alternatively, κ-caseinoglyco macropeptide may be used if it is commercially available.
なお、後述するように、本発明においては、本発明の乳酸菌の増殖促進剤及び/又は生残性向上剤の効果をこれら菌株において実験的に確認している。 The Lactobacillus lactic acid bacteria growth promoter and / or survival improver according to the present invention is particularly applied to Lactobacillus gasseri species whose excellent efficacy is being elucidated as Lactobacillus lactic acid bacteria. Is preferred. The Lactobacillus gasseri species are, for example, Lactobacillus gasseri OLL2716 (Lactobacillus gasseri OLL2716: FERM BP-6999) strain, Lactobacillus gasseri OLL2959 strain (Lactobacillus gasseri OLL2959: N).
In addition, as described later, in the present invention, the effects of the lactic acid bacteria growth promoter and / or survival improver of the present invention have been experimentally confirmed in these strains.
Lactobacillus gasseri OLL2716株は、独立行政法人産業技術総合研究所特許生物寄託センターに寄託されている。該寄託を特定する内容を下記する。
(1)寄託機関名:独立行政法人産業技術総合研究所 特許生物寄託センター
(2)連絡先:〒305-8566 茨城県つくば市東1丁目1番1 中央第6
(3)受託番号:FERM BP-6999
(4)識別のための表示: Lactobacillus gasseri OLL2716
(5)原寄託日:平成11年5月24日
(6)ブタペスト条約に基づく寄託への移管日:平成12年1月14日
上記のLactobacillus gasseri OLL2716は、ヒト糞便より分離された。グラム陽性桿菌であり、グルコースよりガスを産生せず、カタラーゼ陰性を示す。グルコース、マンノース、フルクトース、ガラクトース、シュークロース、セロビオース、ラクトース、トレハロース、スターチ、デキストリンを資化して酸を生成する。 Here, the information regarding the deposit of these lactic acid bacteria is as follows.
The Lactobacillus gasseri OLL2716 strain is deposited at the National Institute of Advanced Industrial Science and Technology Patent Biological Deposit Center. The contents specifying the deposit will be described below.
(1) Name of depositary institution: National Institute of Advanced Industrial Science and Technology, Patent Biological Depositary Center (2) Contact: Chuo 6th 1-1 1-1 Higashi 1-chome Tsukuba City, Ibaraki 305-8565
(3) Accession number: FERM BP-6999
(4) Display for identification: Lactobacillus gasseri OLL2716
(5) Date of original deposit: May 24, 1999 (6) Date of transfer to deposit under the Budapest Treaty: January 14, 2000 The above Lactobacillus gasseri OLL2716 was isolated from human feces. It is a gram positive bacillus, does not produce gas from glucose, and shows catalase negative. Glucose, mannose, fructose, galactose, sucrose, cellobiose, lactose, trehalose, starch, dextrin are assimilated to produce an acid.
(1)寄託機関名:独立行政法人製品評価技術基盤機構特許微生物寄託センター
(2)連絡先:〒292-0818 千葉県木更津市かずさ鎌足2-5-8
(3)受託番号:NITE BP-224
(4)識別のための表示:Lactobacillus gasseri OLL2959
(5)原寄託日:2006年3月31日
(6)ブタペスト条約に基づく寄託への移管日:2007年11月21日
上記のLactobacillus gasseri OLL2959株は、ヒト糞便より分離された。グラム陽性桿菌であり、グルコースよりガスを産生しない。グルコース、マンノース、フルクトース、ガラクトース、シュークロース、セロビオースを資化する。 On the other hand, Lactobacillus gasseri OLL2959 strain is deposited at the Patent Microorganism Depositary Center for Product Evaluation Technology Foundation. The contents specifying the deposit will be described below.
(1) Name of depositary institution: National Institute for Product Evaluation Technology Patent Microorganisms Deposit Center (2) Contact: 2-5-8 Kazusa Kamashichi, Kisarazu City, Chiba Prefecture 292-0818
(3) Accession number: NITE BP-224
(4) Display for identification: Lactobacillus gasseri OLL2959
(5) Date of original deposit: March 31, 2006 (6) Date of transfer to deposit under the Budapest Treaty: November 21, 2007 The above Lactobacillus gasseri OLL2959 strain was isolated from human feces. It is a gram-positive rod and does not produce gas from glucose. Utilizes glucose, mannose, fructose, galactose, sucrose, cellobiose.
CGMPを0.2%で添加したヨーグルトミックスへ、スターター(Lactobacillus bulgaricusとStreptococcus thermophilusの混合培養物)を2%と、Lactobacillus gasseri OLL2716株の濃縮菌液(4×1011 CFU/g)を0.025%で同時に添加して発酵し、ヨーグルトを調製した。一方、CGMPを添加しないこと以外は全く同様に、ヨーグルトを調製し、コントロールとした。 Experimental example 1
To a yogurt mix supplemented with CGMP at 0.2%, 2% of a starter (a mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus) and a concentrated bacterial solution of Lactobacillus gasseri OLL2716 strain (4 × 10 11 CFU / g). A yogurt was prepared by simultaneously adding and fermenting at 025%. On the other hand, yogurt was prepared in the same manner except that CGMP was not added, and used as a control.
CGMPを0.2%で添加したヨーグルトミックスへ、スターター(Lactobacillus bulgaricusとStreptococcus thermophilusの混合培養物)を2%と、Lactobacillus gasseri OLL2959株の濃縮菌液(2×1011 CFU/g)を0.04%で同時に添加して発酵し、ヨーグルトを調製した。一方、CGMPを添加しないこと以外は全く同様に、ヨーグルトを調製し、コントロールとした。 Experimental example 2
To a yogurt mix supplemented with CGMP at 0.2%, a starter (a mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus) 2% and a concentrated bacterial solution of Lactobacillus gasseri OLL2959 strain (2 × 10 11 CFU / g) The yogurt was prepared by simultaneously adding and fermenting at 04%. On the other hand, yogurt was prepared in the same manner except that CGMP was not added, and used as a control.
CGMPを0.2%で添加したMRS培地(Difco Lactobacilli MRS Broth (Becton, Dickinson and Company))に、Lactobacillus gasseri OLL2716株の濃縮菌液(4×1011 CFU/g)を10倍に希釈してから1%で接種し、40℃で培養した。一方、CGMPを添加する代わりに同量の脱脂粉乳を添加した培地を調製して同様の試験をし、コントロールとした。
それぞれ経時的に、それらの菌数を測定した。結果は表3と図3に示されるとおりであった。 Experimental example 3
A concentrated bacterial solution (4 × 10 11 CFU / g) of Lactobacillus gasseri OLL2716 strain was diluted 10-fold in MRS medium (Difco Lactobacilli MRS Broth (Becton, Dickinson and Company)) supplemented with CGMP at 0.2%. To 1% and cultured at 40 ° C. On the other hand, instead of adding CGMP, a medium containing the same amount of skim milk was prepared and subjected to the same test as a control.
Each of them was counted over time. The results were as shown in Table 3 and FIG.
Claims (7)
- κ-カゼイノグリコマクロペプチドを有効成分として含む、ラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤。 Lactobacillus lactic acid bacteria growth promoter and / or survival improver containing κ-caseinoglyco macropeptide as an active ingredient.
- ラクトバチルス属乳酸菌がラクトバチルス・ガセリ(Lactobacillus gasseri)種である、請求項1に記載のラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤。 The growth promoter and / or survival improver of Lactobacillus lactic acid bacteria according to claim 1, wherein the Lactobacillus lactic acid bacterium is Lactobacillus gasseri species.
- ラクトバチルス属乳酸菌が、Lactobacillus gasseri OLL2716株、及びLactobacillus gasseri OLL2959株から選択される1種以上のものである、請求項1または2に記載のラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤。 Lactobacillus lactic acid bacteria are one or more selected from Lactobacillus gasseri OLL2716 strain and Lactobacillus gasseri OLL2959 strain, The growth promoter and / or survival of Lactobacillus lactic acid bacteria according to claim 1 or 2 Improver.
- 請求項1~3のいずれか一項に記載のラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤を、ラクトバチルス属乳酸菌含有組成物に添加することを特徴とする、ラクトバチルス属乳酸菌含有組成物においてラクトバチルス属乳酸菌を増殖促進及び/又は生残性向上させる方法。 Lactobacillus lactic acid bacteria growth promoter and / or survival improver according to any one of claims 1 to 3 is added to a Lactobacillus lactic acid bacteria-containing composition, A method for promoting growth and / or improving survival of Lactobacillus lactic acid bacteria in a composition containing lactic acid bacteria.
- 前記の乳酸菌含有組成物の発酵前に、前記の乳酸菌の増殖促進剤及び/又は生残性向上剤を、前記の乳酸菌含有組成物に添加することを含んでなる、請求項4に記載の方法。 5. The method according to claim 4, comprising adding the lactic acid bacterium growth promoter and / or the survival improver to the lactic acid bacteria-containing composition before fermentation of the lactic acid bacteria-containing composition. .
- 請求項1~3のいずれか一項に記載のラクトバチルス属乳酸菌の増殖促進剤及び/又は生残性向上剤と、ラクトバチルス属乳酸菌とを含んでなる、食品組成物。 A food composition comprising the growth promoter and / or survival improver of Lactobacillus lactic acid bacteria according to any one of claims 1 to 3, and Lactobacillus lactic acid bacteria.
- ラクトバチルス属乳酸菌含有組成物において、ラクトバチルス属乳酸菌を増殖促進及び/又は生残性向上させるための、κ-カゼイノグリコマクロペプチドの使用。 Use of κ-caseinoglyco-macropeptide for promoting growth and / or improving survival of Lactobacillus lactic acid bacteria in a composition containing Lactobacillus lactic acid bacteria.
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JP2011529889A JP5964588B2 (en) | 2009-09-02 | 2010-08-27 | Lactobacillus lactic acid bacteria growth promoter and / or survival improver |
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WO2013136768A1 (en) * | 2012-03-13 | 2013-09-19 | 株式会社明治 | Yogurt containing collagen and method for producing same |
JP2013192470A (en) * | 2012-03-16 | 2013-09-30 | Snow Brand Milk Products Co Ltd | Food and drink containing lactobacillus with high survival rate and method for producing the same |
JP2015050993A (en) * | 2013-08-07 | 2015-03-19 | 国立大学法人 岡山大学 | Gastrointestinal tract and feces-derived lactobacilli culture milk or soybean milk-derived composition, culture or fermentation material and lactic fermentation product |
JP2018201405A (en) * | 2017-06-05 | 2018-12-27 | 雪印メグミルク株式会社 | Composition for survival improvement and/or growth promotion of lactic acid bacteria |
WO2021246392A1 (en) | 2020-06-02 | 2021-12-09 | アサヒグループホールディングス株式会社 | Lactic acid bacteria growth promoter |
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CN106858261A (en) * | 2017-05-03 | 2017-06-20 | 黑龙江众生生物工程有限公司 | A kind of lactobacillus-fermented accelerator and its application for Juice fermentation |
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WO2013136768A1 (en) * | 2012-03-13 | 2013-09-19 | 株式会社明治 | Yogurt containing collagen and method for producing same |
CN104202988A (en) * | 2012-03-13 | 2014-12-10 | 株式会社明治 | Yogurt containing collagen and method for producing same |
JPWO2013136768A1 (en) * | 2012-03-13 | 2015-08-03 | 株式会社明治 | Fermented milk containing collagen and method for producing the same |
CN104202988B (en) * | 2012-03-13 | 2017-02-22 | 株式会社明治 | Yogurt containing collagen and method for producing same |
JP2013192470A (en) * | 2012-03-16 | 2013-09-30 | Snow Brand Milk Products Co Ltd | Food and drink containing lactobacillus with high survival rate and method for producing the same |
JP2015050993A (en) * | 2013-08-07 | 2015-03-19 | 国立大学法人 岡山大学 | Gastrointestinal tract and feces-derived lactobacilli culture milk or soybean milk-derived composition, culture or fermentation material and lactic fermentation product |
JP2018201405A (en) * | 2017-06-05 | 2018-12-27 | 雪印メグミルク株式会社 | Composition for survival improvement and/or growth promotion of lactic acid bacteria |
JP2022009842A (en) * | 2017-06-05 | 2022-01-14 | 雪印メグミルク株式会社 | Composition for survival improvement and/or growth promotion of lactic acid bacteria |
WO2021246392A1 (en) | 2020-06-02 | 2021-12-09 | アサヒグループホールディングス株式会社 | Lactic acid bacteria growth promoter |
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JP5964588B2 (en) | 2016-08-03 |
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HK1173182A1 (en) | 2013-05-10 |
IN2012DN02283A (en) | 2015-08-21 |
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