WO2010088872A3 - Biosynthesis and obtaining substances from cells - Google Patents

Biosynthesis and obtaining substances from cells Download PDF

Info

Publication number
WO2010088872A3
WO2010088872A3 PCT/DE2010/000048 DE2010000048W WO2010088872A3 WO 2010088872 A3 WO2010088872 A3 WO 2010088872A3 DE 2010000048 W DE2010000048 W DE 2010000048W WO 2010088872 A3 WO2010088872 A3 WO 2010088872A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
substances
biosynthesis
obtaining
obtaining substances
Prior art date
Application number
PCT/DE2010/000048
Other languages
German (de)
French (fr)
Other versions
WO2010088872A2 (en
Inventor
Edeltraud Mast-Gerlach
Ulf Stahl
Dietrich Knorr
Dennis Raschke
Original Assignee
Vlb Berlin E.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vlb Berlin E.V. filed Critical Vlb Berlin E.V.
Priority to EP10715485A priority Critical patent/EP2393908A2/en
Publication of WO2010088872A2 publication Critical patent/WO2010088872A2/en
Publication of WO2010088872A3 publication Critical patent/WO2010088872A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C11B1/108—Production of fats or fatty oils from raw materials by extracting after-treatment, e.g. of miscellae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

The invention relates to a method for biosynthesizing and obtaining substances from cells. Fields of application of the invention include industry, in particular the food industry, but also all other branches, medicine and the pharmaceutical industry. The method according to the invention for obtaining substances from cells is characterized in that the cells producing the desired substances are cultivated and the formed substances are released from the cells or made accessible for extraction by treating the culture medium with high voltage pulses and are subsequently extracted from the medium.
PCT/DE2010/000048 2009-02-04 2010-01-20 Biosynthesis and obtaining substances from cells WO2010088872A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10715485A EP2393908A2 (en) 2009-02-04 2010-01-20 Biosynthesis and obtaining substances from cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009007402.3 2009-02-04
DE102009007402A DE102009007402A1 (en) 2009-02-04 2009-02-04 Biosynthesis and extraction of substances from cells

Publications (2)

Publication Number Publication Date
WO2010088872A2 WO2010088872A2 (en) 2010-08-12
WO2010088872A3 true WO2010088872A3 (en) 2010-11-18

Family

ID=42308992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2010/000048 WO2010088872A2 (en) 2009-02-04 2010-01-20 Biosynthesis and obtaining substances from cells

Country Status (3)

Country Link
EP (1) EP2393908A2 (en)
DE (1) DE102009007402A1 (en)
WO (1) WO2010088872A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2305603A1 (en) * 1973-02-05 1974-08-08 Mario Menegatto Extraction of fats, proteins and enzymes - by emulsifying with shock waves, aq mixts. of animal or vegetable matter at low temp
US3958027A (en) * 1974-06-14 1976-05-18 Simon-Rosedowns Limited Extraction
CA2055390A1 (en) * 1990-11-15 1992-05-16 Jocelyn J.R. Pare Microwave assisted process for extraction and apparatus therefor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2305603A1 (en) * 1973-02-05 1974-08-08 Mario Menegatto Extraction of fats, proteins and enzymes - by emulsifying with shock waves, aq mixts. of animal or vegetable matter at low temp
US3958027A (en) * 1974-06-14 1976-05-18 Simon-Rosedowns Limited Extraction
CA2055390A1 (en) * 1990-11-15 1992-05-16 Jocelyn J.R. Pare Microwave assisted process for extraction and apparatus therefor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LAKKAKULA N R ET AL: "Rice bran stabilization and rice bran oil extraction using ohmic heating", BIORESOURCE TECHNOLOGY, vol. 92, no. 2, April 2004 (2004-04-01), pages 157 - 161, XP002594234, DOI: DOI:10.1016/J.BIORTECH.2003.08.010 *

Also Published As

Publication number Publication date
EP2393908A2 (en) 2011-12-14
WO2010088872A2 (en) 2010-08-12
DE102009007402A1 (en) 2010-08-05

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