WO2010082071A3 - Micropropagation method and apparatus - Google Patents

Micropropagation method and apparatus Download PDF

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Publication number
WO2010082071A3
WO2010082071A3 PCT/HU2010/000005 HU2010000005W WO2010082071A3 WO 2010082071 A3 WO2010082071 A3 WO 2010082071A3 HU 2010000005 W HU2010000005 W HU 2010000005W WO 2010082071 A3 WO2010082071 A3 WO 2010082071A3
Authority
WO
WIPO (PCT)
Prior art keywords
culture medium
liquid culture
bioreactor
micropropagation
medium container
Prior art date
Application number
PCT/HU2010/000005
Other languages
French (fr)
Other versions
WO2010082071A2 (en
Inventor
Miklόs Gábor FÁRI
Tamás KERTÉSZ
Ferenc Csaba Paluska
Original Assignee
Interest-Trade
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Interest-Trade filed Critical Interest-Trade
Publication of WO2010082071A2 publication Critical patent/WO2010082071A2/en
Publication of WO2010082071A3 publication Critical patent/WO2010082071A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A01G31/02—Special apparatus therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02—Percolation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The invention relates to a micropropagation method, wherein a bioreactor (1) and a liquid culture medium container (2) are applied, being in communicating connection with each other via a connecting tube (3a) which enables the flow of liquid culture medium. A further connecting tube (3b) is also used to establish a communicating connection between the headspace atmospheres of the bioreactor (1) and the liquid culture medium container (2), and the amount of liquid culture medium in the bioreactor (1) is regulated by changing the interrelated vertical positions of the liquid culture medium container (2) and the bioreactor (1). The invention also relates to a micropropagation apparatus, for carrying out the method.
PCT/HU2010/000005 2009-01-15 2010-01-13 Micropropagation method and apparatus WO2010082071A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU0900018A HUP0900018A2 (en) 2009-01-15 2009-01-15 Method and device for micropropagation
HUP0900018 2009-01-15

Publications (2)

Publication Number Publication Date
WO2010082071A2 WO2010082071A2 (en) 2010-07-22
WO2010082071A3 true WO2010082071A3 (en) 2010-12-16

Family

ID=89988711

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU2010/000005 WO2010082071A2 (en) 2009-01-15 2010-01-13 Micropropagation method and apparatus

Country Status (2)

Country Link
HU (2) HU4465U (en)
WO (1) WO2010082071A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE1019588A5 (en) * 2011-05-18 2012-08-07 Sopet Nv CONTAINER SYSTEM FOR DIP GROWTH REGIME.
CN102613081B (en) * 2012-04-01 2013-09-18 广州甘蔗糖业研究所 Device and method for rapidly propagating sugarcane tissue-culture seedlings by changing culture solution in system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4419842A (en) * 1982-02-08 1983-12-13 Michael Paloian Hydroponic planter
EP0142989A2 (en) * 1983-11-17 1985-05-29 Applied Aeroponics, Inc. Plant propagation system and apparatus
EP0308545A1 (en) * 1987-09-25 1989-03-29 Augustinus Gerardus Maria Vogels A method and an apparatus for automatically watering plants
US5558984A (en) * 1994-06-03 1996-09-24 Clemson University Automated system and process for heterotrophic growth of plant tissue

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4669217A (en) 1984-11-17 1987-06-02 Aeroponics, Associates-1983 Ltd. Plant propagation system and apparatus
JP2000041166A (en) 1998-07-23 2000-02-08 Minolta Co Ltd Digital camera
US6753178B2 (en) 2000-03-01 2004-06-22 Clemson University Intermittent immersion vessel apparatus and process for plant propagation
HUP0401841A2 (en) 2004-09-10 2006-07-28 Agroinvest Kuelkereskedelmi Combined micropropagating process and bioreactor

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4419842A (en) * 1982-02-08 1983-12-13 Michael Paloian Hydroponic planter
EP0142989A2 (en) * 1983-11-17 1985-05-29 Applied Aeroponics, Inc. Plant propagation system and apparatus
EP0308545A1 (en) * 1987-09-25 1989-03-29 Augustinus Gerardus Maria Vogels A method and an apparatus for automatically watering plants
US5558984A (en) * 1994-06-03 1996-09-24 Clemson University Automated system and process for heterotrophic growth of plant tissue

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SAYED M.A. ZOBAYED ET AL: "evolution of culture vessel for micropropagation from test tube to culture room", TRANSPLANT PRODUCTION IN THE 21 ST CENTURY, 2000, netherlands, pages 231 - 237, XP002606273, Retrieved from the Internet <URL:http://www.h.chiba-u.ac.jp/kanko/paper/2005/DATA/1999to2002/626.pdf> [retrieved on 20101021] *

Also Published As

Publication number Publication date
HUP0900018A2 (en) 2010-09-28
WO2010082071A2 (en) 2010-07-22
HU4465U (en) 2014-12-29
HU0900018D0 (en) 2009-03-30

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