WO2010123283A2 - Marqueur à base de cohésine marquée par fluorescence et procédé de détection d'enzymes incorporées dans les cellulosomes associé - Google Patents

Marqueur à base de cohésine marquée par fluorescence et procédé de détection d'enzymes incorporées dans les cellulosomes associé Download PDF

Info

Publication number
WO2010123283A2
WO2010123283A2 PCT/KR2010/002508 KR2010002508W WO2010123283A2 WO 2010123283 A2 WO2010123283 A2 WO 2010123283A2 KR 2010002508 W KR2010002508 W KR 2010002508W WO 2010123283 A2 WO2010123283 A2 WO 2010123283A2
Authority
WO
WIPO (PCT)
Prior art keywords
domain
protein
cohisine
cellulose
dockerine
Prior art date
Application number
PCT/KR2010/002508
Other languages
English (en)
Korean (ko)
Other versions
WO2010123283A3 (fr
WO2010123283A9 (fr
Inventor
한성옥
조우재
Original Assignee
고려대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 고려대학교 산학협력단 filed Critical 고려대학교 산학협력단
Publication of WO2010123283A2 publication Critical patent/WO2010123283A2/fr
Publication of WO2010123283A9 publication Critical patent/WO2010123283A9/fr
Publication of WO2010123283A3 publication Critical patent/WO2010123283A3/fr

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

La présente invention concerne un biomarqueur à base de cohésine et un procédé de détection d'enzymes incorporées dans les cellulosomes utilisant ledit marqueur. Plus spécifiquement, l'invention concerne un procédé permettant de détecter sélectivement uniquement les enzymes incorporées dans les cellulosomes en utilisant un biomarqueur comprenant la cohésine d'une protéine échafaudage dans la liaison entre le domaine de la cohésine de la protéine échafaudage et le domaine de la dockérine de l'enzyme incorporée dans les cellulosomes qui est un mécanisme de formation de cellulosome.
PCT/KR2010/002508 2009-04-24 2010-04-21 Marqueur à base de cohésine marquée par fluorescence et procédé de détection d'enzymes incorporées dans les cellulosomes associé WO2010123283A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20090035895 2009-04-24
KR10-2009-0035895 2009-04-24

Publications (3)

Publication Number Publication Date
WO2010123283A2 true WO2010123283A2 (fr) 2010-10-28
WO2010123283A9 WO2010123283A9 (fr) 2011-03-17
WO2010123283A3 WO2010123283A3 (fr) 2011-04-28

Family

ID=43011616

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/002508 WO2010123283A2 (fr) 2009-04-24 2010-04-21 Marqueur à base de cohésine marquée par fluorescence et procédé de détection d'enzymes incorporées dans les cellulosomes associé

Country Status (2)

Country Link
KR (1) KR101269596B1 (fr)
WO (1) WO2010123283A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111848758A (zh) * 2020-07-27 2020-10-30 齐鲁工业大学 一种适用于低钙离子浓度的纤维小体对接蛋白突变体及应用
CN111850005A (zh) * 2020-07-27 2020-10-30 齐鲁工业大学 一种适用于低钙离子浓度的纤维小体对接蛋白组合突变体36863及应用
CN111848759A (zh) * 2020-07-27 2020-10-30 齐鲁工业大学 一种活性提高的纤维小体对接蛋白突变体36741及应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101678067B1 (ko) * 2013-02-15 2016-11-21 고려대학교 산학협력단 클로스트리디움 셀룰로보란스 유래 엔도글루카나아제를 포함하는 셀룰로좀 복합체 및 그의 용도

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101821622B (zh) * 2007-08-17 2015-04-08 韩国科学技术院 分子相互作用的检测方法

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CARVALHO, A. ET AL. PROC. NATL. ACAD. SCI. USA vol. 100, no. 24, 2003, pages 13809 - 13814 *
CRAIG, S. ET AL. J. BIOTECHNOL. vol. 121, no. 2, 2006, pages 165 - 173 *
LYTLE, B. ET AL. J. BACTERIOL. vol. 180, no. 24, 1998, pages 6581 - 6585 *
MECHALY, A. ET AL. THE JOURNAL OF BIOLOGICAL CHEMISTRY vol. 276, no. 12, 2001, pages 9883 - 9888 *
PAGES. S. ET AL. PROTEINS vol. 29, no. 4, 1997, pages 517 - 527 *
YARON, S. ET AL. FEBS LETT. vol. 360, no. 2, 1995, pages 121 - 124 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111848758A (zh) * 2020-07-27 2020-10-30 齐鲁工业大学 一种适用于低钙离子浓度的纤维小体对接蛋白突变体及应用
CN111850005A (zh) * 2020-07-27 2020-10-30 齐鲁工业大学 一种适用于低钙离子浓度的纤维小体对接蛋白组合突变体36863及应用
CN111848759A (zh) * 2020-07-27 2020-10-30 齐鲁工业大学 一种活性提高的纤维小体对接蛋白突变体36741及应用
CN111848759B (zh) * 2020-07-27 2022-03-29 齐鲁工业大学 一种活性提高的纤维小体对接蛋白突变体36741及应用

Also Published As

Publication number Publication date
WO2010123283A3 (fr) 2011-04-28
KR20100117526A (ko) 2010-11-03
WO2010123283A9 (fr) 2011-03-17
KR101269596B1 (ko) 2013-06-05

Similar Documents

Publication Publication Date Title
Horne et al. Isolation and characterization of 5S RNA-protein complexes from Bacillus stearothermophilus and Escherichia coli ribosomes
Payne et al. Fungal cellulases
Shoemaker et al. Characterization and properties of cellulases purified from Trichoderma reesei strain L27
Kazemi‐Pour et al. The secretome of the plant pathogenic bacterium Erwinia chrysanthemi
Morlot et al. A highly coordinated cell wall degradation machine governs spore morphogenesis in Bacillus subtilis
Calvo et al. Analysis of the Listeria cell wall proteome by two‐dimensional nanoliquid chromatography coupled to mass spectrometry
US20060105442A1 (en) Promoters and proteins from Clostridium thermocellum and uses thereof
WO2010123283A2 (fr) Marqueur à base de cohésine marquée par fluorescence et procédé de détection d'enzymes incorporées dans les cellulosomes associé
WO2018208099A2 (fr) Vecteur recombinant porteur du domaine de liaison à la cellulose et procédé d'isolement et de purification de protéine utilisant ledit vecteur
Liu et al. CLR‐4, a novel conserved transcription factor for cellulase gene expression in ascomycete fungi
AU2018334254A1 (en) Function-based probes for environmental microbiome analysis and methods of making and using the same
Kolbe et al. Quantification and identification of the main components of the Trichoderma cellulase complex with monoclonal antibodies using an enzyme-linked immunosorbent assay (ELISA)
Maaty et al. Global analysis of viral infection in an archaeal model system
Wakarchuk et al. Proteomic analysis of the secretome of Cellulomonas fimi ATCC 484 and Cellulomonas flavigena ATCC 482
Blume et al. Characterization of Clostridium thermocellum isolates grown on cellulose and sugarcane bagasse
WO2003057138A2 (fr) Expression d'hydrolase lysosomiale dans des cellules exprimant pro-n-acetylglucosamine-1-phosphodiester alpha-n-acetyl glucosimanidase
Wu et al. Identification of glutaminyl cyclase genes involved in pyroglutamate modification of fungal lignocellulolytic enzymes
CN109321551B (zh) 一种新Trx-β-葡萄糖苷酶的基因及其蛋白表达、载体和应用
Hochuli et al. Interaction of hexahistidine fusion proteins with nitrilotriacetic acid-chelated Ni2+ ions
Sprey Complexity of cellulases from Trichoderma reesei with acidic isoelectric points: a two-dimensional gel electrophoretic study using immunoblotting
Franche et al. Cel5I, a SLH-containing glycoside hydrolase: characterization and investigation on its role in Ruminiclostridium cellulolyticum
US10435680B2 (en) Thermostable cellobiohydrolase
US20150017692A1 (en) Beta-glucosidase
US20150017690A1 (en) Beta-glucosidase
EP2995687B1 (fr) Beta-glucosidase thermostable

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10767297

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 10767297

Country of ref document: EP

Kind code of ref document: A2