WO2010099414A3 - Protection of human corneal endothelial against cell death under storage conditions by anti-apoptotic genes - Google Patents

Protection of human corneal endothelial against cell death under storage conditions by anti-apoptotic genes Download PDF

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Publication number
WO2010099414A3
WO2010099414A3 PCT/US2010/025542 US2010025542W WO2010099414A3 WO 2010099414 A3 WO2010099414 A3 WO 2010099414A3 US 2010025542 W US2010025542 W US 2010025542W WO 2010099414 A3 WO2010099414 A3 WO 2010099414A3
Authority
WO
WIPO (PCT)
Prior art keywords
protection
cell death
storage conditions
corneal endothelial
under storage
Prior art date
Application number
PCT/US2010/025542
Other languages
French (fr)
Other versions
WO2010099414A2 (en
Inventor
Thomas Fuchsluger
Andrius Kazlauskas
Reza Dana
Original Assignee
Universitat Duisburg-Essen
Schepens Eye Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitat Duisburg-Essen, Schepens Eye Research Institute filed Critical Universitat Duisburg-Essen
Publication of WO2010099414A2 publication Critical patent/WO2010099414A2/en
Publication of WO2010099414A3 publication Critical patent/WO2010099414A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The invention relates to methods of modifying endothelial cells of corneal tissue to express an anti-apoptotic gene, to prevent endothelial cell loss during cornea storage and to improve the quality of cornea tissue prior to transplantation. The invention also relates to a kit for cornea storage comprising a media, an anti- apoptotic gene and a vector.
PCT/US2010/025542 2009-02-27 2010-02-26 Protection of human corneal endothelial against cell death under storage conditions by anti-apoptotic genes WO2010099414A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15593409P 2009-02-27 2009-02-27
US61/155,934 2009-02-27

Publications (2)

Publication Number Publication Date
WO2010099414A2 WO2010099414A2 (en) 2010-09-02
WO2010099414A3 true WO2010099414A3 (en) 2010-10-21

Family

ID=42352252

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/025542 WO2010099414A2 (en) 2009-02-27 2010-02-26 Protection of human corneal endothelial against cell death under storage conditions by anti-apoptotic genes

Country Status (1)

Country Link
WO (1) WO2010099414A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020056392A1 (en) 2018-09-14 2020-03-19 University Of Miami Dual-chamber vial for corneal graft preservation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020068050A1 (en) * 2000-10-11 2002-06-06 Williams Keryn Anne Corneal cells expressing active agents and methods of use thereof
WO2006110552A2 (en) * 2005-04-12 2006-10-19 Cleo Cosmetic And Pharmaceutical Co., Llc Composition and method for in vitro preservation of corneal tissues

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020068050A1 (en) * 2000-10-11 2002-06-06 Williams Keryn Anne Corneal cells expressing active agents and methods of use thereof
WO2006110552A2 (en) * 2005-04-12 2006-10-19 Cleo Cosmetic And Pharmaceutical Co., Llc Composition and method for in vitro preservation of corneal tissues

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BARCIA R N ET AL: "Corneal graft rejection is accompanied by apoptosis of the endothelium and is prevented by gene therapy with bcl-xL.", AMERICAN JOURNAL OF TRANSPLANTATION : OFFICIAL JOURNAL OF THE AMERICAN SOCIETY OF TRANSPLANTATION AND THE AMERICAN SOCIETY OF TRANSPLANT SURGEONS SEP 2007 LNKD- PUBMED:17614980, vol. 7, no. 9, September 2007 (2007-09-01), pages 2082 - 2089, XP002596223, ISSN: 1600-6135 *
BARCIA R N ET AL: "Preventing apoptosis in corneal endothelial cells", IOVS, vol. 46, no. Suppl. S, 2005, & ANNUAL MEETING OF THE ASSOCIATION-FOR-RESEARCH-IN-VISION-AND-OPHTHALM OLOGY; FT LAUDERDALE, FL, USA; MAY 01 -05, 2005, pages 4775, XP002596222, ISSN: 0146-0404 *
GAIN PHILIPPE ET AL: "In situ immunohistochemical study of Bcl-2 and heat shock proteins in human corneal endothelial cells during corneal storage", BRITISH JOURNAL OF OPHTHALMOLOGY, vol. 85, no. 8, August 2001 (2001-08-01), pages 996 - 1000, XP002596224, ISSN: 0007-1161 *
GONG ET AL: "Gene transfer of cyto-protective molecules in corneal endothelial cells and cultured corneas: Analysis of protective effects in vitro and in vivo", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 357, no. 1, 18 April 2007 (2007-04-18), pages 302 - 307, XP022029966, ISSN: 0006-291X *
JOO C-K ET AL: "PROTECTIVE ROLE OF BCL-2 IN EXPERIMENTALLY INDUCED CELL DEATH OF BOVINE CORNEAL ENDOTHELIAL CELLS", OPHTHALMIC RESEARCH, KARGER, CH LNKD- DOI:10.1159/000055549, vol. 31, no. 4, 1 July 1999 (1999-07-01), pages 287 - 296, XP008013602, ISSN: 0030-3747 *
QIAN YING ET AL: "Ex vivo adenovirus-mediated gene transfer to corneal graft endothelial cells in mice.", INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE JUL 2004 LNKD- PUBMED:15223794, vol. 45, no. 7, July 2004 (2004-07-01), pages 2187 - 2193, XP002596225, ISSN: 0146-0404 *

Also Published As

Publication number Publication date
WO2010099414A2 (en) 2010-09-02

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