WO2011005801A1 - Elément de scellement luer pour colonnes d'extraction en phase solide - Google Patents

Elément de scellement luer pour colonnes d'extraction en phase solide Download PDF

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Publication number
WO2011005801A1
WO2011005801A1 PCT/US2010/041115 US2010041115W WO2011005801A1 WO 2011005801 A1 WO2011005801 A1 WO 2011005801A1 US 2010041115 W US2010041115 W US 2010041115W WO 2011005801 A1 WO2011005801 A1 WO 2011005801A1
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WO
WIPO (PCT)
Prior art keywords
luer
seal
spe
luer seal
columns
Prior art date
Application number
PCT/US2010/041115
Other languages
English (en)
Inventor
Philip A. Dimson
Bruce Redmond
Original Assignee
Speware Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Speware Corporation filed Critical Speware Corporation
Publication of WO2011005801A1 publication Critical patent/WO2011005801A1/fr
Priority to US13/346,044 priority Critical patent/US20120175368A1/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids

Definitions

  • the disclosed invention relates to devices and methods for Solid Phase Extraction (SPE).
  • SPE Solid Phase Extraction
  • Solid Phase Extraction is a sample preparation process using chromatographic micro columns for the extraction of nonvolatile or semi- volatile analytes from a liquid sample.
  • SPE is often used for the preparation of liquid samples prior to performing quantitative chemical analysis, for example, via high performance liquid chromatography (HPLC), or gas chromatography (GC).
  • HPLC high performance liquid chromatography
  • GC gas chromatography
  • SPE can also be used to separate solids that have been previously extracted into solvents.
  • target analytes can be isolated from a complex sample containing compounds that may interfere with the performance of a
  • a typical SPE tube is a cylindrical micro column or housing often formed from polypropylene or glass, and having a top inlet and a bottom outlet.
  • the SPE micro column is packed with a particulate medium referred to as a "sorbent,” a “resin,” a “solid phase,” or an “extraction phase,” terms which are used herein synonymously and interchangeably.
  • the bed of sorbent is packed between two porous frits, made, for example, of
  • a typical pore size of the frits is 20 micrometers (20 ⁇ m).
  • An SPE column with packed sorbent is pre-conditioned, typically with water and methanol using gravity, vacuum or positive pressure.
  • a liquid sample is first prepared in a container such as a test tube. To the test tube is added sample, buffer and an internal standard. The sample mixture is then transferred into the pre-conditioned SPE column. The sample fluid is typically poured in through the top inlet of the SPE column; the sample flows through the top frit, through the pre-conditioned sorbent, through the bottom frit, which is close to the bottom outlet, out the bottom outlet, and into a collection container. The fluid flow may be achieved by gravitational force, or by reduced pressure or vacuum applied at the lower end of the SPE column.
  • An SPE device may be in a single column or a multi-well format. Typical multi-well formats include 12, 24, 48, and 96 well or column array. Alternatively, the SPE micro column and a collection container may be placed in a centrifuge and centrifugal force used to cause the sample fluid go flow through the sorbent.
  • sorbent is based on which sample components are to be retained on the sorbent.
  • SPE at least one substance in a mixture of substances is initially adsorbed on the sorbent.
  • a sample is loaded onto a pre-conditioned sorbent.
  • a sequence of elution fluids are passed through the sorbent in the column to separate interferences from the target anaytes in the sample.
  • the removal of interferences such as unwanted molecules from the target analyte may comprise adhering such interfering compounds to the sorbent and allowing target analytes to remain in solution and elute through the column.
  • the target analytes adhere to the sorbent, and the interfering compounds are washed or eluted through the column. Following removal of the interfering compounds, a different solvent is used to remove the target analytes from the sorbent and to elute the target analytes through the column.
  • Samples must be placed in a test tube first before they are transferred into a conditioned SPE column.
  • An automated liquid handler can then be used to transfer a sample from a test tube into a SPE column.
  • the first samples that are transferred into the SPE columns start flowing immediately.
  • the automated liquid handler transfers the last sample into the last column, the first sample has completely run through the first column.
  • a device and method that can decrease sample preparation time and also eliminate the above-described problems by providing uniform processing of multi-well and muti column SPE columns.
  • the inventors of the disclosed subject matter have now developed a luer seal device for use with both a multi-well and a multi column SPE apparatus.
  • the disclosed luer seal is designed to maintain conditioning of the columns for the SPE process, and to maintain the samples intact within the columns until the user is ready to elute the samples through the columns.
  • the disclosed device and method provide for the flow of samples through each well or column of a multi-column SPE array of columns to begin substantially simultaneously.
  • a luer seal for sealing a plurality of solid phase extraction wells or columns in an array
  • the luer seal comprising: a flexible, substantially flat mat having a top surface and a bottom surface, the top surface having portions defining a plurality of female luer slip connectors spaced for receiving an open tip of at least one of the plurality of solid phase extraction wells or columns, and sealingly fastening thereto for preventing fluid flow through the open tip.
  • FIG. 1 is a schematic trans-sectional front elevational view of a 96 well SPE column device with attached SPE luer seal according to an embodiment of the invention.
  • FIG. 2 is a perspective view of a 96 well SPE column device in a 96 well SPE column holder showing a V-tab according to an embodiment of the invention.
  • FIG. 3 is a perspective view of a 96 well SPE column device in a 96 well SPE column holder showing SPE column luer tips, and SPE luer seal mat according to an embodiment of the invention.
  • FIG. 4A is a top plan view of a SPE luer seal mat according to an embodiment of the invention.
  • FIG. 4B is a front elevational view of a SPE luer seal mat according to an embodiment of the invention.
  • FIG. 4C is a side elevational view of a SPE luer seal mat according to an embodiment of the invention.
  • FIG. 5A is a front elevational view of a sealed SPE column holder with columns and SPE luer seal pressed onto the bottom of the SPE column luer tips according to an embodiment of the invention.
  • FIG. 5B is a front elevational view of a luer seal extractor according to an embodiment of the invention, the luer seal extractor shown with a sealed SPE column plate and ready to remove the SPE luer seal.
  • FIG. 5C is a side elevational view of a luer seal extractor according to an embodiment of the invention, the luer seal extractor shown with a sealed SPE column plate and ready to remove the SPE luer seal.
  • FIG. 5D is a cut-away top plan view of a luer seal extractor according to an embodiment of the invention, showing the extractor slide and direction of movement thereof needed to remove the luer seal from the SPE column plate.
  • the disclosed invention relates and applies to any SPE device that is used to direct liquid flow through the output of the column. As such, this tip is typically referred to as a "luer tip,” but the invention is not limited to a standard luer design.
  • Various embodiments of the disclosed luer seal can be used, for example, with small tips typical of a pre-molded set of 96 wells.
  • a male luer connector in general, has a tapered outer surface; and a female luer connector has a complementary tapered inner surface.
  • the tapering allows for connectors that are not precisely dimensioned to sealingly mate for either fluid transfer or to provide a seal to prevent fluid flow through a channel.
  • Luer connection systems have been previously used, for example, in medical devices for attaching two pieces of tubing to one another.
  • a luer connector may be used to attach tubing to a syringe, a catheter, or an IV tube.
  • luer connector is generally known by those of skill in the medical arts and chemical engineering arts, for example, as a connector that includes male and female rounded interlocking tubes, generally slightly tapered, so that the tubes fit together more securely.
  • a luer connection can be a simple pressure fit, referred to as a "luer slip” connector.
  • a "luer lock” connector provides a more secure fit, requiring a push and twist together motion for the connection.
  • a luer lock may have an outer, threaded rim that requires a twisting motion in order to seal, for a twisted or "screwed together" fit.
  • Our invention relates generally to a device that allows a rack of 48 SPE columns or 96 SPE columns that have been pre-conditioned, to be covered by a mechanical seal at the tapered or male luer end of each SPE column.
  • the 96 well format consists of 96 SPE columns in an 8 x 12 array.
  • a molded silicone rubber mat includes a plurality of spaced female luer slip connectors integral with the top surface of the mat, and spaced such that the female luer slip connectors can be aligned with the male luer column tips and press-fit onto the bottom of the array of columns, such that all 96 luer tips are sealed at the same instant.
  • This seal creates a compressible air gap between the SPE sorbent bed and the tip of the luer end of the column. Placed on the output end of the column, the luer seal mat prevents any solvent from flowing through the column, and allows the sorbent within the column to remain conditioned.
  • the entire column can act as a container for actually preparing the sample prior to the SPE separation.
  • This feature eliminates or minimizes the rate-limiting aspect of the sample preparation step in high throughput processing. It does so by enabling the user to use automated liquid-handling equipment to add the sample, the buffer and the internal standard directly into the SPE cartridge, without any of the sample passing through the sorbent bed and dripping from the bottom of the column.
  • the SPE luer seal mat can then be peeled off all of the luer tips, substantially
  • the luer seal extractor is a device that supports or cradles the sealed SPE plate or rack, and in one embodiment has a keyed slide with a built-in hook. As the user pulls the slide out, the hook catches a "V" tab that hangs down from the SPE luer seal. This single-hand motion allows the user to effortlessly remove the SPE luer seal completely without disturbing the column samples.
  • the quick removal of the SPE luer seal mat provides the user sufficient time to put the SPE rack or plate over a reservoir or a waste bin before the sample liquid begins to drip or flow through the column. Because the disclosed device provides a system and method for the quick removal of all the luer seals substantially simultaneously, the liquid will begin to flow in all of the columns substantially simultaneously, thereby ensuring more uniform processing of the columns than is possible with prior art devices..
  • the mechanical seal comprises a flexible, substantially flat mat, referred to herein as the "SPE luer seal mat,” having a top surface and a bottom surface, and a plurality of spaced female luer slip connectors integral with the top surface thereof.
  • the SPE luer seal mat is constructed of an elastomeric material, for example, silicone.
  • the SPE luer seal mat is a molded silicone rubber mat.
  • elastomeric material as these terms are used and understood by those of skill in the relevant arts is any one of a various polymers having the elastic properties of natural rubber.
  • the disclosed SPE luer seal may be
  • resilient refers to that property of a material that enables it to resume its original shape or position after being stretched, compressed, or bent.
  • Elastomeric and resilient as the terms are used herein, have similar meanings.
  • Non-limiting examples of other materials that may be suitable for use in constructing the SPE luer seal mat according to an embodiment of the invention include a polyester, a polypropylene, a perfluoroelastomer, a fluoroelastomer, a neoprene, a polyurethane, fluorosilicone, and
  • elastomeric materials for the SPE luer seal matt include VITON® (Du Pont de NeMours & Co., Delaware; a fluoroelastomer); HYTREL® (Du Pont de NeMours & Co., Delaware; a polyester elastomer); and AFLAS® (Asahi Glass Co., Limited, Tokyo, Japan; a heat and chemically resistant fluoro rubber.)
  • the elastomeric material may be coated with a thin jacket of a chemically resistant material such as polytetrafluoroethylene or fluorinated ethylene propylene (FEP).
  • a chemically resistant polymer jacket over an elastomer provides an outer layer of protection against corrosion combined with the memory of an elastomer when put under a compressive force.
  • FEP is a highly inert plastic, having low permeability to liquids, gases, moisture and organic vapors; non-flammability; high resistance to solvents; excellent anti-stick and low-frictional properties; and resistance to impact and tearing.
  • any FEP encapsulated or coated elastomeric material is suitable for use as the disclosed SPE luer seal mat.
  • the SPE luer seal mat has a maximum thickness of from about 0.002 inch (about 0.0051 centimeter) to about 0.250 inch (about 0.635 cm). In another embodiment of the invention, the flexible, substantially flat luer seal mat has a maximum thickness of about 0.080 inch (about 0.203 centimeter).
  • a luer seal for sealing a plurality of solid phase extraction columns in an array comprises a flexible
  • substantially flat mat having a top surface and a bottom surface, the top surface having portions defining a plurality of female luer slip connectors spaced for receiving an open tip of at least one of the plurality of solid phase extraction columns, and sealingly fastening thereto for preventing fluid flow through the open tip.
  • each of the female luer slip connectors of the disclosed luer seal has a tubular portion comprising: a proximal end having an annular opening at the top surface of the mat, the annular opening sized to accept the open tip of the solid phase extraction column and sealingly fasten thereto; and a closed distal end, and wherein the tubular portion is substantially perpendicular to the top surface of the mat.
  • the disclosed female luer slip connectors each comprise a
  • substantially cylindrical channel having an inner annular surface and a diameter sized to matingly engage with the outer surface of the SPE column tip, which column tip serves as the male luer slip connector.
  • the opening of the tubular body of each female luer slip connector has an approximate diameter of about 0.150 inch (0.381 cm) to fit the outside diameter of about 0.381 cm that is typical for a standard male luer tip of a micro column.
  • the channel of the female luer slip connector may be slightly tapered.
  • the female luer slip connectors on the SPE luer seal matt are spaced to match the position of the SPE column tips in the SPE multiple column array, which may be, for example a 96-well or a 48-well set of columns held in a 96-well or a 48-well column holder.
  • FIG. 1 is a schematic trans-sectional front elevational view 20 of a 96 well SPE column device with attached SPE luer seal 24 according to an embodiment of the invention.
  • the SPE multi-well column array comprises 96 SPE columns 10 in an eight column by 12 column (8 x 12) array.
  • FIG. 1 shows that each column 10 has a first filter fabric 16 and a second filter fabric 14 inserted within, and a sorbent bed 18 packed between the first and second filter fabrics 16, 14.
  • each column 10 has, below the first filter fabric 16, a slightly tapered, SPE column luer tip 12 having a slightly tapered internal bore.
  • FIG. 1 shows the channels comprising the spaced SPE female luer slip connectors 26 on top surface of SPE luer seal mat 24, the SPE female luer slip connectors 26 engaging the SPE column tips 12 to seal the columns 10.
  • the channels are the tubular portion of the female luer slip connector 26, and have a slightly tapered shape, wherein the proximal end has a larger diameter than the diameter of the distal end, such that the female luer slip connector 26 can form a press-fit with the open tip 12 of the solid phase extraction column 10, and sealingly fasten thereto.
  • the SPE column tip 12 functions as a male luer connector body having an external male luer tapered shape.
  • the SPE female luer slip connector 26 has an internal female Luer tapered shape substantially conforming to the external shape of the SPE column tip 12.
  • the SPE female luer slip connector 26 is capable of sealing the open SPE column tip 12 against fluid flow therethrough because the elastomeric material is biased against the open tip.
  • FIG. 1 depicts the SPE luer seal mat 24 press-fit onto the bottom of the array, having sealed all 96 SPE column luer tips 12 at substantially the same time.
  • Each column 10 as shown has an air gap within the SPE column luer tip 12.
  • This SPE luer seal mat 24 is applied to the bottom of the array after the SPE columns 10 have been pretreated, and remains in place until the columns 10 are ready for the elution step. Also shown are the channels 28 that are spaces between the tubular bodies of female luer slip connectors 26 on the bottom surface of luer seal mat 24. The bottom surface of the SPE luer seal mat 24 comprises the closed distal end of the tubular portion of at least one of the plurality of spaced female luer slip connectors 26.
  • the luer seal includes an uncoupling means 30 attached to the flexible, substantially flat mat 24 for use in removing the luer seal from the array of solid phase extraction columns 10.
  • the uncoupling means includes a V tab 30 attached to luer seal mat 24. The uncoupling means can be used to pull the luer seal mat 24 off of all the SPE column luer tips 12 at substantially the same time when the columns 10 are ready for the elution step in processing.
  • Non-limiting examples of the uncoupling means 30 include may be chosen from a tab, a V-tab, a loop, a handle, a hook, and combinations thereof. Any means of connecting the luer seal mat 24 to a mechanical device for pulling the luer seal mat 24 off of the SPE column luer tips 12 is envisioned as being within the scope of the disclosed invention.
  • the mechanical device may also be activated by a human hand.
  • each column 10 as shown has an air gap within the SPE column luer tip 12.
  • a partial vacuum is formed within the SPE column luer tip 12, the partial vacuum drawing any fluids within the column 10 through the SPE column luer tip 12.
  • the configuration of the disclosed SPE column luer seal mat 24 comprising an array of SPE female luer slip connectors 26 spaced to correspond to the positions of the array of the SPE columns 10 and an uncoupling means 30, enables the SPE operator to disengage each of the SPE female luer slip connectors 26 from the corresponding SPE column luer tips 12 substantially simultaneously, thereby allowing the fluid flow through each column to begin at approximately the same time, and all the columns 10 in the array to be processed substantially uniformly.
  • the means for moving the uncoupling means of the luer seal in a direction such that the luer seal is removed from the array of solid phase extraction columns may include a mechanical device.
  • a mechanical device includes a rail or other elongated support mounted on the inside bottom surface of a hollow housing; a slidable element, slidably mounted on the rail, the slidable element comprising a portion configured to engage the uncoupling means of the luer seal when the slidable element is in a first position, and to pull the luer seal away from the tips of the plurality of solid phase extraction columns in an array when the uncoupling means is moved in a direction parallel to the bottom surface of the mat to a second position.
  • FIG. 2 is a perspective view of a 96 well SPE column device 40 in a 96 well SPE column holder 36 showing the uncoupling means as a V-tab 30 according to an embodiment of the invention.
  • the array of columns 10 is supported by a 96 well column holder 36.
  • the column top opening 34 for receiving a fluid sample is shown.
  • FIG. 3 is different perspective view of a 96 well SPE column device 50 in a 96 well SPE column holder 36 showing SPE column luer tips 12 in the open bottom of the holder 36, and SPE Luer seal mat 38 with integral, spaced SPE female luer slip connectors 26 according to an embodiment of the invention. SPE Luer seal mat 38 is shown partially disengaged from the column tips 12.
  • FIG. 4A is a top plan view 52 of a SPE Luer seal mat 38 showing a V-tab attachment 42 according to an embodiment of the invention.
  • FIG. 4B is a front elevational view 54 of a SPE Luer seal mat 38 with V-tab attachment 42 according to an embodiment of the invention.
  • the spaces or channels 28 between the tubular bodies of the SPE female luer slip connectors 26 allow for expansion of the elastomeric material of the tubular body of the SPE female luer slip connector 24 such that it can form a tight seal with the column tip 12.
  • FIG. 4C is a side elevational view 56 of a SPE Luer seal mat according to an embodiment of the invention.
  • FIG. 5A is a front elevational view 58 of a 96 well SPE column holder 36 with sealed columns 10 and SPE Luer seal 24 pressed onto the bottom of the SPE column Luer tips (not shown) according to an embodiment of the invention.
  • FIGS. 5B through 5D depict another embodiment of the invention which includes a luer seal extractor for removing a luer seal from the tips of a plurality of solid phase extraction columns 10 in an array.
  • FIG. 5B is a front elevational view 60 of a Luer seal extractor according to an embodiment of the invention, the Luer seal extractor shown with a sealed SPE column array 10 supported by a column holder 36, and ready for the SPE Luer seal (not shown) to be removed.
  • the luer seal extractor 60 in the embodiment shown includes a hollow housing 46 having an inside top surface and an inside bottom surface, the housing 46
  • the luer seal extractor 60 includes a means for moving the uncoupling means 30, which in the embodiment shown is a V-tab 30, of the luer seal in a direction such that the luer seal is removed from the array of solid phase extraction columns 10.
  • the luer seal extractor 60 shown in FIG 5B includes a slide (not shown in this frontal view) with a slide handle 44, and a connector means, which in the embodiment shown is a hook 48 which is connected to or engaged with the V-tab 30 at the opposite or back end of the extractor 60.
  • the hook 48 is fixedly attached to the back end of the slide at the hook attachment point 45.
  • FIG. 5C is a side elevational view 62 of a luer seal extractor according to an embodiment of the invention, the luer seal extractor 62 shown with a sealed SPE column plate 10 and ready to remove the SPE luer seal (not visible).
  • the luer seal extractor 62 is shown with a partial cutaway of the luer seal extractor housing 46 to show the V-tab 30 of the SPE luer seal mat 24.
  • the extractor slide 43 is shown with its hook 48 mounted on the back portion of the extractor slide 43.
  • Slide handle 44 facilitates the movement of slide 43, and can be grasped by the operator's hand or moved by attaching to a motive device.
  • FIG. 5D is a cut-away top plan view 64 of a luer seal extractor according to an embodiment of the invention, showing the extractor slide 43 and direction of movement 70 thereof needed to remove the luer seal from the SPE column plate.
  • the luer seal extractor housing 46 is shown.
  • the column holder is not shown.
  • the hook 48 attached to the back end of the slide 43 is shown.
  • the luer seal extractor slide 43 is made of an aluminum shell extrusion, and a polypropylene T-Handle 44 is fastened to the front end of the slide 43 and a stainless steel hook 48 is fastened to the top- rear end of the slide 43.
  • the slide 43 in one embodiment is a shell extrusion shaped to ride on a polyethylene rail (not shown) that is attached to or molded with the bottom of the luer seal extractor housing 46.
  • the rail guides the slide 43 and positions it in the correct starting position.
  • the hook 48 catches the "V" Tab 30 that hangs down from the SPE luer seal mat 24, and removes the luer seal mat 24 from the column tips 12 substantially simultaneously.
  • phase layer of material is placed in the column before the column is packed with the sorbent. This layer of material will not allow liquid to pass thru unless the column is under pressure.
  • phase layer suitable for use in an embodiment of the invention include a membrane film or layer of pourous TEFLON® or polypropylene; and a membrane layer that functions as a duck bill type closure on the column that is molded from silicone rubber or similar polymers.

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  • Analytical Chemistry (AREA)
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  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention porte sur un élément de scellement pour sceller une pluralité de puits ou colonnes d'extraction en phase solide (SPE) dans un réseau, l'élément de scellement comprenant un support sensiblement plat, flexible ayant une surface supérieure et une surface inférieure. La surface supérieure a de multiples raccords à glissement femelles espacés pour recevoir les extrémités ouvertes des puits ou colonnes d'extraction en phase solide. Les raccords à glissement femelles se fixent de manière étanche sur les pointes ouvertes pour empêcher l'écoulement de fluide à travers les pointes ouvertes. L'invention porte également sur un extracteur d'élément de scellement pour retirer l'élément de scellement des pointes ouvertes sensiblement simultanément. Les pointes sont typiquement des raccords luer mâles ou des embouts de pipette.
PCT/US2010/041115 2009-07-08 2010-07-06 Elément de scellement luer pour colonnes d'extraction en phase solide WO2011005801A1 (fr)

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US13/346,044 US20120175368A1 (en) 2010-07-06 2012-01-09 Luer seal for solid phase extraction columns

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US22370809P 2009-07-08 2009-07-08
US61/223,708 2009-07-08

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
WO2022048715A1 (fr) * 2020-09-07 2022-03-10 Elbatron Gmbh Colonne luer

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US6479020B1 (en) * 1996-11-18 2002-11-12 Robbins Scientific Corporation Multi-well apparatus with independently detachable upper and lower covers
US20030094429A1 (en) * 1997-03-12 2003-05-22 Daikyo Seiko, Ltd. Sealing rubber closure for syringe/container
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US6479020B1 (en) * 1996-11-18 2002-11-12 Robbins Scientific Corporation Multi-well apparatus with independently detachable upper and lower covers
US20030094429A1 (en) * 1997-03-12 2003-05-22 Daikyo Seiko, Ltd. Sealing rubber closure for syringe/container
US6106783A (en) * 1998-06-30 2000-08-22 Microliter Analytical Supplies, Inc. Microplate assembly and closure
US6159368A (en) * 1998-10-29 2000-12-12 The Perkin-Elmer Corporation Multi-well microfiltration apparatus
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Publication number Priority date Publication date Assignee Title
WO2022048715A1 (fr) * 2020-09-07 2022-03-10 Elbatron Gmbh Colonne luer

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