WO2010137873A3 - 신종 인플루엔자 a형 바이러스 진단용 프라이머, 프로브, 이를 포함하는 키트 및 상기 키트를 이용한 진단 방법 - Google Patents

신종 인플루엔자 a형 바이러스 진단용 프라이머, 프로브, 이를 포함하는 키트 및 상기 키트를 이용한 진단 방법 Download PDF

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WO2010137873A3
WO2010137873A3 PCT/KR2010/003333 KR2010003333W WO2010137873A3 WO 2010137873 A3 WO2010137873 A3 WO 2010137873A3 KR 2010003333 W KR2010003333 W KR 2010003333W WO 2010137873 A3 WO2010137873 A3 WO 2010137873A3
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kit
primer
probe
diagnosis
new influenza
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PCT/KR2010/003333
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French (fr)
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WO2010137873A2 (ko
WO2010137873A9 (ko
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이윤경
조은진
황병오
변상진
김성열
박해준
박한오
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(주)바이오니아
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Publication of WO2010137873A2 publication Critical patent/WO2010137873A2/ko
Publication of WO2010137873A3 publication Critical patent/WO2010137873A3/ko
Publication of WO2010137873A9 publication Critical patent/WO2010137873A9/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)

Abstract

본 발명은 신종 인플루엔자 A형 H1N1 서브타입 바이러스(2009 H1N1 인플루엔자 바이러스)의 유전자에 특이적인 프라이머, 프로브 및 상기 프라이머와 프로브를 이용하여 신종 인플루엔자 A형 H1N1 서브타입 바이러스를 진단하는 방법에 관한 것으로, 보다 상세하게는 신종 인플루엔자 A형 H1N1 서브타입 바이러스의 유전자를 중합효소연쇄반응으로 동시에 진단하기 위한 프라이머, 인터날 컨트롤 및 그 프라이머를 포함하는 진단 키트 및 그 키트를 이용한 진단 방법에 관한 것이다. 본 발명의 프라이머 및 프로브를 이용하면, 기존의 인체 인플루엔자 A형 H1N1 바이러스와의 교차반응 없이 신종 인체 인플루엔자 A형 H1N1 서브타입 바이러스를 신속하고 정확하게 검출할 수 있으며, 보관 안전성이 향상되고, 사용을 위해 검사자의 숙련도가 요구되지 않는 건조된 키트를 제조할 수 있다.
PCT/KR2010/003333 2009-05-26 2010-05-26 신종 인플루엔자 a형 바이러스 진단용 프라이머, 프로브, 이를 포함하는 키트 및 상기 키트를 이용한 진단 방법 WO2010137873A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020147007988A KR101411412B1 (ko) 2009-05-26 2010-05-26 신종 인플루엔자 a형 바이러스 진단용 프라이머, 프로브, 이를 포함하는 키트 및 상기 키트를 이용한 진단 방법

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20090046211 2009-05-26
KR10-2009-0046211 2009-05-26

Publications (3)

Publication Number Publication Date
WO2010137873A2 WO2010137873A2 (ko) 2010-12-02
WO2010137873A3 true WO2010137873A3 (ko) 2011-04-14
WO2010137873A9 WO2010137873A9 (ko) 2011-06-03

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PCT/KR2010/003333 WO2010137873A2 (ko) 2009-05-26 2010-05-26 신종 인플루엔자 a형 바이러스 진단용 프라이머, 프로브, 이를 포함하는 키트 및 상기 키트를 이용한 진단 방법

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KR (2) KR101411412B1 (ko)
WO (1) WO2010137873A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102154511B (zh) * 2011-03-14 2013-01-02 秦智锋 一步法检测新型甲型h1n1 2009流感病毒的试剂制备方法和应用
KR101780033B1 (ko) * 2013-03-07 2017-09-19 대한민국(관리부서 질병관리본부장) 노로바이러스 검출용 프라이머, 프로브 및 이를 이용한 검출키트 및 방법
KR102174967B1 (ko) 2018-11-15 2020-11-05 고려대학교 산학협력단 인플루엔자 바이러스 검출용 프라이머 세트, 이를 포함하는 검출용 키트 및 이를 이용한 검출 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070184434A1 (en) * 2005-10-17 2007-08-09 Rangarajan Sampath Compositions for use in identification of influenza viruses
US20090124512A1 (en) * 2006-01-18 2009-05-14 Regents Of The University Of Colorado Dna array analysis as a diagnostic for current and emerging strains of influenza

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070184434A1 (en) * 2005-10-17 2007-08-09 Rangarajan Sampath Compositions for use in identification of influenza viruses
US20090124512A1 (en) * 2006-01-18 2009-05-14 Regents Of The University Of Colorado Dna array analysis as a diagnostic for current and emerging strains of influenza

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AIZHONG HU ET AL.: "Simultaneous Detection, Sub grouping, and Quantitation of Respiratory Syncytial Virus A and B by Real-Time PCR.", JOURNAL OF CLINICAL MICROBIOLOGY., vol. 41, no. 1, 2003, pages 149 - 154 *
B. SCHWEIGER ET AL.: "Application of a Fluorogenic PCR Assay for Typing and Subtyping of Influenza Viruses in Respiratory Samples.", JOURNAL OF CLINICAL MICROBIOLOGY., vol. 38, no. 4, 2000, pages 1552 - 1558 *
DATABASE GENBANK 6 May 2009 (2009-05-06), "Influenza A virus (A/California/04/2009(H1N1)) segment 4 hemagglutinin (HA) gene, complete cds", Database accession no. GQ117044 *
MICHAEL J. CARR ET AL.: "Rapid molecular detection of the H275Y oseltamivir resistance gene mutation in circulating influenza A (H1N1) viruses.", JOURNAL OF VIROLOGICAL METHODS., vol. 153, no. ISS.2, 2008, pages 257 - 262, XP025495498, DOI: doi:10.1016/j.jviromet.2008.07.011 *

Also Published As

Publication number Publication date
KR101411412B1 (ko) 2014-06-25
KR20120024944A (ko) 2012-03-14
KR20140043848A (ko) 2014-04-10
WO2010137873A2 (ko) 2010-12-02
WO2010137873A9 (ko) 2011-06-03

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