WO2010060176A1 - Automated inoculation mechanism with intermittent stirring system - Google Patents
Automated inoculation mechanism with intermittent stirring system Download PDFInfo
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- WO2010060176A1 WO2010060176A1 PCT/BR2009/000401 BR2009000401W WO2010060176A1 WO 2010060176 A1 WO2010060176 A1 WO 2010060176A1 BR 2009000401 W BR2009000401 W BR 2009000401W WO 2010060176 A1 WO2010060176 A1 WO 2010060176A1
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- blood
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- culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
Definitions
- the medium used to detect and identify the disease and the etiological agent present in the patient's blood is indicated to place the blood in an enriched liquid medium, to allow the proper growth of these agents. This process is carried out due to the small amount of microorganisms always being small. in very severe septicemia.
- these reseedings are done at 24, 48.72 hours of incubation and at 5 and 7 days of incubation in the liquid medium.
- This gas is produced by microbial multiplication, and causes the indicator to change color showing positive blood cultures.
- positive vials should be aspirated with syringes and needles to be sown in solid media which also poses a risk of contamination.
- Another drawback is that not all microorganisms produce enough CO 2 to be detected early. In these cases there may be false negative results or late diagnosis which impairs the patient's treatment. To avoid this problem all bottles must be ressemeados, regardless of the production of C0 2.
- an "AUTOMATED SEEDING MECHANISM WITH INTERMITTENT AGITATION SYSTEM” characterized by a main body formed by a compartment provided with vertically equidistantly spaced guide rails for coupling. trays equipped with several cavities formed by clamps for the packaging of culture cultivation casings that hold a liquid medium. These trays are controlled by a microprocessor programmed to promote its agitation and inclination in order to allow the contact of the sample with the culture at intervals.
- said cabinet is provided with motor, connected to the trays controlled by a panel provided with fuse on / off switch, reversing key, programming key, plus control key, plus control key, display, said panel can be arranged on the panel. lower or upper section below the which has a set of resistors, microventilators and PL lamp, while frontally there is a door, said culture cultivation enclosures having a proper indicator of CO 2 , prepared on a solid surface with agar, which allows a early diagnosis of positivity, and two solid media that allow without the need to aspirate the vials the development of pathogenic bacteria or fungi, so through three indicators of) are two) bacterial growth, (CO 2 production and multiplication in solid media) used in the same equipment which consists of a vial, which medially projects obliquely a collecting duct, and internally said vial holds a liquid medium and superiorly receives a fixed bulb which internally holds three medium solid plates, and a round C0 2 indicator plate below which a chocolate agar plate is
- Figure 1 shows a front view of the equipment alluding to one of the plates of solid medium and the indicator plate C0 2.
- Figure 2 shows a front view of the equipment alluding to one of the solid medium plates.
- AUTOMATED SEED MECHANISM WITH INTERMITTENT AGITATION SYSTEM characterized by a main body (1) formed by an enclosure (2) provided with a housing (3) which holds on the sides (4) vertically spaced guide rails (5), for coupling trays (6) provided with various cavities (7) formed by clips (8) for packaging culture cultivation wrappers (9) which holds a liquid medium (10), said trays (6) controlled by a micro programmed processor
- said cabinet (2) is provided with a motor, connected to the trays (6) controlled by a panel (12) provided with an on / off switch.
- said panel (12) can be arranged in the lower section (21) or upper section (22) below which there is a set of resistors, microventilators and PL lamp, while frontally there is a door (23), said culture cultivation enclosures (9).
- the liquid medium (10) comprising the following formulation BHI or TSB 37g medium, sugar and alcohol 2.5% - 1%, triptone 10g, SPS 10ml, 10ml supplement, deionized water 11, activated carbon 10g / L, aluminum silicate 10g / L, pH 7.8,
- Another advantage of the process in question is the protection afforded to the diagnosis, since the equipment used avoids the contact of the culture with the environment, preventing its contamination through the air, besides protecting the health of the handlers that do not have direct contact with the cultures.
Abstract
Blood culture method for cultivating a patient's blood, and equipment characterised in that, in a single apparatus, an inherent CO2 indicator prepared in a solid agar surface, which allows early diagnosis of seropositivity, is used together with two solid media which allow pathogenic bacteria or fungi to develop without the need to vent the culture bottles. Thus, two bacterial growth indicators (CO2 generation and growth in solid media) are used in the same apparatus, which is formed by a main body in the lower part thereof containing a bottle, from the central part of which an oblique sampling duct projects, said bottle containing a liquid medium and receiving in the upper part thereof a fixed bulb which contains two solid medium plates and one CO2 indicator plate of green colour or of any other colour. In order to facilitate the diagnostic, the manipulator injects blood by means of a syringe into the sampling duct, which leads the blood into the bottle, where the blood is mixed with the liquid medium, then the manipulator turns the apparatus upside down, causing the mixture to fall inside the bulb and come into contact with the plates, whereon two solid media promote the growth of colonies of pathogenic bacteria or fungi, while the CO2 indicator plate changes colour in the presence of the gas. The intermittent stirring process produced in the stove promotes microbial growth and the automated and programmed inversion of the bottles makes inoculation into solid media easier, dispensing with the need for a manipulator in the laboratory for carrying out this step.
Description
"MECANISMO PARA SEMEADURA AUTOMATIZADA COM SISTEMA DE AGITAÇÃO INTERMITENTE". "AUTOMATED SEED MECHANISM WITH INTERMITTENT AGITATION SYSTEM".
Refere-se o presente pedido de patente de invenção a um "MECANISMO PARA SEMEADURA AUTOMATIZADA COM SISTEMA DE AGITAÇÃO INTERMITENTE", que foi desenvolvido visando possibilitar que um único aparelho utilizado contenha dois indicadores de crescimento bacteriano, (produção de CO2 e multiplicação em meios sólidos) o que permite um diagnóstico mais preciso e rápido sem riscos de contaminação da cultura ou do manipulador. This patent application refers to an "AUTOMATED SEEDING MECHANISM WITH INTERMITTENT SHAKE", which has been developed to enable a single apparatus used to contain two indicators of bacterial growth (CO 2 production and multiplication in media). which allows a more accurate and rapid diagnosis without risks of contamination of the crop or the manipulator.
Atualmente o meio utilizado para detectar e identificar a doença e o agente etiológico presente no sangue do paciente é indicado colocar o sangue em um meio líquido enriquecido, para permitir o adequado crescimento destes agentes esse processo e realizado devido à quantidade de microrganismos sempre ser pequena mesmo em septicemias muito graves. Currently, the medium used to detect and identify the disease and the etiological agent present in the patient's blood is indicated to place the blood in an enriched liquid medium, to allow the proper growth of these agents. This process is carried out due to the small amount of microorganisms always being small. in very severe septicemia.
Assim para se saber se existe multiplicação dos microrganismos, é recomendado aspirar com agulha e seringa uma alíquota do meio de cultura líquido em meios sólidos de cultura, com o aparecimento de colónias nestes meios sólidos se terá à confirmação que a hemocultura é positiva. Thus to know if there is multiplication of microorganisms, it is recommended to aspirate with needle and syringe an aliquot of the liquid culture medium in solid culture media, with the appearance of colonies in these solid media will be confirmed that blood culture is positive.
Porem como os agentes podem demorar na sua multiplicação no meio líquido é indicado fazer esta ressemeadura várias vezes após a colocação do sangue no meio. However, as agents may take time to multiply in the liquid medium, it is indicated to do this reseeding several times after placing the blood in the medium.
Em geral estas ressemeaduras são feitas em 24, 48,72 horas de incubação e no 5 e 7 dias da incubação no meio líquido. In general these reseedings are done at 24, 48.72 hours of incubation and at 5 and 7 days of incubation in the liquid medium.
Nos procedimentos atuais um manipulador deve, a intervalos de tempo pré-estabelecidos, efetuar a ressemeadura, a cultura de modo a
permitir que o meio líquido entre em contato com meio sólido que proporciona o aparecimento de colónias. In current procedures a handler should, at pre-established time intervals, reseed, culture in order to Allow the liquid medium to come into contact with solid medium which provides the appearance of colonies.
Percebe-se, portanto que tais procedimentos exigem a presença constante de um operador de laboratório, que pode ocorrer durante a noite, em fins de semana ou feriados. Therefore, it is clear that such procedures require the constant presence of a laboratory operator, which can occur at night, on weekends or holidays.
Outros pontos negativos a destacar estão relacionados ao cultivo de cultura com amostra de pacientes em tratamento com antibióticos, onde a presença do medicamento e presente no sangue, isto pode alterar o resultado do exame dando falsos negativos comprometendo o correio diagnostico e tratamento do doente. Other negative points to highlight are related to culture cultivation with a sample of patients on antibiotic treatment, where the presence of the drug is present in the blood, this can alter the result of the test giving false negatives compromising the patient's diagnostic mail and treatment.
Ocorre que estes sistemas de ressemeaduras representam riscos de contaminações do meio dando resultados falsos positivos, e risco de contaminação do manipulador com os agentes presentes no sangue do paciente. Pois as semeaduras ficam expostas em contato direto com o ar do ambiente que pode altera-las devido as impureza, além de o manipulador poder contrair doenças com o contato acidental com o sangue. It happens that these reseeding systems represent risks of contamination of the environment giving false positive results, and risk of contamination of the handler with the agents present in the patient's blood. For sowing is exposed in direct contact with the ambient air that can change them due to impurity, and the handler can contract diseases with accidental contact with blood.
Algumas modificações deste sistema têm sido feitas para detectai- em forma mais rápida as hemoculturas positivas. Assim os métodos automatizados colocam no interior do frasco com o meio líquido uma membrana indicadora da produção de CO2. Some modifications of this system have been made to detect positive blood cultures more quickly. Thus automated methods place inside the flask with the liquid medium a membrane indicating CO 2 production.
Este gás é produzido pela multiplicação microbiana, e faz com que o indicador mude de cor mostrando as hemoculturas positivas. Porem ainda os frascos positivos devem ser aspirados com seringas e agulha para poder serem semeados em meios sólidos o que também representa riscos de contaminação. Outro inconveniente é que nem todos os microrganismos produzem suficiente quantidade de CO2 para ser detectado em forma precoce. Nestes casos podem existir resultados falsos negativos ou diagnóstico tardios o que prejudica o tratamento do paciente. Para evitar
este problema todos os frascos precisam ser ressemeados, independentemente da produção de C02. This gas is produced by microbial multiplication, and causes the indicator to change color showing positive blood cultures. However, positive vials should be aspirated with syringes and needles to be sown in solid media which also poses a risk of contamination. Another drawback is that not all microorganisms produce enough CO 2 to be detected early. In these cases there may be false negative results or late diagnosis which impairs the patient's treatment. To avoid this problem all bottles must be ressemeados, regardless of the production of C0 2.
Outras modificações têm sido feitas, colocando no mesmo frasco o meio líquido e sólido para evitar assim as ressemeaduras. Porem estes sistemas por não ter indicadores de C02., são mais lentos na indicação de positividade, colocando em risco o tratamento adequado dos pacientes. Other modifications have been made, placing the liquid and solid medium in the same bottle to prevent reseeding. However these systems do not have indicators of C0 2. , are slower in indicating positivity, jeopardizing proper treatment of patients.
Assim, após longo período de estudo o inventor desenvolveu um "MECANISMO PARA SEMEADURA AUTOMATIZADA COM SISTEMA DE AGITAÇÃO INTERMITENTE" caracterizado por um corpo principal, formado por um gabinete provido de compartimento que detém nas laterais réguas guias dispostas verticalmente de forma equidistante, para acoplamento de bandejas dotadas de diversas cavidades formado por presilhas para acondicionamento de invólucros de cultivo de cultura que acondiciona um meio liquido, sendo ditas bandejas controlada por um micro processador programado para promover sua agitação e inclinação de forma a possibilitar o contato da amostra com a cultura em intervalos predeterminados, dito gabinete é provido de motor, conectado as bandejas comandado por um painel provido de chave liga/desliga fusíveis, tecla de reversão, tecla de programação, tecla de controle acréscimo, tecla de controle descréscimo, display, dito painel pode ser disposto na secção inferior ou superior abaixo do qual verifica-se um conjunto de resistências, microventiladores e lâmpada PL, enquanto frontalmente verifica-se uma porta, sendo que dito invólucros de cultivo de cultura dotado de um indicador próprio de CO2, preparado numa superfície sólida com agar, o que permite um diagnóstico precoce da positividade, e de dois meios sólidos que permitem sem necessidade de aspirar os frascos o desenvolvimento de bactérias ou fungos patogênicos, assim através de três indicadores de )são dois) crescimento bacteriano, (produção de CO2 e
multiplicação em meios sólidos) usados no mesmo equipamento esse constituído por um frasco, que medianamente projeta obliquamente um duto coletor, sendo que internamente o dito frasco acondiciona um meio liquido e superiormente recebe de forma fixa um bulbo que internamente detém três placas de meio sólidos, e uma placa indicadora de C02 de forma arredondada, abaixo da qual posiciona uma placa de Agar chocolate para detecção de bactérias e fungos, sendo que na face oposta, projeta-se placas Ágar Sabouraud (para fungos) e Agar Mac Conkey,(para bactérias Gram negativas) sendo o meio liquido composto pela seguinte formulação meio BHI ou TSB 37g, açúcar e álcool 2,5% - 1%, triptone lOg, SPS lOml, suplemento lOml, água deionizada 11, carvão ativado lOg/L, silicato de alumínio lOg L, pH 7,8. Thus, after a long period of study the inventor developed an "AUTOMATED SEEDING MECHANISM WITH INTERMITTENT AGITATION SYSTEM" characterized by a main body formed by a compartment provided with vertically equidistantly spaced guide rails for coupling. trays equipped with several cavities formed by clamps for the packaging of culture cultivation casings that hold a liquid medium. These trays are controlled by a microprocessor programmed to promote its agitation and inclination in order to allow the contact of the sample with the culture at intervals. predetermined, said cabinet is provided with motor, connected to the trays controlled by a panel provided with fuse on / off switch, reversing key, programming key, plus control key, plus control key, display, said panel can be arranged on the panel. lower or upper section below the which has a set of resistors, microventilators and PL lamp, while frontally there is a door, said culture cultivation enclosures having a proper indicator of CO 2 , prepared on a solid surface with agar, which allows a early diagnosis of positivity, and two solid media that allow without the need to aspirate the vials the development of pathogenic bacteria or fungi, so through three indicators of) are two) bacterial growth, (CO 2 production and multiplication in solid media) used in the same equipment which consists of a vial, which medially projects obliquely a collecting duct, and internally said vial holds a liquid medium and superiorly receives a fixed bulb which internally holds three medium solid plates, and a round C0 2 indicator plate below which a chocolate agar plate is located for the detection of bacteria and fungi, and on the opposite side are projected Sabouraud Agar (for fungus) and Mac Conkey Agar (for Gram negative bacteria) the liquid medium comprising the following formulation BHI or TSB 37g medium, sugar and alcohol 2.5% - 1%, triptone 10g, SPS10ml, 10ml supplement, deionized water 11, 10g / L activated carbonate, aluminum 10g L, pH 7.8.
Para que se possa obter uma perfeita compreensão do que fora desenvolvido são apensos desenhos aos quais fazem-se referências numéricas em conjunto com uma descrição pormenorizada, sendo que a: In order to obtain a perfect understanding of what has been developed, drawings are attached to which numerical references are made together with a detailed description:
Figura 1 mostra uma vista frontal do equipamento aludindo uma das placas de meio sólido e a placa de indicadora de C02. Figure 1 shows a front view of the equipment alluding to one of the plates of solid medium and the indicator plate C0 2.
A figura 2 mostra uma vista frontal do equipamento aludindo uma das placas de meio sólido. Figure 2 shows a front view of the equipment alluding to one of the solid medium plates.
De acordo com as ilustrações podemos observar que o According to the illustrations we can observe that the
"MECANISMO PARA SEMEADURA AUTOMATIZADA COM SISTEMA DE AGITAÇÃO INTERMITENTE" caracterizado por um corpo principal (1) formado por um gabinete (2) provido de compartimento (3) que detém nas laterais (4) réguas guias (5) dispostas verticalmente de forma equidistante, para acoplamento de bandejas (6) dotadas de diversas cavidades (7) formado por presilhas (8) para acondicionamento de invólucros de cultivo de cultura (9) que acondiciona um meio liquido (10), sendo ditas bandejas (6) controlada por um micro processador programado
para promover sua agitação e inclinação de forma a possibilitar o contato da amostra com a cultura em intervalos predeterminados, dito gabinete (2) é provido de motor, conectado as bandejas (6) comandado por um painel (12) provido de chave liga/desliga (13) fusíveis (14), tecla de reversão (15), tecla de programação (16), tecla de controle acréscimo (17), tecla de controle descréscimo (18), display (19), dito painel (12) pode ser disposto na secção inferior (21) ou superior (22) abaixo do qual verifica-se um conjunto de resistências, microventiladores e lâmpada PL, enquanto frontalmente verifica-se uma porta (23), sendo que dito invólucros de cultivo de cultura (9) dotado de um indicador próprio de C02, preparado numa superfície sólida com agar, o que permite um diagnóstico precoce da positividade, e de dois meios sólidos que permitem sem necessidade de aspirar os frascos o desenvolvimento de bactérias ou fungos patogênicos, assim através de três indicadores de crescimento bacteriano, (produção de C02 e multiplicação em meios sólidos) usados no mesmo equipamento (9) esse constituído por um frasco (26), que medianamente projeta obliquamente um duto coletor (27), sendo que internamente o dito frasco (26) acondiciona um meio liquido (10) e superiormente recebe de forma fixa um bulbo (29) que internamente detém três placas de meio sólidos (30), e uma placa indicadora de C02 (31) de forma arredondada, abaixo da qual posiciona uma placa de Agar chocolate (32) para detecção de bactérias e fungos, sendo que na face oposta (33), projeta-se placas Agar Sabouraud (para fungos) (34) e Agar Mac Conkey(para bactérias Gram negativas) (35), sendo o meio liquido (10) composto pela seguinte formulação meio BHI ou TSB 37g, açúcar e álcool 2,5% - 1%, triptone lOg, SPS lOml, suplemento lOml, água deionizada 11, carvão ativado lOg/L, silicato de alumínio lOg/L, pH 7,8,
Diante do descrito e ilustrado podemos verificar que o "MECANISMO PARA SEMEADURA AUTOMATIZADA COM SISTEMA DE AGITAÇÃO INTERMITENTE", traz vantagens, pois o mecanismo trata-se de uma incubadora automática desenvolvida com a mais alta tecnologia que proporciona o controle de temperatura em seu interior, com controlador microprocessado de alta precisão e grande estabilidade, cujaas bandejas permitem a acomodação de diversos invólucros que devem ser posicionados de maneira de o local onde é inoculado o sangue fique virado para trás e o laminocultivo com a face dividida do indicador virado para a frente, os invólucros devem ser distribuídos uniformemente , de modo a ocupar a totalidade das cavidades das presilhas, facilitando a circulação do ar aquecido no interior da estufa, a programação fará com que a bandeja seja agitada intermitentemente, havendo uma pausa para que a mesma seja inclinada. Essas etapas ocorrem em um horário predeterminado programado antecipadamente de modo a possibilitar que o meio líquido entre em contato com as placas de cultivo, ocorrendo a semeadura de forma que este sistema elimina por completo a necessidade de se manter um profissional de plantão para efetuar essa operação,reduzindo consequentemente a mão de obra no laboratório. "AUTOMATED SEED MECHANISM WITH INTERMITTENT AGITATION SYSTEM" characterized by a main body (1) formed by an enclosure (2) provided with a housing (3) which holds on the sides (4) vertically spaced guide rails (5), for coupling trays (6) provided with various cavities (7) formed by clips (8) for packaging culture cultivation wrappers (9) which holds a liquid medium (10), said trays (6) controlled by a micro programmed processor To promote its agitation and tilting in order to allow the sample to contact the culture at predetermined intervals, said cabinet (2) is provided with a motor, connected to the trays (6) controlled by a panel (12) provided with an on / off switch. (13) fuses (14), reversing key (15), programming key (16), plus control key (17), plus control key (18), display (19), said panel (12) can be arranged in the lower section (21) or upper section (22) below which there is a set of resistors, microventilators and PL lamp, while frontally there is a door (23), said culture cultivation enclosures (9). provided with its own indicator C0 2, prepared on a solid surface agar, which allows an early diagnosis of positive and two solid media that allow no need to aspirate the bottle the growth of bacteria or fungal pathogens, so through three indicators Bacterial growth (production of C0 2 and multiplication on solid media) used in that equipment (9) that comprises a bottle (26) medially obliquely projects a collecting duct (27), wherein the inside said bottle (26 ) houses a liquid medium (10) and is fixedly receiving a bulb (29) which internally holds three solid medium plates (30), and a rounded C0 2 indicator plate (31) below which it positions a Chocolate agar plate (32) for the detection of bacteria and fungi, and on the opposite side (33), Sabouraud Agar plates (for fungi) (34) and Mac Conkey Agar plates (for Gram negative bacteria) (35) are projected. the liquid medium (10) comprising the following formulation BHI or TSB 37g medium, sugar and alcohol 2.5% - 1%, triptone 10g, SPS 10ml, 10ml supplement, deionized water 11, activated carbon 10g / L, aluminum silicate 10g / L, pH 7.8, Given the described and illustrated we can see that the "AUTOMATED SEED MECHANISM WITH INTERMITTENT SHAKE", brings advantages, because the mechanism is an automatic incubator developed with the highest technology that provides temperature control inside, with high precision microprocessor controller and high stability, whose trays allow the accommodation of several casings that must be positioned so that the place where the blood is inoculated is facing backwards and the laminated culture with the divided face of the indicator facing forward, The casings should be evenly distributed so as to occupy all the cavities of the clamps, facilitating the circulation of the heated air inside the stove. Programming will cause the tray to be shaken intermittently and there is a pause for it to be tilted. These steps occur at a predetermined predetermined time to allow the liquid medium to contact the culture plates, and sowing occurs so that this system completely eliminates the need to maintain a professional on duty to perform this operation. thus reducing labor in the laboratory.
Outra vantagem no processo em questão encontra-se na proteção conferida ao diagnostico, pois o equipamento utilizado evita o contato da cultura com o ambiente impedindo sua contaminação através do ar, além de proteger a saúde dos manipuladores que não tem contato direto com as culturas. Another advantage of the process in question is the protection afforded to the diagnosis, since the equipment used avoids the contact of the culture with the environment, preventing its contamination through the air, besides protecting the health of the handlers that do not have direct contact with the cultures.
Vale salientar ainda que o processo permite um diagnóstico precoce da positividade CO2 sem a necessidade de se utilizar outros equipamentos para cada etapa. Por ser inovador e até então não compreendidas no estado da técnica se enquadra perfeitamente dentro dos
critérios que definem a patente de invenção. Suas reivindicações são as seguintes.
It is also worth noting that the process allows an early diagnosis of CO 2 positivity without the need to use other equipment for each step. Because it is innovative and hitherto not understood in the state of the art, it fits perfectly within the criteria defining the invention patent. Your claims are as follows.
Claims
REIVINDICAÇÃO CLAIM
1 - "MECANISMO PARA SEMEADURA AUTOMATIZADA COM SISTEMA DE AGITAÇÃO INTERMITENTE" caracterizado por um corpo principal (1) formado por um gabinete (2) provido de compartimento (3) que detém nas laterais (4) réguas guias (5) dispostas verticalmente de forma equidistante, para acoplamento de bandejas (6) dotadas de diversas cavidades (7) formado por presilhas (8) para acondicionamento de invólucros de cultivo de cultura (9) que acondiciona um meio liquido (10), sendo ditas bandejas (6) controlada por um micro processador programado para promover sua agitação e inclinação de forma a possibilitar o contato da amostra com a cultura em intervalos predeterminados, dito gabinete (2) é provido de motor, conectado as bandejas (6) comandado por um painel (12) provido de chave liga/desliga (13) fusíveis (14), tecla de reversão (15), tecla de programação (16), tecla de controle acréscimo (17), tecla de controle descréscimo (18), display (19), dito painel (12) pode ser disposto na secção inferior (21) ou superior (22) abaixo do qual verifica-se um conjunto de resistências, microventiladores e lâmpada PL, enquanto frontalmente verifica-se uma porta (23), sendo que dito invólucros de cultivo de cultura (9) dotado de um indicador próprio de CO2, preparado numa superfície sólida com agar, o que permite um diagnóstico precoce da positividade, e de dois meios sólidos que permitem sem necessidade de aspirar os frascos o desenvolvimento de bactérias ou fungos patogênicos, assim através de três indicadores de crescimento bacteriano, (produção de CO2 e multiplicação em meios sólidos) usados no mesmo equipamento (9) esse constituído por um frasco (26), que medianamente projeta obliquamente um duto coletor (27), sendo que internamente o dito frasco (26) acondiciona um meio liquido (10) e superiormente recebe de forma fixa um bulbo (29) que
internamente detém três placas de meio sólidos (30), e uma placa indicadora de CO2 (31) de forma arredondada, abaixo da qual posiciona uma placa de Agar chocolate (32) para detecção de bactérias e fungos, sendo que na face oposta (33), projeta-se placas Ágar Sabouraud (34) e Agar Mac Conkey (35), sendo o meio liquido (10) composto pela seguinte formulação meio BHI ou TSB 37g, açúcar e álcool 2,5% - 1%, triptone lOg, SPS lOml, suplemento lOml, água deionizada 11, carvão ativado lOg/L, silicato de alumínio lOg/L, pH 7,8, 1 - "AUTOMATED SEED MECHANISM WITH INTERMITTENT AGITATION SYSTEM" characterized by a main body (1) formed by an enclosure (2) provided with a housing (3) which has vertically arranged guide rails (5) on the sides (4) equidistant, for coupling trays (6) with several cavities (7) formed by clips (8) for packaging culture cultivation wrappers (9) that holds a liquid medium (10), said trays (6) controlled by a microprocessor programmed to agitate and tilt so as to enable the sample to contact the culture at predetermined intervals, said cabinet (2) is provided with a motor, connected to the trays (6) controlled by a panel (12) provided with power switch (13) fuses (14), reversing key (15), programming key (16), increment control key (17), decrement control key (18), display (19), said panel ( 12) may be provided in lower (21) or upper (22) below which there is a set of resistors, microventilators and PL lamp, while frontally there is a door (23), said culture cultivation enclosures (9) having a proper CO 2 indicator prepared on a solid agar surface allowing early diagnosis of positivity, and two solid media that allow the development of bacteria or pathogenic fungi without aspirating bacterial growth (CO 2 production and multiplication in solid media) used in the same equipment (9) which consists of a flask (26), which obliquely projects a collecting duct (27), and internally said flask (26) it packs a liquid medium (10) and superiorly receives a fixed bulb (29) which internally holds three solid medium plates (30), and a rounded CO 2 indicator plate (31) below which positions a chocolate agar plate (32) for detection of bacteria and fungi, and on the opposite side ( 33), Sabouraud Agar (34) and Mac Conkey Agar (35) plates are designed, the liquid medium (10) consisting of the following formulation BHI or TSB 37g medium, sugar and alcohol 2.5% - 1%, triptone 10g , SPS 10ml, 10ml supplement, deionized water 11, 10g / L activated carbon, 10g / L aluminum silicate, pH 7.8,
2 - "PROCESSO DE HEMOCULTURA PARA CULTIVO DO SANGUE DE UM PACIENTE E EQUIPAMENTO UTILIZADO", de acordo com a reivindicação 1, caracterizado por utilizar no mesmo equipamento um indicador próprio de CO2, preparado numa superfície sólida com agar, que permite um diagnóstico precoce da positividade, e de dois meios sólidos que permitem sem necessidade de aspirar os frascos o desenvolvimento de bactérias ou fungos patogênicos. 2. "HEMOCULTURE PROCESS FOR CROPPING BLOOD OF A PATIENT AND USED EQUIPMENT" according to claim 1, characterized in that it utilizes a proprietary CO 2 indicator prepared on a solid agar surface which enables early diagnosis positivity, and two solid media that allow the development of pathogenic bacteria or fungi without aspirating the vials.
3 - "PROCESSO DE HEMOCULTURA PARA CULTIVO DO SANGUE DE UM PACIENTE E EQUIPAMENTO UTILIZADO", de acordo com a reivindicação 1, caracterizado pelo frasco (26) projetar medianamente e obliquamente um duto coletor (27), que finda no frasco (26) que acondiciona internamente um meio liquido (10) e superiormente recebe de forma fixa um bulbo (29) que internamente detém três placas de meio sólidos (30), e uma placa indicadora de CO2 (31) de forma arredondada, abaixo da qual posiciona uma placa de Agar chocolate (32) para detecção de bactérias e fungos, sendo que na face oposta (33), projeta- se placas Ágar Sabouraud (34) e Agar Mac Conkey (35), 3. A "blood-culture process for culturing a patient's blood and equipment used" according to claim 1, characterized in that the vial (26) medially and obliquely projects a collecting duct (27) which ends in the vial (26) it internally packs a liquid medium (10) and upper it fixedly receives a bulb (29) which internally holds three solid medium plates (30), and a rounded CO 2 indicator plate (31) below which it positions a Chocolate Agar plate (32) for the detection of bacteria and fungi, and on the opposite side (33), Sabouraud Agar (34) and Mac Conkey Agar (35) plates are projected.
4 - "PROCESSO DE HEMOCULTURA PARA CULTIVO DO SANGUE DE UM PACIENTE E EQUIPAMENTO UTILIZADO", de acordo com a reivindicação 1, caracterizado pelas bandejas (6) serem
controlada por ura micro processador programado para promover sua agitação e inclinação de forma a possibilitar o contato da amostra com a cultura em intervalos predeterminados. 4. A "blood culture process for a patient's blood and equipment used" according to claim 1, characterized in that the trays (6) are It is controlled by a microprocessor programmed to promote its agitation and tilting to allow the sample to contact the culture at predetermined intervals.
5 - "PROCESSO DE HEMOCULTURA PARA CULTIVO DO SANGUE DE UM PACIENTE E EQUIPAMENTO UTILIZADO", de acordo com a reivindicação 1, caracterizado pelo meio liquido (10) ser composto pela seguinte formulação meio BHI ou TSB 37g, açúcar e álcool 2,5% - 1%, triptone lOg, SPS lOml, suplemento 10ml, água deionizada 11, carvão ativado lOg/L, silicato de alumínio lOg/L, pH 7,8,
A "blood-culture process for a patient's blood and equipment used" according to claim 1, characterized in that the liquid medium (10) is composed of the following formulation BHI medium or TSB 37g, sugar and alcohol 2.5%. - 1%, triptone 10g, SPS 10ml, supplement 10ml, deionized water 11, activated carbon 10g / L, aluminum silicate 10g / L, pH 7.8,
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BRPI0805321-9A BRPI0805321A2 (en) | 2008-11-28 | 2008-11-28 | automated seeding mechanism with intermittent shaking system |
| BRPI0805321-9 | 2008-11-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2010060176A1 true WO2010060176A1 (en) | 2010-06-03 |
Family
ID=42225153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/BR2009/000401 WO2010060176A1 (en) | 2008-11-28 | 2009-11-27 | Automated inoculation mechanism with intermittent stirring system |
Country Status (3)
| Country | Link |
|---|---|
| BR (1) | BRPI0805321A2 (en) |
| PE (1) | PE20120379A1 (en) |
| WO (1) | WO2010060176A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015013792A1 (en) * | 2013-08-02 | 2015-02-05 | Mimica Mimica Igor | System for cultivating microorganisms with double positive result indicator, comprising a liquid medium, a co2 indicator and multiple selective solid hemoculture media with or without antimicrobial and obligate anaerobic bacteria inhibitors, for mycobacteria |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0304501A (en) * | 2003-04-16 | 2005-05-10 | Igor Mimica Mimica | Greenhouse with stirring and rotation mechanisms for blood cultures under analysis |
-
2008
- 2008-11-28 BR BRPI0805321-9A patent/BRPI0805321A2/en not_active Application Discontinuation
-
2009
- 2009-11-27 PE PE2011001118A patent/PE20120379A1/en not_active Application Discontinuation
- 2009-11-27 WO PCT/BR2009/000401 patent/WO2010060176A1/en active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0304501A (en) * | 2003-04-16 | 2005-05-10 | Igor Mimica Mimica | Greenhouse with stirring and rotation mechanisms for blood cultures under analysis |
Non-Patent Citations (6)
| Title |
|---|
| "Copia da pagina obtida na "Internet Archive Wayback Machine" para documentar o conteudo da sitio em 14/06/2007", 14 June 2007 (2007-06-14), Retrieved from the Internet <URL:http://web.archive.org/web/20070614023059/www.prosangue.sp.gov.br/prosangue/actionpesquisa.do?method=buscar> [retrieved on 20100222] * |
| "PROBAC DO BRASIL - Catalogo comercial dos produtos da empresa", Retrieved from the Internet <URL:http://www.probac.com.br/docs/catalogo.pdf> [retrieved on 20100222] * |
| BARNA GA ET AL.: "Evaluation of two different methods for detection of microbial contamination in platelet concentrates", TRANFUSION - ABSTRACT SUPPLEMENT, vol. 45, no. S3, 2005, pages 57A * |
| GAO BARNA ET AL.: "Avaliacao do sistema HEMOBAC TRIFASICO @ no controle de qualidade microbiologico de bolsas de sangue e hemoderivados", Retrieved from the Internet <URL:http://www.prosangue.sp.gov.br/prosangue/arquivos/pesquisa/probachemo2004.pdf> [retrieved on 20100222] * |
| GARCEZ JUNIOR, E ET AL.: "Estudo comparativo dos Sistemas BacT/Alert® e Hemobac Trifasico® para a realização de hemoculturas", REVISTA DO BIOMÉDICO, vol. 54, July 2003 (2003-07-01), Retrieved from the Internet <URL:http:/lwww.crbml.gov.br/bio54/artigocientifico54.asp> [retrieved on 20100222] * |
| PROBAC DO BRASIL PRODUTOS BACTERIOLOGICOS LTDA: "Processo 824805666 para registro da marca HEMOBAC TRIFASICO", 22 October 2002 (2002-10-22), Retrieved from the Internet <URL:http://pesquisa.inpi.gov.br/MarcaPatente/jsp/servimg/servimg.jsp?BasePesquisa=Marcas> [retrieved on 20100222] * |
Also Published As
| Publication number | Publication date |
|---|---|
| PE20120379A1 (en) | 2012-05-14 |
| BRPI0805321A2 (en) | 2011-08-30 |
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