WO2010080978A3 - Pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components - Google Patents

Pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components Download PDF

Info

Publication number
WO2010080978A3
WO2010080978A3 PCT/US2010/020470 US2010020470W WO2010080978A3 WO 2010080978 A3 WO2010080978 A3 WO 2010080978A3 US 2010020470 W US2010020470 W US 2010020470W WO 2010080978 A3 WO2010080978 A3 WO 2010080978A3
Authority
WO
WIPO (PCT)
Prior art keywords
whole blood
leukocytes
blood sample
depletion
capture
Prior art date
Application number
PCT/US2010/020470
Other languages
French (fr)
Other versions
WO2010080978A2 (en
Inventor
Sunitha Nagrath
Mehmet Toner
Original Assignee
The General Hospital Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The General Hospital Corporation filed Critical The General Hospital Corporation
Publication of WO2010080978A2 publication Critical patent/WO2010080978A2/en
Publication of WO2010080978A3 publication Critical patent/WO2010080978A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons

Abstract

Methods, apparatuses, and microfluidic systems for pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components is described. A microfluidic device is used to pre-deplete the leukocytes in a whole blood sample. To do so, parallel micro-channels in the microfluidic device are treated with binding moieties to bind leukocytes but not target cells. When the whole blood sample is flowed through the microfluidic device, it is depleted of the leukocytes to form a depleted sample. The depleted sample is then flowed through a second micro-channel treated to bind target cells.
PCT/US2010/020470 2009-01-08 2010-01-08 Pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components WO2010080978A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14331609P 2009-01-08 2009-01-08
US61/143,316 2009-01-08

Publications (2)

Publication Number Publication Date
WO2010080978A2 WO2010080978A2 (en) 2010-07-15
WO2010080978A3 true WO2010080978A3 (en) 2010-11-18

Family

ID=42317153

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/020470 WO2010080978A2 (en) 2009-01-08 2010-01-08 Pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components

Country Status (1)

Country Link
WO (1) WO2010080978A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6991192B2 (en) 2016-06-21 2022-01-12 クァンタムディーエックス グループ リミテッド Improving Target Cell Concentration Using Dielectrophoresis (DEP)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103119441A (en) * 2010-08-15 2013-05-22 Gpb科学有限责任公司 Microfluidic cell separation in the assay of blood
SG11201406767VA (en) * 2012-04-20 2014-12-30 Agency Science Tech & Res Microfilter and apparatus for separating a biological entity from a sample volume
SG10201705895TA (en) * 2013-01-24 2017-08-30 Nat Univ Singapore Microdevices for separation of non-spherical particles and applications thereof
WO2016059302A1 (en) * 2014-10-17 2016-04-21 Ecole Polytechnique Method for handling microdrops which include samples
CN104483482A (en) * 2014-12-22 2015-04-01 常熟市创新模具有限公司 Method for manufacturing device used for detecting tumor cells
CN104483483A (en) * 2014-12-22 2015-04-01 常熟市创新模具有限公司 Device for detecting tumor cells
CN104560714B (en) * 2015-01-31 2016-09-07 重庆大学 For cultivating and detect the micro-fluidic chip of lung carcinoma cell
CN104560715B (en) * 2015-01-31 2016-09-07 重庆大学 Cultivate based on micro-fluidic chip and the method for detection lung carcinoma cell
US10774393B2 (en) 2016-05-20 2020-09-15 Roche Molecular Systems, Inc. Cell surface marker depletion in a sample processing device
CN105950469B (en) * 2016-06-08 2018-03-06 牛海涛 Cell screening chip and micro-fluidic chip joint
CN106350439A (en) * 2016-11-10 2017-01-25 上海美吉逾华生物医药科技有限公司 Micro-fluidic chip for cell capture and fluorescent staining

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007106598A2 (en) * 2006-03-15 2007-09-20 The General Hospital Corporation Devices and methods for detecting cells and other analytes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007106598A2 (en) * 2006-03-15 2007-09-20 The General Hospital Corporation Devices and methods for detecting cells and other analytes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ADAMS, A. A. ET AL.: "High Efficient Circulating Tumor Cell Isolation from Whole Blood and Label-Free Enumeration Using Polymer-Based Microfluidics with an Integrated Conductivity Sensor", J. AM. CHEM. SOC., vol. 130, no. 27, July 2008 (2008-07-01), pages 8633 - 8641 *
NAGRATH, S. ET AL.: "Isolation of Rare Circulating Tumour Cells in Cancer Patients by Microchip Technology", NATURE. DEC., vol. 450, no. 7173, 2007, pages 1235 - 1239 *
SOOHOO, J. ET AL.: "Microfluidic Liquid Filters for Leukocytes Isolation", CONF. PROC. IEEE ENG. MED. BIOL. SOC., 2007, pages 6319 - 6322 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6991192B2 (en) 2016-06-21 2022-01-12 クァンタムディーエックス グループ リミテッド Improving Target Cell Concentration Using Dielectrophoresis (DEP)

Also Published As

Publication number Publication date
WO2010080978A2 (en) 2010-07-15

Similar Documents

Publication Publication Date Title
WO2010080978A3 (en) Pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components
WO2011100708A3 (en) Assay card for sample acquisition, treatment and reaction
WO2012100235A3 (en) Systems and methods for sample use maximization
WO2011063416A3 (en) Microfluidic devices for the capture of biological sample components
WO2010124227A3 (en) Methods and devices for capturing circulating tumor cells
WO2012094642A3 (en) Circulating tumor cell capture on a microfluidic chip incorporating both affinity and size
WO2009121032A3 (en) Sample preparation devices and methods for processing analytes
MX363916B (en) SYSTEMS and DEVICES FOR ANALYSIS OF SAMPLES.
EP2297586A4 (en) Cartridge containing reagent, microfluidic device including the cartridge, method of manufacturing the microfluidic device, and biochemical analysis method using the microfluidic device
WO2009117611A3 (en) Trapping magnetic cell sorting system
WO2010091414A3 (en) Improvements in and relating to components of microfluidic devices
EP2315848A4 (en) Methods and systems for microfluidic dna sample preparation
WO2009053928A3 (en) Cartridge for a biological sample
WO2012045012A3 (en) Sandwich assays in droplets
WO2014028378A3 (en) Methods and systems for detecting biological components
WO2010123594A3 (en) Device for filtration of fluids there through and accompanying method
EP2329276A4 (en) Centrifugal-based microfluid apparatus, method of fabricationg the same, and method of testing samples using the microfluidic apparatus
WO2011088014A3 (en) Systems and methods for counting cells and biomolecules
EP2255183A4 (en) Assay preparation plates, fluid assay preparation and analysis systems, and methods for preparing and analyzing assays
WO2009051821A3 (en) Sample preparation devices and analyzers
WO2012057498A3 (en) Cell collection device
WO2008068215A3 (en) Device and method for analyzing body fluids
EP2772761A3 (en) Nucleic acid element for use in analysis, and analytical method, analytical reagent and analytical instrument using same
WO2012122564A3 (en) Diagnostic and sample preparation devices and methods
WO2013050881A3 (en) Direct nucleic acid analysis

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10729554

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 10729554

Country of ref document: EP

Kind code of ref document: A2