WO2010140162A3 - A process for preparing stem cell based formulations - Google Patents

A process for preparing stem cell based formulations Download PDF

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Publication number
WO2010140162A3
WO2010140162A3 PCT/IN2010/000311 IN2010000311W WO2010140162A3 WO 2010140162 A3 WO2010140162 A3 WO 2010140162A3 IN 2010000311 W IN2010000311 W IN 2010000311W WO 2010140162 A3 WO2010140162 A3 WO 2010140162A3
Authority
WO
WIPO (PCT)
Prior art keywords
supernatant
stem cells
formulation
bone marrow
acellular
Prior art date
Application number
PCT/IN2010/000311
Other languages
French (fr)
Other versions
WO2010140162A2 (en
Inventor
Dravida Subhadra
Pisupati Sundai
Ram Kashyap Akshantala Venkata
Original Assignee
Dravida Subhadra
Pisupati Sundai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dravida Subhadra, Pisupati Sundai filed Critical Dravida Subhadra
Publication of WO2010140162A2 publication Critical patent/WO2010140162A2/en
Publication of WO2010140162A3 publication Critical patent/WO2010140162A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system

Abstract

The present invention discloses a process for preparing a formulation comprising stem cells the process comprises the steps of: subjecting a bone marrow aspirate to centrifugation to separate supernatant and cellular fraction comprising stem cells; isolating the stem cells; subjecting the supernatant to a plurality of centrifugation steps wherein following each centrifugation step the supernatant is freezed and thawed rapidly to remove cellular matter and obtain an acellular supernatant; reconstituting the stem cells with acellular supernatant. The formulation thus prepared comprises stem cells suspended in an acellular bone marrow supernatant which is devoid of any other type of contaminating cells such as T cells or cellular component, however it would have the extrinsic factors present in the bone marrow. The formulation is in a ready to use form for clinical application for use in stem cell based therapies.
PCT/IN2010/000311 2009-05-17 2010-05-17 A process for preparing stem cell based formulations WO2010140162A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN890/CHE/2009 2009-05-17
IN890CH2009 2009-05-17

Publications (2)

Publication Number Publication Date
WO2010140162A2 WO2010140162A2 (en) 2010-12-09
WO2010140162A3 true WO2010140162A3 (en) 2011-01-27

Family

ID=43063931

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2010/000311 WO2010140162A2 (en) 2009-05-17 2010-05-17 A process for preparing stem cell based formulations

Country Status (1)

Country Link
WO (1) WO2010140162A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102580152B (en) * 2012-03-09 2013-11-06 潘银根 Method for preparing acellular bone
CN111849880B (en) * 2020-06-23 2022-04-29 和携科技有限公司 Recovery method of human adipose mesenchymal stem cells after ultralow-temperature cryopreservation
CN115125192B (en) * 2022-09-02 2023-01-20 广州国家实验室 Bone marrow supernatant and application thereof in cell culture

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002171965A (en) * 2000-12-04 2002-06-18 Kansai Tlo Kk Method of separating and concentrating bone marrow monocyte cells and revascularizing agent
EP1872789A1 (en) * 2002-09-07 2008-01-02 Medcell Bioscience Ltd. Pharmaceuticals kits comprising mesenchymals stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002171965A (en) * 2000-12-04 2002-06-18 Kansai Tlo Kk Method of separating and concentrating bone marrow monocyte cells and revascularizing agent
EP1872789A1 (en) * 2002-09-07 2008-01-02 Medcell Bioscience Ltd. Pharmaceuticals kits comprising mesenchymals stem cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ASSMUS B ET AL: "Red Blood Cell Contamination of the Final Cell Product Impairs the Efficacy of Autologous Bone Marrow Mononuclear Cell Therapy", JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, ELSEVIER, NEW YORK, NY, US, vol. 55, no. 13, 30 March 2010 (2010-03-30), pages 1385 - 1394, XP026983698, ISSN: 0735-1097, [retrieved on 20100323] *
LAZARUS H M ET AL: "EX VIVO EXPANSION AND SUBSEQUENT INFUSION OF HUMAN BONE MARROW-DERIVED STROMAL PROGENITOR CELLS (MESENCHYMAL PROGENITOR CELLS): IMPLICATIONS FOR THERAPEUTIC USE", BONE MARROW TRANSPLANTATION, STOCKTON PRESS, GB, vol. 16, no. 4, 1 October 1995 (1995-10-01), pages 557 - 564, XP009036089 *
MIZUNO N ET AL: "Human autologous serum obtained using a completely closed bag system as a substitute for foetal calf serum in human mesenchymal stem cell cultures", CELL BIOLOGY INTERNATIONAL, ACADEMIC PRESS, GB, vol. 30, no. 6, 1 June 2006 (2006-06-01), pages 521 - 524, XP024917240, ISSN: 1065-6995, [retrieved on 20060601], DOI: DOI:10.1016/J.CELLBI.2006.01.010 *
SMITH J J ET AL: "Anabolic effects of acellular bone marrow, platelet rich plasma, and serum on equine suspensory ligament fibroblasts in vitro.", VETERINARY AND COMPARATIVE ORTHOPAEDICS AND TRAUMATOLOGY : V.C.O.T 2006 LNKD- PUBMED:16594543, vol. 19, no. 1, 2006, pages 43 - 47, XP002610098, ISSN: 0932-0814 *
SMITH ROGER K W: "Mesenchymal stem cell therapy for equine tendinopathy.", DISABILITY AND REHABILITATION 2008 LNKD- PUBMED:18608378, vol. 30, no. 20-22, 2008, pages 1752 - 1758, XP009141268, ISSN: 0963-8288 *

Also Published As

Publication number Publication date
WO2010140162A2 (en) 2010-12-09

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