WO2010134095A3 - Yeast strain expressing modified human cytochrome p450 reductase and/or cytochrome p450 gene - Google Patents

Yeast strain expressing modified human cytochrome p450 reductase and/or cytochrome p450 gene Download PDF

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Publication number
WO2010134095A3
WO2010134095A3 PCT/IN2010/000321 IN2010000321W WO2010134095A3 WO 2010134095 A3 WO2010134095 A3 WO 2010134095A3 IN 2010000321 W IN2010000321 W IN 2010000321W WO 2010134095 A3 WO2010134095 A3 WO 2010134095A3
Authority
WO
WIPO (PCT)
Prior art keywords
cytochrome
yeast strain
reductase
human cytochrome
gene
Prior art date
Application number
PCT/IN2010/000321
Other languages
French (fr)
Other versions
WO2010134095A2 (en
Inventor
Rajeev Soni
Prabuddha Kumar Kundu
Kajal Arora
Purna Chandra Rao
Kunal Bhalla
Sunil Kumar Suman
Vipul Kumar
Original Assignee
Premas Biotech Pvt. Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Premas Biotech Pvt. Ltd filed Critical Premas Biotech Pvt. Ltd
Publication of WO2010134095A2 publication Critical patent/WO2010134095A2/en
Publication of WO2010134095A3 publication Critical patent/WO2010134095A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides modified polynucleotide sequences coding for human cytochrome P450 reductase, human cytochrome P450 and human cytochrome b5. Further a genetically modified protease deficient yeast strain comprising integrated modified human cytochrome P450 reductase gene is provided in the present invention. The present invention also provides a genetically modified protease deficient yeast strain comprising integrated modified human cytochrome P450 reductase gene and a plasmid comprising modified polynucleotide sequence coding for cytochrome P450s and/or modified polynucleotide sequence coding for cytochrome b5. The yeast strain(s) disclosed in the present invention have been shown to express catalytically active cytochrome P450s with higher specific activity.
PCT/IN2010/000321 2009-05-19 2010-05-19 Yeast strain expressing modified human cytochrome p450 reductase and/or cytochrome p450 gene WO2010134095A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1017DE2009 2009-05-19
IN1017/DEL/2009 2009-05-19

Publications (2)

Publication Number Publication Date
WO2010134095A2 WO2010134095A2 (en) 2010-11-25
WO2010134095A3 true WO2010134095A3 (en) 2011-03-17

Family

ID=42790691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2010/000321 WO2010134095A2 (en) 2009-05-19 2010-05-19 Yeast strain expressing modified human cytochrome p450 reductase and/or cytochrome p450 gene

Country Status (1)

Country Link
WO (1) WO2010134095A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201322740D0 (en) * 2013-12-20 2014-02-05 Monfort University De Methods
CA3169961A1 (en) * 2020-02-03 2021-08-12 Premas Biotech Private Limited Recombinant expression platform, constructs and methods for expression of difficult to express proteins (dte-ps)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635369A (en) * 1991-07-15 1997-06-03 Centre National De La Recherche Scientifique Yeast strains with stable integration of heterologous genes
US6579693B1 (en) * 1995-09-15 2003-06-17 Aventis Pharma S.A. Genetically engineered yeast strains
WO2007129050A2 (en) * 2006-05-05 2007-11-15 De Montfort University Method for production of cytochrome p450 with n-term. truncated p450 reductase
WO2008152088A1 (en) * 2007-06-12 2008-12-18 Pombiotech Gmbh Fission yeast expressing cytochrome p450 reductase

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635369A (en) * 1991-07-15 1997-06-03 Centre National De La Recherche Scientifique Yeast strains with stable integration of heterologous genes
US6579693B1 (en) * 1995-09-15 2003-06-17 Aventis Pharma S.A. Genetically engineered yeast strains
WO2007129050A2 (en) * 2006-05-05 2007-11-15 De Montfort University Method for production of cytochrome p450 with n-term. truncated p450 reductase
WO2008152088A1 (en) * 2007-06-12 2008-12-18 Pombiotech Gmbh Fission yeast expressing cytochrome p450 reductase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHENG J ET AL: "Establishment of a yeast system that stably expresses human cytochrome P450 reductase: Application for the study of drug metabolism of cytochrome P450s in vitro", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA LNKD- DOI:10.1016/J.PEP.2005.11.022, vol. 47, no. 2, 1 June 2006 (2006-06-01), pages 467 - 476, XP024908767, ISSN: 1046-5928, [retrieved on 20060601] *
URBAN P ET AL: "Maximizing the expression of mammalian cytochrome P-450 monooxygenase activities in yeast cells", BIOCHIMIE, MASSON, PARIS, FR LNKD- DOI:10.1016/0300-9084(90)90070-W, vol. 72, no. 6-7, 1 June 1990 (1990-06-01), pages 463 - 472, XP023480786, ISSN: 0300-9084, [retrieved on 19900601] *

Also Published As

Publication number Publication date
WO2010134095A2 (en) 2010-11-25

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