WO2010128802A2

WO2010128802A2 - Composition comprising snowberry extract

Info

Publication number: WO2010128802A2
Application number: PCT/KR2010/002866
Authority: WO
Inventors: 이찬우; 이수진; 김한성; 전상훈; 김진웅; 김한곤
Original assignee: (주)아모레퍼시픽
Priority date: 2009-05-07
Filing date: 2010-05-06
Publication date: 2010-11-11
Also published as: CN102413812A; KR20100121000A; CN102413812B; WO2010128802A3; KR101601908B1; HK1167824A1

Abstract

Disclosed is a composition comprising snowberry extract, which is effective in preventing or alleviating wrinkles, in attenuating or suppressing inflammation, in whitening, moisturisation and oxidation resistance and in preventing or alleviating ageing.

Description

Composition containing snowberry extract

The present invention relates to a composition containing a snowberry extract.

As humans age, they change due to various internal and external factors. As we age, the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which make up the skin, change or decrease, and oxidative stress is caused by free radicals and free radicals. In addition, due to the progress of aging or ultraviolet light, inflammatory factors increase, and the biosynthesis of matrix metalloproteinase (MMP), an enzyme that breaks down skin tissues by these inflammatory factors, increases in inducible nitric oxide synthase (iNOS). NO production is known to increase.

On the other hand, the skin color of a person is determined by various factors. Among them, factors such as melanocyte (melanocyte) activity, the distribution of blood vessels, the thickness of the skin and the presence or absence of pigments, such as carotenoids, bilirubin, etc. are important. The most important factor among them is a black pigment called melanin, which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. Melanin, which is produced in the skin by external stress stimuli, is a stable substance that does not disappear until it is released to the outside through skin keratinization even when the stress disappears. If this melanin occurs more than necessary, it causes hyperpigmentation such as blemishes, freckles, and spots, resulting in cosmetically bad results.

In addition, PPARγ, which is present in skin constituent cells and regulates energy homeostasis, plays an important role in maintaining and restoring homeostasis of skin barrier function, moisturizing ability, and inflammatory healing process. It is known to be involved in the regulation of skin conditions such as controlling the permeability of the skin barrier, inhibiting epidermal layer proliferation, inducing differentiation of the epidermal layer through various mechanisms. Due to these characteristics, PPARγ may act as a key regulator of various skin diseases such as psoriasis, wound healing, acne due to overgrowth of the epidermal layer as well as inflammation-related skin diseases.

The present invention is to provide a composition used for preventing or improving wrinkles, alleviating or inhibiting inflammation, whitening, moisturizing, preventing or improving wrinkles by containing a snowberry extract.

One aspect of the invention provides a composition containing a snowberry extract.

In one aspect of the invention, the composition has a use to prevent or improve skin wrinkles.

In one aspect of the invention, the composition has a use to alleviate or inhibit inflammation.

In one aspect of the invention, the composition has a skin whitening use.

In one aspect of the invention, the composition has a skin moisturizing use.

In one aspect of the invention, the composition has antioxidant use.

In one aspect of the invention, the composition has a use to prevent or improve aging.

The composition according to one aspect of the present invention may have an effect of preventing or improving wrinkles, alleviating or inhibiting inflammation, whitening, moisturizing, antioxidant, preventing or improving aging by containing a snowberry extract as an active ingredient.

Figure 1 is a graph of the antioxidant power measurement results using the DPPH of the seven kinds of snowberry extract and vitamin C.

As used herein, the term "extract" includes any material obtained by extracting a component therefrom from a natural product, regardless of the method of extraction or the kind of the component. For example, it is a broad concept that includes all the components which are dissolved in a solvent from a natural product using water or an organic solvent, and those obtained by extracting only a specific component such as an oil such as oil.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is a broad concept including not only tissues covering the body surface such as the face or body, but also the scalp and hair.

Snowberry is a plant of the genus Symphoricarpos and is a deciduous shrub to the Anthropophyte and includes about 15 species. All but one are native to North and Central America, and the other is of western China. Also called waxberry. Fruits with a diameter of 1-2 cm are soft, depending on the type, from white (e.g. common snowberry) to pink (little leaf snowberry), red (coral berry) and blackish purple (chinese coral) Different colors. Typical types of snowberries are as follows.

Common snowberry ( Symphoricarpos albus ),

Mexican snowberry ( Symphoricarpos mexicanus ),

Little leaf snowberry ( Symphoricarpos microphyllus),

Creeping snowberry ( Symphoricarpos mollis ),

Western snowberry or Wolfberry, Symphoricarpos occidentalis ,

Coralberry, Symphoricarpos orbiculatus ,

Mountain snowberry, Symphoricarpos oreophilus ,

Roundleaf snowberry ( Symphoricarpos rotundifolius),

Chinese coralberry ( Symphoricarpos sinensis ).

One aspect of the invention discloses a composition containing a snowberry extract as an active ingredient.

In one aspect of the invention, the snowberry includes all kinds of snowberries. In another aspect of the invention, the snowberry is a common snowberry (Symphoricarpos albus), Mexican SnowberrySymphoricarpos mexicanus), Little Leaf SnowberrySymphoricarpos microphyllus), Creeping snowberry (Symphoricarpos mollis), Western snowberry (Symphoricarpos occidentalis), Coral berry (Symphoricarpos orbiculatus), Mountain snowberry (Symphoricarpos oreophilus), Roundleaf Snowberry (Symphoricarpos rotundifolius) And Chinese Coral Berry (Symphoricarpos sinensisIt may include one or more selected from the group consisting of), but is not limited thereto.

In one aspect of the invention, the snowberry extract can be obtained by extracting the snowberry in a conventional manner. In another aspect of the present invention, the extract may be obtained by heating and extracting the snowberry in an organic solvent including water or alcohol, and then filtration and concentration under reduced pressure. In another aspect of the present invention, the organic solvent is not particularly limited, and may be a C ₁ ~ C ₅ lower alcohol. The lower alcohol of C ₁ ~ C ₅ may be, for example, any one or two or more mixed solvents selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol. .

In one aspect of the invention, the snowberry extract may be contained in 0.1 to 50% by weight based on the total weight of the total composition. In another aspect of the present invention, the snowberry extract may be contained in 0.1 to 30% by weight based on the total weight of the total composition. In another aspect of the invention, the snowberry extract may be contained in 0.1 to 10% by weight based on the total weight of the total composition. When contained in the above range, it is not only suitable for showing the intended effect of the present invention, but also satisfies both the stability and safety of the composition, it may be appropriate to use in the above range in terms of cost-effectiveness.

The compositions disclosed herein contain snowberry extracts that are potent in promoting procollagen biosynthesis and inhibiting the production of MMP-1, thereby preventing or improving skin wrinkles to inhibit skin aging.

In addition, since the snowberry extract inhibits the production of NO, the composition containing the snowberry extract can alleviate or suppress inflammation and prevent or improve aging.

Snowberry extract can whiten the skin by inhibiting the production of melanin. Therefore, the composition containing the snowberry extract can be used for skin whitening purposes.

PPARγ maintains epidermal homeostasis by regulating epidermal keratinocyte differentiation and lipid metabolism to induce normal epidermal layer formation. Therefore, when PPARγ is activated, it can be expected to moisturize the skin by enhancing skin barrier function. Since the snowberry extract activates such PPARγ, the composition containing the same has a moisturizing effect on the skin and may further prevent or improve skin aging.

The composition according to one aspect of the present invention by containing a snowberry extract having an antioxidant effect as an active ingredient, it is possible to prevent damage to cells caused by free radicals or ultraviolet rays, thereby preventing or improving aging.

One aspect of the present invention provides a cosmetic composition comprising a composition containing a snowberry extract. The cosmetic form accordingly contains a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules, ionic (liposomes) and non-obtained by dispersing an oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases. It may be provided in the form of an aerosol further containing ionic vesicle dispersants, foams or compressed propellants. These compositions can be prepared according to conventional methods in the art.

Cosmetic compositions include fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers , Metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or auxiliaries commonly used in the cosmetic or dermatological fields. have. Such adjuvants are included in amounts generally used in the cosmetic or dermatological arts.

The cosmetic composition is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, It can be formulated into cosmetics such as packs, powders, body lotions, body creams, body oils and body essences.

Another aspect of the invention provides a pharmaceutical composition comprising a composition containing a snowberry extract. Such pharmaceutical compositions may further comprise pharmaceutical adjuvants such as preservatives, stabilizers, hydrating or emulsifying accelerators, salts and / or buffers for the control of osmotic pressure and other therapeutically useful substances, and can be used in various oral forms according to conventional methods. It may be formulated in the form of a dosage form or parenteral dosage form.

The oral dosage forms include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, powders, powders, fine granules, granules, pellets, and the like, and these formulations include a surfactant in addition to the active ingredient. , Diluents (eg lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), glidants (eg silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycols) . Tablets may also include binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally starch, agar, alginic acid or its sodium salt It may include pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, and sweeteners. The tablets can be prepared by conventional mixing, granulating or coating methods.

In addition, the parenteral administration agent may be, for example, formulations such as injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, patches, and the like. no.

The pharmaceutical composition according to the present invention can be administered orally, parenterally, for example, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous.

In addition, the dosage of the active ingredient will vary depending on the age, sex, weight of the subject to be treated, the pathology and severity of the particular disease to be treated, the route of administration or the judgment of the prescriber. Dosage determination based on these factors is within the level of skill in the art. Typical dosages are 0.001 mg / kg / day to 2000 mg / kg / day, more specifically 0.5 mg / kg / day to 2.5 mg / kg / day.

Another aspect of the invention provides a health food composition comprising a composition containing a snowberry extract. The health food composition is not particularly limited in the formulation, for example, it may be formulated as a tablet, granules, drinks, caramel, diet bar and the like. In addition to the active ingredient, the health food composition of each formulation may be suitably selected by a person skilled in the art according to the formulation or purpose of use, in addition to the active ingredient, and may be synergistic when applied simultaneously with other raw materials. .

Determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage of the drug depends on various factors such as the state of health, time of onset, age, complications, etc. As the composition 1 to 500mg / kg, preferably 30 to 200 mg / kg may be administered by dividing once or twice a day, the dosage does not limit the scope of the invention in any way. .

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to Examples and Test Examples. However, these Examples and Test Examples are provided only for the purpose of illustration in order to help the understanding of the present invention is not limited to the scope of the present invention.

EXAMPLE

Common snow berry, little leaf snow berry, creeping snow berry, Western snow berry, Coral berry, mountain snow berry, and 7 kinds of snow berry, such as Chinese snow berry was extracted in a conventional manner to prepare the extract as an example.

Test Example 1 Procollagen Biosynthesis

Human skin was harvested and seeded with fibroblasts directly cultured in primary cells at 10 ⁶ per hole in 24 holes and cultured until 90% growth. After incubation in serum-free medium for 24 hours, vitamin C and each snowberry extract was dissolved in serum-free medium, treated to 100 ppm concentration, and incubated in a CO ₂ incubator for 24 hours. The supernatant was removed and procollagen was increased or decreased using the procollagen type (I) Elisa kit (Procollagen type 1). The test was conducted at concentrations considering cytotoxicity, and the results are shown in Table 1. Synthetic capacity is prepared by the non-treated group 100.

Table 1

Test substance	Synthetic Performance (%)
Untreated group	100
Vitamin c	128.5
Common Snowberry (S. albus)	118.4
Little Leaf Snowberry (S. microphyllus)	113.4
Creeping Snowberry (S. mollis)	117.1
Western Snowberry (S. occidentalis)	105.2
Coral berry (S. orbiculatus)	97.4
Mountain snowberry (S. oreophilus)	116.7
Chinese Coral Berry (S. sinensis)	104.5

As can be seen in Table 1, the procollagen synthesis ability of most snowberry extracts was superior to the untreated group. Therefore, the composition containing the snowberry extract can alleviate the collagen reduction caused by aging of the human skin by promoting collagen production.

Test Example 2 Collagenase (MMP-1) Inhibitory Activity

Fibroblasts collected directly from human skin from 96-well microtiter plates containing DMEM (Dulbecco's Modified Eagle's Media) containing 2.5% fetal bovine serum were cultured 5,000 cells / Into a well (well), it was incubated until 70 ~ 80% growth. And retinoic acid and each snowberry extract was treated for 24 hours at a concentration of 10 ppm, and then the cell culture was collected. The cultured cell culture solution was measured by using a commercially available collagenase measuring instrument (Amersham Pharmacia Co., Catalog #: RPN 2610). First, the cell culture medium was placed in a 96-hole plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours. After 3 hours, the chromophore-conjugated secondary collagen antibody was placed in a 96-hol plate and reacted again for 15 minutes. After 15 minutes, the coloring stimulant was added to cause color development at room temperature for 15 minutes, and 1M sulfuric acid was added again to stop the reaction (color development), thereby making the reaction solution yellow. At this time, the degree of yellow appears differently depending on the progress of the reaction. The absorbance of the yellowish 96-hole plate was measured at 405 nm using an absorbance meter, and the degree of synthesis of collagenase was calculated by the following equation (1). At this time, the reaction absorbance of the cultured cell culture medium of the group that was not treated as a control was used as a control. The test was carried out at a concentration in consideration of cytotoxicity, and the results are shown in Table 2.

Equation 1

TABLE 2

Test substance	Collagenase expression suppression degree (%)
Untreated group	100
UV treatment	152.4
Retinoic acid	84.3
Common Snowberry (S. albus)	82.4
Little Leaf Snowberry (S. microphyllus)	78.1
Creeping Snowberry (S. mollis)	103.5
Western Snowberry (S. occidentalis)	85.6
Coral berry (S. orbiculatus)	93.9
Mountain snowberry (S. oreophilus)	81.6
Chinese snowberry (S. sinensis)	101.5

As the inhibition of collagenase expression increases, the percentage decreases. As shown in Table 2, the collagenase matrix metalloprotease (MMP-1) was inhibited in most snowberry extracts. That is, the composition containing snowberry extract may inhibit the biosynthesis of MMP-1, a skin tissue degrading enzyme caused by internal aging or external environmental factors, thereby inhibiting collagen reduction in skin and improving wrinkles.

Test Example 3 Anti-inflammatory Activity Measurement (Cell-Based Analysis)

Unprocessed 246.7 macrophage cell lines (obtained from Korea Cell Line Bank) were placed in DMEM medium with 10% FBS penicillin (100 IU / mL) and streptomycin (100 μg / mL), followed by a temperature 37 ^° C., 5% CO ₂ incubator. Cells were grown at and 300 μl were dispensed at a density of 5 × 10 ⁵ cells / ml on a 48-hole plate, and fractions were treated by concentration after 24 hours. After 1 hour, LPS was treated with 1 μg / ml, and the amount of NO released in the medium after 18 hours was measured using a Greis reagent. Was a group treated with dexamethasone for 10uM as a positive control of an anti-inflammatory activity of inhibiting the release of NO, and then the amount of NO as compared with the determined measured value of the standard NaNO ₂ the results are shown in Table 3.

TABLE 3

ingredient	Extract concentration (ppm)	% Inhibition
Positive control	-	100
LPS 1 µg / ml	-	0
1 μg / ml LPS + common snowberry (S. albus)	50	40.7
1 μg / ml LPS + little leaf snowberry (S. microphyllus)	50	41.6
1 μg / ml LPS + creeping snowberry (S. mollis)	50	32.1
1 μg / ml LPS + Western Snowberry (S. occidentalis)	50	29.6
1 μg / ml LPS + coralberry (S. orbiculatus)	50	36.1
1 μg / ml LPS + Mountain Snowberry (S. oreophilus)	50	33.3
1 µg / ml LPS + Chinese Coral Berry (S. sinensis)	50	41.6

As shown in Table 3, all the snowberry extract was found to have an anti-inflammatory effect because it inhibits NO production compared to the negative control.

Test Example 4 Evaluation of Melanin Inhibitory Activity Using Melan-a Cells

Melan-a cells (obtained from St. George's Hospital Medical School, UK) were plated in DMEM containing 10% FBS in 6-hole plates (melanin quantification) in 2 x 10 ⁴ cells / ball (final volume 3 ml), 96-hole plates (MTTassay) was added 2 × 10 ³ cells / ball (final volume 200 μl) and incubated for 24 hours in 37 ℃, 5% CO ₂ incubator (MCO-20AIC, Sanyo). After incubation, the medium was removed, and DMEM medium containing 10% FBS, 2 μM α-MSH (melanosite stimulating hormone), 2 mM theophylline was used as a fresh medium and each snowberry extract of Example was added to the medium for 3 days daily. Treated. On day 5, the cultured medium was removed from the 6-hole plate and treated with 0.25% Trypsin-EDTA (Sigma Chemical Co., Ltd.) solution to recover the cell pellet, transferred to a 1.5 ml test tube, and centrifuged at 10,000 rpm for 10 minutes to remove the supernatant. Removed. The obtained pellet was dried at 60 ° C, and 100 µl of 1N NaOH was added to dissolve the melanin in the cells. The solution was diluted with PBS and then absorbance was measured at 475 nm of ELISA reader to determine the rate of melanin production of each test substance. Cells without snowberry extract were used as negative controls, and kojic acid and melansolve were used as positive controls. The whitening effect was evaluated by measuring the melanin production inhibition of snowberry extract compared to the melanin content in the negative control. To calculate the melanin production inhibition rate according to the following equation 2 and the results are shown in Table 4.

Equation 2

Table 4

Test substance	density	Melanin production inhibition rate (%)
Untreated group	No addition	-
Kojic acid	100 ppm	49.7%
Melasolve
	100 ppm	72.8%
Common Snowberry (S. albus)	100 ppm	52.1%
Little Leaf Snowberry (S. microphyllus)	100 ppm	43.6%
Creeping Snowberry (S. mollis)	100 ppm	29.8%
Western Snowberry (S. occidentalis)	100 ppm	21.5%
Coral berry (S. orbiculatus)	100 ppm	34.5%
Mountain snowberry (S. oreophilus)	100 ppm	50.8%
Chinese Coral Berry (S. sinensis)	100 ppm	32.4%

As can be seen from Table 4, melanin production inhibitory effect was recognized in most snowberry extracts. Through this, the snowberry extract was confirmed to have an excellent whitening effect.

Test Example 5 Evaluation of PPARγ Efficacy

1) Cell Culture

Normal African green monkey kidney epithelial cells, CV-1 cells (available from ATCC) were obtained from DMEM (Dulbecco Modified) containing 10% Fetal Bovine Serum and 100 unit / ml penicillin-streptomycin. Eagle's Medium) and incubated at 37 ℃, 5% CO ₂ .

2) Plasmid transfection

The plasmid has a PPARγ plasmid expressing PPARγ and a PPARs Response Element (PPRE) activated by binding to PPARs under normal culture conditions, and then firefly luciferase acting as a reporter. PPRE-Luc plasmids with genes and three β-gal plasmids used to correct for transfection were used.

Dispense CV-1 cells into a 24-hole plate at a concentration of 5 x 10 ⁴ and incubate for 18-24 hours. Reaction of the plasmids with a 1: 3 (μg: μl) mixture of DMEM and PEI (Polyethylenimine) at room temperature for 15 minutes After the plasmids were introduced into the cells by spraying the respective balls.

3) PPAR-γ activity measurement

After plasmids were introduced into the cells and incubated for 24 hours to stabilize, the test materials were treated and incubated for another 24 hours. After incubation, the cells were washed twice with cold PBS, and the cells were destroyed by treating the cells with reporter lysis buffer (Promega) for 15 minutes. The cell eluate thus obtained was transferred to a micro tube and centrifuged at 12,000 rpm for 3 minutes at 4 ° C. to separate cell residues, thereby obtaining pure cell eluate.

PPAR-γ activity of each test group was compared by mixing Luciferase substrate (Promega) in the cell eluate in a 2: 1 ratio and measuring the amount of light emitted by the luminometer. In addition, each test group was treated with the same amount of 2X β-galactosidase enzyme assay buffer (Promega) in the cell eluate and reacted at 37 ° C. for 30 minutes, and then measured for absorbance at 420 nm. The beta-galactosidase activity of was compared. The PPAR-γ activity of each test group was divided by the activity of each beta-galactosidase to correct the difference according to the degree of plasmid introduction, and this correction value was evaluated using this sample t test of Minitab. .

Table 5

Test substance	density	Activity (%)
Untreated group	No addition	100%
TGZ	5 M	600%
Common Snowberry (S. albus)	100 ppm	263%
Little Leaf Snowberry (S. microphyllus)	100 ppm	139%
Creeping Snowberry (S. mollis)	100 ppm	213%
Western Snowberry (S. occidentalis)	100 ppm	183%
Coral berry (S. orbiculatus)	100 ppm	152%
Mountain snowberry (S. oreophilus)	100 ppm	235%
Chinese Coral Berry (S. sinensis)	100 ppm	143%

As can be seen in Table 5, it was confirmed that all of the snowberry extract PPARγ activity is excellent compared to the untreated group has excellent skin moisturizing effect.

Test Example 6 Evaluation of Antioxidant (DPPH) Efficacy

100g of the snowberry extract dry weight of the Example was prepared by dissolving to a concentration of 10% in DMSO. As a positive control, vitamin C was dissolved in water to make 1%. DPPH (1,1-diphenyl-2-picrylhydrazyl) was prepared to have a concentration of 100 μM in ethanol. After diluting the extract solution and the vitamin C solution by 1/2 step with ethanol to 5 concentrations, 10 μl of each solution and 190 μl of DPPH solution were mixed and incubated at 37 ° C. for 30 minutes. Thereafter, the OD value was measured at 540 nm. The measured values are shown in FIG. 1. In FIG. 1, the lower the Y-axis value is 100, the greater the antioxidant capacity.

Therefore, it was confirmed that the antioxidant efficacy of the snowberries are superior to the negative control.

Examples of the formulation of the composition according to the present invention are described below, but are also applicable to various other formulations, which are intended to explain in detail only and not intended to limit the present invention.

Formulation Example 1 Flexible Lotion

To a flexible lotion was prepared in a conventional manner according to the composition shown in Table 6.

Table 6

Compounding ingredient	Content (weight%)
Example	0.1
glycerin	3.0
Butylene glycol	2.0
Propylene glycol	2.0
Carboxy Vinyl Polymer	0.1
Fiji-12 nonylphenyl ether	0.2
Polysorbate 80	0.4
ethanol	10.0
Triethanolamine	0.1
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

[Formulation Example 2] nutrition lotion

To the nutritional lotion was prepared in a conventional manner according to the composition shown in Table 7.

TABLE 7

Compounding ingredient	Content (weight%)
Example	0.1
glycerin	3.0
Butylene glycol	3.0
Propylene glycol	3.0
Carboxy Vinyl Polymer	0.1
Beeswax	4.0
Polysorbate 60	1.5
Caprylic / Capric Triglycerides	5.0
Squalane	5.0
Sorbitassquioleate	1.5
Liquid paraffin	0.5
Cetearyl Alcohol	1.0
Triethanolamine	0.2
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

Formulation Example 3 Nutrition Cream

To the nutrition cream was prepared in a conventional manner according to the composition shown in Table 8.

Table 8

Compounding ingredient	Content (% by weight)
Example	0.1
glycerin	3.0
Butylene glycol	3.0
Liquid paraffin	7.0
Beta Glucan	7.0
Carbomer	0.1
Caprylic / Capric Triglycerides	3.0
Squalane	5.0
Cetearyl Glucoside	1.5
Sorbitan stearate	0.4
Polysorbate 60	1.2
Triethanolamine	0.1
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

Formulation Example 4 Massage Cream

To prepare a massage cream in a conventional manner according to the composition shown in Table 9.

Table 9

Compounding ingredient	Content (% by weight)
Example	0.1
glycerin	8.0
Butylene glycol	4.0
Liquid paraffin	45.0
Beta Glucan	7.0
Carbomer	0.1
Caprylic / Capric Triglycerides	3.0
Beeswax	4.0
Cetearyl Glucoside	1.5
Sesqui oleic acid sorbitan	0.9
Vaseline	3.0
paraffin	1.5
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

[Formulation Example 5] Pack

To prepare a pack in a conventional manner according to the composition described in Table 10 below.

Table 10

Compounding ingredient	Content (% by weight)
Example	0.1
glycerin	4.0
Polyvinyl alcohol	15.0
Hyaluronic acid extract	5.0
Beta Glucan	7.0
Allantoin	0.1
Nonyl Phenyl Ether	0.4
Polysorbate 60	1.2
ethanol	6.0
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

[Formulation Example 6] Ointment in the external preparation for skin

The ointment was prepared in a conventional manner according to the composition described in Table 11.

Table 11

Compounding ingredient	Content (% by weight)
Example	0.1
glycerin	8.0
Butylene glycol	4.0
Liquid paraffin	15.0
Beta Glucan	7.0
Carbomer	0.1
Caprylic / Capric Triglycerides	3.0
Squalane	1.0
Cetearyl Glucoside	1.5
Sorbitan stearate	0.4
Cetearyl Alcohol	1.0
Beeswax	4.0
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

Formulation Example 7 Preparation of Powder

Example ............... 100 mg

Lactose ......... 100 mg

Talc ........................ 10 mg

Maintenance ......................................... 5 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 8 Preparation of Tablet

Example ............... 50 mg

Corn starch ..... 100 mg

Lactose ............ 100 mg

Magnesium stearate .............. 2 mg

Vitamin C ........................ 50 mg

After mixing the above components and tableting according to the conventional tablet manufacturing method to prepare a tablet.

Formulation Example 9 Preparation of Capsule

Example ..................... 50 mg

Corn starch ..... 100 mg

Lactose ......................................... 100 mg

Magnesium Stearate ............... 2 mg

Vitamin C ............... 50 mg

Serine ............... 50 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 10 Preparation of Liquid

Example ......................................... 100 mg

Isomerized sugar ......................................... 10 g

Mannitol ............................................... 5 g

Vitamin C ............ 50 mg

Serine ... mg

maintain................................................. Quantity

Purified water ...............................

According to a conventional liquid preparation method, each component is added to the purified water to dissolve it, and then lemon flavor is added to the appropriate amount, and the above components are mixed. After adding purified water to adjust the whole to 100 ㎖ and then filled into a brown bottle to sterilize to prepare a liquid.

Formulation Example 11 Preparation of Health Food

Example ..................... 1000 mg

Vitamin mixtures

Vitamin A Acetate ............... 70 μg

Vitamin E ..................................... 1.0 mg

Vitamin B1 .................. 0.13 mg

Vitamin B2 ................................... 0.15 mg

Vitamin B6 ......................................... 0.5 mg

Vitamin B12 ................................. 0.2 μg

Vitamin C ........................................ 10 mg

Biotin ......................................... 10 μg

Nicotinic Acid Amide ... 1.7 mg

Folic acid ......................................... 50 μg

Calcium Pantothenate ..................... 0.5 mg

Mineral mixture

Ferrous Sulfate ............. 1.75 mg

Zinc Oxide ......................................... 0.82 mg

Magnesium Carbonate ... 25.3 mg

Potassium monophosphate ......................................... 15 mg

Dibasic Calcium Phosphate ......................................... 55 mg

Potassium Citrate ... 90 mg

Calcium Carbonate ... 100 mg

Magnesium Chloride ......................................... 24.8 mg

Although the composition ratio of the said vitamin and mineral mixture was mixed composition which is comparatively suitable for the health food in a preferable Example, you may change arbitrarily the compounding ratio. In addition, the above ingredients may be mixed according to a conventional health food manufacturing method, and then granules may be prepared and used to prepare a health food composition according to a conventional method.

Formulation Example 12 Preparation of Healthy Drink

Example ..................... 1000 mg

Citric Acid ......................................... 1000 mg

Oligosaccharide ......................................... 100 g

Plum concentrate ......................................... 2 g

Taurine ..................................... 1 g

Purified water is added to the whole .... 900 ml

The above ingredients are mixed according to a conventional healthy beverage preparation method, and then stirred and heated at 85 ° C. for about 1 hour. The resulting solution is filtered to obtain a sterilized 2 L container, sealed and sterilized and stored in cold storage to be used for the manufacture of a healthy beverage composition.

Although the composition ratio is composed of relatively suitable components for the preferred beverage in the preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as the demand class, the demand country, and the intended use.

Claims

As an active ingredient, a composition for preventing or improving skin wrinkles containing snowberry extract.
A composition for alleviating or inhibiting inflammation containing snowberry extract as an active ingredient.
A composition for skin whitening containing snowberry extract as an active ingredient.
A skin moisturizing composition containing a snowberry extract as an active ingredient.
Antioxidant composition containing a snowberry extract as an active ingredient.
Aging prevention or improvement composition containing a snowberry extract as an active ingredient.
The method according to any one of claims 1 to 6,

The snowberry composition comprising at least one selected from the group consisting of common snowberry, Mexican snowberry, little leaf snowberry, creeping snowberry, western snowberry, coralberry, mountain snowberry and Chinese coralberry .
The method according to any one of claims 1 to 6,

The snowberry extract composition is characterized in that it contains 0.1 to 30% by weight based on the total weight of the total composition.