WO2010115996A1 - Photobioréacteur dans un milieu fermé pour cultiver des microorganismes photosynthétiques - Google Patents

Photobioréacteur dans un milieu fermé pour cultiver des microorganismes photosynthétiques Download PDF

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Publication number
WO2010115996A1
WO2010115996A1 PCT/EP2010/054757 EP2010054757W WO2010115996A1 WO 2010115996 A1 WO2010115996 A1 WO 2010115996A1 EP 2010054757 W EP2010054757 W EP 2010054757W WO 2010115996 A1 WO2010115996 A1 WO 2010115996A1
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WIPO (PCT)
Prior art keywords
culture
light
elements
micro
enclosure
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PCT/EP2010/054757
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English (en)
Inventor
Michel Conin
Alain Friederich
Anthony Kerihuel
Luc Gerun
Gaël RUIZ
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Acta Alga
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Publication of WO2010115996A1 publication Critical patent/WO2010115996A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08—Air lift
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure

Definitions

  • the invention relates to intensive and continuous culture of micro-algae.
  • Micro-algae are photosynthetic plant organisms, the metabolism and growth of which require i.a. CO2, light and nutriments .
  • Micro-algae may be cultivated for exploiting and purifying carbon dioxide, NOx and/or SOx waste from certain factories (WO2008042919) .
  • Oil extracted from micro-algae may be used as a biofuel (WO2008070281, WO2008055190, WO2008060571) .
  • Micro-algae may be cultivated for their production of omega-3 and polyunsaturated fatty acids.
  • Micro-algae may also be cultivated for producing pigments .
  • a photobioreactor is defined as a closed system inside which biological interactions occur in the presence of light energy, which one attempts to control by mastering the culture conditions.
  • micro-algae Large scale industrial cultivation of micro-algae only uses the sun as a light source. For this, the micro- algae are often placed in open basins (raceways) with or without any flow (US2008178739) . Tubular or plate photobioreactors are also found, consisting of translucent materials, allowing passage of light rays into the culture medium in which the micro-algae circulate (FR2621323) . Other network systems of three-dimensional transparent tubes allow improvement in exploitation of the space (EP0874043) . These installations are extremely voluminous and production yields are low given the solar illumination random factors and night phases detrimental to the growth of micro-algae. In order to reduce bulkiness and improve yield, closed photobioreactors have been developed. They use as for them, the availability of artificial illumination 24 hours a day and 7 days a week. This illumination may be interrupted following sequences specific to the biological cycles of the relevant algae.
  • a first solution of artificial illumination for solving this problem consists of bringing light from a light source into the culture medium in proximity to the micro-algae by means of optical fibers (US 6,156,561 and EP 0935991) .
  • Optical fibers can be associated with other immersed means bringing light inside the enclosure (JP 2001/178443 and DE 29819259) .
  • Another artificial illumination solution for solving this problem consists of directly immerging light sources inside the enclosure of the photobioreactor, such as for example fluorescent lamps or LEDs (DE202007013406 and
  • immersed light sources in particular of LEDs
  • the first is inherent to the penetration of light into the culture, which is directly related to the density of the micro-algae. This density increases during the cultivation process and rapidly leads to extinction of the light flux in the major portion of the reactor.
  • the solutions consisting of illuminating the inner wall of the photobioreactor (DE202007013406) are therefore not transposable to industrial scale photobioreactors of several hundred liters by simple homothety, the absorption wavelength of the light always being centimetric at the end of the cultivation process.
  • the inventors have unexpectedly and surprisingly discovered a particular photobioreactor typology associating immersed light sources, a cooling system and a system for mixing the culture medium.
  • the object of the invention relates to a photobioreactor intended for cultivating photosynthetic micro-organisms, preferably micro-algae, comprising:
  • a culture enclosure (1) intended to contain the culture medium (3) of the micro-organisms, (b) in which light elements (2) are immersed, comprising light sources (7) placed in sealed enclosures (6) with adapted transparence (AT) so that the light sources (7) are isolated from the culture medium (3) .
  • A transparence
  • the enclosures with « adapted transparence » are enclosures which ensure an optimum optical yield in the wavelengths ensuring photosynthesis, this transparence may be adapted so as to take into account the heat transfer fluid which will circulate in the enclosure so that the successive diopters do not notably reduce the optical yield.
  • the culture enclosure (1) conventionally has a cylindrical or parallelepipedal shape.
  • the inner walls (27) of the culture enclosure (1) of the photobioreactor according to the invention are reflective so as to reduce at the very most, loss of light rays outside the closed enclosure. They may be covered with paint or a reflective material . Thus, the energy expenditure required for cultivating photosynthetic micro-organisms is reduced.
  • the cooling system consists of a heat transfer fluid (15) circulating in the sealed enclosures (6), said enclosures being connected to a cooling device exterior to the sealed enclosures for the heat transfer fluid (15) .
  • the heat transfer fluid (15) is selected for its transparence in the range of wavelengths from 0.3 micron to 1 micron and it should not have any significant absorption in this range of wavelengths.
  • Its optical index is selected by one skilled in the art in order to optimize coupling between the light sources (7) immersed therein and the other diopters located on the path of the light before reaching the culture medium.
  • the heat transfer fluid (15) circulates in the light elements (2), preferably in the direction of the height of the light element (2) and of the culture enclosure (1), i.e. from bottom to top and from top to bottom (see Fig. 4) . Either it circulates sideways between two light elements through a tube (16) or each light element has its own circulation.
  • the heat transfer fluid (15) is injected into the upper portion of the light elements, it circulates towards the bottom of the light elements and then towards another light element in which it circulates upwards.
  • the means for conveying the heat transfer fluid allow it to optimally circulate over the whole height of the light elements in order to cool all the light sources
  • the heat transfer fluid directly cools the light sources (7) by contact. It is itself directed towards and cooled by the cooling system of the photobioreactor of the invention, external to the culture enclosure (1) .
  • Heat control of this fluid further allows the culture enclosure to be thermostated.
  • the sealed enclosures (6) include two substantially parallel vertical walls (4) between which are placed the light sources (7) and additional walls (5) which will seal the enclosures.
  • the photobioreactor of the invention may further comprise a system for injecting gas (17) in particular CO 2 into the culture enclosure (1) .
  • the culture enclosure (1) of the photobioreactor according to the invention may be dimensioned for various industrial or laboratory applications.
  • the dimensions of a culture enclosure (1) at the scale of the laboratory are of a few tens of centimeters to a few hundred centimeters for the height and the diameter (cylindrical enclosure) or the width
  • the volume of the culture enclosure (1) at the scale of the laboratory is of at least one cubic meter (m 3 ) .
  • the culture enclosure (1) is an industrial culture enclosure (1) .
  • the dimensions of a culture enclosure (1) at an industrial scale is of several meters.
  • the volume of a culture enclosure (1) at an industrial scale is larger than one cubic meter.
  • the culture enclosure (1) is made in a material adapted for containing the culture medium, either metal or polymer for example, and preferentially selected from the group formed by PMMA, polycarbonate or stainless steel. Provision may also be made for enclosures in a construction material of the concrete type for example.
  • each location of the culture medium (3) present in the culture enclosure (1) of the photobioreactor according to the invention is found at less than 7 cm, preferably less than 5 cm, more preferably less than 3 cm from a light source.
  • a light source preferably less than 3 cm from a light source.
  • the production of biomass by the micro-algae is promoted by optimum, either continuous or pulsed illumination.
  • One skilled in the art will select the shape of the light elements (2), the number of light elements (2), the arrangement of the light elements (2), the number of light sources (7) per unit surface area in order to obtain this advantageous distance between each location of the culture medium (3) and a light source (7) and will optimally select the quality of the light elements.
  • a small unit volume of the culture medium (3) of less than one cubic millimeter (mm 3 ) is called a « location of the culture medium
  • the distance between both walls (4) of the light elements (2) of the photobioreactor of the invention is less than 15 cm, preferably 10 cm, more preferably 6 cm.
  • the light sources of the photobioreactor according to the invention are LEDs and/or OLEDs. These light sources are advantageous because they do not consume much energy. They may either illuminate continuously or with flashes. With them, it is possible to produce variable illumination sequences at will (illumination/extinction) .
  • the light modulation frequency may attain 100 kHertz.
  • this type of element also allows continuous control of the light intensity whether it is continuous or pulsed. They emit at one or several wavelengths. Advantageously, they emit at wavelengths corresponding to the chlorophyllian pigments.
  • each LED or OLED may have its own illumination sequence and be controlled in intensity. With the whole of these degrees of freedom, it will be possible to approach optimum culture conditions for the micro-organisms, which are different for each type of micro-organism.
  • the light sources (7) may be directly attached onto the internal face (10) of the sealed enclosures (6) of the light elements or else they may be attached onto arrays themselves attached in sealed enclosures (6) .
  • the density of the light sources (7) on the internal face of the light elements of the photobioreactor according to the invention or on the arrays is preferably from 1 to 40,000/m 2 , advantageously in order to obtain a completely illuminating surface.
  • the arrays of light sources (7) may be attached to the substantially parallel vertical walls (4) or to the additional walls (5) which will seal the enclosures (6) .
  • the arrays extend over the height of the light elements.
  • the arrays (18) are positioned at equal distance from each other, preferably at less than 10 cm, more preferably at less than 5 cm from each other.
  • the arrays of light sources may be positioned back to back, which provides hemispherical illumination.
  • the light emitted in an equatorial stratum directly penetrates the reactor. This stratum is limited in the vertical direction (top and bottom) by Brewster's incidence.
  • the reflected photons (beyond the Brewster angle) may advantageously be recycled by positioning reflection mirrors (x2) close to the light sources (top and bottom) .
  • reflection mirrors x2
  • planar light elements provision may be made for a reflection mirror all around the diode in order to reduce the illumination angle of the source and to thereby obtain a better produced light/transmitted
  • the mixing system (9) has two main functions; it should promote homogenization of the temperature of the culture medium on the one hand. On the other hand, it should allow homogenization of the illumination of the micro-organisms. Indeed, with this mixing, the micro-organisms pass from the illuminated areas to the non-illuminated areas and vice versa.
  • the mixing of the culture medium is achieved by various techniques; the most current technique presently is called an « air-lift Nurse Kinds of mechanical stirrings may also be used: Archimedes screw, marine propeller, of the Rushton type, hydrofoil, etc.
  • the mixing technique used is the one called « air lift » which consists of injecting a pressurized gas, for example air, into the low portion of the culture enclosure (1) .
  • the air with a lower density than the liquid, rapidly moves upwards as bubbles.
  • the liquid and the micro-algae are carried away by the upward movement of the bubbles.
  • the air injection may be carried out vertically but also obliquely in order to cause liquid to be transported from one wall to the other of the culture medium promoting mixing of the nutriments and of CO2 necessary for the micro-algae.
  • This movement of the culture liquid also ensures a mean illumination to all the micro-algae during their upward motion.
  • the micro- algae then fall back into the volumes where there is no upward motion of air bubbles. In this way, a closed travel loop of the culture medium has been achieved.
  • This technique allows mixing with low energy consumption and slightly stressing for the micro-algae.
  • the mixing of the culture medium may be partly achieved by a standard air-lift system, which essentially imparts a vertical pulse, completed by an original system of lateral injection (CO2 + air) distributed with « feeders » (30) over the height of the reactor (tube or plates) .
  • « Feeder » designates here a duct or tube allowing gas or water to be transported from the source right up to the location where injection of gas or water is desired.
  • Said « feeders » (30) will be set up in the culture area against the walls (4) of the light elements
  • the injection nozzles (29) are distributed on « feeder (s) » (30) .
  • Their number as well as their inclination will depend on the type of pulse which one wishes to impart to the micro-organisms (transverse pulse, vertical pulse or pulse) allowing a global movement to be imparted to the biomass, with which the algae may periodically move from one edge to the other of the reactor, with an upward movement.
  • this capacity for handling the transverse movement of the biomass will be used for homogenizing the illumination of the latter, i.e. preferentially oriented upwards with a specific inclination.
  • a volume of culture is taken regularly or continuously, which is straightaway replaced by the injection of an equivalent volume of water containing nutritious elements in the low portion of the culture enclosure (1) or in the « feeder (s) » (30) .
  • this method it is possible to contribute to the reduction of the energy required for inducing circulation of the liquid in the reactor.
  • the currents of liquids induced by the various injections of air or water in the feeders (30) cyclically transport the algae in proximity to the light elements (2) and around the light elements (2) .
  • the cooling system (8) allows extraction of the heat released by the light sources (7) while adjusting the temperature of the culture medium (3) of the photobioreactor (see Figs. 3 and 4) . It also allows circulation of the heat transfer fluid (15) in the light elements (2) .
  • the cooling system (8) may consist in a heat exchanger.
  • this heat exchanger consists in means for conveying (19) the hot heat transfer liquid (15) towards the outside of the culture enclosure (1), for example pipes connected to the upper end of the culture enclosure (1) coupled with a pump (20) and a cooler (21) consisting of circulating the hot heat transfer liquid in the opposite direction to the cold water (see Fig. 4) .
  • the heat transfer liquid (15) flows out of the culture enclosure (1) at one of its ends, at the top or at the bottom and enters the culture enclosure (1) through the other end.
  • the cold heat transfer liquid (15) returns into the culture enclosure (1) via means for conveying it (22), for example pipes.
  • All the light elements (2) of the photobioreactor of the invention may have an identical shape or a different shape.
  • All the light elements (2) of the photobioreactor of the invention may have identical or different dimensions.
  • the light elements of the photobioreactor of the invention may appear in many forms insofar that they extend approximately over the whole height of the culture enclosure (1) and insofar that they may be connected through one end of the culture enclosure (1) to the cooling system.
  • They may have a parallelepipedal shape (14) . They then have the advantage of being easy to clean. They then comprise two plates with adapted transparence corresponding to the walls (4), one of the sides of which has a length approximately equal to the height of the culture enclosure (1) . These plates are attached to each other through other walls (5) with adapted transparence which will seal the thereby formed parallelepiped filled with heat transfer liquid (15) (see Fig. 5) . The two plates of material with suitable transparence are then distant from each other by less than 10 cm, preferably less than 8 cm, more preferably less than 6 cm.
  • the light sources (7) are preferably attached onto the walls (4) or (5) and positioned at equal distance from the plates.
  • the external face (24) of the plates is in contact with the culture medium (3) .
  • the mixing system (9) by « air lift » consists of injecting air between the plates in the low portion of the culture enclosure, preferably at equal distance from the two plates, each injection system being separated by at least 15 cm, preferably 10 cm, more preferably 6 cm.
  • the light elements may also for example appear as hollow cylinders (11) (see Figs. 1 and 2) . They then comprise two tubes (12) with adapted transparence
  • both tubes (12) (corresponding to the walls (4)) having two different diameters, fitted into each other.
  • the difference between both diameters of both tubes (12) is of less than 10 cm, preferably less than 6 cm, more preferably less than 3 cm.
  • Both of these tubes (12) are attached to each other through other walls (5) , preferably with adapted transparence, which will seal off the space between the tubes filled with heat transfer fluid (15) (see Fig. 5) .
  • the light sources (7) are attached on the faces (25) of the tubes (12) or at equal distance from the faces (25) of the tubes (12) .
  • another cylindrical light element (13) may be added along the axis of the hollow cylinders (11) (see Fig. 1) .
  • Light sources (7) are then placed in its centre in order to thereby optimize the illumination of the micro-algae.
  • the height of the cylinders is approximately equal to the height of the culture enclosure (1) while the height of the hollow cylinders is smaller than that of the cylinders .
  • the light elements may also appear as cylinders (26), their height being approximately equal to the height of the culture enclosure (1) . Their diameter is of less than 20 cm, preferably less than 10 cm, more preferably less than 5 cm. They are filled with heat transfer liquid.
  • the light sources (7) are placed in its centre.
  • the light elements of the three preceding embodiments may be combined, i.e. light elements according to the three preceding embodiments may be present in the culture enclosure (1) of the photobioreactor according to the invention.
  • the suitable materials with adapted transparence for making the light elements (2) are PMMA (methyl polymethacrylate) , Plexiglas®, glass, polycarbonate, PMMA plates, with or without light diffusers which allow homogenization of the point-like light sources.
  • Suitable heat transfer fluids are silicon oil, perfluorinated oil or air.
  • perfluorinated oil is used as a heat transfer fluid (15), which has the following advantages: it is chemically inert towards the arrays of photodiodes,
  • Another object of the invention is the use of a photobioreactor according to the invention for cultivating photosynthetic micro-organisms, preferably micro-algae .
  • FIG. 1 Diagram of the principle of the cylindrical photobioreactor .
  • Fig. 2 Diagram of the cylindrical photobioreactor with light elements as a hollow cylinder and as a cylinder.
  • Figs . 3 and 4 Presentation of the cooling system of the light source and of the system for controlling the temperature of the photobioreactor.
  • Fig. 5 Diagram of a light element as a hollow plate (14) .
  • Fig. 6 Diagram of a light element as a hollow cylinder and as a cylinder.
  • Fig. 7 Diagram of the parallelepipedal photobioreactor with light elements as a hollow plate.
  • Fig. 8 Diagram of a light element as a hollow plate with feeders (30) and nozzles (29) .
  • Fig. 9 Zoom of Fig. 8.
  • the light elements and the reactor are parallelepipedal.
  • the light elements then consist in two plates (4) with adapted transparence attached to each other through other walls with adapted transparence (5) .
  • Both plates have a height smaller than the height of the culture enclosure (1) and a length comprised between 25 and 65 cm. Both plates are distant by 5 cm.
  • the light sources (7) are LEDs. They are attached on arrays positioned back to back at the centre of the plates in an amount of at least 300 LEDs/m 2 .
  • the light elements are positioned so that their length and their width are parallel to the length and the width of the culture enclosure (1) .
  • the light elements are distant from each other by 6 cm.
  • the mixing system (9) by « air lift » consists in injecting air in air injection points (28) located at an equal distance from two plates, at the bottom of the culture enclosure
  • each injection point (28) being distant by 12 cm.
  • the tubes (12) comprise two tubes (12) with adapted transparence having a diameter of 15 cm and a diameter of 20 cm, fitted into each other, the height of the tubes (12) being approximately equal to the height of the culture enclosure (1) .
  • Both of the tubes (12) are attached to each other through other walls (5) .
  • the light sources (7) are LEDs. They are attached on arrays (18) in an amount of at least 140 LEDs/m 2 .
  • the light arrays (18) are positioned back to back between both tubes at an equal distance from each of them.
  • the light arrays (18) are uniformly distributed over the circumference of the space between the tubes.
  • Another light element as a cylinder
  • each light element is present in the centre of each light element as a hollow cylinder (11) .
  • the light elements as a hollow cylinder (11) have a smaller height than that of the light elements as a cylinder (12) .
  • LEDs are also attached to the centre of the cylindrical light element (12) in an amount of at least 140 LEDs/m 2 .
  • the mixing system (9) by « air-lift » consists of injecting air into the low portion of the culture enclosure (1) .

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  • Life Sciences & Earth Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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Abstract

L'invention porte sur un photobioréacteur destiné à cultiver des microorganismes photosynthétiques, comprenant : (a) une enceinte de culture (1) destinée à contenir le milieu de culture (3) des microorganismes, (b) dans laquelle des éléments lumineux (2) sont immergés comprenant des sources de lumière (7) placées dans des enceintes étanches (6) présentant une transparence adaptée (AT) de telle sorte que les sources de lumière (7) sont isolées du milieu de culture (3), (c) un système (8) pour refroidir les sources de lumière (7) et (d) un système (9) pour mélanger le milieu de culture (3).
PCT/EP2010/054757 2009-04-10 2010-04-12 Photobioréacteur dans un milieu fermé pour cultiver des microorganismes photosynthétiques WO2010115996A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0952391A FR2944291B1 (fr) 2009-04-10 2009-04-10 Photobioreacteur en milieu ferme pour la culture de micro-organismes photosynthetiques
FR0952391 2009-04-10

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EP2459693A2 (fr) * 2009-07-30 2012-06-06 Tendris Solutions B.V. Réacteur à algues
WO2012098031A1 (fr) * 2011-01-17 2012-07-26 Wacker Chemie Ag Photobioréacteur éclairé au moyen de pièces moulées luminescentes
WO2013079948A1 (fr) * 2011-11-29 2013-06-06 Xanthella Limited Photobioréacteur
WO2013063075A3 (fr) * 2011-10-24 2013-07-11 Heliae Development Llc Systèmes et procédés permettant de faire croître des organismes photosynthétiques
WO2014108665A1 (fr) * 2013-01-09 2014-07-17 Industrial Phycology Limited Photobioréacteur
NL2012157C2 (en) * 2014-01-28 2015-07-29 Photanol B V Arrangement of a photobioreactor or a microbiological reactor.
US9200236B2 (en) 2011-11-17 2015-12-01 Heliae Development, Llc Omega 7 rich compositions and methods of isolating omega 7 fatty acids
CN108929843A (zh) * 2018-07-23 2018-12-04 河南农业大学 一种生物质光合细菌制氢装置
US20190144806A1 (en) * 2012-03-16 2019-05-16 Forelight, Inc. Methods and materials for cultivation and/or propagation of a photosynthetic organism
CN110240998A (zh) * 2018-03-07 2019-09-17 曾坚 自动清刷管壁的列管式密闭光生物反应器
FR3095210A1 (fr) * 2019-04-21 2020-10-23 Dominique Delobel Système de production de micro organismes photosynthétiques
CN112063519A (zh) * 2020-09-14 2020-12-11 中国科学院重庆绿色智能技术研究院 一种光合细菌连续培养装置及其控制方法
WO2021149045A1 (fr) * 2020-01-20 2021-07-29 Brevel Ltd. Photobioréacteurs fermés pour la culture de micro-organismes
CN113583809A (zh) * 2021-08-26 2021-11-02 德州六顺电气自动化设备有限公司 一种基于微藻闪光效应的反应器补光器及其补光方法
EP3904493A1 (fr) * 2020-04-30 2021-11-03 O&N B.V. Photobioréacteur
US11214767B2 (en) 2020-05-22 2022-01-04 Brightwave Partners, LLC Internally illuminated bioreactor

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CN113302275A (zh) * 2019-01-15 2021-08-24 叶玛亚有限公司 可扩展的光合微生物生产和培养系统
FR3098828B1 (fr) 2019-07-19 2021-08-27 Centralesupelec Dispositif et procédé de production de microorganismes photosynthétiques en photobioréacteur

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CN108929843A (zh) * 2018-07-23 2018-12-04 河南农业大学 一种生物质光合细菌制氢装置
CN108929843B (zh) * 2018-07-23 2024-06-04 河南农业大学 一种生物质光合细菌制氢装置
FR3095210A1 (fr) * 2019-04-21 2020-10-23 Dominique Delobel Système de production de micro organismes photosynthétiques
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CN112063519B (zh) * 2020-09-14 2022-06-10 中国科学院重庆绿色智能技术研究院 一种光合细菌连续培养装置及其控制方法
CN113583809A (zh) * 2021-08-26 2021-11-02 德州六顺电气自动化设备有限公司 一种基于微藻闪光效应的反应器补光器及其补光方法
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